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Protein

Phospholipase A1

Gene

pldA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per monomer. In the dimeric form the Ca2+ is bound by different amino acids with binding of each Ca2+ shared with ligands coming from each monomer. The Ca2+ ion may have a role in catalysis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126Calcium 1; via carbonyl oxygen; in dimeric formBy similarity1
Active sitei162Proton acceptorBy similarity1
Active sitei164NucleophileBy similarity1
Metal bindingi167Calcium 2; via carbonyl oxygen; in dimeric formBy similarity1
Metal bindingi172Calcium 2; in dimeric formBy similarity1
Metal bindingi204Calcium 3; in monomeric formBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1 (EC:3.1.1.32, EC:3.1.1.4)
Alternative name(s):
Detergent-resistant phospholipase A
Short name:
DR-phospholipase A
Outer membrane phospholipase A
Short name:
OM PLA
Short name:
OMPLA
Phosphatidylcholine 1-acylhydrolase
Gene namesi
Name:pldA
Ordered Locus Names:Z5342, ECs4751
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 52PeriplasmicBy similarityAdd BLAST32
Transmembranei53 – 65Beta strandedBy similarityAdd BLAST13
Topological domaini66 – 84ExtracellularBy similarityAdd BLAST19
Transmembranei85 – 99Beta strandedBy similarityAdd BLAST15
Topological domaini100 – 105PeriplasmicBy similarity6
Transmembranei106 – 118Beta strandedBy similarityAdd BLAST13
Topological domaini119 – 128ExtracellularBy similarity10
Transmembranei129 – 148Beta strandedBy similarityAdd BLAST20
Topological domaini149 – 150PeriplasmicBy similarity2
Transmembranei151 – 164Beta strandedBy similarityAdd BLAST14
Topological domaini165 – 173ExtracellularBy similarity9
Transmembranei174 – 186Beta strandedBy similarityAdd BLAST13
Topological domaini187 – 188PeriplasmicBy similarity2
Transmembranei189 – 198Beta strandedBy similarity10
Topological domaini199 – 216ExtracellularBy similarityAdd BLAST18
Transmembranei217 – 223Beta strandedBy similarity7
Topological domaini224 – 225PeriplasmicBy similarity2
Transmembranei226 – 234Beta strandedBy similarity9
Topological domaini235 – 241ExtracellularBy similarity7
Transmembranei242 – 250Beta strandedBy similarity9
Topological domaini251 – 255PeriplasmicBy similarity5
Transmembranei256 – 265Beta strandedBy similarity10
Topological domaini266 – 274ExtracellularBy similarity9
Transmembranei275 – 286Beta strandedBy similarityAdd BLAST12
Topological domaini287 – 289PeriplasmicBy similarity3

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000002198421 – 289Phospholipase A1Add BLAST269

Interactioni

Subunit structurei

Homodimer; dimerization is reversible, and the dimeric form is the active one.By similarity

Protein-protein interaction databases

STRINGi155864.Z5342

Structurei

3D structure databases

ProteinModelPortaliP0A922
SMRiP0A922
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105HDD Bacteria
COG2829 LUCA
HOGENOMiHOG000288150
KOiK01058
OMAiWGGCRSV

Family and domain databases

CDDicd00541 OMPLA, 1 hit
Gene3Di2.40.230.10, 1 hit
InterProiView protein in InterPro
IPR003187 PLipase_A1
IPR036541 PLipase_A1_sf
PANTHERiPTHR40457 PTHR40457, 1 hit
PfamiView protein in Pfam
PF02253 PLA1, 1 hit
PRINTSiPR01486 PHPHLIPASEA1
SUPFAMiSSF56931 SSF56931, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A922-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRTLQGWLLP VFMLPMAVYA QEATVKEVHD APAVRGSIIA NMLQEHDNPF
60 70 80 90 100
TLYPYDTNYL IYTQTSDLNK EAIASYDWAE NARKDEVKFQ LSLAFPLWRG
110 120 130 140 150
ILGPNSVLGA SYTQKSWWQL SNSEESSPFR ETNYEPQLFL GFATDYRFAG
160 170 180 190 200
WTLRDVEMGY NHDSNGRSDP TSRSWNRLYT RLMAENGNWL VEVKPWYVVG
210 220 230 240 250
NTDDNPDITK YMGYYQLKIG YHLGDAVLSA KGQYNWNTGY GGAELGLSYP
260 270 280
ITKHVRLYTQ VYSGYGESLI DYNFNQTRVG VGVMLNDLF
Length:289
Mass (Da):33,163
Last modified:July 19, 2005 - v1
Checksum:iA688AD32AA60F218
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG59017.1
BA000007 Genomic DNA Translation: BAB38174.1
PIRiE86069
G91222
RefSeqiNP_312778.1, NC_002695.1
WP_001259700.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG59017; AAG59017; Z5342
BAB38174; BAB38174; BAB38174
GeneIDi915153
KEGGiece:Z5342
ecs:ECs4751
PATRICifig|386585.9.peg.4960

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG59017.1
BA000007 Genomic DNA Translation: BAB38174.1
PIRiE86069
G91222
RefSeqiNP_312778.1, NC_002695.1
WP_001259700.1, NZ_NOKN01000002.1

3D structure databases

ProteinModelPortaliP0A922
SMRiP0A922
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5342

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59017; AAG59017; Z5342
BAB38174; BAB38174; BAB38174
GeneIDi915153
KEGGiece:Z5342
ecs:ECs4751
PATRICifig|386585.9.peg.4960

Phylogenomic databases

eggNOGiENOG4105HDD Bacteria
COG2829 LUCA
HOGENOMiHOG000288150
KOiK01058
OMAiWGGCRSV

Family and domain databases

CDDicd00541 OMPLA, 1 hit
Gene3Di2.40.230.10, 1 hit
InterProiView protein in InterPro
IPR003187 PLipase_A1
IPR036541 PLipase_A1_sf
PANTHERiPTHR40457 PTHR40457, 1 hit
PfamiView protein in Pfam
PF02253 PLA1, 1 hit
PRINTSiPR01486 PHPHLIPASEA1
SUPFAMiSSF56931 SSF56931, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPA1_ECO57
AccessioniPrimary (citable) accession number: P0A922
Secondary accession number(s): P00631
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 19, 2005
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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