UniProtKB - P0A890 (TUSA_ECOLI)
Protein
Sulfur carrier protein TusA
Gene
tusA
Organism
Escherichia coli (strain K12)
Status
Functioni
Sulfur carrier protein involved in sulfur trafficking in the cell. Part of a sulfur-relay system required for 2-thiolation during synthesis of 2-thiouridine of the modified wobble base 5-methylaminomethyl-2-thiouridine (mnm5s2U) in tRNA (PubMed:16387657). Interacts with IscS and stimulates its cysteine desulfurase activity (PubMed:16387657, PubMed:23281480). Accepts an activated sulfur from IscS, which is then transferred to TusD, and thus determines the direction of sulfur flow from IscS to 2-thiouridine formation (PubMed:16387657). Also appears to be involved in sulfur transfer for the biosynthesis of molybdopterin (PubMed:23281480). Seems to affect the stability of sigma-S, particularly during the logarithmic growth phase (PubMed:9555915).3 Publications
: tRNA modification Pathwayi
This protein is involved in tRNA modification.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 19 | Cysteine persulfide intermediateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- sulfur carrier activity Source: EcoCyc
GO - Biological processi
- Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
- tRNA wobble position uridine thiolation Source: EcoCyc
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12216-MONOMER MetaCyc:EG12216-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfur carrier protein TusA1 PublicationUniRule annotationAlternative name(s): Sulfur mediator TusA1 PublicationUniRule annotation Sulfur transfer protein TusA1 PublicationUniRule annotation tRNA 2-thiouridine synthesizing protein A1 PublicationUniRule annotation |
Gene namesi | Name:tusA1 PublicationUniRule annotation Synonyms:sirA, yhhP Ordered Locus Names:b3470, JW3435 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene are not capable of growing in standard laboratory rich medium (i.e., Luria broth), and show filamentous shape (PubMed:9555915). FtsZ-ring formation appears to be severely impaired in tusA-deficient cells, resulting in the formation of a non-divided filamentous cell (PubMed:10830496). A tusA deletion mutant lacks the 2-thio modification of mnm5s2U in tRNA and has a severe growth defect (PubMed:16387657). Deletion of tusA has a pleiotropic effect on transcription, including increased expression of molybdenum cofactor biosynthesis genes (moaABCDE operon), but leads to reduced activity of molybdoenzymes, an overall low molybdopterin content and an accumulation of the molybdopterin precursor cyclic pyranopterin monophopshate (cPMP) (PubMed:23281480).4 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | C → S: Unable to restore mnm5s2U formation in a tusA-deficient mutant. Unable to accept sulfur from IscS. 1 Publication | 1 | |
Mutagenesisi | 21 | E → K in sirA1; affects the stability of sigma-S during the logarithmic growth phase. 1 Publication | 1 | |
Mutagenesisi | 56 | C → S: Able to restore mnm5s2U formation in a tusA-deficient mutant. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159033 | 1 – 81 | Sulfur carrier protein TusAAdd BLAST | 81 |
Proteomic databases
jPOSTi | P0A890 |
PaxDbi | P0A890 |
PRIDEi | P0A890 |
Interactioni
Subunit structurei
Interacts with IscS.
UniRule annotation2 PublicationsBinary interactionsi
P0A890
With | #Exp. | IntAct |
---|---|---|
iscS [P0A6B7] | 7 | EBI-561780,EBI-550055 |
Protein-protein interaction databases
BioGRIDi | 852283, 1 interactor |
ComplexPortali | CPX-2139, IscS-TusA complex |
DIPi | DIP-48202N |
IntActi | P0A890, 16 interactors |
STRINGi | 511145.b3470 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A890 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A890 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sulfur carrier protein TusA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0425, Bacteria |
HOGENOMi | CLU_165255_5_0_6 |
InParanoidi | P0A890 |
PhylomeDBi | P0A890 |
Family and domain databases
CDDi | cd03423, SirA, 1 hit |
Gene3Di | 3.30.110.40, 1 hit |
HAMAPi | MF_00413, Thiourid_synth_A, 1 hit |
InterProi | View protein in InterPro IPR022931, Sulphur_carrier_TusA IPR001455, TusA-like IPR036868, TusA-like_sf |
Pfami | View protein in Pfam PF01206, TusA, 1 hit |
SUPFAMi | SSF64307, SSF64307, 1 hit |
PROSITEi | View protein in PROSITE PS01148, UPF0033, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A890-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQPGETLL IIADDPATTR
60 70 80
DIPGFCTFME HELVAKETDG LPYRYLIRKG G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18445.1 U00096 Genomic DNA Translation: AAC76495.1 AP009048 Genomic DNA Translation: BAE77823.1 |
PIRi | S47689 |
RefSeqi | NP_417927.1, NC_000913.3 WP_000130621.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76495; AAC76495; b3470 BAE77823; BAE77823; BAE77823 |
GeneIDi | 57729267 947974 |
KEGGi | ecj:JW3435 eco:b3470 |
PATRICi | fig|1411691.4.peg.3255 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18445.1 U00096 Genomic DNA Translation: AAC76495.1 AP009048 Genomic DNA Translation: BAE77823.1 |
PIRi | S47689 |
RefSeqi | NP_417927.1, NC_000913.3 WP_000130621.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DCJ | NMR | - | A | 1-81 | [»] | |
SMRi | P0A890 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 852283, 1 interactor |
ComplexPortali | CPX-2139, IscS-TusA complex |
DIPi | DIP-48202N |
IntActi | P0A890, 16 interactors |
STRINGi | 511145.b3470 |
Proteomic databases
jPOSTi | P0A890 |
PaxDbi | P0A890 |
PRIDEi | P0A890 |
Genome annotation databases
EnsemblBacteriai | AAC76495; AAC76495; b3470 BAE77823; BAE77823; BAE77823 |
GeneIDi | 57729267 947974 |
KEGGi | ecj:JW3435 eco:b3470 |
PATRICi | fig|1411691.4.peg.3255 |
Organism-specific databases
EchoBASEi | EB2130 |
Phylogenomic databases
eggNOGi | COG0425, Bacteria |
HOGENOMi | CLU_165255_5_0_6 |
InParanoidi | P0A890 |
PhylomeDBi | P0A890 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12216-MONOMER MetaCyc:EG12216-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A890 |
PROi | PR:P0A890 |
Family and domain databases
CDDi | cd03423, SirA, 1 hit |
Gene3Di | 3.30.110.40, 1 hit |
HAMAPi | MF_00413, Thiourid_synth_A, 1 hit |
InterProi | View protein in InterPro IPR022931, Sulphur_carrier_TusA IPR001455, TusA-like IPR036868, TusA-like_sf |
Pfami | View protein in Pfam PF01206, TusA, 1 hit |
SUPFAMi | SSF64307, SSF64307, 1 hit |
PROSITEi | View protein in PROSITE PS01148, UPF0033, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TUSA_ECOLI | |
Accessioni | P0A890Primary (citable) accession number: P0A890 Secondary accession number(s): P37618, Q2M7D3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families