UniProtKB - P0A8N3 (SYK1_ECOLI)
Protein
Lysine--tRNA ligase
Gene
lysS
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
There are two lysyl-tRNA ligases in E.coli: lysS is expressed constitutively, while lysU is heat inducible.
Catalytic activityi
- EC:6.1.1.6
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 415 | Magnesium 1By similarity | 1 | |
Metal bindingi | 422 | Magnesium 1By similarity | 1 | |
Metal bindingi | 422 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ligase activity Source: EcoliWiki
- lysine-tRNA ligase activity Source: EcoCyc
- magnesium ion binding Source: UniProtKB-UniRule
- tRNA binding Source: GO_Central
GO - Biological processi
- lysyl-tRNA aminoacylation Source: EcoliWiki
- tRNA aminoacylation for protein translation Source: EcoliWiki
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSS-MONOMER MetaCyc:LYSS-MONOMER |
BRENDAi | 6.1.1.6, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine--tRNA ligase (EC:6.1.1.6)Alternative name(s): Lysyl-tRNA synthetase Short name: LysRS |
Gene namesi | Name:lysS Synonyms:asuD, herC Ordered Locus Names:b2890, JW2858 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000152622 | 2 – 505 | Lysine--tRNA ligaseAdd BLAST | 504 |
Proteomic databases
jPOSTi | P0A8N3 |
PaxDbi | P0A8N3 |
PRIDEi | P0A8N3 |
2D gel databases
SWISS-2DPAGEi | P0A8N3 |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4262337, 591 interactors |
DIPi | DIP-36211N |
IntActi | P0A8N3, 25 interactors |
STRINGi | 511145.b2890 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0A8N3 |
SMRi | P0A8N3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8N3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
HOGENOMi | CLU_008255_6_0_6 |
InParanoidi | P0A8N3 |
PhylomeDBi | P0A8N3 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A8N3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEQHAQGAD AVVDLNNELK TRREKLANLR EQGIAFPNDF RRDHTSDQLH
60 70 80 90 100
AEFDGKENEE LEALNIEVAV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA
110 120 130 140 150
RDDLPEGVYN EQFKKWDLGD ILGAKGKLFK TKTGELSIHC TELRLLTKAL
160 170 180 190 200
RPLPDKFHGL QDQEARYRQR YLDLISNDES RNTFKVRSQI LSGIRQFMVN
210 220 230 240 250
RGFMEVETPM MQVIPGGAAA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG
260 270 280 290 300
GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT
310 320 330 340 350
LAQDILGKTE VTYGDVTLDF GKPFEKLTMR EAIKKYRPET DMADLDNFDS
360 370 380 390 400
AKAIAESIGI HVEKSWGLGR IVTEIFEEVA EAHLIQPTFI TEYPAEVSPL
410 420 430 440 450
ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAQRF LDQVAAKDAG
460 470 480 490 500
DDEAMFYDED YVTALEHGLP PTAGLGIGID RMVMLFTNSH TIRDVILFPA
MRPVK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03795 Genomic DNA Translation: AAA23959.1 U28375 Genomic DNA Translation: AAA83071.1 U00096 Genomic DNA Translation: AAC75928.1 AP009048 Genomic DNA Translation: BAE76955.1 |
PIRi | B65073, SYECKT |
RefSeqi | NP_417366.1, NC_000913.3 WP_000003071.1, NZ_SSZK01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75928; AAC75928; b2890 BAE76955; BAE76955; BAE76955 |
GeneIDi | 57730301 947372 |
KEGGi | ecj:JW2858 eco:b2890 |
PATRICi | fig|1411691.4.peg.3844 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03795 Genomic DNA Translation: AAA23959.1 U28375 Genomic DNA Translation: AAA83071.1 U00096 Genomic DNA Translation: AAC75928.1 AP009048 Genomic DNA Translation: BAE76955.1 |
PIRi | B65073, SYECKT |
RefSeqi | NP_417366.1, NC_000913.3 WP_000003071.1, NZ_SSZK01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BBU | X-ray | 2.70 | A | 2-505 | [»] | |
1BBW | X-ray | 2.70 | A | 2-505 | [»] | |
1KRS | NMR | - | A | 31-149 | [»] | |
1KRT | NMR | - | A | 31-149 | [»] | |
BMRBi | P0A8N3 | |||||
SMRi | P0A8N3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262337, 591 interactors |
DIPi | DIP-36211N |
IntActi | P0A8N3, 25 interactors |
STRINGi | 511145.b2890 |
2D gel databases
SWISS-2DPAGEi | P0A8N3 |
Proteomic databases
jPOSTi | P0A8N3 |
PaxDbi | P0A8N3 |
PRIDEi | P0A8N3 |
Genome annotation databases
EnsemblBacteriai | AAC75928; AAC75928; b2890 BAE76955; BAE76955; BAE76955 |
GeneIDi | 57730301 947372 |
KEGGi | ecj:JW2858 eco:b2890 |
PATRICi | fig|1411691.4.peg.3844 |
Organism-specific databases
EchoBASEi | EB0547 |
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
HOGENOMi | CLU_008255_6_0_6 |
InParanoidi | P0A8N3 |
PhylomeDBi | P0A8N3 |
Enzyme and pathway databases
BioCyci | EcoCyc:LYSS-MONOMER MetaCyc:LYSS-MONOMER |
BRENDAi | 6.1.1.6, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A8N3 |
PROi | PR:P0A8N3 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYK1_ECOLI | |
Accessioni | P0A8N3Primary (citable) accession number: P0A8N3 Secondary accession number(s): P13030, Q2M9V1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families