UniProtKB - P0A877 (TRPA_ECOLI)

BLAST

>sp|P0A877|TRPA_ECOLI Tryptophan synthase alpha chain OS=Escherichia coli (strain K12) OX=83333 GN=trpA PE=1 SV=1
MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLAD
GPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEK
VGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRA
GVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKI
IEQHINEPEKMLAALKVFVQPMKAATRS

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History
Entry version 108 (07 Nov 2018)
Sequence version 1 (21 Jul 1986)
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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityⁱ

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H₂O.UniRule annotation

Pathwayⁱ: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
Bifunctional protein TrpGD (trpGD)
Tryptophan biosynthesis protein TrpCF (trpC)
Tryptophan biosynthesis protein TrpCF (trpC)
Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)

This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	49	Proton acceptorUniRule annotation		1
Active siteⁱ	60	Proton acceptorUniRule annotation		1

GO - Molecular functionⁱ

lyase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 1899027
tryptophan synthase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aromatic amino acid family biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 8463331
tryptophan biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 8463331

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:TRYPSYN-APROTEIN MetaCyc:TRYPSYN-APROTEIN
BRENDAⁱ	4.2.1.20 2026
SABIO-RKⁱ	P0A877
UniPathwayⁱ	UPA00035;UER00044

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesⁱ	Name:trpAUniRule annotation Ordered Locus Names:b1260, JW1252
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11024 trpA

EcoGeneⁱ

EG11024 trpA

Subcellular locationⁱ

GO - Cellular componentⁱ

cytoplasm
Source: EcoliWiki
Inferred from direct assayⁱ
- 4887511
cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3885646

ChEMBLⁱ

CHEMBL3885646

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000098778	1 – 268	Tryptophan synthase alpha chainAdd BLAST		268

Proteomic databases

EPDⁱ	P0A877
PaxDbⁱ	P0A877
PRIDEⁱ	P0A877

2D gel databases

SWISS-2DPAGEⁱ	P0A877

SWISS-2DPAGEⁱ

P0A877

Interactionⁱ

Subunit structureⁱ

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

BioGridⁱ	4260129, 34 interactors
ComplexPortalⁱ	CPX-3446 TrpAB tryptophan synthase complex
Database of interacting proteins More...DIPⁱ	DIP-35957N
IntActⁱ	P0A877, 6 interactors
STRINGⁱ	316385.ECDH10B_1375

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 12	Combined sources	10
Turnⁱ	13 – 15	Combined sources	3
Beta strandⁱ	18 – 24	Combined sources	7
Beta strandⁱ	26 – 28	Combined sources	3
Helixⁱ	30 – 43	Combined sources	14
Beta strandⁱ	46 – 51	Combined sources	6
Helixⁱ	70 – 73	Combined sources	4
Helixⁱ	78 – 91	Combined sources	14
Beta strandⁱ	93 – 95	Combined sources	3
Beta strandⁱ	97 – 101	Combined sources	5
Helixⁱ	103 – 109	Combined sources	7
Helixⁱ	111 – 121	Combined sources	11
Beta strandⁱ	125 – 128	Combined sources	4
Helixⁱ	133 – 135	Combined sources	3
Helixⁱ	137 – 145	Combined sources	9
Beta strandⁱ	149 – 151	Combined sources	3
Helixⁱ	160 – 169	Combined sources	10
Beta strandⁱ	174 – 176	Combined sources	3
Helixⁱ	179 – 181	Combined sources	3
Helixⁱ	193 – 202	Combined sources	10
Beta strandⁱ	208 – 211	Combined sources	4
Helixⁱ	217 – 225	Combined sources	9
Beta strandⁱ	229 – 233	Combined sources	5
Helixⁱ	236 – 242	Combined sources	7
Turnⁱ	244 – 246	Combined sources	3
Helixⁱ	248 – 264	Combined sources	17

Show more details

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00252
ProteinModelPortalⁱ	P0A877
SMRⁱ	P0A877
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0A877

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105F6H Bacteria COG0159 LUCA
HOGENOMⁱ	HOG000223814
InParanoidⁱ	P0A877
KEGG Orthology (KO) More...KOⁱ	K01695
PhylomeDBⁱ	P0A877

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_00131 Trp_synth_alpha, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR011060 RibuloseP-bd_barrel IPR018204 Trp_synthase_alpha_AS IPR002028 Trp_synthase_suA
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00290 Trp_syntA, 1 hit
SUPFAMⁱ	SSF51366 SSF51366, 1 hit
TIGRFAMsⁱ	TIGR00262 trpA, 1 hit
PROSITEⁱ	View protein in PROSITE PS00167 TRP_SYNTHASE_ALPHA, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0A877-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL 
        60         70         80         90        100
GIPFSDPLAD GPTIQNATLR AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL 
       110        120        130        140        150
MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR QAALRHNVAP 
       160        170        180        190        200
IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL 
       210        220        230        240        250
KEYNAAPPLQ GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK 
       260 
MLAALKVFVQ PMKAATRS

Length:268

Mass (Da):28,724

Last modified:July 21, 1986 - v1

Checksum:ⁱ028223DB2B666CC6

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	209 – 224	LQGFG…VKAAI → IAGFWYFRPGSGKSSD in mutant TrpA46-Asp-PR3. Add BLAST		16

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00364 Genomic DNA Translation: CAA23662.1 V00372 Genomic DNA Translation: CAA23675.1 J01714 Genomic DNA Translation: AAA57301.1 U23489 Genomic DNA Translation: AAB60035.1 U23490 Genomic DNA Translation: AAA65140.1 U23491 Genomic DNA Translation: AAA65146.1 U23492 Genomic DNA Translation: AAA65152.1 U23494 Genomic DNA Translation: AAA65164.1 U25417 Genomic DNA Translation: AAA73791.1 U25418 Genomic DNA Translation: AAA73797.1 U25419 Genomic DNA Translation: AAA73803.1 U25420 Genomic DNA Translation: AAA73809.1 U25421 Genomic DNA Translation: AAA73815.1 U25422 Genomic DNA Translation: AAA73821.1 U00096 Genomic DNA Translation: AAC74342.1 AP009048 Genomic DNA Translation: BAA14792.1 X16698 Genomic DNA Translation: CAA34671.1
PIRⁱ	E93746 TSECA
NCBI Reference Sequences More...RefSeqⁱ	NP_415776.1, NC_000913.3 WP_000443067.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74342; AAC74342; b1260 BAA14792; BAA14792; BAA14792
GeneIDⁱ	946204
KEGGⁱ	ecj:JW1252 eco:b1260
PATRICⁱ	fig\|1411691.4.peg.1023

Protein	Similar proteins		Species	Score	Length	Source
P0A877	Tryptophan synthase alpha chain		ECOBW		268	UniRef100_C4ZTV3
Tryptophan synthase alpha chain		ECODH		268
Tryptophan synthase alpha chain		ECOHS		268
Tryptophan synthase alpha chain		ECOLC		268
Tryptophan synthase alpha chain		SHIFL		268
+54

Protein	Similar proteins		Species	Score	Length	Source
P0A877	Tryptophan synthase alpha chain		SHIDS		268	UniRef90_Q32GT0
Tryptophan synthase alpha chain		ECOBW		268
Tryptophan synthase alpha chain		ECODH		268
Tryptophan synthase alpha chain		ECOHS		268
Tryptophan synthase alpha chain		ECOLC		268
+756

Protein	Similar proteins		Species	Score	Length	Source
P0A877	Tryptophan synthase alpha chain	)	SALTY		268	UniRef50_P00929
Tryptophan synthase alpha chain		SALAR		269
Tryptophan synthase alpha chain		SALSV		268
Tryptophan synthase alpha chain		SALCH		268
Tryptophan synthase alpha chain		SALPC		268
+3828

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00364 Genomic DNA Translation: CAA23662.1 V00372 Genomic DNA Translation: CAA23675.1 J01714 Genomic DNA Translation: AAA57301.1 U23489 Genomic DNA Translation: AAB60035.1 U23490 Genomic DNA Translation: AAA65140.1 U23491 Genomic DNA Translation: AAA65146.1 U23492 Genomic DNA Translation: AAA65152.1 U23494 Genomic DNA Translation: AAA65164.1 U25417 Genomic DNA Translation: AAA73791.1 U25418 Genomic DNA Translation: AAA73797.1 U25419 Genomic DNA Translation: AAA73803.1 U25420 Genomic DNA Translation: AAA73809.1 U25421 Genomic DNA Translation: AAA73815.1 U25422 Genomic DNA Translation: AAA73821.1 U00096 Genomic DNA Translation: AAC74342.1 AP009048 Genomic DNA Translation: BAA14792.1 X16698 Genomic DNA Translation: CAA34671.1
PIRⁱ	E93746 TSECA
RefSeqⁱ	NP_415776.1, NC_000913.3 WP_000443067.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1V7Y	X-ray	2.50	A/B	1-268	[»]
	1WQ5	X-ray	2.30	A/B	1-268	[»]
	1XC4	X-ray	2.80	A/B	1-268	[»]
	1XCF	X-ray	1.80	A/B	1-268	[»]
DisProtⁱ	DP00252
ProteinModelPortalⁱ	P0A877
SMRⁱ	P0A877
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260129, 34 interactors
ComplexPortalⁱ	CPX-3446 TrpAB tryptophan synthase complex
DIPⁱ	DIP-35957N
IntActⁱ	P0A877, 6 interactors
STRINGⁱ	316385.ECDH10B_1375

Chemistry databases

ChEMBLⁱ	CHEMBL3885646

ChEMBLⁱ

CHEMBL3885646

2D gel databases

SWISS-2DPAGEⁱ	P0A877

SWISS-2DPAGEⁱ

P0A877

Proteomic databases

EPDⁱ	P0A877
PaxDbⁱ	P0A877
PRIDEⁱ	P0A877

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74342; AAC74342; b1260 BAA14792; BAA14792; BAA14792
GeneIDⁱ	946204
KEGGⁱ	ecj:JW1252 eco:b1260
PATRICⁱ	fig\|1411691.4.peg.1023

Organism-specific databases

EchoBASEⁱ	EB1017
EcoGeneⁱ	EG11024 trpA

Phylogenomic databases

eggNOGⁱ	ENOG4105F6H Bacteria COG0159 LUCA
HOGENOMⁱ	HOG000223814
InParanoidⁱ	P0A877
KOⁱ	K01695
PhylomeDBⁱ	P0A877

Enzyme and pathway databases

UniPathwayⁱ	UPA00035;UER00044
BioCycⁱ	EcoCyc:TRYPSYN-APROTEIN MetaCyc:TRYPSYN-APROTEIN
BRENDAⁱ	4.2.1.20 2026
SABIO-RKⁱ	P0A877

Miscellaneous databases

EvolutionaryTraceⁱ	P0A877
Protein Ontology More...PROⁱ	PR:P0A877

Family and domain databases

CDDⁱ	cd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_00131 Trp_synth_alpha, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR011060 RibuloseP-bd_barrel IPR018204 Trp_synthase_alpha_AS IPR002028 Trp_synthase_suA
Pfamⁱ	View protein in Pfam PF00290 Trp_syntA, 1 hit
SUPFAMⁱ	SSF51366 SSF51366, 1 hit
TIGRFAMsⁱ	TIGR00262 trpA, 1 hit
PROSITEⁱ	View protein in PROSITE PS00167 TRP_SYNTHASE_ALPHA, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRPA_ECOLI
Accessionⁱ	P0A877Primary (citable) accession number: P0A877 Secondary accession number(s): P00928, Q47669
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	July 21, 1986
	Last modified:	November 7, 2018
	This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A877 (TRPA_ECOLI)

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Tryptophan synthase alpha chain

trpA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-tryptophan biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: L-tryptophan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ