ubiE

Functionⁱ

Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).UniRule annotation1 Publication

Catalytic activityⁱ

A demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine.UniRule annotation1 Publication

S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol.UniRule annotation1 Publication

Pathwayⁱ: menaquinone biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)

This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Pathwayⁱ: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	74	S-adenosyl-L-methionineUniRule annotation	1
Binding siteⁱ	95	S-adenosyl-L-methionineUniRule annotation	1
Binding siteⁱ	140	S-adenosyl-L-methionineUniRule annotation	1

GO - Molecular functionⁱ

2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 1444716
S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity Source: UniProtKB-EC
S-adenosylmethionine-dependent methyltransferase activity
Source: EcoCyc
Inferred from sequence or structural similarityⁱ
- 9045837

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aerobic respiration Source: UniProtKB-UniRule
menaquinone biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 1444716
ubiquinone biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 4323297

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Menaquinone biosynthesis, Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	EcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER
BRENDAⁱ	2.1.1.163 2026
UniPathwayⁱ	UPA00079;UER00169 UPA00232

Chemistry databases

SwissLipidsⁱ	SLP:000001505

SwissLipidsⁱ

SLP:000001505

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEUniRule annotation (EC:2.1.1.163UniRule annotation1 Publication, EC:2.1.1.201UniRule annotation1 Publication) Alternative name(s): 2-methoxy-6-polyprenyl-1,4-benzoquinol methylaseUniRule annotation Demethylmenaquinone methyltransferaseUniRule annotation
Gene namesⁱ	Name:ubiE1 PublicationUniRule annotation Synonyms:yigO Ordered Locus Names:b3833, JW5581
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11473 ubiE

EcoGeneⁱ

EG11473 ubiE

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	142	G → D in ubiE401; defective. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000193274	1 – 251	Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEAdd BLAST		251

Proteomic databases

EPDⁱ	P0A887
PaxDbⁱ	P0A887
PRIDEⁱ	P0A887

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4261082, 536 interactors
Database of interacting proteins More...DIPⁱ	DIP-31831N
IntActⁱ	P0A887, 6 interactors
STRINGⁱ	316385.ECDH10B_4024

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0A887
SMRⁱ	P0A887
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	123 – 124	S-adenosyl-L-methionine bindingUniRule annotation		2

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105DDZ Bacteria COG2226 LUCA
HOGENOMⁱ	HOG000249463
InParanoidⁱ	P0A887
KEGG Orthology (KO) More...KOⁱ	K03183
PhylomeDBⁱ	P0A887

Family and domain databases

HAMAPⁱ	MF_01813 MenG_UbiE_methyltr, 1 hit
InterProⁱ	View protein in InterPro IPR029063 SAM-dependent_MTases IPR004033 UbiE/COQ5_MeTrFase IPR023576 UbiE/COQ5_MeTrFase_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01209 Ubie_methyltran, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
TIGRFAMsⁱ	TIGR01934 MenG_MenH_UbiE, 1 hit
PROSITEⁱ	View protein in PROSITE PS51608 SAM_MT_UBIE, 1 hit PS01183 UBIE_1, 1 hit PS01184 UBIE_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0A887-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL 
        60         70         80         90        100
WKRFTIDCSG VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM 
       110        120        130        140        150
LKMGREKLRN IGVIGNVEYV QANAEALPFP DNTFDCITIS FGLRNVTDKD 
       160        170        180        190        200
KALRSMYRVL KPGGRLLVLE FSKPIIEPLS KAYDAYSFHV LPRIGSLVAN 
       210        220        230        240        250
DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA GVVALHRGYK 

F

Length:251

Mass (Da):28,073

Last modified:June 7, 2005 - v1

Checksum:ⁱB1EEE209BDC68F5E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M87049 Genomic DNA Translation: AAA67628.1 U00096 Genomic DNA Translation: AAT48227.1 AP009048 Genomic DNA Translation: BAE77468.1
PIRⁱ	B65188
NCBI Reference Sequences More...RefSeqⁱ	WP_000227958.1, NZ_LN832404.1 YP_026269.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaⁱ	AAT48227; AAT48227; b3833 BAE77468; BAE77468; BAE77468
GeneIDⁱ	948926
KEGGⁱ	ecj:JW5581 eco:b3833
PATRICⁱ	fig\|1411691.4.peg.2875

Protein	Similar proteins		Species	Score	Length	Source
P0A887	Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO24		251	UniRef100_A7ZU40
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO27		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO55		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO57		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO5E		251
+174

Protein	Similar proteins		Species	Score	Length	Source
P0A887	Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO24		251	UniRef90_A7ZU40
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO27		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO55		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO57		251
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		ECO5E		251
+178

Protein	Similar proteins		Species	Score	Length	Source
P0A887	Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		XANCP		253	UniRef50_Q8P558
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		STRM5		253
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		STRMK		253
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		XANAC		253
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE		XANOM		253
+1594

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M87049 Genomic DNA Translation: AAA67628.1 U00096 Genomic DNA Translation: AAT48227.1 AP009048 Genomic DNA Translation: BAE77468.1
PIRⁱ	B65188
RefSeqⁱ	WP_000227958.1, NZ_LN832404.1 YP_026269.1, NC_000913.3

3D structure databases

ProteinModelPortalⁱ	P0A887
SMRⁱ	P0A887
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261082, 536 interactors
DIPⁱ	DIP-31831N
IntActⁱ	P0A887, 6 interactors
STRINGⁱ	316385.ECDH10B_4024

Chemistry databases

SwissLipidsⁱ	SLP:000001505

SwissLipidsⁱ

SLP:000001505

Proteomic databases

EPDⁱ	P0A887
PaxDbⁱ	P0A887
PRIDEⁱ	P0A887

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAT48227; AAT48227; b3833 BAE77468; BAE77468; BAE77468
GeneIDⁱ	948926
KEGGⁱ	ecj:JW5581 eco:b3833
PATRICⁱ	fig\|1411691.4.peg.2875

Organism-specific databases

EchoBASEⁱ	EB1441
EcoGeneⁱ	EG11473 ubiE

Phylogenomic databases

eggNOGⁱ	ENOG4105DDZ Bacteria COG2226 LUCA
HOGENOMⁱ	HOG000249463
InParanoidⁱ	P0A887
KOⁱ	K03183
PhylomeDBⁱ	P0A887

Enzyme and pathway databases

UniPathwayⁱ	UPA00079;UER00169 UPA00232
BioCycⁱ	EcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER
BRENDAⁱ	2.1.1.163 2026

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0A887

PROⁱ

PR:P0A887

Family and domain databases

HAMAPⁱ	MF_01813 MenG_UbiE_methyltr, 1 hit
InterProⁱ	View protein in InterPro IPR029063 SAM-dependent_MTases IPR004033 UbiE/COQ5_MeTrFase IPR023576 UbiE/COQ5_MeTrFase_CS
Pfamⁱ	View protein in Pfam PF01209 Ubie_methyltran, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
TIGRFAMsⁱ	TIGR01934 MenG_MenH_UbiE, 1 hit
PROSITEⁱ	View protein in PROSITE PS51608 SAM_MT_UBIE, 1 hit PS01183 UBIE_1, 1 hit PS01184 UBIE_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBIE_ECOLI
Accessionⁱ	P0A887Primary (citable) accession number: P0A887 Secondary accession number(s): P27851, Q2M8D8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
	Last sequence update:	June 7, 2005
	Last modified:	November 7, 2018
	This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A887 (UBIE_ECOLI)

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Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: menaquinone biosynthesis

Pathwayi: ubiquinone biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: menaquinone biosynthesis

Pathwayⁱ: ubiquinone biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ