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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Activity regulationi

RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • recombinational repair Source: EcoCyc
  • response to UV Source: EcoCyc
  • SOS response Source: EcoCyc

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10924-MONOMER
MetaCyc:EG10924-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:b1860, JW1849
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10924 ruvB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001655281 – 336Holliday junction ATP-dependent DNA helicase RuvBAdd BLAST336

Proteomic databases

PaxDbiP0A812
PRIDEiP0A812

Expressioni

Inductioni

Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals (PubMed:3279394). Induced by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Subunit structurei

Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium. Forms a complex with RuvA. Interacts with YgbT (Cas1).1 Publication

Protein-protein interaction databases

BioGridi4260882, 85 interactors
DIPiDIP-47870N
IntActiP0A812, 17 interactors
STRINGi316385.ECDH10B_2001

Structurei

3D structure databases

ProteinModelPortaliP0A812
SMRiP0A812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ Bacteria
COG2255 LUCA
HOGENOMiHOG000218623
InParanoidiP0A812
KOiK03551
PhylomeDBiP0A812

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00016 DNA_helic_RuvB, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR004605 DNA_helicase_Holl-junc_RuvB
IPR008823 DNA_helicase_Holl-junc_RuvB_C
IPR027417 P-loop_NTPase
IPR008824 RuvB_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR42848 PTHR42848, 1 hit
PfamiView protein in Pfam
PF05491 RuvB_C, 1 hit
PF05496 RuvB_N, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005323 DNA_helicase_Holl-junc_RuvB_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00635 ruvB, 1 hit

Sequencei

Sequence statusi: Complete.

P0A812-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL
60 70 80 90 100
RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM
110 120 130 140 150
LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI
160 170 180 190 200
DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM
210 220 230 240 250
GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA
260 270 280 290 300
LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV
310 320 330
LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP
Length:336
Mass (Da):37,174
Last modified:August 1, 1988 - v1
Checksum:i79BAB4A9A5687F7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA Translation: CAA30120.1
M21298 Genomic DNA Translation: AAA24613.1
U00096 Genomic DNA Translation: AAC74930.1
AP009048 Genomic DNA Translation: BAA15671.1
PIRiB28533 BVECVB
RefSeqiNP_416374.1, NC_000913.3
WP_000568519.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74930; AAC74930; b1860
BAA15671; BAA15671; BAA15671
GeneIDi946371
KEGGiecj:JW1849
eco:b1860
PATRICifig|1411691.4.peg.388

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA Translation: CAA30120.1
M21298 Genomic DNA Translation: AAA24613.1
U00096 Genomic DNA Translation: AAC74930.1
AP009048 Genomic DNA Translation: BAA15671.1
PIRiB28533 BVECVB
RefSeqiNP_416374.1, NC_000913.3
WP_000568519.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP0A812
SMRiP0A812
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260882, 85 interactors
DIPiDIP-47870N
IntActiP0A812, 17 interactors
STRINGi316385.ECDH10B_2001

Proteomic databases

PaxDbiP0A812
PRIDEiP0A812

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74930; AAC74930; b1860
BAA15671; BAA15671; BAA15671
GeneIDi946371
KEGGiecj:JW1849
eco:b1860
PATRICifig|1411691.4.peg.388

Organism-specific databases

EchoBASEiEB0917
EcoGeneiEG10924 ruvB

Phylogenomic databases

eggNOGiENOG4105CKJ Bacteria
COG2255 LUCA
HOGENOMiHOG000218623
InParanoidiP0A812
KOiK03551
PhylomeDBiP0A812

Enzyme and pathway databases

BioCyciEcoCyc:EG10924-MONOMER
MetaCyc:EG10924-MONOMER

Miscellaneous databases

PROiPR:P0A812

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00016 DNA_helic_RuvB, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR004605 DNA_helicase_Holl-junc_RuvB
IPR008823 DNA_helicase_Holl-junc_RuvB_C
IPR027417 P-loop_NTPase
IPR008824 RuvB_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR42848 PTHR42848, 1 hit
PfamiView protein in Pfam
PF05491 RuvB_C, 1 hit
PF05496 RuvB_N, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005323 DNA_helicase_Holl-junc_RuvB_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00635 ruvB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB_ECOLI
AccessioniPrimary (citable) accession number: P0A812
Secondary accession number(s): P08577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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