UniProtKB - P0A8F8 (UVRB_ECOLI)

BLAST

>sp|P0A8F8|UVRB_ECOLI UvrABC system protein B OS=Escherichia coli (strain K12) OX=83333 GN=uvrB PE=1 SV=2
MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSGKTFTIANVIADLQRPT
MVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIE
QMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLHLTVGMIIDQRAILRRLAELQ
YARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFDEEVERLSLFDPLTGQIVSTIPR
FTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLENNKLLEEQRLTQRTQFDLEMMNEL
GYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGLLVVDESHVTIPQIGGMYRGDRARKET
LVEYGFRLPSALDNRPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGLLD
PIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYLHSDI
DTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIG
RAARNVNGKAILYGDKITPSMAKAIGETERRREKQQKYNEEHGITPQGLNKKVVDILALG
QNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRD
QLHQLRELFIAAS

Align
Format
Add to basket Added to basket
History
Entry version 119 (10 Oct 2018)
Sequence version 2 (23 Jan 2007)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

UvrABC system protein B

Gene

uvrB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA₂B₂ complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.1 Publication

Miscellaneous

According to PubMed:3515321, a cleaved form of the protein was observed that resulted from the removal of about 40 amino acids from the C-terminus of the protein. The exact cleavage site being unknown, it was proposed to be between Lys-630 and Ala-631. There was no indication that cleavage occured in vivo and therefore it is not known if it has any physiological significance.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	39 – 46	ATPSequence analysis		8

GO - Molecular functionⁱ

ATPase activity Source: InterPro
ATP binding Source: UniProtKB-UniRule
DNA binding Source: UniProtKB-UniRule
excinuclease ABC activity Source: UniProtKB-UniRule
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 12145219
- 24561554

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

nucleotide-excision repair Source: UniProtKB-UniRule
response to radiation
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 27718375
SOS response Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Excision nuclease
Biological process	DNA damage, DNA excision, DNA repair, SOS response
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11062-MONOMER MetaCyc:EG11062-MONOMER

BioCycⁱ

EcoCyc:EG11062-MONOMER
MetaCyc:EG11062-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: UvrABC system protein B Short name: Protein UvrB Alternative name(s): Excinuclease ABC subunit B
Gene namesⁱ	Name:uvrB Ordered Locus Names:b0779, JW0762
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11062 uvrB

EcoGeneⁱ

EG11062 uvrB

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell
excinuclease repair complex
Source: UniProtKB
Inferred from direct assayⁱ
- 12145219

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	95 – 96	YY → AA: Defective in DNA-unwinding activity. 1 Publication	2
Mutagenesisⁱ	101	Y → A: Defective in DNA-unwinding activity; when associated with A-108. 1 Publication	1
Mutagenesisⁱ	108	F → A: Defective in DNA-unwinding activity; when associated with A-101. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000138390	2 – 673	UvrABC system protein BAdd BLAST		672

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	630 – 631	CleavageSequence analysis		2

Proteomic databases

PaxDbⁱ	P0A8F8
PRIDEⁱ	P0A8F8

Expressionⁱ

Inductionⁱ

Induced 1.5-fold by hydroxyurea.1 Publication

Interactionⁱ

Subunit structureⁱ

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		3	EBI-552176,EBI-552176
seqA	P0AFY8	2	EBI-552176,EBI-552553
ugd	P76373	2	EBI-552176,EBI-1120497
uvrA	P0A698	7	EBI-552176,EBI-552091

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 12145219
- 24561554

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4259957, 140 interactors
ComplexPortalⁱ	CPX-2151 UvrAB complex CPX-2152 UvrB complex CPX-2153 UvrBC complex
Database of interacting proteins More...DIPⁱ	DIP-48012N
IntActⁱ	P0A8F8, 32 interactors
STRINGⁱ	316385.ECDH10B_0847

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Helixⁱ	629 – 648	Combined sources		20
Helixⁱ	654 – 671	Combined sources		18

Show more details

3D structure databases

ProteinModelPortalⁱ	P0A8F8
SMRⁱ	P0A8F8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0A8F8

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	26 – 415	Helicase ATP-bindingAdd BLAST	390
Domainⁱ	431 – 597	Helicase C-terminalAdd BLAST	167
Domainⁱ	633 – 668	UVRAdd BLAST	36

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	92 – 115	Beta-hairpinAdd BLAST		24

Domainⁱ

The beta-hairpin motif is involved in DNA binding.

Sequence similaritiesⁱ

Belongs to the UvrB family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105CCW Bacteria COG0556 LUCA
HOGENOMⁱ	HOG000073580
InParanoidⁱ	P0A8F8
KEGG Orthology (KO) More...KOⁱ	K03702
OMAⁱ	RYMHSEI
PhylomeDBⁱ	P0A8F8

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00079 HELICc, 1 hit
HAMAPⁱ	MF_00204 UvrB, 1 hit
InterProⁱ	View protein in InterPro IPR006935 Helicase/UvrB_N IPR014001 Helicase_ATP-bd IPR001650 Helicase_C IPR027417 P-loop_NTPase IPR001943 UVR_dom IPR036876 UVR_dom_sf IPR004807 UvrB IPR024759 UvrB_YAD/RRR_dom
PANTHERⁱ	PTHR24029 PTHR24029, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00271 Helicase_C, 1 hit PF04851 ResIII, 1 hit PF02151 UVR, 1 hit PF12344 UvrB, 1 hit
SMARTⁱ	View protein in SMART SM00487 DEXDc, 1 hit SM00490 HELICc, 1 hit
SUPFAMⁱ	SSF46600 SSF46600, 1 hit SSF52540 SSF52540, 3 hits
TIGRFAMsⁱ	TIGR00631 uvrb, 1 hit
PROSITEⁱ	View protein in PROSITE PS51192 HELICASE_ATP_BIND_1, 1 hit PS51194 HELICASE_CTER, 1 hit PS50151 UVR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0A8F8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA 
        60         70         80         90        100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA 
       110        120        130        140        150
YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD 
       160        170        180        190        200
PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI 
       210        220        230        240        250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV 
       260        270        280        290        300
TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL 
       310        320        330        340        350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM 
       360        370        380        390        400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE 
       410        420        430        440        450
LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL 
       460        470        480        490        500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL 
       510        520        530        540        550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA 
       560        570        580        590        600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG 
       610        620        630        640        650
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL 
       660        670 
EFEEAAQIRD QLHQLRELFI AAS

Length:673

Mass (Da):76,226

Last modified:January 23, 2007 - v2

Checksum:ⁱ2F172045344FDAD7

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	477	H → R in CAA27314 (PubMed:3515321).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X03678 Genomic DNA Translation: CAA27314.1 X03722 Genomic DNA Translation: CAA27357.1 U00096 Genomic DNA Translation: AAC73866.1 AP009048 Genomic DNA Translation: BAA35437.2
PIRⁱ	A93613 BVECUB
NCBI Reference Sequences More...RefSeqⁱ	NP_415300.1, NC_000913.3 WP_000042533.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73866; AAC73866; b0779 BAA35437; BAA35437; BAA35437
GeneIDⁱ	945385
KEGGⁱ	ecj:JW0762 eco:b0779
PATRICⁱ	fig\|1411691.4.peg.1499

Protein	Similar proteins		Species	Score	Length	Source
P0A8F8	UvrABC system protein B		ECO24		673	UniRef100_A7ZJI8
UvrABC system protein B		ECO27		673
UvrABC system protein B		ECO45		673
UvrABC system protein B		ECO55		673
UvrABC system protein B		ECO57		673
+170

Protein	Similar proteins		Species	Score	Length	Source
P0A8F8	UvrABC system protein B		ECOL6		673	UniRef90_Q8FJP8
UvrABC system protein B		SHIF8		673
UvrABC system protein B		SHIFL		673
UvrABC system protein B		SHIDS		673
UvrABC system protein B		SALTI		673
+1343

Protein	Similar proteins		Species	Score	Length	Source
P0A8F8	UvrABC system protein B	)	BACCA		658	UniRef50_P56981
UvrABC system protein B		GEOKA		658
UvrABC system protein B		PROMH		669
UvrABC system protein B		METST		649
UvrABC system protein B		STRTD		668
+19882

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X03678 Genomic DNA Translation: CAA27314.1 X03722 Genomic DNA Translation: CAA27357.1 U00096 Genomic DNA Translation: AAC73866.1 AP009048 Genomic DNA Translation: BAA35437.2
PIRⁱ	A93613 BVECUB
RefSeqⁱ	NP_415300.1, NC_000913.3 WP_000042533.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1E52	NMR	-	A/B	619-673	[»]
	1QOJ	X-ray	3.00	A/B	619-673	[»]
ProteinModelPortalⁱ	P0A8F8
SMRⁱ	P0A8F8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259957, 140 interactors
ComplexPortalⁱ	CPX-2151 UvrAB complex CPX-2152 UvrB complex CPX-2153 UvrBC complex
DIPⁱ	DIP-48012N
IntActⁱ	P0A8F8, 32 interactors
STRINGⁱ	316385.ECDH10B_0847

Proteomic databases

PaxDbⁱ	P0A8F8
PRIDEⁱ	P0A8F8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73866; AAC73866; b0779 BAA35437; BAA35437; BAA35437
GeneIDⁱ	945385
KEGGⁱ	ecj:JW0762 eco:b0779
PATRICⁱ	fig\|1411691.4.peg.1499

Organism-specific databases

EchoBASEⁱ	EB1055
EcoGeneⁱ	EG11062 uvrB

Phylogenomic databases

eggNOGⁱ	ENOG4105CCW Bacteria COG0556 LUCA
HOGENOMⁱ	HOG000073580
InParanoidⁱ	P0A8F8
KOⁱ	K03702
OMAⁱ	RYMHSEI
PhylomeDBⁱ	P0A8F8

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11062-MONOMER MetaCyc:EG11062-MONOMER

BioCycⁱ

EcoCyc:EG11062-MONOMER
MetaCyc:EG11062-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0A8F8
Protein Ontology More...PROⁱ	PR:P0A8F8

Family and domain databases

CDDⁱ	cd00079 HELICc, 1 hit
HAMAPⁱ	MF_00204 UvrB, 1 hit
InterProⁱ	View protein in InterPro IPR006935 Helicase/UvrB_N IPR014001 Helicase_ATP-bd IPR001650 Helicase_C IPR027417 P-loop_NTPase IPR001943 UVR_dom IPR036876 UVR_dom_sf IPR004807 UvrB IPR024759 UvrB_YAD/RRR_dom
PANTHERⁱ	PTHR24029 PTHR24029, 1 hit
Pfamⁱ	View protein in Pfam PF00271 Helicase_C, 1 hit PF04851 ResIII, 1 hit PF02151 UVR, 1 hit PF12344 UvrB, 1 hit
SMARTⁱ	View protein in SMART SM00487 DEXDc, 1 hit SM00490 HELICc, 1 hit
SUPFAMⁱ	SSF46600 SSF46600, 1 hit SSF52540 SSF52540, 3 hits
TIGRFAMsⁱ	TIGR00631 uvrb, 1 hit
PROSITEⁱ	View protein in PROSITE PS51192 HELICASE_ATP_BIND_1, 1 hit PS51194 HELICASE_CTER, 1 hit PS50151 UVR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UVRB_ECOLI
Accessionⁱ	P0A8F8Primary (citable) accession number: P0A8F8 Secondary accession number(s): P07025
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	January 23, 2007
	Last modified:	October 10, 2018
	This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A8F8 (UVRB_ECOLI)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

UvrABC system protein B

uvrB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Sites

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ