UniProtKB - P0A903 (BAMC_ECOLI)
Protein
Outer membrane protein assembly factor BamC
Gene
bamC
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex that stabilizes the interaction between the essential proteins BamA and BamD. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).7 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- Gram-negative-bacterium-type cell outer membrane assembly Source: EcoliWiki
- protein insertion into membrane Source: UniProtKB-UniRule
Enzyme and pathway databases
BioCyci | EcoCyc:EG10658-MONOMER |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Outer membrane protein assembly factor BamCUniRule annotation |
Gene namesi | Name:bamCUniRule annotation Synonyms:dapX, nlpB Ordered Locus Names:b2477, JW2462 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane UniRule annotation3 Publications; Lipid-anchor UniRule annotation2 Publications
GO - Cellular componenti
- Bam protein complex Source: EcoCyc
- cell surface Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Add BLAST | 24 | |
ChainiPRO_0000018027 | 25 – 344 | Outer membrane protein assembly factor BamCAdd BLAST | 320 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 25 | N-palmitoyl cysteineUniRule annotation1 Publication1 Publication | 1 | |
Lipidationi | 25 | S-diacylglycerol cysteineUniRule annotation1 Publication1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0A903 |
PaxDbi | P0A903 |
PRIDEi | P0A903 |
2D gel databases
SWISS-2DPAGEi | P0A903 |
Interactioni
Subunit structurei
Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE.
Forms a subcomplex with BamD and BamE. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).
9 PublicationsBinary interactionsi
Hide detailsP0A903
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-1129043,EBI-1129043 |
bamD [P0AC02] | 4 | EBI-1129043,EBI-1128087 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262898, 311 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-39898N |
IntActi | P0A903, 8 interactors |
STRINGi | 511145.b2477 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0A903 |
SMRi | P0A903 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A903 |
Family & Domainsi
Domaini
Contains two well-defined domains connected by a flexible linker. The C-terminal domain may serve as an important protein-binding surface for interaction with other Bam components or substrates. In addition, contains a long unstructured N-terminal region, which is required to stabilize the BamCD complex. Only the N-terminus of this protein is observed in the Bam complex in X-ray or EM structures, most of the protein must be highly mobile (PubMed:26900875, PubMed:26901871, PubMed:27686148, PubMed:26744406).7 Publications
Sequence similaritiesi
Belongs to the BamC family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3317, Bacteria |
HOGENOMi | CLU_063217_1_0_6 |
Family and domain databases
DisProti | DP01339 |
Gene3Di | 3.30.310.170, 1 hit |
HAMAPi | MF_00924, OM_assembly_BamC, 1 hit |
InterProi | View protein in InterPro IPR014524, BamC IPR042268, BamC_C IPR010653, NlpB/DapX |
Pfami | View protein in Pfam PF06804, Lipoprotein_18, 1 hit |
PIRSFi | PIRSF026343, NlpB, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A903-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYSVQKSRL AKVAGVSLVL LLAACSSDSR YKRQVSGDEA YLEAAPLAEL
60 70 80 90 100
HAPAGMILPV TSGDYAIPVT NGSGAVGKAL DIRPPAQPLA LVSGARTQFT
110 120 130 140 150
GDTASLLVEN GRGNTLWPQV VSVLQAKNYT ITQRDDAGQT LTTDWVQWNR
160 170 180 190 200
LDEDEQYRGR YQISVKPQGY QQAVTVKLLN LEQAGKPVAD AASMQRYSTE
210 220 230 240 250
MMNVISAGLD KSATDAANAA QNRASTTMDV QSAADDTGLP MLVVRGPFNV
260 270 280 290 300
VWQRLPAALE KVGMKVTDST RSQGNMAVTY KPLSDSDWQE LGASDPGLAS
310 320 330 340
GDYKLQVGDL DNRSSLQFID PKGHTLTQSQ NDALVAVFQA AFSK
Sequence cautioni
The sequence M33928 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 219 | A → R in M33928 (PubMed:2120198).Curated | 1 |
Mass spectrometryi
Molecular mass is 35163 Da. Determined by ESI. With 3 palmitic acid (C16) acyl chains.1 Publication
Molecular mass is 35183 Da. Determined by ESI. With 2 palmitic (C16) and 1 stearic (C18) acid acyl chains.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57402 Genomic DNA Translation: CAA40661.1 M33928 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75530.2 AP009048 Genomic DNA Translation: BAA16354.1 |
PIRi | D65023 |
RefSeqi | NP_416972.4, NC_000913.3 WP_001297320.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75530; AAC75530; b2477 BAA16354; BAA16354; BAA16354 |
GeneIDi | 58388530 946954 |
KEGGi | ecj:JW2462 eco:b2477 |
PATRICi | fig|1411691.4.peg.4262 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57402 Genomic DNA Translation: CAA40661.1 M33928 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75530.2 AP009048 Genomic DNA Translation: BAA16354.1 |
PIRi | D65023 |
RefSeqi | NP_416972.4, NC_000913.3 WP_001297320.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LAE | NMR | - | A | 101-344 | [»] | |
2LAF | NMR | - | A | 101-344 | [»] | |
2YH5 | X-ray | 1.25 | A | 226-344 | [»] | |
2YH6 | X-ray | 1.55 | A/B/C/D | 101-212 | [»] | |
3SNS | X-ray | 1.50 | A | 224-343 | [»] | |
3TGO | X-ray | 2.90 | C/D | 26-344 | [»] | |
5AYW | X-ray | 3.56 | C | 33-88 | [»] | |
5D0O | X-ray | 2.90 | C | 1-344 | [»] | |
5D0Q | X-ray | 3.50 | C/G | 1-344 | [»] | |
5EKQ | X-ray | 3.39 | C | 25-344 | [»] | |
5LJO | electron microscopy | 4.90 | C | 25-189 | [»] | |
6SN9 | electron microscopy | 9.80 | C | 25-83 | [»] | |
6V05 | electron microscopy | 4.10 | C | 1-344 | [»] | |
BMRBi | P0A903 | |||||
SMRi | P0A903 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262898, 311 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-39898N |
IntActi | P0A903, 8 interactors |
STRINGi | 511145.b2477 |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
2D gel databases
SWISS-2DPAGEi | P0A903 |
Proteomic databases
jPOSTi | P0A903 |
PaxDbi | P0A903 |
PRIDEi | P0A903 |
Protocols and materials databases
DNASUi | 946954 |
Genome annotation databases
EnsemblBacteriai | AAC75530; AAC75530; b2477 BAA16354; BAA16354; BAA16354 |
GeneIDi | 58388530 946954 |
KEGGi | ecj:JW2462 eco:b2477 |
PATRICi | fig|1411691.4.peg.4262 |
Organism-specific databases
EchoBASEi | EB0652 |
Phylogenomic databases
eggNOGi | COG3317, Bacteria |
HOGENOMi | CLU_063217_1_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10658-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A903 |
PROi | PR:P0A903 |
Family and domain databases
DisProti | DP01339 |
Gene3Di | 3.30.310.170, 1 hit |
HAMAPi | MF_00924, OM_assembly_BamC, 1 hit |
InterProi | View protein in InterPro IPR014524, BamC IPR042268, BamC_C IPR010653, NlpB/DapX |
Pfami | View protein in Pfam PF06804, Lipoprotein_18, 1 hit |
PIRSFi | PIRSF026343, NlpB, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAMC_ECOLI | |
Accessioni | P0A903Primary (citable) accession number: P0A903 Secondary accession number(s): P21167, P76564 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families