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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BioCyciEcoCyc:RPOB-MONOMER
MetaCyc:RPOB-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta (EC:2.7.7.6)
Short name:
RNAP subunit beta
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene namesi
Name:rpoB
Synonyms:groN, nitB, rif, ron, stl, stv, tabD
Ordered Locus Names:b3987, JW3950
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10894 rpoB

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
  • DNA-directed RNA polymerase complex Source: GO_Central
  • membrane Source: UniProtKB

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi813E → K: Disrupts the enzyme's active center. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1852
DrugBankiDB00615 Rifabutin
DB04934 Rifalazil
DB01045 Rifampicin
DB01220 Rifaximin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000478921 – 1342DNA-directed RNA polymerase subunit betaAdd BLAST1342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1022N6-acetyllysine1 Publication1
Modified residuei1200N6-acetyllysine1 Publication1

Post-translational modificationi

Acetylated on several lysine residues in the presence of glucose.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A8V2
PaxDbiP0A8V2
PRIDEiP0A8V2

2D gel databases

SWISS-2DPAGEiP0A8V2

PTM databases

CarbonylDBiP0A8V2
iPTMnetiP0A8V2

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263472, 52 interactors
852782, 1 interactor
DIPiDIP-35777N
IntActiP0A8V2, 103 interactors
STRINGi316385.ECDH10B_4175

Chemistry databases

BindingDBiP0A8V2

Structurei

Secondary structure

11342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 9Combined sources5
Helixi29 – 38Combined sources10
Beta strandi43 – 46Combined sources4
Helixi49 – 56Combined sources8
Beta strandi58 – 60Combined sources3
Beta strandi62 – 75Combined sources14
Helixi82 – 88Combined sources7
Beta strandi93 – 105Combined sources13
Beta strandi113 – 128Combined sources16
Beta strandi136 – 145Combined sources10
Beta strandi147 – 151Combined sources5
Beta strandi154 – 159Combined sources6
Helixi164 – 166Combined sources3
Beta strandi172 – 177Combined sources6
Beta strandi179 – 181Combined sources3
Beta strandi184 – 188Combined sources5
Beta strandi194 – 198Combined sources5
Helixi207 – 212Combined sources6
Helixi217 – 224Combined sources8
Beta strandi227 – 233Combined sources7
Beta strandi236 – 240Combined sources5
Helixi243 – 246Combined sources4
Beta strandi255 – 257Combined sources3
Beta strandi260 – 263Combined sources4
Helixi271 – 279Combined sources9
Beta strandi284 – 286Combined sources3
Helixi289 – 293Combined sources5
Turni304 – 306Combined sources3
Beta strandi309 – 311Combined sources3
Helixi319 – 323Combined sources5
Turni326 – 329Combined sources4
Beta strandi332 – 336Combined sources5
Beta strandi340 – 343Combined sources4
Helixi346 – 353Combined sources8
Helixi359 – 370Combined sources12
Helixi378 – 389Combined sources12
Turni392 – 394Combined sources3
Helixi398 – 403Combined sources6
Turni406 – 410Combined sources5
Helixi424 – 429Combined sources6
Helixi433 – 437Combined sources5
Beta strandi450 – 453Combined sources4
Helixi456 – 481Combined sources26
Beta strandi484 – 487Combined sources4
Turni489 – 491Combined sources3
Helixi496 – 508Combined sources13
Beta strandi512 – 514Combined sources3
Helixi520 – 527Combined sources8
Beta strandi530 – 532Combined sources3
Turni540 – 542Combined sources3
Helixi545 – 548Combined sources4
Helixi552 – 554Combined sources3
Turni555 – 557Combined sources3
Beta strandi565 – 572Combined sources8
Beta strandi587 – 595Combined sources9
Beta strandi598 – 607Combined sources10
Helixi610 – 613Combined sources4
Turni625 – 627Combined sources3
Beta strandi628 – 637Combined sources10
Beta strandi639 – 646Combined sources8
Turni647 – 649Combined sources3
Beta strandi652 – 655Combined sources4
Helixi665 – 667Combined sources3
Helixi671 – 673Combined sources3
Helixi676 – 685Combined sources10
Helixi686 – 688Combined sources3
Beta strandi699 – 701Combined sources3
Helixi705 – 711Combined sources7
Beta strandi715 – 717Combined sources3
Beta strandi722 – 727Combined sources6
Beta strandi729 – 736Combined sources8
Turni738 – 740Combined sources3
Beta strandi748 – 752Combined sources5
Beta strandi756 – 758Combined sources3
Beta strandi762 – 765Combined sources4
Beta strandi782 – 785Combined sources4
Beta strandi796 – 805Combined sources10
Turni808 – 811Combined sources4
Beta strandi812 – 814Combined sources3
Beta strandi816 – 819Combined sources4
Helixi820 – 824Combined sources5
Beta strandi832 – 843Combined sources12
Beta strandi849 – 852Combined sources4
Helixi859 – 863Combined sources5
Beta strandi869 – 871Combined sources3
Beta strandi882 – 884Combined sources3
Beta strandi886 – 889Combined sources4
Helixi892 – 894Combined sources3
Helixi897 – 906Combined sources10
Beta strandi913 – 915Combined sources3
Beta strandi925 – 936Combined sources12
Helixi943 – 980Combined sources38
Turni981 – 983Combined sources3
Helixi986 – 991Combined sources6
Helixi994 – 996Combined sources3
Turni997 – 999Combined sources3
Beta strandi1003 – 1005Combined sources3
Helixi1006 – 1037Combined sources32
Beta strandi1046 – 1056Combined sources11
Beta strandi1065 – 1068Combined sources4
Beta strandi1075 – 1080Combined sources6
Helixi1082 – 1084Combined sources3
Beta strandi1095 – 1098Combined sources4
Helixi1102 – 1105Combined sources4
Helixi1110 – 1133Combined sources24
Helixi1138 – 1150Combined sources13
Helixi1167 – 1176Combined sources10
Turni1186 – 1188Combined sources3
Helixi1192 – 1202Combined sources11
Beta strandi1206 – 1210Combined sources5
Turni1215 – 1217Combined sources3
Beta strandi1225 – 1236Combined sources12
Helixi1239 – 1242Combined sources4
Beta strandi1244 – 1248Combined sources5
Beta strandi1251 – 1255Combined sources5
Beta strandi1261 – 1264Combined sources4
Helixi1272 – 1280Combined sources9
Helixi1285 – 1291Combined sources7
Turni1292 – 1296Combined sources5
Helixi1298 – 1310Combined sources13
Helixi1321 – 1332Combined sources12
Beta strandi1335 – 1338Combined sources4

3D structure databases

ProteinModelPortaliP0A8V2
SMRiP0A8V2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8V2

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Phylogenomic databases

eggNOGiENOG4107QIH Bacteria
COG0085 LUCA
HOGENOMiHOG000218612
InParanoidiP0A8V2
KOiK03043
OMAiFMTWEGY
PhylomeDBiP0A8V2

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 2 hits
2.40.50.150, 1 hit
3.90.1110.10, 2 hits
HAMAPiMF_01321 RNApol_bact_RpoB, 1 hit
InterProiView protein in InterPro
IPR019462 DNA-dir_RNA_pol_bsu_external_1
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR010243 RNA_pol_bsu_bac
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR037034 RNA_pol_Rpb2_2_sf
IPR007645 RNA_pol_Rpb2_3
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 2 hits
PF04565 RNA_pol_Rpb2_3, 1 hit
PF10385 RNA_pol_Rpb2_45, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
TIGRFAMsiTIGR02013 rpoB, 1 hit
PROSITEiView protein in PROSITE
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

P0A8V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE
60 70 80 90 100
AAFRSVFPIQ SYSGNSELQY VSYRLGEPVF DVQECQIRGV TYSAPLRVKL
110 120 130 140 150
RLVIYEREAP EGTVKDIKEQ EVYMGEIPLM TDNGTFVING TERVIVSQLH
160 170 180 190 200
RSPGVFFDSD KGKTHSSGKV LYNARIIPYR GSWLDFEFDP KDNLFVRIDR
210 220 230 240 250
RRKLPATIIL RALNYTTEQI LDLFFEKVIF EIRDNKLQME LVPERLRGET
260 270 280 290 300
ASFDIEANGK VYVEKGRRIT ARHIRQLEKD DVKLIEVPVE YIAGKVVAKD
310 320 330 340 350
YIDESTGELI CAANMELSLD LLAKLSQSGH KRIETLFTND LDHGPYISET
360 370 380 390 400
LRVDPTNDRL SALVEIYRMM RPGEPPTREA AESLFENLFF SEDRYDLSAV
410 420 430 440 450
GRMKFNRSLL REEIEGSGIL SKDDIIDVMK KLIDIRNGKG EVDDIDHLGN
460 470 480 490 500
RRIRSVGEMA ENQFRVGLVR VERAVKERLS LGDLDTLMPQ DMINAKPISA
510 520 530 540 550
AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGPGGLTR ERAGFEVRDV
560 570 580 590 600
HPTHYGRVCP IETPEGPNIG LINSLSVYAQ TNEYGFLETP YRKVTDGVVT
610 620 630 640 650
DEIHYLSAIE EGNYVIAQAN SNLDEEGHFV EDLVTCRSKG ESSLFSRDQV
660 670 680 690 700
DYMDVSTQQV VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLRADKPLV
710 720 730 740 750
GTGMERAVAV DSGVTAVAKR GGVVQYVDAS RIVIKVNEDE MYPGEAGIDI
760 770 780 790 800
YNLTKYTRSN QNTCINQMPC VSLGEPVERG DVLADGPSTD LGELALGQNM
810 820 830 840 850
RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELACVS RDTKLGPEEI
860 870 880 890 900
TADIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK
910 920 930 940 950
LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQVFTRDGVE KDKRALEIEE
960 970 980 990 1000
MQLKQAKKDL SEELQILEAG LFSRIRAVLV AGGVEAEKLD KLPRDRWLEL
1010 1020 1030 1040 1050
GLTDEEKQNQ LEQLAEQYDE LKHEFEKKLE AKRRKITQGD DLAPGVLKIV
1060 1070 1080 1090 1100
KVYLAVKRRI QPGDKMAGRH GNKGVISKIN PIEDMPYDEN GTPVDIVLNP
1110 1120 1130 1140 1150
LGVPSRMNIG QILETHLGMA AKGIGDKINA MLKQQQEVAK LREFIQRAYD
1160 1170 1180 1190 1200
LGADVRQKVD LSTFSDEEVM RLAENLRKGM PIATPVFDGA KEAEIKELLK
1210 1220 1230 1240 1250
LGDLPTSGQI RLYDGRTGEQ FERPVTVGYM YMLKLNHLVD DKMHARSTGS
1260 1270 1280 1290 1300
YSLVTQQPLG GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG
1310 1320 1330 1340
RTKMYKNIVD GNHQMEPGMP ESFNVLLKEI RSLGINIELE DE
Length:1,342
Mass (Da):150,632
Last modified:June 7, 2005 - v1
Checksum:iF9E95344C54AB118
GO

Sequence cautioni

The sequence described in Ref. 7 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → R AA sequence (PubMed:1095419).Curated1
Sequence conflicti106 – 107ER → G in CAA23629 (PubMed:7011900).Curated2
Sequence conflicti384 – 391LFENLFFS → CSRTCSSPT in CAA23629 (PubMed:7011900).Curated8
Sequence conflicti516D → V in CAA23625 (PubMed:6266829).Curated1
Sequence conflicti516D → V in CAA23627 (PubMed:6266829).Curated1
Sequence conflicti516D → V in AAA24585 (Ref. 11) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA Translation: CAA23625.1
V00340 Genomic DNA Translation: CAA23627.1
U76222 Genomic DNA Translation: AAB18647.1
U00006 Genomic DNA Translation: AAC43085.1
U00096 Genomic DNA Translation: AAC76961.1
AP009048 Genomic DNA Translation: BAE77333.1
V00341 Genomic DNA Translation: CAA23629.1
M38292 Genomic DNA Translation: AAA24579.1
M38293 Genomic DNA Translation: AAA24581.1
M38287 Genomic DNA Translation: AAA24585.1
M38304 Genomic DNA Translation: AAA24580.1
U77436 Genomic DNA Translation: AAD09605.1
M38303 Genomic DNA Translation: AAA24583.1
PIRiF65205 RNECB
RefSeqiNP_418414.1, NC_000913.3
WP_000263098.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76961; AAC76961; b3987
BAE77333; BAE77333; BAE77333
GeneIDi948488
KEGGiecj:JW3950
eco:b3987
PATRICifig|1411691.4.peg.2725

Similar proteinsi

Entry informationi

Entry nameiRPOB_ECOLI
AccessioniPrimary (citable) accession number: P0A8V2
Secondary accession number(s): P00575
, P00576, P78242, Q2M8S3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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