UniProtKB - P0A877 (TRPA_ECOLI)
Protein
Tryptophan synthase alpha chain
Gene
trpA
Organism
Escherichia coli (strain K12)
Status
Functioni
The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
Catalytic activityi
- (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophanUniRule annotationEC:4.2.1.20UniRule annotation
: L-tryptophan biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Bifunctional protein TrpGD (trpGD)
- Anthranilate phosphoribosyltransferase (trpD), Bifunctional protein TrpGD (trpGD)
- Multifunctional fusion protein (trpCF), Tryptophan biosynthesis protein TrpCF (trpC)
- Multifunctional fusion protein (trpCF), Tryptophan biosynthesis protein TrpCF (trpC)
- Tryptophan synthase alpha chain (trpA), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase beta chain (trpB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | Proton acceptorUniRule annotation | 1 | |
Active sitei | 60 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- lyase activity Source: EcoCyc
- tryptophan synthase activity Source: UniProtKB-UniRule
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: EcoliWiki
- tryptophan biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:TRYPSYN-APROTEIN MetaCyc:TRYPSYN-APROTEIN |
BRENDAi | 4.2.1.20, 2026 |
SABIO-RKi | P0A877 |
UniPathwayi | UPA00035;UER00044 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:trpAUniRule annotation Ordered Locus Names:b1260, JW1252 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098778 | 1 – 268 | Tryptophan synthase alpha chainAdd BLAST | 268 |
Proteomic databases
jPOSTi | P0A877 |
PaxDbi | P0A877 |
PRIDEi | P0A877 |
2D gel databases
SWISS-2DPAGEi | P0A877 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta chains.
Protein-protein interaction databases
BioGRIDi | 4260129, 34 interactors |
ComplexPortali | CPX-3446, TrpAB tryptophan synthase complex |
DIPi | DIP-35957N |
IntActi | P0A877, 6 interactors |
STRINGi | 511145.b1260 |
Chemistry databases
BindingDBi | P0A877 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A877 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A877 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TrpA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0159, Bacteria |
HOGENOMi | CLU_016734_0_4_6 |
InParanoidi | P0A877 |
PhylomeDBi | P0A877 |
Family and domain databases
CDDi | cd04724, Tryptophan_synthase_alpha, 1 hit |
DisProti | DP00252 |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00131, Trp_synth_alpha, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011060, RibuloseP-bd_barrel IPR018204, Trp_synthase_alpha_AS IPR002028, Trp_synthase_suA |
PANTHERi | PTHR43406, PTHR43406, 1 hit |
Pfami | View protein in Pfam PF00290, Trp_syntA, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00262, trpA, 1 hit |
PROSITEi | View protein in PROSITE PS00167, TRP_SYNTHASE_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A877-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL
60 70 80 90 100
GIPFSDPLAD GPTIQNATLR AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL
110 120 130 140 150
MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR QAALRHNVAP
160 170 180 190 200
IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL
210 220 230 240 250
KEYNAAPPLQ GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK
260
MLAALKVFVQ PMKAATRS
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 209 – 224 | LQGFG…VKAAI → IAGFWYFRPGSGKSSD in mutant TrpA46-Asp-PR3. Add BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00364 Genomic DNA Translation: CAA23662.1 V00372 Genomic DNA Translation: CAA23675.1 J01714 Genomic DNA Translation: AAA57301.1 U23489 Genomic DNA Translation: AAB60035.1 U23490 Genomic DNA Translation: AAA65140.1 U23491 Genomic DNA Translation: AAA65146.1 U23492 Genomic DNA Translation: AAA65152.1 U23494 Genomic DNA Translation: AAA65164.1 U25417 Genomic DNA Translation: AAA73791.1 U25418 Genomic DNA Translation: AAA73797.1 U25419 Genomic DNA Translation: AAA73803.1 U25420 Genomic DNA Translation: AAA73809.1 U25421 Genomic DNA Translation: AAA73815.1 U25422 Genomic DNA Translation: AAA73821.1 U00096 Genomic DNA Translation: AAC74342.1 AP009048 Genomic DNA Translation: BAA14792.1 X16698 Genomic DNA Translation: CAA34671.1 |
PIRi | E93746, TSECA |
RefSeqi | NP_415776.1, NC_000913.3 WP_000443067.1, NZ_SSZK01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC74342; AAC74342; b1260 BAA14792; BAA14792; BAA14792 |
GeneIDi | 58388661 946204 |
KEGGi | ecj:JW1252 eco:b1260 |
PATRICi | fig|1411691.4.peg.1023 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00364 Genomic DNA Translation: CAA23662.1 V00372 Genomic DNA Translation: CAA23675.1 J01714 Genomic DNA Translation: AAA57301.1 U23489 Genomic DNA Translation: AAB60035.1 U23490 Genomic DNA Translation: AAA65140.1 U23491 Genomic DNA Translation: AAA65146.1 U23492 Genomic DNA Translation: AAA65152.1 U23494 Genomic DNA Translation: AAA65164.1 U25417 Genomic DNA Translation: AAA73791.1 U25418 Genomic DNA Translation: AAA73797.1 U25419 Genomic DNA Translation: AAA73803.1 U25420 Genomic DNA Translation: AAA73809.1 U25421 Genomic DNA Translation: AAA73815.1 U25422 Genomic DNA Translation: AAA73821.1 U00096 Genomic DNA Translation: AAC74342.1 AP009048 Genomic DNA Translation: BAA14792.1 X16698 Genomic DNA Translation: CAA34671.1 |
PIRi | E93746, TSECA |
RefSeqi | NP_415776.1, NC_000913.3 WP_000443067.1, NZ_SSZK01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V7Y | X-ray | 2.50 | A/B | 1-268 | [»] | |
1WQ5 | X-ray | 2.30 | A/B | 1-268 | [»] | |
1XC4 | X-ray | 2.80 | A/B | 1-268 | [»] | |
1XCF | X-ray | 1.80 | A/B | 1-268 | [»] | |
SMRi | P0A877 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260129, 34 interactors |
ComplexPortali | CPX-3446, TrpAB tryptophan synthase complex |
DIPi | DIP-35957N |
IntActi | P0A877, 6 interactors |
STRINGi | 511145.b1260 |
Chemistry databases
BindingDBi | P0A877 |
ChEMBLi | CHEMBL3885646 |
2D gel databases
SWISS-2DPAGEi | P0A877 |
Proteomic databases
jPOSTi | P0A877 |
PaxDbi | P0A877 |
PRIDEi | P0A877 |
Genome annotation databases
EnsemblBacteriai | AAC74342; AAC74342; b1260 BAA14792; BAA14792; BAA14792 |
GeneIDi | 58388661 946204 |
KEGGi | ecj:JW1252 eco:b1260 |
PATRICi | fig|1411691.4.peg.1023 |
Organism-specific databases
EchoBASEi | EB1017 |
Phylogenomic databases
eggNOGi | COG0159, Bacteria |
HOGENOMi | CLU_016734_0_4_6 |
InParanoidi | P0A877 |
PhylomeDBi | P0A877 |
Enzyme and pathway databases
UniPathwayi | UPA00035;UER00044 |
BioCyci | EcoCyc:TRYPSYN-APROTEIN MetaCyc:TRYPSYN-APROTEIN |
BRENDAi | 4.2.1.20, 2026 |
SABIO-RKi | P0A877 |
Miscellaneous databases
EvolutionaryTracei | P0A877 |
PROi | PR:P0A877 |
Family and domain databases
CDDi | cd04724, Tryptophan_synthase_alpha, 1 hit |
DisProti | DP00252 |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00131, Trp_synth_alpha, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011060, RibuloseP-bd_barrel IPR018204, Trp_synthase_alpha_AS IPR002028, Trp_synthase_suA |
PANTHERi | PTHR43406, PTHR43406, 1 hit |
Pfami | View protein in Pfam PF00290, Trp_syntA, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00262, trpA, 1 hit |
PROSITEi | View protein in PROSITE PS00167, TRP_SYNTHASE_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRPA_ECOLI | |
Accessioni | P0A877Primary (citable) accession number: P0A877 Secondary accession number(s): P00928, Q47669 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families