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Protein

Tol-Pal system protein TolB

Gene

tolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:17233825). TolB occupies a key intermediary position in the Tol-Pal system because it communicates directly with both membrane-embedded components, Pal in the outer membrane and TolA in the inner membrane (PubMed:19696740). Is also involved in the uptake of some colicins A (PubMed:2687247, PubMed:7929011, PubMed:11994151). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).2 Publications4 Publications

GO - Molecular functioni

  • protein-containing complex binding Source: CAFA
  • protein domain specific binding Source: CAFA

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-UniRule
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • protein transport Source: EcoliWiki

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER
MetaCyc:EG11008-MONOMER

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Names & Taxonomyi

Protein namesi
Recommended name:
Tol-Pal system protein TolBUniRule annotationCurated
Gene namesi
Name:tolB1 PublicationUniRule annotation
Ordered Locus Names:b0740, JW5100
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11008 tolB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotation2 PublicationsAdd BLAST21
ChainiPRO_000003464722 – 430Tol-Pal system protein TolBUniRule annotationAdd BLAST409

Proteomic databases

EPDiP0A855
PaxDbiP0A855
PRIDEiP0A855

2D gel databases

SWISS-2DPAGEiP0A855

Interactioni

Subunit structurei

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). TolB forms homodimers (PubMed:11994151). Interacts with the peptidoglycan-associated lipoprotein Pal and with the C-terminal region of TolA (PubMed:7744736, PubMed:11994151, PubMed:17375930, PubMed:19696740). Interacts with the trimeric porins OmpF, OmpC, PhoE and LamB (PubMed:9393690). Can form a complex with Pal, Lpp and OmpA (PubMed:9701827). Interacts with colicin E9 (PubMed:10673426, PubMed:16894158).1 Publication8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261828, 397 interactors
DIPiDIP-48262N
IntActiP0A855, 11 interactors
MINTiP0A855
STRINGi316385.ECDH10B_0807

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A855
SMRiP0A855
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A855

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 34TolA box1 PublicationAdd BLAST13

Domaini

Effector proteins that bind to TolB can both negatively (Pal) and positively (colicin E9) regulate association of the distal N-terminal TolA box with TolA by influencing the conformational equilibrium experienced by these residues.1 Publication

Sequence similaritiesi

Belongs to the TolB family.UniRule annotationCurated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6Y Bacteria
COG0823 LUCA
HOGENOMiHOG000004098
InParanoidiP0A855
KOiK03641
PhylomeDBiP0A855

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.10070, 1 hit
HAMAPiMF_00671 TolB, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR011659 PD40
IPR014167 Tol-Pal_TolB
IPR007195 TolB_N
IPR036752 TolB_N_sf
PfamiView protein in Pfam
PF07676 PD40, 4 hits
PF04052 TolB_N, 1 hit
TIGRFAMsiTIGR02800 propeller_TolB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A855-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA
60 70 80 90 100
PEDIGGIVAA DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV
110 120 130 140 150
VGQVTPNPDG SYNVAYQLVD TGGAPGTVLA QNSYKVNKQW LRYAGHTASD
160 170 180 190 200
EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY ELRVSDYDGY NQFVVHRSPQ
210 220 230 240 250
PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA SFPRHNGAPA
260 270 280 290 300
FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ
310 320 330 340 350
NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS
360 370 380 390 400
SNGGQQHIAK QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV
410 420 430
LNLVSTDGRF KARLPATDGQ VKFPAWSPYL
Length:430
Mass (Da):45,956
Last modified:June 7, 2005 - v1
Checksum:i508795C6E6886B52
GO

Sequence cautioni

The sequence AAA24684 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA Translation: AAA24684.1 Different initiation.
U00096 Genomic DNA Translation: AAC73834.1
AP009048 Genomic DNA Translation: BAA35406.2
X65796 Genomic DNA Translation: CAA46672.1
PIRiC64810
RefSeqiNP_415268.1, NC_000913.3
WP_001295307.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740
BAA35406; BAA35406; BAA35406
GeneIDi945429
KEGGiecj:JW5100
eco:b0740
PATRICifig|1411691.4.peg.1532

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA Translation: AAA24684.1 Different initiation.
U00096 Genomic DNA Translation: AAC73834.1
AP009048 Genomic DNA Translation: BAA35406.2
X65796 Genomic DNA Translation: CAA46672.1
PIRiC64810
RefSeqiNP_415268.1, NC_000913.3
WP_001295307.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855
SMRiP0A855
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261828, 397 interactors
DIPiDIP-48262N
IntActiP0A855, 11 interactors
MINTiP0A855
STRINGi316385.ECDH10B_0807

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

2D gel databases

SWISS-2DPAGEiP0A855

Proteomic databases

EPDiP0A855
PaxDbiP0A855
PRIDEiP0A855

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740
BAA35406; BAA35406; BAA35406
GeneIDi945429
KEGGiecj:JW5100
eco:b0740
PATRICifig|1411691.4.peg.1532

Organism-specific databases

EchoBASEiEB1001
EcoGeneiEG11008 tolB

Phylogenomic databases

eggNOGiENOG4107R6Y Bacteria
COG0823 LUCA
HOGENOMiHOG000004098
InParanoidiP0A855
KOiK03641
PhylomeDBiP0A855

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER
MetaCyc:EG11008-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A855
PROiPR:P0A855

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.10070, 1 hit
HAMAPiMF_00671 TolB, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR011659 PD40
IPR014167 Tol-Pal_TolB
IPR007195 TolB_N
IPR036752 TolB_N_sf
PfamiView protein in Pfam
PF07676 PD40, 4 hits
PF04052 TolB_N, 1 hit
TIGRFAMsiTIGR02800 propeller_TolB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTOLB_ECOLI
AccessioniPrimary (citable) accession number: P0A855
Secondary accession number(s): P19935
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 7, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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