UniProtKB - P0A912 (PAL_ECOLI)
Peptidoglycan-associated lipoprotein
pal
Functioni
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:11115123, PubMed:17233825).
The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).
The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098).
4 PublicationsGO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: EcoCyc
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10684-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidoglycan-associated lipoprotein1 PublicationUniRule annotationShort name: PALUniRule annotationCurated Alternative name(s): Tol-Pal system lipoprotein PalCurated |
Gene namesi | Name:pal1 PublicationUniRule annotation Synonyms:excC1 Publication Ordered Locus Names:b0741, JW0731 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane UniRule annotation1 Publication; Lipid-anchor UniRule annotation1 Publication
Note: Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity.1 Publication
Other locations
- cell division site Source: EcoCyc
- cell outer membrane Source: EcoCyc
- integral component of membrane Source: InterPro
- intrinsic component of periplasmic side of cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | UniRule annotationAdd BLAST | 21 | |
ChainiPRO_0000020120 | 22 – 173 | Peptidoglycan-associated lipoproteinUniRule annotationAdd BLAST | 152 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 22 | N-palmitoyl cysteineUniRule annotation1 Publication | 1 | |
Lipidationi | 22 | S-diacylglycerol cysteineUniRule annotation1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0A912 |
PaxDbi | P0A912 |
PRIDEi | P0A912 |
Interactioni
Subunit structurei
The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). Pal interacts with TolB (PubMed:7744736, PubMed:17375930, PubMed:19696740).
Interacts with the C-terminal domain of TolA. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743). Can form a complex with TolB and OmpA (PubMed:9701827).
1 Publication6 PublicationsBinary interactionsi
P0A912
With | #Exp. | IntAct |
---|---|---|
lolA [P61316] | 2 | EBI-1124760,EBI-553532 |
lolB [P61320] | 2 | EBI-1124760,EBI-1122794 |
lolE [P75958] | 2 | EBI-1124760,EBI-1125190 |
tolB [P0A855] | 4 | EBI-1124760,EBI-7180728 |
Protein-protein interaction databases
BioGRIDi | 4260724, 260 interactors 849398, 1 interactor |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-51001N |
IntActi | P0A912, 9 interactors |
MINTi | P0A912 |
STRINGi | 511145.b0741 |
Structurei
Secondary structure
3D structure databases
SMRi | P0A912 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A912 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 60 – 173 | OmpA-likeUniRule annotationAdd BLAST | 114 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 58 | DisorderedSequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2885, Bacteria |
HOGENOMi | CLU_016890_9_4_6 |
InParanoidi | P0A912 |
PhylomeDBi | P0A912 |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
HAMAPi | MF_02204, Pal, 1 hit |
InterProi | View protein in InterPro IPR006664, OMP_bac IPR006665, OmpA-like IPR006690, OMPA-like_CS IPR036737, OmpA-like_sf IPR039001, Pal IPR014169, Pal_lipo_C |
Pfami | View protein in Pfam PF00691, OmpA, 1 hit |
PRINTSi | PR01021, OMPADOMAIN |
SUPFAMi | SSF103088, SSF103088, 1 hit |
TIGRFAMsi | TIGR02802, Pal_lipo, 1 hit |
PROSITEi | View protein in PROSITE PS01068, OMPA_1, 1 hit PS51123, OMPA_2, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MQLNKVLKGL MIALPVMAIA ACSSNKNASN DGSEGMLGAG TGMDANGGNG
60 70 80 90 100
NMSSEEQARL QMQQLQQNNI VYFDLDKYDI RSDFAQMLDA HANFLRSNPS
110 120 130 140 150
YKVTVEGHAD ERGTPEYNIS LGERRANAVK MYLQGKGVSA DQISIVSYGK
160 170
EKPAVLGHDE AAYSKNRRAV LVY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05123 Genomic DNA Translation: CAA28771.1 X65796 Genomic DNA Translation: CAA46673.1 U00096 Genomic DNA Translation: AAC73835.1 AP009048 Genomic DNA Translation: BAA35407.1 M28232 Genomic DNA No translation available. |
PIRi | A27534, LPECPG |
RefSeqi | NP_415269.1, NC_000913.3 WP_001295306.1, NZ_STEB01000035.1 |
Genome annotation databases
EnsemblBacteriai | AAC73835; AAC73835; b0741 BAA35407; BAA35407; BAA35407 |
GeneIDi | 66670989 945004 |
KEGGi | ecj:JW0731 eco:b0741 |
PATRICi | fig|1411691.4.peg.1531 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05123 Genomic DNA Translation: CAA28771.1 X65796 Genomic DNA Translation: CAA46673.1 U00096 Genomic DNA Translation: AAC73835.1 AP009048 Genomic DNA Translation: BAA35407.1 M28232 Genomic DNA No translation available. |
PIRi | A27534, LPECPG |
RefSeqi | NP_415269.1, NC_000913.3 WP_001295306.1, NZ_STEB01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OAP | X-ray | 1.93 | A | 65-173 | [»] | |
2HQS | X-ray | 1.50 | C/E/G/H | 65-173 | [»] | |
2W8B | X-ray | 1.86 | C/E/G/H | 65-173 | [»] | |
SMRi | P0A912 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260724, 260 interactors 849398, 1 interactor |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-51001N |
IntActi | P0A912, 9 interactors |
MINTi | P0A912 |
STRINGi | 511145.b0741 |
Proteomic databases
jPOSTi | P0A912 |
PaxDbi | P0A912 |
PRIDEi | P0A912 |
Genome annotation databases
EnsemblBacteriai | AAC73835; AAC73835; b0741 BAA35407; BAA35407; BAA35407 |
GeneIDi | 66670989 945004 |
KEGGi | ecj:JW0731 eco:b0741 |
PATRICi | fig|1411691.4.peg.1531 |
Organism-specific databases
EchoBASEi | EB0678 |
Phylogenomic databases
eggNOGi | COG2885, Bacteria |
HOGENOMi | CLU_016890_9_4_6 |
InParanoidi | P0A912 |
PhylomeDBi | P0A912 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10684-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A912 |
PROi | PR:P0A912 |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
HAMAPi | MF_02204, Pal, 1 hit |
InterProi | View protein in InterPro IPR006664, OMP_bac IPR006665, OmpA-like IPR006690, OMPA-like_CS IPR036737, OmpA-like_sf IPR039001, Pal IPR014169, Pal_lipo_C |
Pfami | View protein in Pfam PF00691, OmpA, 1 hit |
PRINTSi | PR01021, OMPADOMAIN |
SUPFAMi | SSF103088, SSF103088, 1 hit |
TIGRFAMsi | TIGR02802, Pal_lipo, 1 hit |
PROSITEi | View protein in PROSITE PS01068, OMPA_1, 1 hit PS51123, OMPA_2, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAL_ECOLI | |
Accessioni | P0A912Primary (citable) accession number: P0A912 Secondary accession number(s): P07176 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families