UniProtKB - P0A8F8 (UVRB_ECOLI)
Protein
UvrABC system protein B
Gene
uvrB
Organism
Escherichia coli (strain K12)
Status
Functioni
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.1 Publication
Miscellaneous
According to PubMed:3515321, a cleaved form of the protein was observed that resulted from the removal of about 40 amino acids from the C-terminus of the protein. The exact cleavage site being unknown, it was proposed to be between Lys-630 and Ala-631. There was no indication that cleavage occured in vivo and therefore it is not known if it has any physiological significance.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 46 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- excinuclease ABC activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
GO - Biological processi
- nucleotide-excision repair Source: UniProtKB-UniRule
- response to radiation Source: EcoCyc
- SOS response Source: UniProtKB-UniRule
Keywordsi
Molecular function | Excision nuclease |
Biological process | DNA damage, DNA excision, DNA repair, SOS response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11062-MONOMER MetaCyc:EG11062-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: UvrABC system protein BShort name: Protein UvrB Alternative name(s): Excinuclease ABC subunit B |
Gene namesi | Name:uvrB Ordered Locus Names:b0779, JW0762 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
- excinuclease repair complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 95 – 96 | YY → AA: Defective in DNA-unwinding activity. 1 Publication | 2 | |
Mutagenesisi | 101 | Y → A: Defective in DNA-unwinding activity; when associated with A-108. 1 Publication | 1 | |
Mutagenesisi | 108 | F → A: Defective in DNA-unwinding activity; when associated with A-101. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000138390 | 2 – 673 | UvrABC system protein BAdd BLAST | 672 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 630 – 631 | CleavageSequence analysis | 2 |
Proteomic databases
jPOSTi | P0A8F8 |
PaxDbi | P0A8F8 |
PRIDEi | P0A8F8 |
Expressioni
Inductioni
Induced 1.5-fold by hydroxyurea.1 Publication
Interactioni
Subunit structurei
Forms a heterotetramer with UvrA during the search for lesions.
Interacts with UvrC in an incision complex.
Binary interactionsi
Hide detailsP0A8F8
With | #Exp. | IntAct |
---|---|---|
seqA [P0AFY8] | 2 | EBI-552176,EBI-552553 |
ugd [P76373] | 2 | EBI-552176,EBI-1120497 |
uvrA [P0A698] | 7 | EBI-552176,EBI-552091 |
itself | 3 | EBI-552176,EBI-552176 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259957, 140 interactors 849761, 19 interactors |
ComplexPortali | CPX-2151, UvrAB complex CPX-2152, UvrB complex CPX-2153, UvrBC complex |
DIPi | DIP-48012N |
IntActi | P0A8F8, 32 interactors |
STRINGi | 511145.b0779 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A8F8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8F8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 415 | Helicase ATP-bindingAdd BLAST | 390 | |
Domaini | 431 – 597 | Helicase C-terminalAdd BLAST | 167 | |
Domaini | 633 – 668 | UVRAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 92 – 115 | Beta-hairpinAdd BLAST | 24 |
Domaini
The beta-hairpin motif is involved in DNA binding.
Sequence similaritiesi
Belongs to the UvrB family.Curated
Phylogenomic databases
eggNOGi | COG0556, Bacteria |
HOGENOMi | CLU_009621_2_1_6 |
InParanoidi | P0A8F8 |
PhylomeDBi | P0A8F8 |
Family and domain databases
HAMAPi | MF_00204, UvrB, 1 hit |
InterProi | View protein in InterPro IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR001943, UVR_dom IPR036876, UVR_dom_sf IPR004807, UvrB IPR041471, UvrB_inter IPR024759, UvrB_YAD/RRR_dom |
PANTHERi | PTHR24029, PTHR24029, 1 hit |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF02151, UVR, 1 hit PF12344, UvrB, 1 hit PF17757, UvrB_inter, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF46600, SSF46600, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00631, uvrb, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50151, UVR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A8F8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA
60 70 80 90 100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD
160 170 180 190 200
PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI
210 220 230 240 250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV
260 270 280 290 300
TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL
310 320 330 340 350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM
360 370 380 390 400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE
410 420 430 440 450
LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL
460 470 480 490 500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL
510 520 530 540 550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA
560 570 580 590 600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
610 620 630 640 650
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL
660 670
EFEEAAQIRD QLHQLRELFI AAS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 477 | H → R in CAA27314 (PubMed:3515321).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03678 Genomic DNA Translation: CAA27314.1 X03722 Genomic DNA Translation: CAA27357.1 U00096 Genomic DNA Translation: AAC73866.1 AP009048 Genomic DNA Translation: BAA35437.2 |
PIRi | A93613, BVECUB |
RefSeqi | NP_415300.1, NC_000913.3 WP_000042533.1, NZ_STEB01000028.1 |
Genome annotation databases
EnsemblBacteriai | AAC73866; AAC73866; b0779 BAA35437; BAA35437; BAA35437 |
GeneIDi | 57728426 945385 |
KEGGi | ecj:JW0762 eco:b0779 |
PATRICi | fig|1411691.4.peg.1499 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03678 Genomic DNA Translation: CAA27314.1 X03722 Genomic DNA Translation: CAA27357.1 U00096 Genomic DNA Translation: AAC73866.1 AP009048 Genomic DNA Translation: BAA35437.2 |
PIRi | A93613, BVECUB |
RefSeqi | NP_415300.1, NC_000913.3 WP_000042533.1, NZ_STEB01000028.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E52 | NMR | - | A/B | 619-673 | [»] | |
1QOJ | X-ray | 3.00 | A/B | 619-673 | [»] | |
SMRi | P0A8F8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259957, 140 interactors 849761, 19 interactors |
ComplexPortali | CPX-2151, UvrAB complex CPX-2152, UvrB complex CPX-2153, UvrBC complex |
DIPi | DIP-48012N |
IntActi | P0A8F8, 32 interactors |
STRINGi | 511145.b0779 |
Proteomic databases
jPOSTi | P0A8F8 |
PaxDbi | P0A8F8 |
PRIDEi | P0A8F8 |
Genome annotation databases
EnsemblBacteriai | AAC73866; AAC73866; b0779 BAA35437; BAA35437; BAA35437 |
GeneIDi | 57728426 945385 |
KEGGi | ecj:JW0762 eco:b0779 |
PATRICi | fig|1411691.4.peg.1499 |
Organism-specific databases
EchoBASEi | EB1055 |
Phylogenomic databases
eggNOGi | COG0556, Bacteria |
HOGENOMi | CLU_009621_2_1_6 |
InParanoidi | P0A8F8 |
PhylomeDBi | P0A8F8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11062-MONOMER MetaCyc:EG11062-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A8F8 |
PROi | PR:P0A8F8 |
Family and domain databases
HAMAPi | MF_00204, UvrB, 1 hit |
InterProi | View protein in InterPro IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR001943, UVR_dom IPR036876, UVR_dom_sf IPR004807, UvrB IPR041471, UvrB_inter IPR024759, UvrB_YAD/RRR_dom |
PANTHERi | PTHR24029, PTHR24029, 1 hit |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF02151, UVR, 1 hit PF12344, UvrB, 1 hit PF17757, UvrB_inter, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF46600, SSF46600, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00631, uvrb, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50151, UVR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UVRB_ECOLI | |
Accessioni | P0A8F8Primary (citable) accession number: P0A8F8 Secondary accession number(s): P07025 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families