UniProtKB - P0A847 (TGT_ECOLI)
Protein
Queuine tRNA-ribosyltransferase
Gene
tgt
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation2 Publications
Catalytic activityi
- 7-aminomethyl-7-carbaguanine + guanosine34 in tRNA = 7-aminomethyl-7-carbaguanosine34 in tRNA + guanineUniRule annotation2 PublicationsEC:2.4.2.29UniRule annotation2 Publications
Cofactori
Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication
Kineticsi
kcat is 0.00121 sec(-1) with tRNA(Tyr) and guanine as substrates.1 Publication
- KM=0.12 µM for tRNA(Tyr)1 Publication
- KM=0.1 µM for guanine1 Publication
: tRNA-queuosine biosynthesis Pathwayi
This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Proton acceptorUniRule annotation1 Publication | 1 | |
Binding sitei | 143 | SubstrateUniRule annotation | 1 | |
Binding sitei | 187 | SubstrateUniRule annotation | 1 | |
Binding sitei | 214 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Active sitei | 264 | NucleophileUniRule annotation1 Publication | 1 | |
Metal bindingi | 302 | ZincUniRule annotation1 Publication | 1 | |
Metal bindingi | 304 | ZincUniRule annotation1 Publication | 1 | |
Metal bindingi | 307 | ZincUniRule annotation1 Publication | 1 | |
Metal bindingi | 333 | Zinc; via pros nitrogenUniRule annotation | 1 |
GO - Molecular functioni
- queuine tRNA-ribosyltransferase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- queuosine biosynthetic process Source: EcoCyc
- tRNA-guanine transglycosylation Source: InterPro
- tRNA wobble guanine modification Source: EcoCyc
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Queuosine biosynthesis, tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10996-MONOMER MetaCyc:EG10996-MONOMER |
BRENDAi | 2.4.2.29, 2026 |
SABIO-RKi | P0A847 |
UniPathwayi | UPA00392 |
Names & Taxonomyi
Protein namesi | Recommended name: Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation2 Publications)Alternative name(s): Guanine insertion enzymeUniRule annotation tRNA-guanine transglycosylaseUniRule annotation |
Gene namesi | Name:tgtUniRule annotation Ordered Locus Names:b0406, JW0396 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 28 | C → A: Slight loss of activity. 1 Publication | 1 | |
Mutagenesisi | 89 | D → E: Reduces catalytic activity by 51%. 1 Publication | 1 | |
Mutagenesisi | 90 | S → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 90 | S → C: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 90 | S → F: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 145 | C → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 232 | C → A: Slight loss of activity. 1 Publication | 1 | |
Mutagenesisi | 264 | D → A, N, Q, K or H: Loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 264 | D → E: Reduces catalytic activity by 88%. 1 Publication | 1 | |
Mutagenesisi | 265 | C → A: Significant loss of activity. 1 Publication | 1 | |
Mutagenesisi | 302 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 304 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 307 | C → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 316 | H → A: Slight loss of activity. 1 Publication | 1 | |
Mutagenesisi | 317 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 321 | C → A: No loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135471 | 1 – 375 | Queuine tRNA-ribosyltransferaseAdd BLAST | 375 |
Proteomic databases
jPOSTi | P0A847 |
PaxDbi | P0A847 |
PRIDEi | P0A847 |
Interactioni
Subunit structurei
Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4263531, 21 interactors |
DIPi | DIP-36020N |
IntActi | P0A847, 39 interactors |
STRINGi | 511145.b0406 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 89 – 93 | Substrate bindingUniRule annotation | 5 | |
Regioni | 245 – 251 | RNA bindingUniRule annotation | 7 | |
Regioni | 269 – 273 | RNA binding; important for wobble base 34 recognitionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0343, Bacteria |
HOGENOMi | CLU_022060_0_1_6 |
InParanoidi | P0A847 |
PhylomeDBi | P0A847 |
Family and domain databases
Gene3Di | 3.20.20.105, 1 hit |
HAMAPi | MF_00168, Q_tRNA_Tgt, 1 hit |
InterProi | View protein in InterPro IPR004803, TGT IPR036511, TGT-like_sf IPR002616, tRNA_ribo_trans-like |
Pfami | View protein in Pfam PF01702, TGT, 1 hit |
SUPFAMi | SSF51713, SSF51713, 1 hit |
TIGRFAMsi | TIGR00430, Q_tRNA_tgt, 1 hit TIGR00449, tgt_general, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A847-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG
60 70 80 90 100
AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD
110 120 130 140 150
IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA
160 170 180 190 200
DWDYAKRSME MSLRWAKRSR ERFDSLGNKN ALFGIIQGSV YEDLRDISVK
210 220 230 240 250
GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE
260 270 280 290 300
DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP
310 320 330 340 350
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE
360 370
EGKLESFVTD FYQRQGREVP PLNVD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 – 55 | IL → MV (PubMed:1706703).Curated | 2 | |
Sequence conflicti | 54 – 55 | IL → MV (PubMed:8323579).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63939 Genomic DNA Translation: AAA24667.1 U00096 Genomic DNA Translation: AAC73509.1 AP009048 Genomic DNA Translation: BAE76186.1 U82664 Genomic DNA Translation: AAB40162.1 X56175 Genomic DNA Translation: CAA39631.1 Sequence problems. X56175 Genomic DNA Translation: CAA39632.1 Sequence problems. S68715 Genomic DNA Translation: AAC60467.2 |
PIRi | C38530 |
RefSeqi | NP_414940.1, NC_000913.3 WP_000667319.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73509; AAC73509; b0406 BAE76186; BAE76186; BAE76186 |
GeneIDi | 58460351 949130 |
KEGGi | ecj:JW0396 eco:b0406 |
PATRICi | fig|1411691.4.peg.1871 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63939 Genomic DNA Translation: AAA24667.1 U00096 Genomic DNA Translation: AAC73509.1 AP009048 Genomic DNA Translation: BAE76186.1 U82664 Genomic DNA Translation: AAB40162.1 X56175 Genomic DNA Translation: CAA39631.1 Sequence problems. X56175 Genomic DNA Translation: CAA39632.1 Sequence problems. S68715 Genomic DNA Translation: AAC60467.2 |
PIRi | C38530 |
RefSeqi | NP_414940.1, NC_000913.3 WP_000667319.1, NZ_STEB01000007.1 |
3D structure databases
SMRi | P0A847 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263531, 21 interactors |
DIPi | DIP-36020N |
IntActi | P0A847, 39 interactors |
STRINGi | 511145.b0406 |
Proteomic databases
jPOSTi | P0A847 |
PaxDbi | P0A847 |
PRIDEi | P0A847 |
Genome annotation databases
EnsemblBacteriai | AAC73509; AAC73509; b0406 BAE76186; BAE76186; BAE76186 |
GeneIDi | 58460351 949130 |
KEGGi | ecj:JW0396 eco:b0406 |
PATRICi | fig|1411691.4.peg.1871 |
Organism-specific databases
EchoBASEi | EB0989 |
Phylogenomic databases
eggNOGi | COG0343, Bacteria |
HOGENOMi | CLU_022060_0_1_6 |
InParanoidi | P0A847 |
PhylomeDBi | P0A847 |
Enzyme and pathway databases
UniPathwayi | UPA00392 |
BioCyci | EcoCyc:EG10996-MONOMER MetaCyc:EG10996-MONOMER |
BRENDAi | 2.4.2.29, 2026 |
SABIO-RKi | P0A847 |
Miscellaneous databases
PROi | PR:P0A847 |
Family and domain databases
Gene3Di | 3.20.20.105, 1 hit |
HAMAPi | MF_00168, Q_tRNA_Tgt, 1 hit |
InterProi | View protein in InterPro IPR004803, TGT IPR036511, TGT-like_sf IPR002616, tRNA_ribo_trans-like |
Pfami | View protein in Pfam PF01702, TGT, 1 hit |
SUPFAMi | SSF51713, SSF51713, 1 hit |
TIGRFAMsi | TIGR00430, Q_tRNA_tgt, 1 hit TIGR00449, tgt_general, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGT_ECOLI | |
Accessioni | P0A847Primary (citable) accession number: P0A847 Secondary accession number(s): P19675 Q47627 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families