UniProtKB - P0A812 (RUVB_ECOLI)
Protein
Holliday junction ATP-dependent DNA helicase RuvB
Gene
ruvB
Organism
Escherichia coli (strain K12)
Status
Functioni
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation1 Publication
Catalytic activityi
- EC:3.6.4.12UniRule annotation1 Publication
Activity regulationi
RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 62 – 69 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: InterPro
- DNA helicase activity Source: CACAO
- four-way junction helicase activity Source: EcoCyc
GO - Biological processi
- recombinational repair Source: EcoCyc
- response to UV Source: EcoCyc
- SOS response Source: EcoCyc
Keywordsi
Molecular function | Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair, SOS response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10924-MONOMER MetaCyc:EG10924-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation) |
Gene namesi | Name:ruvBUniRule annotation Ordered Locus Names:b1860, JW1849 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.
Cytosol
- cytosol Source: EcoCyc
Other locations
- Holliday junction resolvase complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000165528 | 1 – 336 | Holliday junction ATP-dependent DNA helicase RuvBAdd BLAST | 336 |
Proteomic databases
jPOSTi | P0A812 |
PaxDbi | P0A812 |
PRIDEi | P0A812 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium.
Forms a complex with RuvA.
Interacts with YgbT (Cas1).
1 PublicationBinary interactionsi
P0A812
With | #Exp. | IntAct |
---|---|---|
ruvA [P0A809] | 5 | EBI-557878,EBI-555119 |
Protein-protein interaction databases
BioGRIDi | 4260882, 85 interactors |
ComplexPortali | CPX-5124, RuvAB Holliday junction DNA helicase complex |
DIPi | DIP-47870N |
IntActi | P0A812, 18 interactors |
STRINGi | 511145.b1860 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RuvB family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2255, Bacteria |
HOGENOMi | CLU_055599_1_0_6 |
InParanoidi | P0A812 |
PhylomeDBi | P0A812 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00016, DNA_helic_RuvB, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041445, AAA_lid_4 IPR004605, DNA_helicase_Holl-junc_RuvB IPR027417, P-loop_NTPase IPR008824, RuvB-like_N IPR008823, RuvB_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR42848, PTHR42848, 1 hit |
Pfami | View protein in Pfam PF17864, AAA_lid_4, 1 hit PF05491, RuvB_C, 1 hit PF05496, RuvB_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00635, ruvB, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A812-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL
60 70 80 90 100
RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM
110 120 130 140 150
LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI
160 170 180 190 200
DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM
210 220 230 240 250
GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA
260 270 280 290 300
LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV
310 320 330
LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07091 Genomic DNA Translation: CAA30120.1 M21298 Genomic DNA Translation: AAA24613.1 U00096 Genomic DNA Translation: AAC74930.1 AP009048 Genomic DNA Translation: BAA15671.1 |
PIRi | B28533, BVECVB |
RefSeqi | NP_416374.1, NC_000913.3 WP_000568519.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74930; AAC74930; b1860 BAA15671; BAA15671; BAA15671 |
GeneIDi | 58462246 946371 |
KEGGi | ecj:JW1849 eco:b1860 |
PATRICi | fig|1411691.4.peg.388 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07091 Genomic DNA Translation: CAA30120.1 M21298 Genomic DNA Translation: AAA24613.1 U00096 Genomic DNA Translation: AAC74930.1 AP009048 Genomic DNA Translation: BAA15671.1 |
PIRi | B28533, BVECVB |
RefSeqi | NP_416374.1, NC_000913.3 WP_000568519.1, NZ_STEB01000009.1 |
3D structure databases
SMRi | P0A812 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260882, 85 interactors |
ComplexPortali | CPX-5124, RuvAB Holliday junction DNA helicase complex |
DIPi | DIP-47870N |
IntActi | P0A812, 18 interactors |
STRINGi | 511145.b1860 |
Proteomic databases
jPOSTi | P0A812 |
PaxDbi | P0A812 |
PRIDEi | P0A812 |
Genome annotation databases
EnsemblBacteriai | AAC74930; AAC74930; b1860 BAA15671; BAA15671; BAA15671 |
GeneIDi | 58462246 946371 |
KEGGi | ecj:JW1849 eco:b1860 |
PATRICi | fig|1411691.4.peg.388 |
Organism-specific databases
EchoBASEi | EB0917 |
Phylogenomic databases
eggNOGi | COG2255, Bacteria |
HOGENOMi | CLU_055599_1_0_6 |
InParanoidi | P0A812 |
PhylomeDBi | P0A812 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10924-MONOMER MetaCyc:EG10924-MONOMER |
Miscellaneous databases
PROi | PR:P0A812 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00016, DNA_helic_RuvB, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041445, AAA_lid_4 IPR004605, DNA_helicase_Holl-junc_RuvB IPR027417, P-loop_NTPase IPR008824, RuvB-like_N IPR008823, RuvB_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR42848, PTHR42848, 1 hit |
Pfami | View protein in Pfam PF17864, AAA_lid_4, 1 hit PF05491, RuvB_C, 1 hit PF05496, RuvB_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00635, ruvB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUVB_ECOLI | |
Accessioni | P0A812Primary (citable) accession number: P0A812 Secondary accession number(s): P08577 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families