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Protein

ATP-dependent RNA helicase RhlB

Gene

rhlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DEAD-box RNA helicase involved in RNA degradation. Has RNA-dependent ATPase activity and unwinds double-stranded RNA.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation2 Publications

Activity regulationi

ATPase activity is stimulated by interaction with RNase E.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 60ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-UniRule
  • ATP-dependent RNA helicase activity Source: CAFA
  • disordered domain specific binding Source: CAFA
  • RNA binding Source: UniProtKB-UniRule

GO - Biological processi

  • RNA catabolic process Source: EcoCyc
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10844-MONOMER
MetaCyc:EG10844-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase RhlBUniRule annotation (EC:3.6.4.13UniRule annotation)
Gene namesi
Name:rhlBUniRule annotation
Synonyms:mmrA
Ordered Locus Names:b3780, JW3753
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10844 rhlB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002007692 – 421ATP-dependent RNA helicase RhlBAdd BLAST420

Proteomic databases

EPDiP0A8J8
PaxDbiP0A8J8
PRIDEiP0A8J8

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Binds to RNase E (rne) and PNPase (pnp). Forms multimers.UniRule annotation4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263320, 99 interactors
ComplexPortaliCPX-403 Degradosome
DIPiDIP-35644N
IntActiP0A8J8, 33 interactors
MINTiP0A8J8
STRINGi316385.ECDH10B_3969

Structurei

3D structure databases

ProteinModelPortaliP0A8J8
SMRiP0A8J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 219Helicase ATP-bindingUniRule annotationAdd BLAST180
Domaini245 – 390Helicase C-terminalUniRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 37Q motifAdd BLAST29
Motifi165 – 168DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family. RhlB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1J Bacteria
COG0513 LUCA
HOGENOMiHOG000268807
InParanoidiP0A8J8
KOiK03732
OMAiRNPIRIL
PhylomeDBiP0A8J8

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00661 DEAD_helicase_RhlB, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR023554 RNA_helicase_ATP-dep_RhlB
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8J8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA
60 70 80 90 100
GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI
110 120 130 140 150
HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK
160 170 180 190 200
QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT
210 220 230 240 250
LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL
260 270 280 290 300
IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD
310 320 330 340 350
EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA
360 370 380 390 400
GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT
410 420
RPRTGNGPRR TGAPRNRRRS G
Length:421
Mass (Da):47,126
Last modified:January 23, 2007 - v2
Checksum:i48E1ADD025CBFA1A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72L → H in X56310 (PubMed:1931833).Curated1
Sequence conflicti340Y → S in X56310 (PubMed:1931833).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56310 Genomic DNA No translation available.
M87049 Genomic DNA Translation: AAA67581.1
M83316 Genomic DNA Translation: AAB59048.1
U00096 Genomic DNA Translation: AAC76785.1
AP009048 Genomic DNA Translation: BAE77518.1
PIRiG65181
RefSeqiNP_418227.1, NC_000913.3
WP_000047499.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76785; AAC76785; b3780
BAE77518; BAE77518; BAE77518
GeneIDi948290
KEGGiecj:JW3753
eco:b3780
PATRICifig|511145.12.peg.3895

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56310 Genomic DNA No translation available.
M87049 Genomic DNA Translation: AAA67581.1
M83316 Genomic DNA Translation: AAB59048.1
U00096 Genomic DNA Translation: AAC76785.1
AP009048 Genomic DNA Translation: BAE77518.1
PIRiG65181
RefSeqiNP_418227.1, NC_000913.3
WP_000047499.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP0A8J8
SMRiP0A8J8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263320, 99 interactors
ComplexPortaliCPX-403 Degradosome
DIPiDIP-35644N
IntActiP0A8J8, 33 interactors
MINTiP0A8J8
STRINGi316385.ECDH10B_3969

Proteomic databases

EPDiP0A8J8
PaxDbiP0A8J8
PRIDEiP0A8J8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76785; AAC76785; b3780
BAE77518; BAE77518; BAE77518
GeneIDi948290
KEGGiecj:JW3753
eco:b3780
PATRICifig|511145.12.peg.3895

Organism-specific databases

EchoBASEiEB0837
EcoGeneiEG10844 rhlB

Phylogenomic databases

eggNOGiENOG4105C1J Bacteria
COG0513 LUCA
HOGENOMiHOG000268807
InParanoidiP0A8J8
KOiK03732
OMAiRNPIRIL
PhylomeDBiP0A8J8

Enzyme and pathway databases

BioCyciEcoCyc:EG10844-MONOMER
MetaCyc:EG10844-MONOMER

Miscellaneous databases

PROiPR:P0A8J8

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00661 DEAD_helicase_RhlB, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR023554 RNA_helicase_ATP-dep_RhlB
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHLB_ECOLI
AccessioniPrimary (citable) accession number: P0A8J8
Secondary accession number(s): P24229, Q2M888
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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