UniProtKB - P0A8A4 (PSRP_ECOLI)
Protein
Phosphoenolpyruvate synthase regulatory protein
Gene
ppsR
Organism
Escherichia coli (strain K12)
Status
Functioni
Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the phosphoenolpyruvate synthase (PEPS) by catalyzing its phosphorylation/dephosphorylation.UniRule annotation1 Publication
Catalytic activityi
- [pyruvate, water dikinase] + ADP = [pyruvate, water dikinase]-phosphate + AMP + H+UniRule annotation1 PublicationEC:2.7.11.33UniRule annotation1 Publication
- [pyruvate, water dikinase]-phosphate + H+ + phosphate = [pyruvate, water dikinase] + diphosphateUniRule annotation1 PublicationEC:2.7.4.28UniRule annotation1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 157 – 164 | ADPUniRule annotation | 8 |
GO - Molecular functioni
- ADP binding Source: UniProtKB-UniRule
- ATP binding Source: InterPro
- enzyme regulator activity Source: EcoCyc
- phosphotransferase activity, phosphate group as acceptor Source: UniProtKB-UniRule
- protein serine/threonine kinase activity Source: UniProtKB-UniRule
GO - Biological processi
- protein dephosphorylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11132-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoenolpyruvate synthase regulatory proteinUniRule annotation (EC:2.7.11.33UniRule annotation, EC:2.7.4.28UniRule annotation)Short name: PEP synthase regulatory proteinUniRule annotation Short name: PSRPUniRule annotation Alternative name(s): Pyruvate, water dikinase regulatory proteinUniRule annotation |
Gene namesi | Name:ppsRUniRule annotation Synonyms:ydiA Ordered Locus Names:b1703, JW1693 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000196659 | 1 – 277 | Phosphoenolpyruvate synthase regulatory proteinAdd BLAST | 277 |
Proteomic databases
jPOSTi | P0A8A4 |
PaxDbi | P0A8A4 |
PRIDEi | P0A8A4 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationBinary interactionsi
P0A8A4
With | #Exp. | IntAct |
---|---|---|
rluD [P33643] | 3 | EBI-548302,EBI-558026 |
Protein-protein interaction databases
BioGRIDi | 4260288, 14 interactors 850567, 4 interactors |
DIPi | DIP-47885N |
IntActi | P0A8A4, 14 interactors |
STRINGi | 511145.b1703 |
Family & Domainsi
Sequence similaritiesi
Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PSRP subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG1806, Bacteria |
HOGENOMi | CLU_046206_1_0_6 |
InParanoidi | P0A8A4 |
PhylomeDBi | P0A8A4 |
Family and domain databases
HAMAPi | MF_01062, PSRP, 1 hit |
InterProi | View protein in InterPro IPR005177, Kinase-pyrophosphorylase IPR026530, PSRP |
PANTHERi | PTHR31756, PTHR31756, 1 hit |
Pfami | View protein in Pfam PF03618, Kinase-PPPase, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A8A4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNAVDRHVF YISDGTAITA EVLGHAVMSQ FPVTISSITL PFVENESRAR
60 70 80 90 100
AVKDQIDAIY HQTGVRPLVF YSIVLPEIRA IILQSEGFCQ DIVQALVAPL
110 120 130 140 150
QQEMKLDPTP IAHRTHGLNP NNLNKYDARI AAIDYTLAHD DGISLRNLDQ
160 170 180 190 200
AQVILLGVSR CGKTPTSLYL AMQFGIRAAN YPFIADDMDN LVLPASLKPL
210 220 230 240 250
QHKLFGLTID PERLAAIREE RRENSRYASL RQCRMEVAEV EALYRKNQIP
260 270
WINSTNYSVE EIATKILDIM GLSRRMY
Sequence cautioni
The sequence M69116 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74773.1 AP009048 Genomic DNA Translation: BAA15472.2 M69116 Genomic DNA No translation available. M38266 Genomic DNA Translation: AAA23496.1 V00261 Genomic DNA No translation available. |
PIRi | G64928, QQECAD |
RefSeqi | NP_416218.1, NC_000913.3 WP_000368046.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74773; AAC74773; b1703 BAA15472; BAA15472; BAA15472 |
GeneIDi | 49584541 946207 |
KEGGi | ecj:JW1693 eco:b1703 |
PATRICi | fig|1411691.4.peg.554 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74773.1 AP009048 Genomic DNA Translation: BAA15472.2 M69116 Genomic DNA No translation available. M38266 Genomic DNA Translation: AAA23496.1 V00261 Genomic DNA No translation available. |
PIRi | G64928, QQECAD |
RefSeqi | NP_416218.1, NC_000913.3 WP_000368046.1, NZ_SSZK01000001.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260288, 14 interactors 850567, 4 interactors |
DIPi | DIP-47885N |
IntActi | P0A8A4, 14 interactors |
STRINGi | 511145.b1703 |
Proteomic databases
jPOSTi | P0A8A4 |
PaxDbi | P0A8A4 |
PRIDEi | P0A8A4 |
Genome annotation databases
EnsemblBacteriai | AAC74773; AAC74773; b1703 BAA15472; BAA15472; BAA15472 |
GeneIDi | 49584541 946207 |
KEGGi | ecj:JW1693 eco:b1703 |
PATRICi | fig|1411691.4.peg.554 |
Organism-specific databases
EchoBASEi | EB1122 |
Phylogenomic databases
eggNOGi | COG1806, Bacteria |
HOGENOMi | CLU_046206_1_0_6 |
InParanoidi | P0A8A4 |
PhylomeDBi | P0A8A4 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11132-MONOMER |
Miscellaneous databases
PROi | PR:P0A8A4 |
Family and domain databases
HAMAPi | MF_01062, PSRP, 1 hit |
InterProi | View protein in InterPro IPR005177, Kinase-pyrophosphorylase IPR026530, PSRP |
PANTHERi | PTHR31756, PTHR31756, 1 hit |
Pfami | View protein in Pfam PF03618, Kinase-PPPase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSRP_ECOLI | |
Accessioni | P0A8A4Primary (citable) accession number: P0A8A4 Secondary accession number(s): P03822, P46137, P76203 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 7, 2005 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families