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UniProtKB - P0A858 (TPIS_ECOLI)
Protein
Triosephosphate isomerase
Gene
tpiA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).
UniRule annotation1 PublicationCatalytic activityi
- EC:5.3.1.1UniRule annotation2 Publications
Kineticsi
kcat is 54000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at 25 degrees Celsius).1 Publication
- KM=1030 µM for D-glyceraldehyde 3-phosphate (at 25 degrees Celsius)1 Publication
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Pathwayi: glycolysis
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 95 | ElectrophileUniRule annotation | 1 | |
Active sitei | 167 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 173 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 212 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- triose-phosphate isomerase activity Source: EcoCyc
GO - Biological processi
- gluconeogenesis Source: EcoliWiki
- glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
- glycerol catabolic process Source: GO_Central
- glycolytic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
BioCyci | EcoCyc:TPI-MONOMER |
BRENDAi | 5.3.1.1, 2026 |
SABIO-RKi | P0A858 |
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: Triosephosphate isomerase1 PublicationUniRule annotation (EC:5.3.1.1UniRule annotation2 Publications)Short name: TIM1 PublicationUniRule annotation Short name: TPI1 PublicationUniRule annotation Alternative name(s): Triose-phosphate isomeraseUniRule annotation |
Gene namesi | Name:tpiA1 PublicationUniRule annotation Synonyms:tpi Ordered Locus Names:b3919, JW3890 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotationCurated
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene accumulates methylglyoxal and are unable to grow on glucose, lactate or other carbon sources.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090217 | 1 – 255 | Triosephosphate isomeraseAdd BLAST | 255 |
Proteomic databases
jPOSTi | P0A858 |
PaxDbi | P0A858 |
PRIDEi | P0A858 |
2D gel databases
SWISS-2DPAGEi | P0A858 |
Expressioni
Inductioni
Induced by CsrA and repressed by spermidine.2 Publications
Interactioni
Subunit structurei
Homodimer.
UniRule annotation3 PublicationsBinary interactionsi
P0A858
With | #Exp. | IntAct |
---|---|---|
pepT [P29745] | 7 | EBI-368978,EBI-555639 |
pflB [P09373] | 7 | EBI-368978,EBI-546682 |
itself | 3 | EBI-368978,EBI-368978 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262647, 12 interactors 852706, 2 interactors |
DIPi | DIP-31849N |
IntActi | P0A858, 28 interactors |
STRINGi | 511145.b3919 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A858 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A858 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 11 | Substrate bindingUniRule annotation | 3 | |
Regioni | 233 – 234 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0149, Bacteria |
HOGENOMi | CLU_024251_2_1_6 |
InParanoidi | P0A858 |
PhylomeDBi | P0A858 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A858-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRHPLVMGNW KLNGSRHMVH ELVSNLRKEL AGVAGCAVAI APPEMYIDMA
60 70 80 90 100
KREAEGSHIM LGAQNVDLNL SGAFTGETSA AMLKDIGAQY IIIGHSERRT
110 120 130 140 150
YHKESDELIA KKFAVLKEQG LTPVLCIGET EAENEAGKTE EVCARQIDAV
160 170 180 190 200
LKTQGAAAFE GAVIAYEPVW AIGTGKSATP AQAQAVHKFI RDHIAKVDAN
210 220 230 240 250
IAEQVIIQYG GSVNASNAAE LFAQPDIDGA LVGGASLKAD AFAVIVKAAE
AAKQA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | D → N in CAA25253 (PubMed:6092857).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00617 Genomic DNA Translation: CAA25253.1 L19201 Genomic DNA Translation: AAB03051.1 U00096 Genomic DNA Translation: AAC76901.1 AP009048 Genomic DNA Translation: BAE77391.1 |
PIRi | B65198, ISECT |
RefSeqi | NP_418354.1, NC_000913.3 WP_001216325.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76901; AAC76901; b3919 BAE77391; BAE77391; BAE77391 |
GeneIDi | 66672173 948409 |
KEGGi | ecj:JW3890 eco:b3919 |
PATRICi | fig|1411691.4.peg.2786 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00617 Genomic DNA Translation: CAA25253.1 L19201 Genomic DNA Translation: AAB03051.1 U00096 Genomic DNA Translation: AAC76901.1 AP009048 Genomic DNA Translation: BAE77391.1 |
PIRi | B65198, ISECT |
RefSeqi | NP_418354.1, NC_000913.3 WP_001216325.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TMH | X-ray | 2.80 | A/B/C/D | 1-255 | [»] | |
1TRE | X-ray | 2.60 | A/B | 1-255 | [»] | |
SMRi | P0A858 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262647, 12 interactors 852706, 2 interactors |
DIPi | DIP-31849N |
IntActi | P0A858, 28 interactors |
STRINGi | 511145.b3919 |
2D gel databases
SWISS-2DPAGEi | P0A858 |
Proteomic databases
jPOSTi | P0A858 |
PaxDbi | P0A858 |
PRIDEi | P0A858 |
Genome annotation databases
EnsemblBacteriai | AAC76901; AAC76901; b3919 BAE77391; BAE77391; BAE77391 |
GeneIDi | 66672173 948409 |
KEGGi | ecj:JW3890 eco:b3919 |
PATRICi | fig|1411691.4.peg.2786 |
Organism-specific databases
EchoBASEi | EB1008 |
Phylogenomic databases
eggNOGi | COG0149, Bacteria |
HOGENOMi | CLU_024251_2_1_6 |
InParanoidi | P0A858 |
PhylomeDBi | P0A858 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
BioCyci | EcoCyc:TPI-MONOMER |
BRENDAi | 5.3.1.1, 2026 |
SABIO-RKi | P0A858 |
Miscellaneous databases
EvolutionaryTracei | P0A858 |
PROi | PR:P0A858 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_ECOLI | |
Accessioni | P0A858Primary (citable) accession number: P0A858 Secondary accession number(s): P04790, Q2M8L5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families