UniProtKB - P0A8R0 (RRAA_ECOLI)
Protein
Regulator of ribonuclease activity A
Gene
rraA
Organism
Escherichia coli (strain K12)
Status
Functioni
Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. Modulates RNA-binding and helicase activities of the degradosome.5 Publications
Miscellaneous
RraA and RraB interact with Rne at separate sites within the Rne and exert distinct effects on the composition of the degradosome, affecting distinct sets of RNA transcripts.
Caution
Although it was initially thought to be a methyltransferase of the menaquinone pathway, PubMed:13678585 showed that it has no SAM-dependent methyltransferase activity and is not involved in the menaquinone pathway.Curated
GO - Molecular functioni
- endoribonuclease inhibitor activity Source: UniProtKB-UniRule
- enzyme binding Source: EcoCyc
- ribonuclease inhibitor activity Source: EcoCyc
GO - Biological processi
- negative regulation of endoribonuclease activity Source: EcoCyc
- negative regulation of RNA catabolic process Source: EcoCyc
- protein homotrimerization Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:EG11879-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of ribonuclease activity A |
Gene namesi | Name:rraA Synonyms:menG, yiiV Ordered Locus Names:b3929, JW3900 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209611 | 1 – 161 | Regulator of ribonuclease activity AAdd BLAST | 161 |
Proteomic databases
jPOSTi | P0A8R0 |
PaxDbi | P0A8R0 |
PRIDEi | P0A8R0 |
Expressioni
Inductioni
Induced upon entry into stationary phase, in a RpoS-dependent manner. Stability of the rraA transcript is Rne dependent, suggesting the existence of a feedback mechanism in the regulation of RraA level.1 Publication
Interactioni
Subunit structurei
Homotrimer.
Forms a ring-like structure with a central cavity. Binds to both RNA-binding sites in the C-terminal region of Rne and to RhlB.
1 PublicationGO - Molecular functioni
- enzyme binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261785, 27 interactors 852716, 3 interactors |
DIPi | DIP-35864N |
IntActi | P0A8R0, 12 interactors |
STRINGi | 511145.b3929 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8R0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8R0 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RraA family.Curated
Phylogenomic databases
eggNOGi | COG0684, Bacteria |
HOGENOMi | CLU_072626_4_0_6 |
InParanoidi | P0A8R0 |
PhylomeDBi | P0A8R0 |
Family and domain databases
CDDi | cd16841, RraA_family, 1 hit |
HAMAPi | MF_00471, RraA, 1 hit |
InterProi | View protein in InterPro IPR010203, RraA IPR005493, RraA/RraA-like IPR036704, RraA/RraA-like_sf IPR014339, RraA_gpbac |
Pfami | View protein in Pfam PF03737, RraA-like, 1 hit |
SUPFAMi | SSF89562, SSF89562, 1 hit |
TIGRFAMsi | TIGR01935, NOT-MenG, 1 hit TIGR02998, RraA_entero, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8R0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKYDTSELCD IYQEDVNVVE PLFSNFGGRA SFGGQIITVK CFEDNGLLYD
60 70 80 90 100
LLEQNGRGRV LVVDGGGSVR RALVDAELAR LAVQNEWEGL VIYGAVRQVD
110 120 130 140 150
DLEELDIGIQ AMAAIPVGAA GEGIGESDVR VNFGGVTFFS GDHLYADNTG
160
IILSEDPLDI E
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56082 Genomic DNA Translation: AAB01208.1 L19201 Genomic DNA Translation: AAB03061.1 U00096 Genomic DNA Translation: AAC76911.1 AP009048 Genomic DNA Translation: BAE77381.1 |
PIRi | S40872 |
RefSeqi | NP_418364.1, NC_000913.3 WP_000872908.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76911; AAC76911; b3929 BAE77381; BAE77381; BAE77381 |
GeneIDi | 58459842 948419 |
KEGGi | ecj:JW3900 eco:b3929 |
PATRICi | fig|1411691.4.peg.2776 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56082 Genomic DNA Translation: AAB01208.1 L19201 Genomic DNA Translation: AAB03061.1 U00096 Genomic DNA Translation: AAC76911.1 AP009048 Genomic DNA Translation: BAE77381.1 |
PIRi | S40872 |
RefSeqi | NP_418364.1, NC_000913.3 WP_000872908.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q5X | X-ray | 2.00 | A/B/C | 1-161 | [»] | |
2YJT | X-ray | 2.90 | A/B/C | 1-161 | [»] | |
2YJV | X-ray | 2.80 | A/B/C/D/E/F/G/H/I/J/K/L | 1-161 | [»] | |
SMRi | P0A8R0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261785, 27 interactors 852716, 3 interactors |
DIPi | DIP-35864N |
IntActi | P0A8R0, 12 interactors |
STRINGi | 511145.b3929 |
Proteomic databases
jPOSTi | P0A8R0 |
PaxDbi | P0A8R0 |
PRIDEi | P0A8R0 |
Genome annotation databases
EnsemblBacteriai | AAC76911; AAC76911; b3929 BAE77381; BAE77381; BAE77381 |
GeneIDi | 58459842 948419 |
KEGGi | ecj:JW3900 eco:b3929 |
PATRICi | fig|1411691.4.peg.2776 |
Organism-specific databases
EchoBASEi | EB1825 |
Phylogenomic databases
eggNOGi | COG0684, Bacteria |
HOGENOMi | CLU_072626_4_0_6 |
InParanoidi | P0A8R0 |
PhylomeDBi | P0A8R0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11879-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A8R0 |
PROi | PR:P0A8R0 |
Family and domain databases
CDDi | cd16841, RraA_family, 1 hit |
HAMAPi | MF_00471, RraA, 1 hit |
InterProi | View protein in InterPro IPR010203, RraA IPR005493, RraA/RraA-like IPR036704, RraA/RraA-like_sf IPR014339, RraA_gpbac |
Pfami | View protein in Pfam PF03737, RraA-like, 1 hit |
SUPFAMi | SSF89562, SSF89562, 1 hit |
TIGRFAMsi | TIGR01935, NOT-MenG, 1 hit TIGR02998, RraA_entero, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RRAA_ECOLI | |
Accessioni | P0A8R0Primary (citable) accession number: P0A8R0 Secondary accession number(s): P32165, Q2M8M5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families