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Protein

UvrABC system protein B

Gene

uvrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.1 Publication

Miscellaneous

According to PubMed:3515321, a cleaved form of the protein was observed that resulted from the removal of about 40 amino acids from the C-terminus of the protein. The exact cleavage site being unknown, it was proposed to be between Lys-630 and Ala-631. There was no indication that cleavage occured in vivo and therefore it is not known if it has any physiological significance.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-UniRule
  • DNA binding Source: UniProtKB-UniRule
  • excinuclease ABC activity Source: UniProtKB-UniRule
  • identical protein binding Source: IntAct

GO - Biological processi

  • nucleotide-excision repair Source: UniProtKB-UniRule
  • response to radiation Source: EcoCyc
  • SOS response Source: UniProtKB-UniRule

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11062-MONOMER
MetaCyc:EG11062-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
Ordered Locus Names:b0779, JW0762
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11062 uvrB

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • excinuclease repair complex Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95 – 96YY → AA: Defective in DNA-unwinding activity. 1 Publication2
Mutagenesisi101Y → A: Defective in DNA-unwinding activity; when associated with A-108. 1 Publication1
Mutagenesisi108F → A: Defective in DNA-unwinding activity; when associated with A-101. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001383902 – 673UvrABC system protein BAdd BLAST672

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei630 – 631CleavageSequence analysis2

Proteomic databases

PaxDbiP0A8F8
PRIDEiP0A8F8

Expressioni

Inductioni

Induced 1.5-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259957, 140 interactors
ComplexPortaliCPX-2151 UvrAB complex
CPX-2152 UvrB complex
CPX-2153 UvrBC complex
DIPiDIP-48012N
IntActiP0A8F8, 32 interactors
STRINGi316385.ECDH10B_0847

Structurei

Secondary structure

1673
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A8F8
SMRiP0A8F8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8F8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 415Helicase ATP-bindingAdd BLAST390
Domaini431 – 597Helicase C-terminalAdd BLAST167
Domaini633 – 668UVRAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.

Sequence similaritiesi

Belongs to the UvrB family.Curated

Phylogenomic databases

eggNOGiENOG4105CCW Bacteria
COG0556 LUCA
HOGENOMiHOG000073580
InParanoidiP0A8F8
KOiK03702
PhylomeDBiP0A8F8

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00204 UvrB, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
IPR036876 UVR_dom_sf
IPR004807 UvrB
IPR024759 UvrB_YAD/RRR_dom
PANTHERiPTHR24029 PTHR24029, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF02151 UVR, 1 hit
PF12344 UvrB, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF46600 SSF46600, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00631 uvrb, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50151 UVR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8F8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA
60 70 80 90 100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD
160 170 180 190 200
PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI
210 220 230 240 250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV
260 270 280 290 300
TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL
310 320 330 340 350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM
360 370 380 390 400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE
410 420 430 440 450
LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL
460 470 480 490 500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL
510 520 530 540 550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA
560 570 580 590 600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
610 620 630 640 650
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL
660 670
EFEEAAQIRD QLHQLRELFI AAS
Length:673
Mass (Da):76,226
Last modified:January 23, 2007 - v2
Checksum:i2F172045344FDAD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti477H → R in CAA27314 (PubMed:3515321).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03678 Genomic DNA Translation: CAA27314.1
X03722 Genomic DNA Translation: CAA27357.1
U00096 Genomic DNA Translation: AAC73866.1
AP009048 Genomic DNA Translation: BAA35437.2
PIRiA93613 BVECUB
RefSeqiNP_415300.1, NC_000913.3
WP_000042533.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73866; AAC73866; b0779
BAA35437; BAA35437; BAA35437
GeneIDi945385
KEGGiecj:JW0762
eco:b0779
PATRICifig|1411691.4.peg.1499

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03678 Genomic DNA Translation: CAA27314.1
X03722 Genomic DNA Translation: CAA27357.1
U00096 Genomic DNA Translation: AAC73866.1
AP009048 Genomic DNA Translation: BAA35437.2
PIRiA93613 BVECUB
RefSeqiNP_415300.1, NC_000913.3
WP_000042533.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E52NMR-A/B619-673[»]
1QOJX-ray3.00A/B619-673[»]
ProteinModelPortaliP0A8F8
SMRiP0A8F8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259957, 140 interactors
ComplexPortaliCPX-2151 UvrAB complex
CPX-2152 UvrB complex
CPX-2153 UvrBC complex
DIPiDIP-48012N
IntActiP0A8F8, 32 interactors
STRINGi316385.ECDH10B_0847

Proteomic databases

PaxDbiP0A8F8
PRIDEiP0A8F8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73866; AAC73866; b0779
BAA35437; BAA35437; BAA35437
GeneIDi945385
KEGGiecj:JW0762
eco:b0779
PATRICifig|1411691.4.peg.1499

Organism-specific databases

EchoBASEiEB1055
EcoGeneiEG11062 uvrB

Phylogenomic databases

eggNOGiENOG4105CCW Bacteria
COG0556 LUCA
HOGENOMiHOG000073580
InParanoidiP0A8F8
KOiK03702
PhylomeDBiP0A8F8

Enzyme and pathway databases

BioCyciEcoCyc:EG11062-MONOMER
MetaCyc:EG11062-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A8F8
PROiPR:P0A8F8

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00204 UvrB, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
IPR036876 UVR_dom_sf
IPR004807 UvrB
IPR024759 UvrB_YAD/RRR_dom
PANTHERiPTHR24029 PTHR24029, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF02151 UVR, 1 hit
PF12344 UvrB, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF46600 SSF46600, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00631 uvrb, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50151 UVR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_ECOLI
AccessioniPrimary (citable) accession number: P0A8F8
Secondary accession number(s): P07025
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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