UniProtKB - P0A912 (PAL_ECOLI)

BLAST

>sp|P0A912|PAL_ECOLI Peptidoglycan-associated lipoprotein OS=Escherichia coli (strain K12) OX=83333 GN=pal PE=1 SV=1
MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMDANGGNGNMSSEEQARL
QMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYKVTVEGHADERGTPEYNIS
LGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAAYSKNRRAVLVY

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History
Entry version 109 (07 Nov 2018)
Sequence version 1 (01 Apr 1988)
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Protein

Peptidoglycan-associated lipoprotein

Gene

pal

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:11115123, PubMed:17233825). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).3 Publications

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division
Source: EcoCyc
Inferred from direct assayⁱ
- 17233825

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division

Biological process

Cell cycle, Cell division

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10684-MONOMER

BioCycⁱ

EcoCyc:EG10684-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Peptidoglycan-associated lipoprotein1 PublicationUniRule annotation Short name: PALUniRule annotationCurated Alternative name(s): Tol-Pal system lipoprotein PalCurated
Gene namesⁱ	Name:pal1 PublicationUniRule annotation Synonyms:excC1 Publication Ordered Locus Names:b0741, JW0731
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10684 pal

EcoGeneⁱ

EG10684 pal

Subcellular locationⁱ

Cell outer membrane
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02204
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL)."
  Lazzaroni J.-C., Portalier R.
  Mol. Microbiol. 6:735-742(1992) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
; Lipid-anchor
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02204
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL)."
  Lazzaroni J.-C., Portalier R.
  Mol. Microbiol. 6:735-742(1992) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.

Note:

Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity.1 Publication

GO - Cellular componentⁱ

cell division site
Source: EcoCyc
Inferred from direct assayⁱ
- 17233825
cell outer membrane
Source: EcoCyc
Inferred from direct assayⁱ
integral component of membrane Source: InterPro
intrinsic component of periplasmic side of cell outer membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 1574003

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell outer membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	UniRule annotationAdd BLAST		21
ChainⁱPRO_0000020120	22 – 173	Peptidoglycan-associated lipoproteinUniRule annotationAdd BLAST		152

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	22	N-palmitoyl cysteineUniRule annotation1 Publication		1
Lipidationⁱ	22	S-diacylglycerol cysteineUniRule annotation1 Publication		1

Keywords - PTMⁱ

Lipoprotein, Palmitate

Proteomic databases

EPDⁱ	P0A912
PaxDbⁱ	P0A912
PRIDEⁱ	P0A912

Interactionⁱ

Subunit structureⁱ

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). Pal interacts with TolB (PubMed:7744736, PubMed:17375930, PubMed:19696740). Interacts with the C-terminal domain of TolA. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743). Can form a complex with TolB and OmpA (PubMed:9701827).1 Publication6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
lolA	P61316	2	EBI-1124760,EBI-553532
lolB	P61320	2	EBI-1124760,EBI-1122794
lolE	P75958	2	EBI-1124760,EBI-1125190
tolB	P0A855	4	EBI-1124760,EBI-7180728

Protein-protein interaction databases

BioGridⁱ	4260724, 260 interactors
Database of interacting proteins More...DIPⁱ	DIP-51001N
IntActⁱ	P0A912, 8 interactors
Molecular INTeraction database More...MINTⁱ	P0A912
STRINGⁱ	316385.ECDH10B_0808

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	69 – 72	Combined sources	4
Helixⁱ	82 – 84	Combined sources	3
Helixⁱ	85 – 97	Combined sources	13
Beta strandⁱ	103 – 107	Combined sources	5
Beta strandⁱ	111 – 113	Combined sources	3
Helixⁱ	115 – 135	Combined sources	21
Helixⁱ	140 – 142	Combined sources	3
Beta strandⁱ	143 – 147	Combined sources	5
Helixⁱ	160 – 166	Combined sources	7
Beta strandⁱ	167 – 172	Combined sources	6

Show more details

3D structure databases

ProteinModelPortalⁱ	P0A912
SMRⁱ	P0A912
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0A912

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	60 – 173	OmpA-likeUniRule annotationAdd BLAST		114

Sequence similaritiesⁱ

Belongs to the OmpA family. Pal subfamily.UniRule annotationCurated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG4105KCK Bacteria COG2885 LUCA
HOGENOMⁱ	HOG000189913
InParanoidⁱ	P0A912
KEGG Orthology (KO) More...KOⁱ	K03640
PhylomeDBⁱ	P0A912

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07185 OmpA_C-like, 1 hit
Gene3Dⁱ	3.30.1330.60, 1 hit
HAMAPⁱ	MF_02204 Pal, 1 hit
InterProⁱ	View protein in InterPro IPR006664 OMP_bac IPR006665 OmpA-like IPR006690 OMPA-like_CS IPR036737 OmpA-like_sf IPR039001 Pal IPR014169 Pal_lipo_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00691 OmpA, 1 hit
PRINTSⁱ	PR01021 OMPADOMAIN
SUPFAMⁱ	SSF103088 SSF103088, 1 hit
TIGRFAMsⁱ	TIGR02802 Pal_lipo, 1 hit
PROSITEⁱ	View protein in PROSITE PS01068 OMPA_1, 1 hit PS51123 OMPA_2, 1 hit PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0A912-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQLNKVLKGL MIALPVMAIA ACSSNKNASN DGSEGMLGAG TGMDANGGNG 
        60         70         80         90        100
NMSSEEQARL QMQQLQQNNI VYFDLDKYDI RSDFAQMLDA HANFLRSNPS 
       110        120        130        140        150
YKVTVEGHAD ERGTPEYNIS LGERRANAVK MYLQGKGVSA DQISIVSYGK 
       160        170 
EKPAVLGHDE AAYSKNRRAV LVY

Length:173

Mass (Da):18,824

Last modified:April 1, 1988 - v1

Checksum:ⁱ449F9959C0274430

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05123 Genomic DNA Translation: CAA28771.1 X65796 Genomic DNA Translation: CAA46673.1 U00096 Genomic DNA Translation: AAC73835.1 AP009048 Genomic DNA Translation: BAA35407.1 M28232 Genomic DNA No translation available.
PIRⁱ	A27534 LPECPG
NCBI Reference Sequences More...RefSeqⁱ	NP_415269.1, NC_000913.3 WP_001295306.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73835; AAC73835; b0741 BAA35407; BAA35407; BAA35407
GeneIDⁱ	945004
KEGGⁱ	ecj:JW0731 eco:b0741
PATRICⁱ	fig\|1411691.4.peg.1531

Protein	Similar proteins		Species	Score	Length	Source
P0A912	Peptidoglycan-associated lipoprotein		ECO57		173	UniRef100_P0A913
Peptidoglycan-associated lipoprotein		SHIFL		173
Peptidoglycan-associated protein		Escherichia coli H591		181
Peptidoglycan-associated protein		Escherichia coli H605		181
Peptidoglycan-associated protein		Escherichia coli H386		181
+167

Protein	Similar proteins		Species	Score	Length	Source
P0A912	Peptidoglycan-associated lipoprotein		ECO57		173	UniRef90_P0A913
Peptidoglycan-associated lipoprotein		SHIFL		173
Peptidoglycan-associated protein		Escherichia coli H591		181
Peptidoglycan-associated protein		Escherichia coli H605		181
Peptidoglycan-associated protein		Escherichia coli H386		181
+709

Protein	Similar proteins		Species	Score	Length	Source
P0A912	Peptidoglycan-associated lipoprotein		ECO57		173	UniRef50_P0A913
Peptidoglycan-associated lipoprotein		SHIFL		173
Peptidoglycan-associated protein		Escherichia coli H591		181
Peptidoglycan-associated protein		Escherichia coli H605		181
Peptidoglycan-associated protein		Escherichia coli H386		181
+1061

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05123 Genomic DNA Translation: CAA28771.1 X65796 Genomic DNA Translation: CAA46673.1 U00096 Genomic DNA Translation: AAC73835.1 AP009048 Genomic DNA Translation: BAA35407.1 M28232 Genomic DNA No translation available.
PIRⁱ	A27534 LPECPG
RefSeqⁱ	NP_415269.1, NC_000913.3 WP_001295306.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1OAP	X-ray	1.93	A	65-173	[»]
	2HQS	X-ray	1.50	C/E/G/H	65-173	[»]
	2W8B	X-ray	1.86	C/E/G/H	65-173	[»]
ProteinModelPortalⁱ	P0A912
SMRⁱ	P0A912
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260724, 260 interactors
DIPⁱ	DIP-51001N
IntActⁱ	P0A912, 8 interactors
MINTⁱ	P0A912
STRINGⁱ	316385.ECDH10B_0808

Proteomic databases

EPDⁱ	P0A912
PaxDbⁱ	P0A912
PRIDEⁱ	P0A912

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73835; AAC73835; b0741 BAA35407; BAA35407; BAA35407
GeneIDⁱ	945004
KEGGⁱ	ecj:JW0731 eco:b0741
PATRICⁱ	fig\|1411691.4.peg.1531

Organism-specific databases

EchoBASEⁱ	EB0678
EcoGeneⁱ	EG10684 pal

Phylogenomic databases

eggNOGⁱ	ENOG4105KCK Bacteria COG2885 LUCA
HOGENOMⁱ	HOG000189913
InParanoidⁱ	P0A912
KOⁱ	K03640
PhylomeDBⁱ	P0A912

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10684-MONOMER

BioCycⁱ

EcoCyc:EG10684-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0A912
Protein Ontology More...PROⁱ	PR:P0A912

Family and domain databases

CDDⁱ	cd07185 OmpA_C-like, 1 hit
Gene3Dⁱ	3.30.1330.60, 1 hit
HAMAPⁱ	MF_02204 Pal, 1 hit
InterProⁱ	View protein in InterPro IPR006664 OMP_bac IPR006665 OmpA-like IPR006690 OMPA-like_CS IPR036737 OmpA-like_sf IPR039001 Pal IPR014169 Pal_lipo_C
Pfamⁱ	View protein in Pfam PF00691 OmpA, 1 hit
PRINTSⁱ	PR01021 OMPADOMAIN
SUPFAMⁱ	SSF103088 SSF103088, 1 hit
TIGRFAMsⁱ	TIGR02802 Pal_lipo, 1 hit
PROSITEⁱ	View protein in PROSITE PS01068 OMPA_1, 1 hit PS51123 OMPA_2, 1 hit PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PAL_ECOLI
Accessionⁱ	P0A912Primary (citable) accession number: P0A912 Secondary accession number(s): P07176
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	April 1, 1988
	Last modified:	November 7, 2018
	This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A912 (PAL_ECOLI)

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Peptidoglycan-associated lipoprotein

pal

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ