UniProtKB - P0A898 (YBEY_ECOLI)
Endoribonuclease YbeY
ybeY
Functioni
Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'- and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.
UniRule annotation4 PublicationsCofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 114 | Zinc; catalyticCurated | 1 | |
Metal bindingi | 118 | Zinc; catalyticCurated | 1 | |
Metal bindingi | 124 | Zinc; catalyticCurated | 1 |
GO - Molecular functioni
- endoribonuclease activity, producing 3'-phosphomonoesters Source: EcoCyc
- metalloendopeptidase activity Source: InterPro
- nickel cation binding Source: EcoCyc
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- endonucleolytic cleavage involved in rRNA processing Source: EcoCyc
- maturation of SSU-rRNA Source: EcoCyc
- response to heat Source: EcoCyc
- ribosomal small subunit biogenesis Source: EcoCyc
- ribosome biogenesis Source: UniProtKB
- rRNA processing Source: UniProtKB
- transcription antitermination Source: EcoCyc
- translation Source: EcoCyc
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G6362-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ybeYUniRule annotation Ordered Locus Names:b0659, JW0656 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 59 | R → A or E: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 114 | H → A: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 118 | H → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 124 | H → A: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102449 | 1 – 155 | Endoribonuclease YbeYAdd BLAST | 155 |
Proteomic databases
jPOSTi | P0A898 |
PaxDbi | P0A898 |
PRIDEi | P0A898 |
Expressioni
Inductioni
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261388, 68 interactors |
DIPi | DIP-48144N |
IntActi | P0A898, 15 interactors |
STRINGi | 511145.b0659 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0A898 |
SMRi | P0A898 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A898 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0319, Bacteria |
HOGENOMi | CLU_106710_0_1_6 |
InParanoidi | P0A898 |
OMAi | RMRIHPL |
PhylomeDBi | P0A898 |
Family and domain databases
Gene3Di | 3.40.390.30, 1 hit |
HAMAPi | MF_00009, Endoribonucl_YbeY, 1 hit |
InterProi | View protein in InterPro IPR023091, MetalPrtase_cat_dom_sf_prd IPR002036, YbeY IPR020549, YbeY_CS |
PANTHERi | PTHR46986, PTHR46986, 1 hit |
Pfami | View protein in Pfam PF02130, YbeY, 1 hit |
TIGRFAMsi | TIGR00043, TIGR00043, 1 hit |
PROSITEi | View protein in PROSITE PS01306, UPF0054, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSQVILDLQL ACEDNSGLPE ESQFQTWLNA VIPQFQEESE VTIRVVDTAE
60 70 80 90 100
SHSLNLTYRG KDKPTNVLSF PFEVPPGMEM SLLGDLVICR QVVEKEAQEQ
110 120 130 140 150
GKPLEAHWAH MVVHGSLHLL GYDHIEDDEA EEMEALETEI MLALGYEDPY
IAEKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40861.1 U00096 Genomic DNA Translation: AAC73760.1 AP009048 Genomic DNA Translation: BAA35314.1 |
PIRi | A64801 |
RefSeqi | NP_415192.1, NC_000913.3 WP_000084469.1, NZ_SSZK01000037.1 |
Genome annotation databases
EnsemblBacteriai | AAC73760; AAC73760; b0659 BAA35314; BAA35314; BAA35314 |
GeneIDi | 67416301 946430 |
KEGGi | ecj:JW0656 eco:b0659 |
PATRICi | fig|1411691.4.peg.1609 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40861.1 U00096 Genomic DNA Translation: AAC73760.1 AP009048 Genomic DNA Translation: BAA35314.1 |
PIRi | A64801 |
RefSeqi | NP_415192.1, NC_000913.3 WP_000084469.1, NZ_SSZK01000037.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XM5 | X-ray | 2.70 | A/B/C/D | 1-155 | [»] | |
AlphaFoldDBi | P0A898 | |||||
SMRi | P0A898 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261388, 68 interactors |
DIPi | DIP-48144N |
IntActi | P0A898, 15 interactors |
STRINGi | 511145.b0659 |
Proteomic databases
jPOSTi | P0A898 |
PaxDbi | P0A898 |
PRIDEi | P0A898 |
Protocols and materials databases
DNASUi | 946430 |
Genome annotation databases
EnsemblBacteriai | AAC73760; AAC73760; b0659 BAA35314; BAA35314; BAA35314 |
GeneIDi | 67416301 946430 |
KEGGi | ecj:JW0656 eco:b0659 |
PATRICi | fig|1411691.4.peg.1609 |
Organism-specific databases
EchoBASEi | EB3419 |
Phylogenomic databases
eggNOGi | COG0319, Bacteria |
HOGENOMi | CLU_106710_0_1_6 |
InParanoidi | P0A898 |
OMAi | RMRIHPL |
PhylomeDBi | P0A898 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6362-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A898 |
PROi | PR:P0A898 |
Family and domain databases
Gene3Di | 3.40.390.30, 1 hit |
HAMAPi | MF_00009, Endoribonucl_YbeY, 1 hit |
InterProi | View protein in InterPro IPR023091, MetalPrtase_cat_dom_sf_prd IPR002036, YbeY IPR020549, YbeY_CS |
PANTHERi | PTHR46986, PTHR46986, 1 hit |
Pfami | View protein in Pfam PF02130, YbeY, 1 hit |
TIGRFAMsi | TIGR00043, TIGR00043, 1 hit |
PROSITEi | View protein in PROSITE PS01306, UPF0054, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | YBEY_ECOLI | |
Accessioni | P0A898Primary (citable) accession number: P0A898 Secondary accession number(s): P77385 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | April 26, 2005 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families