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UniProtKB - P0A8F0 (UPP_ECOLI)
Protein
Uracil phosphoribosyltransferase
Gene
upp
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.
Catalytic activityi
- EC:2.4.2.91 Publication
Cofactori
Mg2+Note: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.
Activity regulationi
Allosterically activated by GTP.1 Publication
Kineticsi
- KM=0.53 µM for uracil1 Publication
- KM=58 µM for 5-phospho-alpha-D-ribose 1-diphosphate1 Publication
: UMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil. This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | 5-phospho-alpha-D-ribose 1-diphosphateBy similarity | 1 | |
Binding sitei | 103 | 5-phospho-alpha-D-ribose 1-diphosphateBy similarity | 1 | |
Binding sitei | 193 | Uracil; via amide nitrogenBy similarity | 1 | |
Binding sitei | 199 | 5-phospho-alpha-D-ribose 1-diphosphateBy similarity | 1 |
GO - Molecular functioni
- GTP binding Source: UniProtKB-KW
- guanosine tetraphosphate binding Source: EcoCyc
- identical protein binding Source: IntAct
- magnesium ion binding Source: UniProtKB-UniRule
- uracil phosphoribosyltransferase activity Source: EcoCyc
GO - Biological processi
- UMP salvage Source: UniProtKB-UniPathway
- uracil salvage Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Glycosyltransferase, Transferase |
Ligand | GTP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:URACIL-PRIBOSYLTRANS-MONOMER |
SABIO-RKi | P0A8F0 |
UniPathwayi | UPA00574;UER00636 |
Names & Taxonomyi
Protein namesi | Recommended name: Uracil phosphoribosyltransferase (EC:2.4.2.9)Alternative name(s): UMP pyrophosphorylase UPRTase |
Gene namesi | Name:upp Synonyms:uraP Ordered Locus Names:b2498, JW2483 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 131 | P → D: 60-fold reduction of the catalytic rate, and large decrease in uracil-binding affinity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2233621 |
DrugCentrali | P0A8F0 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120824 | 1 – 208 | Uracil phosphoribosyltransferaseAdd BLAST | 208 |
Proteomic databases
jPOSTi | P0A8F0 |
PaxDbi | P0A8F0 |
PRIDEi | P0A8F0 |
2D gel databases
SWISS-2DPAGEi | P0A8F0 |
Expressioni
Inductioni
By pyrimidine starvation.
Interactioni
Subunit structurei
Homodimer or homotrimer in the absence of substrates, and homopentamer or homohexamer in the presence of substrates.
1 PublicationBinary interactionsi
P0A8F0
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-909572,EBI-909572 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261438, 30 interactors 851318, 1 interactor |
DIPi | DIP-36228N |
IntActi | P0A8F0, 6 interactors |
STRINGi | 511145.b2498 |
Chemistry databases
BindingDBi | P0A8F0 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A8F0 |
SMRi | P0A8F0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A8F0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 138 | 5-phospho-alpha-D-ribose 1-diphosphate bindingBy similarity | 9 | |
Regioni | 198 – 200 | Uracil bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the UPRTase family.Curated
Phylogenomic databases
eggNOGi | COG0035, Bacteria |
HOGENOMi | CLU_067096_2_2_6 |
InParanoidi | P0A8F0 |
OMAi | TYATRMP |
PhylomeDBi | P0A8F0 |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
HAMAPi | MF_01218_B, Upp_B, 1 hit |
InterProi | View protein in InterPro IPR000836, PRibTrfase_dom IPR029057, PRTase-like IPR034332, Upp_B IPR005765, Ura_phspho_trans |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR01091, upp, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8F0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIVEVKHPL VKHKLGLMRE QDISTKRFRE LASEVGSLLT YEATADLETE
60 70 80 90 100
KVTIEGWNGP VEIDQIKGKK ITVVPILRAG LGMMDGVLEN VPSARISVVG
110 120 130 140 150
MYRNEETLEP VPYFQKLVSN IDERMALIVD PMLATGGSVI ATIDLLKKAG
160 170 180 190 200
CSSIKVLVLV AAPEGIAALE KAHPDVELYT ASIDQGLNEH GYIIPGLGDA
GDKIFGTK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57104 Genomic DNA Translation: CAA40388.1 U00096 Genomic DNA Translation: AAC75551.2 AP009048 Genomic DNA Translation: BAA16386.1 |
PIRi | A65026 |
RefSeqi | NP_416993.2, NC_000913.3 WP_001295473.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75551; AAC75551; b2498 BAA16386; BAA16386; BAA16386 |
GeneIDi | 67416878 946979 |
KEGGi | ecj:JW2483 eco:b2498 |
PATRICi | fig|1411691.4.peg.4241 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57104 Genomic DNA Translation: CAA40388.1 U00096 Genomic DNA Translation: AAC75551.2 AP009048 Genomic DNA Translation: BAA16386.1 |
PIRi | A65026 |
RefSeqi | NP_416993.2, NC_000913.3 WP_001295473.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EHJ | X-ray | 2.80 | A/B/C/D | 2-208 | [»] | |
AlphaFoldDBi | P0A8F0 | |||||
SMRi | P0A8F0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261438, 30 interactors 851318, 1 interactor |
DIPi | DIP-36228N |
IntActi | P0A8F0, 6 interactors |
STRINGi | 511145.b2498 |
Chemistry databases
BindingDBi | P0A8F0 |
ChEMBLi | CHEMBL2233621 |
DrugCentrali | P0A8F0 |
2D gel databases
SWISS-2DPAGEi | P0A8F0 |
Proteomic databases
jPOSTi | P0A8F0 |
PaxDbi | P0A8F0 |
PRIDEi | P0A8F0 |
Genome annotation databases
EnsemblBacteriai | AAC75551; AAC75551; b2498 BAA16386; BAA16386; BAA16386 |
GeneIDi | 67416878 946979 |
KEGGi | ecj:JW2483 eco:b2498 |
PATRICi | fig|1411691.4.peg.4241 |
Organism-specific databases
EchoBASEi | EB1308 |
Phylogenomic databases
eggNOGi | COG0035, Bacteria |
HOGENOMi | CLU_067096_2_2_6 |
InParanoidi | P0A8F0 |
OMAi | TYATRMP |
PhylomeDBi | P0A8F0 |
Enzyme and pathway databases
UniPathwayi | UPA00574;UER00636 |
BioCyci | EcoCyc:URACIL-PRIBOSYLTRANS-MONOMER |
SABIO-RKi | P0A8F0 |
Miscellaneous databases
EvolutionaryTracei | P0A8F0 |
PROi | PR:P0A8F0 |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
HAMAPi | MF_01218_B, Upp_B, 1 hit |
InterProi | View protein in InterPro IPR000836, PRibTrfase_dom IPR029057, PRTase-like IPR034332, Upp_B IPR005765, Ura_phspho_trans |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR01091, upp, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UPP_ECOLI | |
Accessioni | P0A8F0Primary (citable) accession number: P0A8F0 Secondary accession number(s): P25532, P78095, Q8XAC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families