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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.2 Publications

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium1
Metal bindingi67Magnesium1
Metal bindingi139Magnesium1
Metal bindingi142Magnesium1

GO - Molecular functioni

GO - Biological processi

  • recombinational repair Source: EcoCyc
  • replication fork reversal Source: GO_Central
  • response to radiation Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10925-MONOMER
MetaCyc:EG10925-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:b1863, JW1852
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10925 ruvC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001830962 – 173Crossover junction endodeoxyribonuclease RuvCAdd BLAST172

Proteomic databases

PaxDbiP0A814
PRIDEiP0A814

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4263235, 96 interactors
DIPiDIP-35952N
IntActiP0A814, 3 interactors
STRINGi316385.ECDH10B_2004

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A814
SMRiP0A814
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A814

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.Curated

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
InParanoidiP0A814
KOiK01159
PhylomeDBiP0A814

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A814-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA
60 70 80 90 100
GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF
110 120 130 140 150
EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH
160 170
CHVSQNAMQM SESRLNLARG RLR
Length:173
Mass (Da):18,747
Last modified:January 23, 2007 - v2
Checksum:i2170DCFA4AD43FE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59551 Genomic DNA Translation: CAA42128.1
D10165 Genomic DNA Translation: BAA01032.1
U00096 Genomic DNA Translation: AAC74933.1
AP009048 Genomic DNA Translation: BAA15674.1
PIRiD38113
RefSeqiNP_416377.1, NC_000913.3
WP_001295503.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74933; AAC74933; b1863
BAA15674; BAA15674; BAA15674
GeneIDi946378
KEGGiecj:JW1852
eco:b1863
PATRICifig|1411691.4.peg.385

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59551 Genomic DNA Translation: CAA42128.1
D10165 Genomic DNA Translation: BAA01032.1
U00096 Genomic DNA Translation: AAC74933.1
AP009048 Genomic DNA Translation: BAA15674.1
PIRiD38113
RefSeqiNP_416377.1, NC_000913.3
WP_001295503.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HJRX-ray2.50A/B/C/D2-159[»]
ProteinModelPortaliP0A814
SMRiP0A814
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263235, 96 interactors
DIPiDIP-35952N
IntActiP0A814, 3 interactors
STRINGi316385.ECDH10B_2004

Proteomic databases

PaxDbiP0A814
PRIDEiP0A814

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74933; AAC74933; b1863
BAA15674; BAA15674; BAA15674
GeneIDi946378
KEGGiecj:JW1852
eco:b1863
PATRICifig|1411691.4.peg.385

Organism-specific databases

EchoBASEiEB0918
EcoGeneiEG10925 ruvC

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
InParanoidiP0A814
KOiK01159
PhylomeDBiP0A814

Enzyme and pathway databases

BioCyciEcoCyc:EG10925-MONOMER
MetaCyc:EG10925-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A814
PROiPR:P0A814

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRUVC_ECOLI
AccessioniPrimary (citable) accession number: P0A814
Secondary accession number(s): P24239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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