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Protein

Outer membrane protein assembly factor BamC

Gene

bamC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex that stabilizes the interaction between the essential proteins BamA and BamD. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).7 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoliWiki
  • protein insertion into membrane Source: UniProtKB-UniRule

Enzyme and pathway databases

BioCyciEcoCyc:EG10658-MONOMER

Protein family/group databases

TCDBi1.B.33.1.3 the outer membrane protein insertion porin (bam complex) (ompip) family

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein assembly factor BamCUniRule annotation
Gene namesi
Name:bamCUniRule annotation
Synonyms:dapX, nlpB
Ordered Locus Names:b2477, JW2462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10658 bamC

Subcellular locationi

GO - Cellular componenti

  • Bam protein complex Source: EcoCyc
  • cell surface Source: EcoCyc

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000001802725 – 344Outer membrane protein assembly factor BamCAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi25N-palmitoyl cysteineUniRule annotation1 Publication1 Publication1
Lipidationi25S-diacylglycerol cysteineUniRule annotation1 Publication1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0A903
PaxDbiP0A903
PRIDEiP0A903

2D gel databases

SWISS-2DPAGEiP0A903

Interactioni

Subunit structurei

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Forms a subcomplex with BamD and BamE. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262898, 311 interactors
ComplexPortaliCPX-1923 Bam complex
DIPiDIP-39898N
IntActiP0A903, 8 interactors
STRINGi316385.ECDH10B_2643

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A903
SMRiP0A903
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A903

Family & Domainsi

Domaini

Contains two well-defined domains connected by a flexible linker. The C-terminal domain may serve as an important protein-binding surface for interaction with other Bam components or substrates. In addition, contains a long unstructured N-terminal region, which is required to stabilize the BamCD complex. Only the N-terminus of this protein is observed in the Bam complex in X-ray or EM structures, most of the protein must be highly mobile (PubMed:26900875, PubMed:26901871, PubMed:27686148, PubMed:26744406).7 Publications

Sequence similaritiesi

Belongs to the BamC family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107EG0 Bacteria
COG3317 LUCA
HOGENOMiHOG000123040
KOiK07287

Family and domain databases

HAMAPiMF_00924 OM_assembly_BamC, 1 hit
InterProiView protein in InterPro
IPR014524 BamC
IPR010653 NlpB/DapX
PfamiView protein in Pfam
PF06804 Lipoprotein_18, 1 hit
PIRSFiPIRSF026343 NlpB, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A903-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAYSVQKSRL AKVAGVSLVL LLAACSSDSR YKRQVSGDEA YLEAAPLAEL
60 70 80 90 100
HAPAGMILPV TSGDYAIPVT NGSGAVGKAL DIRPPAQPLA LVSGARTQFT
110 120 130 140 150
GDTASLLVEN GRGNTLWPQV VSVLQAKNYT ITQRDDAGQT LTTDWVQWNR
160 170 180 190 200
LDEDEQYRGR YQISVKPQGY QQAVTVKLLN LEQAGKPVAD AASMQRYSTE
210 220 230 240 250
MMNVISAGLD KSATDAANAA QNRASTTMDV QSAADDTGLP MLVVRGPFNV
260 270 280 290 300
VWQRLPAALE KVGMKVTDST RSQGNMAVTY KPLSDSDWQE LGASDPGLAS
310 320 330 340
GDYKLQVGDL DNRSSLQFID PKGHTLTQSQ NDALVAVFQA AFSK
Length:344
Mass (Da):36,842
Last modified:July 19, 2005 - v1
Checksum:i49991F277D9D923C
GO

Sequence cautioni

The sequence M33928 differs from that shown. Reason: Frameshift at positions 50, 64, 81 and 134.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219A → R in M33928 (PubMed:2120198).Curated1

Mass spectrometryi

Molecular mass is 35163 Da from positions 25 - 344. Determined by ESI. With 3 palmitic acid (C16) acyl chains.1 Publication
Molecular mass is 35183 Da from positions 25 - 344. Determined by ESI. With 2 palmitic (C16) and 1 stearic (C18) acid acyl chains.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57402 Genomic DNA Translation: CAA40661.1
M33928 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC75530.2
AP009048 Genomic DNA Translation: BAA16354.1
PIRiD65023
RefSeqiNP_416972.4, NC_000913.3
WP_001297320.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75530; AAC75530; b2477
BAA16354; BAA16354; BAA16354
GeneIDi946954
KEGGiecj:JW2462
eco:b2477
PATRICifig|1411691.4.peg.4262

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57402 Genomic DNA Translation: CAA40661.1
M33928 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC75530.2
AP009048 Genomic DNA Translation: BAA16354.1
PIRiD65023
RefSeqiNP_416972.4, NC_000913.3
WP_001297320.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LAENMR-A101-344[»]
2LAFNMR-A101-344[»]
2YH5X-ray1.25A226-344[»]
2YH6X-ray1.55A/B/C/D101-212[»]
3SNSX-ray1.50A224-343[»]
3TGOX-ray2.90C/D26-344[»]
5AYWX-ray3.56C33-88[»]
5D0OX-ray2.90C1-344[»]
5D0QX-ray3.50C/G1-344[»]
5EKQX-ray3.39C25-344[»]
5LJOelectron microscopy4.90C25-189[»]
ProteinModelPortaliP0A903
SMRiP0A903
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262898, 311 interactors
ComplexPortaliCPX-1923 Bam complex
DIPiDIP-39898N
IntActiP0A903, 8 interactors
STRINGi316385.ECDH10B_2643

Protein family/group databases

TCDBi1.B.33.1.3 the outer membrane protein insertion porin (bam complex) (ompip) family

2D gel databases

SWISS-2DPAGEiP0A903

Proteomic databases

EPDiP0A903
PaxDbiP0A903
PRIDEiP0A903

Protocols and materials databases

DNASUi946954
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75530; AAC75530; b2477
BAA16354; BAA16354; BAA16354
GeneIDi946954
KEGGiecj:JW2462
eco:b2477
PATRICifig|1411691.4.peg.4262

Organism-specific databases

EchoBASEiEB0652
EcoGeneiEG10658 bamC

Phylogenomic databases

eggNOGiENOG4107EG0 Bacteria
COG3317 LUCA
HOGENOMiHOG000123040
KOiK07287

Enzyme and pathway databases

BioCyciEcoCyc:EG10658-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A903
PROiPR:P0A903

Family and domain databases

HAMAPiMF_00924 OM_assembly_BamC, 1 hit
InterProiView protein in InterPro
IPR014524 BamC
IPR010653 NlpB/DapX
PfamiView protein in Pfam
PF06804 Lipoprotein_18, 1 hit
PIRSFiPIRSF026343 NlpB, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBAMC_ECOLI
AccessioniPrimary (citable) accession number: P0A903
Secondary accession number(s): P21167, P76564
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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