UniProtKB - P0A8Q3 (FRDD_ECOLI)
Protein
Fumarate reductase subunit D
Gene
frdD
Organism
Escherichia coli (strain K12)
Status
Functioni
Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used in anaerobic growth, and succinate dehydrogenase is used in aerobic growth. Anchors the catalytic components of the fumarate reductase complex to the cell inner membrane, binds quinones (PubMed:10373108). The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol-binding sites are functionally relevant (PubMed:10373108, PubMed:11850430).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 15 | MenaquinoneCombined sources | 1 |
GO - Molecular functioni
- fumarate reductase (menaquinone) Source: EcoCyc
- succinate dehydrogenase activity Source: UniProtKB-UniRule
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- bacterial-type flagellum assembly Source: EcoCyc
- fermentation Source: EcoCyc
- fumarate metabolic process Source: InterPro
Enzyme and pathway databases
BioCyci | EcoCyc:FUM-MEMB2 MetaCyc:FUM-MEMB2 |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate reductase subunit DUniRule annotationAlternative name(s): Fumarate reductase 13 kDa hydrophobic proteinUniRule annotation Quinol-fumarate reductase subunit D1 PublicationUniRule annotation Short name: QFR subunit D1 PublicationUniRule annotation |
Gene namesi | Name:frdDUniRule annotation Ordered Locus Names:b4151, JW4112 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
- plasma membrane fumarate reductase complex Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 9 – 35 | Helical1 PublicationAdd BLAST | 27 | |
Topological domaini | 36 – 60 | Periplasmic1 PublicationAdd BLAST | 25 | |
Transmembranei | 61 – 89 | Helical1 PublicationAdd BLAST | 29 | |
Topological domaini | 90 – 96 | Cytoplasmic1 Publication | 7 | |
Transmembranei | 97 – 115 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 116 – 119 | Periplasmic2 Publications | 4 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Chemistry databases
DrugBanki | DB07490, 2-[1-(4-CHLORO-PHENYL)-ETHYL]-4,6-DINITRO-PHENOL DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000196542 | 1 – 119 | Fumarate reductase subunit DAdd BLAST | 119 |
Proteomic databases
jPOSTi | P0A8Q3 |
PaxDbi | P0A8Q3 |
PRIDEi | P0A8Q3 |
Expressioni
Inductioni
Regulated at the transcriptional level in response to the cellular availability of the alternate electron acceptors oxygen, nitrate, and fumarate.1 Publication
Interactioni
Subunit structurei
Part of an enzyme complex containing four subunits: a flavoprotein (FrdA), an iron-sulfur protein (FrdB), and two hydrophobic anchor proteins (FrdC and FrdD).
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262699, 457 interactors |
ComplexPortali | CPX-1967, Plasma membrane fumarate reductase complex |
DIPi | DIP-9684N |
IntActi | P0A8Q3, 1 interactor |
STRINGi | 511145.b4151 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8Q3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8Q3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FrdD family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3080, Bacteria |
HOGENOMi | CLU_168367_0_0_6 |
Family and domain databases
CDDi | cd00547, QFR_TypeD_subunitD, 1 hit |
Gene3Di | 1.20.1300.10, 1 hit |
HAMAPi | MF_00709, Fumarate_red_D, 1 hit |
InterProi | View protein in InterPro IPR003418, Fumarate_red_D IPR034804, SQR/QFR_C/D |
Pfami | View protein in Pfam PF02313, Fumarate_red_D, 1 hit |
PIRSFi | PIRSF000179, FrdD, 1 hit |
SUPFAMi | SSF81343, SSF81343, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8Q3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MINPNPKRSD EPVFWGLFGA GGMWSAIIAP VMILLVGILL PLGLFPGDAL
60 70 80 90 100
SYERVLAFAQ SFIGRVFLFL MIVLPLWCGL HRMHHAMHDL KIHVPAGKWV
110
FYGLAAILTV VTLIGVVTI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01611 Genomic DNA Translation: AAA23440.1 V00277 Genomic DNA Translation: CAA23536.1 U14003 Genomic DNA Translation: AAA97050.1 U00096 Genomic DNA Translation: AAC77111.1 AP009048 Genomic DNA Translation: BAE78155.1 M11979 Genomic DNA Translation: AAA23434.1 |
PIRi | A04431, WMEC13 |
RefSeqi | NP_418575.1, NC_000913.3 WP_000609663.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77111; AAC77111; b4151 BAE78155; BAE78155; BAE78155 |
GeneIDi | 58391487 948668 |
KEGGi | ecj:JW4112 eco:b4151 |
PATRICi | fig|1411691.4.peg.2547 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01611 Genomic DNA Translation: AAA23440.1 V00277 Genomic DNA Translation: CAA23536.1 U14003 Genomic DNA Translation: AAA97050.1 U00096 Genomic DNA Translation: AAC77111.1 AP009048 Genomic DNA Translation: BAE78155.1 M11979 Genomic DNA Translation: AAA23434.1 |
PIRi | A04431, WMEC13 |
RefSeqi | NP_418575.1, NC_000913.3 WP_000609663.1, NZ_STEB01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KF6 | X-ray | 2.70 | D/P | 1-119 | [»] | |
1KFY | X-ray | 3.60 | D/P | 1-119 | [»] | |
1L0V | X-ray | 3.30 | D/P | 1-119 | [»] | |
2B76 | X-ray | 3.30 | D/P | 1-119 | [»] | |
3CIR | X-ray | 3.65 | D/P | 1-119 | [»] | |
3P4P | X-ray | 2.80 | D/P | 1-119 | [»] | |
3P4Q | X-ray | 3.35 | D/P | 1-119 | [»] | |
3P4R | X-ray | 3.05 | D/P | 1-119 | [»] | |
3P4S | X-ray | 3.10 | D/P | 1-119 | [»] | |
4KX6 | X-ray | 2.95 | D/P | 1-119 | [»] | |
5VPN | X-ray | 4.22 | D/H | 1-119 | [»] | |
SMRi | P0A8Q3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262699, 457 interactors |
ComplexPortali | CPX-1967, Plasma membrane fumarate reductase complex |
DIPi | DIP-9684N |
IntActi | P0A8Q3, 1 interactor |
STRINGi | 511145.b4151 |
Chemistry databases
DrugBanki | DB07490, 2-[1-(4-CHLORO-PHENYL)-ETHYL]-4,6-DINITRO-PHENOL DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
Proteomic databases
jPOSTi | P0A8Q3 |
PaxDbi | P0A8Q3 |
PRIDEi | P0A8Q3 |
Genome annotation databases
EnsemblBacteriai | AAC77111; AAC77111; b4151 BAE78155; BAE78155; BAE78155 |
GeneIDi | 58391487 948668 |
KEGGi | ecj:JW4112 eco:b4151 |
PATRICi | fig|1411691.4.peg.2547 |
Organism-specific databases
EchoBASEi | EB0329 |
Phylogenomic databases
eggNOGi | COG3080, Bacteria |
HOGENOMi | CLU_168367_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:FUM-MEMB2 MetaCyc:FUM-MEMB2 |
Miscellaneous databases
EvolutionaryTracei | P0A8Q3 |
PROi | PR:P0A8Q3 |
Family and domain databases
CDDi | cd00547, QFR_TypeD_subunitD, 1 hit |
Gene3Di | 1.20.1300.10, 1 hit |
HAMAPi | MF_00709, Fumarate_red_D, 1 hit |
InterProi | View protein in InterPro IPR003418, Fumarate_red_D IPR034804, SQR/QFR_C/D |
Pfami | View protein in Pfam PF02313, Fumarate_red_D, 1 hit |
PIRSFi | PIRSF000179, FrdD, 1 hit |
SUPFAMi | SSF81343, SSF81343, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FRDD_ECOLI | |
Accessioni | P0A8Q3Primary (citable) accession number: P0A8Q3 Secondary accession number(s): P03806, Q2M6F1, Q47048 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families