UniProtKB - P0A8P1 (LFTR_ECOLI)
Protein
Leucyl/phenylalanyl-tRNA--protein transferase
Gene
aat
Organism
Escherichia coli (strain K12)
Status
Functioni
Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine.
Catalytic activityi
- L-leucyl-tRNALeu + N-terminal L-lysyl-[protein] = H+ + N-terminal L-leucyl-L-lysyl-[protein] + tRNALeuEC:2.3.2.6
- L-leucyl-tRNALeu + N-terminal L-arginyl-[protein] = H+ + N-terminal L-leucyl-L-arginyl-[protein] + tRNALeuEC:2.3.2.6
- an N-terminal L-α-aminoacyl-[protein] + L-phenylalanyl-tRNAPhe = an N-terminal L-phenylalanyl-L-α-aminoacyl-[protein] + tRNAPheEC:2.3.2.6
GO - Molecular functioni
- leucyltransferase activity Source: EcoCyc
- transferase activity, transferring amino-acyl groups Source: EcoliWiki
GO - Biological processi
- protein catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11112-MONOMER MetaCyc:EG11112-MONOMER |
BRENDAi | 2.3.2.6, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Leucyl/phenylalanyl-tRNA--protein transferase (EC:2.3.2.6)Alternative name(s): L/F-transferase Leucyltransferase Phenyalanyltransferase |
Gene namesi | Name:aat Synonyms:ycaA Ordered Locus Names:b0885, JW0868 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207217 | 1 – 234 | Leucyl/phenylalanyl-tRNA--protein transferaseAdd BLAST | 234 |
Proteomic databases
jPOSTi | P0A8P1 |
PaxDbi | P0A8P1 |
PRIDEi | P0A8P1 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4259988, 43 interactors 849864, 2 interactors |
DIPi | DIP-48235N |
IntActi | P0A8P1, 10 interactors |
STRINGi | 511145.b0885 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8P1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8P1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the L/F-transferase family.Curated
Phylogenomic databases
eggNOGi | COG2360, Bacteria |
InParanoidi | P0A8P1 |
PhylomeDBi | P0A8P1 |
Family and domain databases
Gene3Di | 3.40.630.70, 1 hit |
HAMAPi | MF_00688, Leu_Phe_trans, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR004616, Leu/Phe-tRNA_Trfase IPR042203, Leu/Phe-tRNA_Trfase_C |
PANTHERi | PTHR30098, PTHR30098, 1 hit |
Pfami | View protein in Pfam PF03588, Leu_Phe_trans, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR00667, aat, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8P1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF
60 70 80 90 100
SPGDPILWWS PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE
110 120 130 140 150
GCASDREEGT WITRGVVEAY HRLHELGHAH SIEVWREDEL VGGMYGVAQG
160 170 180 190 200
TLFCGESMFS RMENASKTAL LVFCEEFIGH GGKLIDCQVL NDHTASLGAC
210 220 230
EIPRRDYLNY LNQMRLGRLP NNFWVPRCLF SPQE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63145 Genomic DNA Translation: AAC36910.1 L10383 Unassigned DNA Translation: AAA03231.1 U00096 Genomic DNA Translation: AAC73971.1 AP009048 Genomic DNA Translation: BAA35605.1 |
PIRi | A36888 |
RefSeqi | NP_415405.1, NC_000913.3 WP_001241678.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC73971; AAC73971; b0885 BAA35605; BAA35605; BAA35605 |
GeneIDi | 58462755 945490 |
KEGGi | ecj:JW0868 eco:b0885 |
PATRICi | fig|1411691.4.peg.1393 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63145 Genomic DNA Translation: AAC36910.1 L10383 Unassigned DNA Translation: AAA03231.1 U00096 Genomic DNA Translation: AAC73971.1 AP009048 Genomic DNA Translation: BAA35605.1 |
PIRi | A36888 |
RefSeqi | NP_415405.1, NC_000913.3 WP_001241678.1, NZ_STEB01000006.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CXA | X-ray | 1.60 | A | 1-234 | [»] | |
2DPS | X-ray | 2.40 | A/B | 1-234 | [»] | |
2DPT | X-ray | 2.75 | A/B | 1-234 | [»] | |
2Z3K | X-ray | 2.85 | A/B | 2-234 | [»] | |
2Z3L | X-ray | 2.75 | A/B | 2-234 | [»] | |
2Z3M | X-ray | 2.70 | A/B | 2-234 | [»] | |
2Z3N | X-ray | 2.50 | A/B | 2-234 | [»] | |
2Z3O | X-ray | 2.40 | A/B | 2-234 | [»] | |
2Z3P | X-ray | 2.50 | A/B | 2-234 | [»] | |
SMRi | P0A8P1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259988, 43 interactors 849864, 2 interactors |
DIPi | DIP-48235N |
IntActi | P0A8P1, 10 interactors |
STRINGi | 511145.b0885 |
Chemistry databases
DrugBanki | DB08437, Puromycin |
Proteomic databases
jPOSTi | P0A8P1 |
PaxDbi | P0A8P1 |
PRIDEi | P0A8P1 |
Genome annotation databases
EnsemblBacteriai | AAC73971; AAC73971; b0885 BAA35605; BAA35605; BAA35605 |
GeneIDi | 58462755 945490 |
KEGGi | ecj:JW0868 eco:b0885 |
PATRICi | fig|1411691.4.peg.1393 |
Organism-specific databases
EchoBASEi | EB1103 |
Phylogenomic databases
eggNOGi | COG2360, Bacteria |
InParanoidi | P0A8P1 |
PhylomeDBi | P0A8P1 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11112-MONOMER MetaCyc:EG11112-MONOMER |
BRENDAi | 2.3.2.6, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A8P1 |
PROi | PR:P0A8P1 |
Family and domain databases
Gene3Di | 3.40.630.70, 1 hit |
HAMAPi | MF_00688, Leu_Phe_trans, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR004616, Leu/Phe-tRNA_Trfase IPR042203, Leu/Phe-tRNA_Trfase_C |
PANTHERi | PTHR30098, PTHR30098, 1 hit |
Pfami | View protein in Pfam PF03588, Leu_Phe_trans, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR00667, aat, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LFTR_ECOLI | |
Accessioni | P0A8P1Primary (citable) accession number: P0A8P1 Secondary accession number(s): P23885 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families