UniProtKB - P0A870 (TALB_ECOLI)
Protein
Transaldolase B
Gene
talB
Organism
Escherichia coli (strain K12)
Status
Functioni
Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.1 Publication
Catalytic activityi
- D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = β-D-fructose 6-phosphate + D-erythrose 4-phosphate1 PublicationEC:2.2.1.21 Publication
Activity regulationi
Inhibited by Tris-HCl and phosphate buffer. Also competitively inhibited by sugars with L configuration at C2, such as D-arabinose-5-phosphate and L-glyceraldehyde.1 Publication
Kineticsi
- KM=38 µM for D,L-glyceraldehyde 3-phosphate (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
- KM=90 µM for D-erythrose-4-phosphate (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
- KM=31 mM for D-ribose-5-phosphate (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
- KM=28 mM for D-glyceraldehyde (with C3 acceptor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
- KM=285 µM for D-sedoheptulose-7-phosphate (with C3 donor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
- KM=1200 µM for D-fructose-6-phosphate (with C3 donor compounds at pH 8.5 and 30 degrees Celsius)1 Publication
pH dependencei
Optimum pH is between 8.5 and 9.5.1 Publication
Temperature dependencei
Optimum temperature is between 15 and 40 degrees Celsius. At temperatures above 50 degrees Celsius, activity is lost rapidly, and at 55 degrees Celsius, the enzyme is totally inactivated.1 Publication
: pentose phosphate pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage).Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Transaldolase (tal), Transaldolase (tal), Transaldolase A (talA), Transaldolase B (talB)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 132 | Schiff-base intermediate with substrate1 Publication | 1 |
GO - Molecular functioni
- sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glyceronetransferase activity Source: UniProtKB
- transferase activity, transferring aldehyde or ketonic groups Source: EcoCyc
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- pentose-phosphate shunt, non-oxidative branch Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Pentose shunt |
Ligand | Schiff base |
Enzyme and pathway databases
BioCyci | EcoCyc:TRANSALDOLB-MONOMER MetaCyc:TRANSALDOLB-MONOMER |
BRENDAi | 2.2.1.2, 2026 |
UniPathwayi | UPA00115;UER00414 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:talB Synonyms:yaaK Ordered Locus Names:b0008, JW0007 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000173593 | 2 – 317 | Transaldolase BAdd BLAST | 316 |
Proteomic databases
jPOSTi | P0A870 |
PaxDbi | P0A870 |
PRIDEi | P0A870 |
2D gel databases
SWISS-2DPAGEi | P0A870 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261939, 9 interactors 849151, 1 interactor |
DIPi | DIP-31850N |
IntActi | P0A870, 9 interactors |
STRINGi | 511145.b0008 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A870 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A870 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0176, Bacteria |
HOGENOMi | CLU_047470_0_1_6 |
InParanoidi | P0A870 |
PhylomeDBi | P0A870 |
Family and domain databases
CDDi | cd00957, Transaldolase_TalAB, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00492, Transaldolase_1, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001585, TAL/FSA IPR004730, Transaldolase_1 IPR018225, Transaldolase_AS |
PANTHERi | PTHR10683, PTHR10683, 1 hit PTHR10683:SF18, PTHR10683:SF18, 1 hit |
Pfami | View protein in Pfam PF00923, TAL_FSA, 1 hit |
TIGRFAMsi | TIGR00874, talAB, 1 hit |
PROSITEi | View protein in PROSITE PS01054, TRANSALDOLASE_1, 1 hit PS00958, TRANSALDOLASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A870-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDKLTSLRQ YTTVVADTGD IAAMKLYQPQ DATTNPSLIL NAAQIPEYRK
60 70 80 90 100
LIDDAVAWAK QQSNDRAQQI VDATDKLAVN IGLEILKLVP GRISTEVDAR
110 120 130 140 150
LSYDTEASIA KAKRLIKLYN DAGISNDRIL IKLASTWQGI RAAEQLEKEG
160 170 180 190 200
INCNLTLLFS FAQARACAEA GVFLISPFVG RILDWYKANT DKKEYAPAED
210 220 230 240 250
PGVVSVSEIY QYYKEHGYET VVMGASFRNI GEILELAGCD RLTIAPALLK
260 270 280 290 300
ELAESEGAIE RKLSYTGEVK ARPARITESE FLWQHNQDPM AVDKLAEGIR
310
KFAIDQEKLE KMIGDLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13161 Genomic DNA Translation: BAA21822.1 S80045 Genomic DNA Translation: AAB47022.1 U00096 Genomic DNA Translation: AAC73119.1 AP009048 Genomic DNA Translation: BAB96586.1 |
PIRi | S40535 |
RefSeqi | NP_414549.1, NC_000913.3 WP_000130185.1, NZ_STEB01000033.1 |
Genome annotation databases
EnsemblBacteriai | AAC73119; AAC73119; b0008 BAB96586; BAB96586; BAB96586 |
GeneIDi | 58461421 944748 |
KEGGi | ecj:JW0007 eco:b0008 |
PATRICi | fig|1411691.4.peg.2275 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Transaldolase B entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13161 Genomic DNA Translation: BAA21822.1 S80045 Genomic DNA Translation: AAB47022.1 U00096 Genomic DNA Translation: AAC73119.1 AP009048 Genomic DNA Translation: BAB96586.1 |
PIRi | S40535 |
RefSeqi | NP_414549.1, NC_000913.3 WP_000130185.1, NZ_STEB01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I2N | X-ray | 2.05 | A/B | 2-317 | [»] | |
1I2O | X-ray | 2.05 | A/B | 2-317 | [»] | |
1I2P | X-ray | 2.05 | A/B | 2-317 | [»] | |
1I2Q | X-ray | 2.05 | A/B | 2-317 | [»] | |
1I2R | X-ray | 2.10 | A/B | 2-317 | [»] | |
1ONR | X-ray | 1.87 | A/B | 2-317 | [»] | |
1UCW | X-ray | 2.20 | A/B | 1-317 | [»] | |
3KOF | X-ray | 1.90 | A/B | 1-317 | [»] | |
4RZ5 | X-ray | 1.80 | A/B | 1-317 | [»] | |
4RZ6 | X-ray | 1.80 | A/B | 1-317 | [»] | |
4S2B | X-ray | 1.46 | A/B | 1-317 | [»] | |
4S2C | X-ray | 2.20 | A/B | 1-317 | [»] | |
SMRi | P0A870 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261939, 9 interactors 849151, 1 interactor |
DIPi | DIP-31850N |
IntActi | P0A870, 9 interactors |
STRINGi | 511145.b0008 |
2D gel databases
SWISS-2DPAGEi | P0A870 |
Proteomic databases
jPOSTi | P0A870 |
PaxDbi | P0A870 |
PRIDEi | P0A870 |
Genome annotation databases
EnsemblBacteriai | AAC73119; AAC73119; b0008 BAB96586; BAB96586; BAB96586 |
GeneIDi | 58461421 944748 |
KEGGi | ecj:JW0007 eco:b0008 |
PATRICi | fig|1411691.4.peg.2275 |
Organism-specific databases
EchoBASEi | EB1517 |
Phylogenomic databases
eggNOGi | COG0176, Bacteria |
HOGENOMi | CLU_047470_0_1_6 |
InParanoidi | P0A870 |
PhylomeDBi | P0A870 |
Enzyme and pathway databases
UniPathwayi | UPA00115;UER00414 |
BioCyci | EcoCyc:TRANSALDOLB-MONOMER MetaCyc:TRANSALDOLB-MONOMER |
BRENDAi | 2.2.1.2, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A870 |
PROi | PR:P0A870 |
Family and domain databases
CDDi | cd00957, Transaldolase_TalAB, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00492, Transaldolase_1, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001585, TAL/FSA IPR004730, Transaldolase_1 IPR018225, Transaldolase_AS |
PANTHERi | PTHR10683, PTHR10683, 1 hit PTHR10683:SF18, PTHR10683:SF18, 1 hit |
Pfami | View protein in Pfam PF00923, TAL_FSA, 1 hit |
TIGRFAMsi | TIGR00874, talAB, 1 hit |
PROSITEi | View protein in PROSITE PS01054, TRANSALDOLASE_1, 1 hit PS00958, TRANSALDOLASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TALB_ECOLI | |
Accessioni | P0A870Primary (citable) accession number: P0A870 Secondary accession number(s): P30148 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families