UniProtKB - P0A8N5 (SYK2_ECOLI)
Protein
Lysine--tRNA ligase, heat inducible
Gene
lysU
Organism
Escherichia coli (strain K12)
Status
Functioni
Also can synthesize a number of adenyl dinucleotides (in particular AppppA). These dinucleotides have been proposed to act as modulators of the heat-shock response and stress response.
Miscellaneous
There are two lysyl-tRNA ligases in E.coli: lysS is expressed constitutively, while lysU is heat inducible.
Catalytic activityi
- EC:6.1.1.6
Cofactori
Mg2+Note: Binds 3 Mg2+ ions per subunit. The third one is coordinated by ATP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 415 | Magnesium 1 | 1 | |
Metal bindingi | 422 | Magnesium 1 | 1 | |
Metal bindingi | 422 | Magnesium 2 | 1 |
GO - Molecular functioni
- ATP binding Source: EcoliWiki
- ligase activity Source: EcoliWiki
- lysine-tRNA ligase activity Source: EcoCyc
- magnesium ion binding Source: EcoliWiki
- tRNA binding Source: GO_Central
GO - Biological processi
- lysyl-tRNA aminoacylation Source: EcoCyc
- tRNA aminoacylation for protein translation Source: EcoliWiki
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSU-MONOMER MetaCyc:LYSU-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine--tRNA ligase, heat inducible (EC:6.1.1.6)Alternative name(s): Lysyl-tRNA synthetase Short name: LysRS |
Gene namesi | Name:lysU Ordered Locus Names:b4129, JW4090 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000152627 | 2 – 505 | Lysine--tRNA ligase, heat inducibleAdd BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 114 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 156 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0A8N5 |
PaxDbi | P0A8N5 |
PRIDEi | P0A8N5 |
PTM databases
iPTMneti | P0A8N5 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4262688, 18 interactors |
DIPi | DIP-36212N |
IntActi | P0A8N5, 52 interactors |
STRINGi | 511145.b4129 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8N5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8N5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
HOGENOMi | CLU_008255_6_0_6 |
InParanoidi | P0A8N5 |
PhylomeDBi | P0A8N5 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A8N5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEQETRGAN EAIDFNDELR NRREKLAALR QQGVAFPNDF RRDHTSDQLH
60 70 80 90 100
EEFDAKDNQE LESLNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA
110 120 130 140 150
RDSLPEGVYN DQFKKWDLGD IIGARGTLFK TQTGELSIHC TELRLLTKAL
160 170 180 190 200
RPLPDKFHGL QDQEVRYRQR YLDLIANDKS RQTFVVRSKI LAAIRQFMVA
210 220 230 240 250
RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG
260 270 280 290 300
GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYHDL IELTESLFRT
310 320 330 340 350
LAQEVLGTTK VTYGEHVFDF GKPFEKLTMR EAIKKYRPET DMADLDNFDA
360 370 380 390 400
AKALAESIGI TVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL
410 420 430 440 450
ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAERF QEQVNAKAAG
460 470 480 490 500
DDEAMFYDED YVTALEYGLP PTAGLGIGID RMIMLFTNSH TIRDVILFPA
MRPQK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 125 | Missing in AAA24096 (PubMed:2188953).Curated | 1 | |
Sequence conflicti | 236 | L → A in AAA24096 (PubMed:2188953).Curated | 1 | |
Sequence conflicti | 258 – 262 | INRNF → HVT in AAA24096 (PubMed:2188953).Curated | 5 | |
Sequence conflicti | 268 – 269 | SV → R in AAA24096 (PubMed:2188953).Curated | 2 | |
Sequence conflicti | 351 | A → R in AAA24096 (PubMed:2188953).Curated | 1 | |
Sequence conflicti | 371 | I → S in AAA24096 (PubMed:2188953).Curated | 1 | |
Sequence conflicti | 380 – 384 | AEAHL → VEGHV in AAA24096 (PubMed:2188953).Curated | 5 | |
Sequence conflicti | 388 | T → S in AAA24096 (PubMed:2188953).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16542 Genomic DNA Translation: CAA34542.1 M30630 Genomic DNA Translation: AAA24096.1 U14003 Genomic DNA Translation: AAA97029.1 U00096 Genomic DNA Translation: AAC77090.1 AP009048 Genomic DNA Translation: BAE78132.1 |
PIRi | S56358, SYECKU |
RefSeqi | NP_418553.1, NC_000913.3 WP_001295074.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77090; AAC77090; b4129 BAE78132; BAE78132; BAE78132 |
GeneIDi | 57730023 948645 |
KEGGi | ecj:JW4090 eco:b4129 |
PATRICi | fig|1411691.4.peg.2570 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16542 Genomic DNA Translation: CAA34542.1 M30630 Genomic DNA Translation: AAA24096.1 U14003 Genomic DNA Translation: AAA97029.1 U00096 Genomic DNA Translation: AAC77090.1 AP009048 Genomic DNA Translation: BAE78132.1 |
PIRi | S56358, SYECKU |
RefSeqi | NP_418553.1, NC_000913.3 WP_001295074.1, NZ_STEB01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E1O | X-ray | 2.12 | A | 2-505 | [»] | |
1E1T | X-ray | 2.40 | A | 2-505 | [»] | |
1E22 | X-ray | 2.43 | A | 2-505 | [»] | |
1E24 | X-ray | 2.35 | A | 2-505 | [»] | |
1LYL | X-ray | 2.80 | A/B/C | 2-505 | [»] | |
5YZX | X-ray | 3.20 | A/B/C | 1-505 | [»] | |
SMRi | P0A8N5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262688, 18 interactors |
DIPi | DIP-36212N |
IntActi | P0A8N5, 52 interactors |
STRINGi | 511145.b4129 |
PTM databases
iPTMneti | P0A8N5 |
Proteomic databases
jPOSTi | P0A8N5 |
PaxDbi | P0A8N5 |
PRIDEi | P0A8N5 |
Genome annotation databases
EnsemblBacteriai | AAC77090; AAC77090; b4129 BAE78132; BAE78132; BAE78132 |
GeneIDi | 57730023 948645 |
KEGGi | ecj:JW4090 eco:b4129 |
PATRICi | fig|1411691.4.peg.2570 |
Organism-specific databases
EchoBASEi | EB0548 |
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
HOGENOMi | CLU_008255_6_0_6 |
InParanoidi | P0A8N5 |
PhylomeDBi | P0A8N5 |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSU-MONOMER MetaCyc:LYSU-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A8N5 |
PROi | PR:P0A8N5 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYK2_ECOLI | |
Accessioni | P0A8N5Primary (citable) accession number: P0A8N5 Secondary accession number(s): P14825, Q2M6H4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families