UniProtKB - P0A887 (UBIE_ECOLI)
Protein
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
Gene
ubiE
Organism
Escherichia coli (strain K12)
Status
Functioni
Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) (PubMed:9045837). In vitro, can use demethylphylloquinol, an intermediate in the biosynthesis of phylloquinone (vitamin K1) in plants and cyanobacteria (PubMed:26023160).2 Publications
Catalytic activityi
- a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H+ + S-adenosyl-L-homocysteineUniRule annotation1 PublicationEC:2.1.1.163UniRule annotation1 Publication
- a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + H+ + S-adenosyl-L-homocysteineUniRule annotation1 PublicationEC:2.1.1.201UniRule annotation1 Publication
: menaquinone biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA), 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
- Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE), Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.
Pathwayi: ubiquinone biosynthesis
This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 95 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 140 | S-adenosyl-L-methionineUniRule annotation | 1 |
GO - Molecular functioni
- 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity Source: EcoCyc
- demethylmenaquinone methyltransferase activity Source: UniProtKB-EC
- methyltransferase activity Source: GO_Central
- quinone cofactor methyltransferase activity Source: EcoCyc
- S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity Source: UniProtKB-EC
- S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity Source: UniProtKB-EC
- S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- aerobic respiration Source: UniProtKB-UniRule
- menaquinone biosynthetic process Source: EcoCyc
- methylation Source: UniProtKB-KW
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Menaquinone biosynthesis, Ubiquinone biosynthesis |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER |
BRENDAi | 2.1.1.163, 2026 |
UniPathwayi | UPA00079;UER00169 UPA00232 |
Chemistry databases
SwissLipidsi | SLP:000001505 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEUniRule annotation (EC:2.1.1.163UniRule annotation1 Publication, EC:2.1.1.201UniRule annotation1 Publication)Alternative name(s): 2-methoxy-6-polyprenyl-1,4-benzoquinol methylaseUniRule annotation Demethylmenaquinone methyltransferaseUniRule annotation |
Gene namesi | Name:ubiE1 PublicationUniRule annotation Synonyms:yigO Ordered Locus Names:b3833, JW5581 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
- ubiquinone biosynthesis complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 142 | G → D in ubiE401; defective. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193274 | 1 – 251 | Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEAdd BLAST | 251 |
Proteomic databases
jPOSTi | P0A887 |
PaxDbi | P0A887 |
PRIDEi | P0A887 |
Interactioni
Subunit structurei
Component of the Ubi complex metabolon, which regroups five ubiquinone biosynthesis proteins (UbiE, UbiF, UbiG, UbiH and UbiI) and two accessory factors (UbiK and the lipid-binding protein UbiJ).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261082, 536 interactors 853202, 2 interactors |
DIPi | DIP-31831N |
IntActi | P0A887, 6 interactors |
STRINGi | 511145.b3833 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 123 – 124 | S-adenosyl-L-methionine bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2226, Bacteria |
HOGENOMi | CLU_037990_0_0_6 |
InParanoidi | P0A887 |
PhylomeDBi | P0A887 |
Family and domain databases
HAMAPi | MF_01813, MenG_UbiE_methyltr, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR004033, UbiE/COQ5_MeTrFase IPR023576, UbiE/COQ5_MeTrFase_CS |
Pfami | View protein in Pfam PF01209, Ubie_methyltran, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR01934, MenG_MenH_UbiE, 1 hit |
PROSITEi | View protein in PROSITE PS51608, SAM_MT_UBIE, 1 hit PS01183, UBIE_1, 1 hit PS01184, UBIE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A887-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL
60 70 80 90 100
WKRFTIDCSG VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM
110 120 130 140 150
LKMGREKLRN IGVIGNVEYV QANAEALPFP DNTFDCITIS FGLRNVTDKD
160 170 180 190 200
KALRSMYRVL KPGGRLLVLE FSKPIIEPLS KAYDAYSFHV LPRIGSLVAN
210 220 230 240 250
DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA GVVALHRGYK
F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87049 Genomic DNA Translation: AAA67628.1 U00096 Genomic DNA Translation: AAT48227.1 AP009048 Genomic DNA Translation: BAE77468.1 |
PIRi | B65188 |
RefSeqi | WP_000227958.1, NZ_STEB01000021.1 YP_026269.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48227; AAT48227; b3833 BAE77468; BAE77468; BAE77468 |
GeneIDi | 58389515 948926 |
KEGGi | ecj:JW5581 eco:b3833 |
PATRICi | fig|1411691.4.peg.2875 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87049 Genomic DNA Translation: AAA67628.1 U00096 Genomic DNA Translation: AAT48227.1 AP009048 Genomic DNA Translation: BAE77468.1 |
PIRi | B65188 |
RefSeqi | WP_000227958.1, NZ_STEB01000021.1 YP_026269.1, NC_000913.3 |
3D structure databases
SMRi | P0A887 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261082, 536 interactors 853202, 2 interactors |
DIPi | DIP-31831N |
IntActi | P0A887, 6 interactors |
STRINGi | 511145.b3833 |
Chemistry databases
SwissLipidsi | SLP:000001505 |
Proteomic databases
jPOSTi | P0A887 |
PaxDbi | P0A887 |
PRIDEi | P0A887 |
Genome annotation databases
EnsemblBacteriai | AAT48227; AAT48227; b3833 BAE77468; BAE77468; BAE77468 |
GeneIDi | 58389515 948926 |
KEGGi | ecj:JW5581 eco:b3833 |
PATRICi | fig|1411691.4.peg.2875 |
Organism-specific databases
EchoBASEi | EB1441 |
Phylogenomic databases
eggNOGi | COG2226, Bacteria |
HOGENOMi | CLU_037990_0_0_6 |
InParanoidi | P0A887 |
PhylomeDBi | P0A887 |
Enzyme and pathway databases
UniPathwayi | UPA00079;UER00169 UPA00232 |
BioCyci | EcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER |
BRENDAi | 2.1.1.163, 2026 |
Miscellaneous databases
PROi | PR:P0A887 |
Family and domain databases
HAMAPi | MF_01813, MenG_UbiE_methyltr, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR004033, UbiE/COQ5_MeTrFase IPR023576, UbiE/COQ5_MeTrFase_CS |
Pfami | View protein in Pfam PF01209, Ubie_methyltran, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR01934, MenG_MenH_UbiE, 1 hit |
PROSITEi | View protein in PROSITE PS51608, SAM_MT_UBIE, 1 hit PS01183, UBIE_1, 1 hit PS01184, UBIE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBIE_ECOLI | |
Accessioni | P0A887Primary (citable) accession number: P0A887 Secondary accession number(s): P27851, Q2M8D8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 10, 2021 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families