UniProtKB - P0A8V0 (RBN_ECOLI)
Protein
Ribonuclease BN
Gene
rbn
Organism
Escherichia coli (strain K12)
Status
Functioni
Zinc phosphodiesterase, which has both exoribonuclease and endoribonuclease activities, depending on the nature of the substrate and of the added divalent cation, and on its 3'-terminal structure. Can process the 3' termini of both CCA-less and CCA-containing tRNA precursors. CCA-less tRNAs are cleaved endonucleolytically after the discriminator base, whereas residues following the CCA sequence can be removed exonucleolytically or endonucleolytically in CCA-containing molecules. Does not remove the CCA sequence. May also be involved in the degradation of mRNAs. In vitro, hydrolyzes bis(p-nitrophenyl)phosphate and thymidine-5'-p-nitrophenyl phosphate.5 Publications
Caution
Was initially thought to be an arylsulfatase, given its similarity with the AtsA family. PubMed:12029081 however showed that it is a zinc phosphodiesterase.Curated
Cofactori
Zn2+1 PublicationNote: Binds 2 Zn2+ ions.1 Publication
Activity regulationi
Activated by cobalt and manganese. Strongly inhibited by the presence of a 3'-CCA sequence or a 3'-phosphoryl group.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 66 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Active sitei | 68 | Proton acceptorUniRule annotation | 1 | |
Metal bindingi | 68 | Zinc 2; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 69 | Zinc 2; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 141 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 212 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 212 | Zinc 2; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 270 | Zinc 2; catalyticUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- 3'-tRNA processing endoribonuclease activity Source: EcoCyc
- endoribonuclease activity, producing 5'-phosphomonoesters Source: EcoCyc
- exoribonuclease activity Source: EcoliWiki
- exoribonuclease activity, producing 5'-phosphomonoesters Source: EcoCyc
- metal ion binding Source: EcoCyc
- nuclease activity Source: EcoliWiki
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- tRNA 3'-trailer cleavage, endonucleolytic Source: GO_Central
- tRNA processing Source: EcoliWiki
Keywordsi
Molecular function | Endonuclease, Exonuclease, Hydrolase, Nuclease |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7175-MONOMER MetaCyc:G7175-MONOMER |
BRENDAi | 3.1.26.11, 2026 |
SABIO-RKi | P0A8V0 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease BNUniRule annotation (EC:3.1.-.-UniRule annotation)Short name: RNase BNUniRule annotation Alternative name(s): Ribonuclease Z homologUniRule annotation Short name: RNase Z homologUniRule annotation |
Gene namesi | Name:rbn Synonyms:elaC, rnz Ordered Locus Names:b2268, JW2263 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants are unaffected in growth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155863 | 1 – 305 | Ribonuclease BNAdd BLAST | 305 |
Proteomic databases
jPOSTi | P0A8V0 |
PaxDbi | P0A8V0 |
PRIDEi | P0A8V0 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4263118, 36 interactors 851101, 1 interactor |
DIPi | DIP-9498N |
IntActi | P0A8V0, 7 interactors |
STRINGi | 511145.b2268 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8V0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8V0 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1234, Bacteria |
HOGENOMi | CLU_031317_2_0_6 |
InParanoidi | P0A8V0 |
PhylomeDBi | P0A8V0 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013469, Rnase_BN IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 2 hits |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit TIGR02649, true_RNase_BN, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8V0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELIFLGTSA GVPTRTRNVT AILLNLQHPT QSGLWLFDCG EGTQHQLLHT
60 70 80 90 100
AFNPGKLDKI FISHLHGDHL FGLPGLLCSR SMSGIIQPLT IYGPQGIREF
110 120 130 140 150
VETALRISGS WTDYPLEIVE IGAGEILDDG LRKVTAYPLE HPLECYGYRI
160 170 180 190 200
EEHDKPGALN AQALKAAGVP PGPLFQELKA GKTITLEDGR QINGADYLAA
210 220 230 240 250
PVPGKALAIF GDTGPCDAAL DLAKGVDVMV HEATLDITME AKANSRGHSS
260 270 280 290 300
TRQAATLARE AGVGKLIITH VSSRYDDKGC QHLLRECRSI FPATELANDF
TVFNV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 154 | D → Y in AAB02732 (Ref. 1) Curated | 1 | |
Sequence conflicti | 217 | D → N in AAB02732 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58768 Genomic DNA Translation: AAB02732.1 U00096 Genomic DNA Translation: AAC75328.2 AP009048 Genomic DNA Translation: BAA16095.2 |
PIRi | B64998 |
RefSeqi | NP_416771.4, NC_000913.3 WP_001300687.1, NZ_STEB01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC75328; AAC75328; b2268 BAA16095; BAA16095; BAA16095 |
GeneIDi | 48134891 946760 |
KEGGi | ecj:JW2263 eco:b2268 |
PATRICi | fig|1411691.4.peg.4468 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58768 Genomic DNA Translation: AAB02732.1 U00096 Genomic DNA Translation: AAC75328.2 AP009048 Genomic DNA Translation: BAA16095.2 |
PIRi | B64998 |
RefSeqi | NP_416771.4, NC_000913.3 WP_001300687.1, NZ_STEB01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CBN | X-ray | 2.90 | A | 1-305 | [»] | |
SMRi | P0A8V0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263118, 36 interactors 851101, 1 interactor |
DIPi | DIP-9498N |
IntActi | P0A8V0, 7 interactors |
STRINGi | 511145.b2268 |
Proteomic databases
jPOSTi | P0A8V0 |
PaxDbi | P0A8V0 |
PRIDEi | P0A8V0 |
Genome annotation databases
EnsemblBacteriai | AAC75328; AAC75328; b2268 BAA16095; BAA16095; BAA16095 |
GeneIDi | 48134891 946760 |
KEGGi | ecj:JW2263 eco:b2268 |
PATRICi | fig|1411691.4.peg.4468 |
Organism-specific databases
EchoBASEi | EB4008 |
Phylogenomic databases
eggNOGi | COG1234, Bacteria |
HOGENOMi | CLU_031317_2_0_6 |
InParanoidi | P0A8V0 |
PhylomeDBi | P0A8V0 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7175-MONOMER MetaCyc:G7175-MONOMER |
BRENDAi | 3.1.26.11, 2026 |
SABIO-RKi | P0A8V0 |
Miscellaneous databases
EvolutionaryTracei | P0A8V0 |
PROi | PR:P0A8V0 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013469, Rnase_BN IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 2 hits |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit TIGR02649, true_RNase_BN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBN_ECOLI | |
Accessioni | P0A8V0Primary (citable) accession number: P0A8V0 Secondary accession number(s): P77449, Q47012 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families