UniProtKB - P0A8I8 (RLMH_ECOLI)
Protein
Ribosomal RNA large subunit methyltransferase H
Gene
rlmH
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes.4 Publications
Catalytic activityi
- pseudouridine1915 in 23S rRNA + S-adenosyl-L-methionine = H+ + N3-methylpseudouridine1915 in 23S rRNA + S-adenosyl-L-homocysteineUniRule annotation4 PublicationsEC:2.1.1.177UniRule annotation4 Publications
Kineticsi
kcat is 5-6 min(-1) (PubMed:20817755). kcat is 2.5 min(-1) (PubMed:28428565).2 Publications
- KM=0.51 µM for 70S ribosome1 Publication
- KM=0.3 µM for 70S ribosome1 Publication
- KM=27 µM for S-adenosyl-L-methionine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 72 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 103 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotationCombined sources1 Publication | 1 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- ribosome binding Source: UniProtKB
- rRNA (pseudouridine-N3-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- rRNA base methylation Source: EcoCyc
- rRNA methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11254-MONOMER MetaCyc:EG11254-MONOMER |
BRENDAi | 2.1.1.177, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA large subunit methyltransferase HUniRule annotationCurated (EC:2.1.1.177UniRule annotation4 Publications)Alternative name(s): 23S rRNA (pseudouridine1915-N3)-methyltransferaseUniRule annotationCurated 23S rRNA m3Psi1915 methyltransferaseUniRule annotationCurated rRNA (pseudouridine-N3-)-methyltransferase RlmHUniRule annotationCurated |
Gene namesi | Name:rlmH2 PublicationsUniRule annotation Synonyms:ybeA Ordered Locus Names:b0636, JW0631 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotationCurated
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 129 | H → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 138 | E → A or Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 142 | R → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 152 | Y → F: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 153 | H → F: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 154 | R → A: Loss of activity. Impairs ribosome binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198115 | 1 – 155 | Ribosomal RNA large subunit methyltransferase HAdd BLAST | 155 |
Proteomic databases
jPOSTi | P0A8I8 |
PaxDbi | P0A8I8 |
PRIDEi | P0A8I8 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsBinary interactionsi
P0A8I8
With | #Exp. | IntAct |
---|---|---|
rpsB [P0A7V0] | 2 | EBI-560148,EBI-543439 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 4259479, 46 interactors |
DIPi | DIP-11357N |
IntActi | P0A8I8, 11 interactors |
STRINGi | 511145.b0636 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8I8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8I8 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 122 – 127 | S-adenosyl-L-methionine bindingUniRule annotationCombined sources1 Publication | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1576, Bacteria |
HOGENOMi | CLU_100552_1_0_6 |
InParanoidi | P0A8I8 |
PhylomeDBi | P0A8I8 |
Family and domain databases
CDDi | cd18081, RlmH-like, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_00658, 23SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR003742, RlmH-like IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR33603, PTHR33603, 1 hit |
Pfami | View protein in Pfam PF02590, SPOUT_MTase, 1 hit |
PIRSFi | PIRSF004505, MT_bac, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00246, tRNA_RlmH_YbeA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8I8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK
60 70 80 90 100
RILDKEGEQM LAAAGKNRIV TLDIPGKPWD TPQLAAELER WKLDGRDVSL
110 120 130 140 150
LIGGPEGLSP ACKAAAEQSW SLSALTLPHP LVRVLVAESL YRAWSITTNH
PYHRE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04516 Genomic DNA Translation: CAA28200.1 U82598 Genomic DNA Translation: AAB40836.1 U00096 Genomic DNA Translation: AAC73737.1 AP009048 Genomic DNA Translation: BAA35283.1 |
PIRi | B24995, QQECP1 |
RefSeqi | NP_415169.1, NC_000913.3 WP_000776104.1, NZ_STEB01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC73737; AAC73737; b0636 BAA35283; BAA35283; BAA35283 |
GeneIDi | 57728814 945239 |
KEGGi | ecj:JW0631 eco:b0636 |
PATRICi | fig|1411691.4.peg.1632 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04516 Genomic DNA Translation: CAA28200.1 U82598 Genomic DNA Translation: AAB40836.1 U00096 Genomic DNA Translation: AAC73737.1 AP009048 Genomic DNA Translation: BAA35283.1 |
PIRi | B24995, QQECP1 |
RefSeqi | NP_415169.1, NC_000913.3 WP_000776104.1, NZ_STEB01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NS5 | X-ray | 1.68 | A/B | 1-155 | [»] | |
5TWJ | X-ray | 2.30 | A/B/C/D | 2-155 | [»] | |
5TWK | X-ray | 2.10 | A/B/C/D | 2-155 | [»] | |
5ZYO | X-ray | 1.75 | A/B/C/D | 1-155 | [»] | |
SMRi | P0A8I8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259479, 46 interactors |
DIPi | DIP-11357N |
IntActi | P0A8I8, 11 interactors |
STRINGi | 511145.b0636 |
Proteomic databases
jPOSTi | P0A8I8 |
PaxDbi | P0A8I8 |
PRIDEi | P0A8I8 |
Genome annotation databases
EnsemblBacteriai | AAC73737; AAC73737; b0636 BAA35283; BAA35283; BAA35283 |
GeneIDi | 57728814 945239 |
KEGGi | ecj:JW0631 eco:b0636 |
PATRICi | fig|1411691.4.peg.1632 |
Organism-specific databases
EchoBASEi | EB1234 |
Phylogenomic databases
eggNOGi | COG1576, Bacteria |
HOGENOMi | CLU_100552_1_0_6 |
InParanoidi | P0A8I8 |
PhylomeDBi | P0A8I8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11254-MONOMER MetaCyc:EG11254-MONOMER |
BRENDAi | 2.1.1.177, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A8I8 |
PROi | PR:P0A8I8 |
Family and domain databases
CDDi | cd18081, RlmH-like, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_00658, 23SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR003742, RlmH-like IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR33603, PTHR33603, 1 hit |
Pfami | View protein in Pfam PF02590, SPOUT_MTase, 1 hit |
PIRSFi | PIRSF004505, MT_bac, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00246, tRNA_RlmH_YbeA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLMH_ECOLI | |
Accessioni | P0A8I8Primary (citable) accession number: P0A8I8 Secondary accession number(s): P05850 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families