UniProtKB - P0A809 (RUVA_ECOLI)
Protein
Holliday junction ATP-dependent DNA helicase RuvA
Gene
ruvA
Organism
Escherichia coli (strain K12)
Status
Functioni
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.UniRule annotation1 Publication
Catalytic activityi
- EC:3.6.4.12UniRule annotation1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- four-way junction DNA binding Source: EcoCyc
- four-way junction helicase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
GO - Biological processi
- recombinational repair Source: EcoCyc
- response to radiation Source: EcoCyc
- SOS response Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair, SOS response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10923-MONOMER MetaCyc:EG10923-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation) |
Gene namesi | Name:ruvAUniRule annotation Ordered Locus Names:b1861, JW1850 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.
Other locations
- cytoplasm Source: UniProtKB-SubCell
- Holliday junction helicase complex Source: EcoCyc
- Holliday junction resolvase complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094629 | 1 – 203 | Holliday junction ATP-dependent DNA helicase RuvAAdd BLAST | 203 |
Proteomic databases
jPOSTi | P0A809 |
PaxDbi | P0A809 |
PRIDEi | P0A809 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homotetramer; forms a complex with RuvB.
2 PublicationsBinary interactionsi
Hide detailsP0A809
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-555119,EBI-555119 |
ruvB [P0A812] | 5 | EBI-555119,EBI-557878 |
ruvC [P0A814] | 4 | EBI-555119,EBI-1123014 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259338, 146 interactors 850726, 1 interactor |
ComplexPortali | CPX-5124, RuvAB Holliday junction DNA helicase complex |
DIPi | DIP-48064N |
IntActi | P0A809, 9 interactors |
STRINGi | 511145.b1861 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A809 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A809 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RuvA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0632, Bacteria |
HOGENOMi | CLU_087936_0_0_6 |
InParanoidi | P0A809 |
PhylomeDBi | P0A809 |
Family and domain databases
CDDi | cd14332, UBA_RuvA_C, 1 hit |
HAMAPi | MF_00031, DNA_helic_RuvA, 1 hit |
InterProi | View protein in InterPro IPR013849, DNA_helicase_Holl-junc_RuvA_I IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR012340, NA-bd_OB-fold IPR000085, RuvA IPR010994, RuvA_2-like IPR011114, RuvA_C IPR036267, RuvA_C_sf |
Pfami | View protein in Pfam PF07499, RuvA_C, 1 hit PF01330, RuvA_N, 1 hit |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits |
SUPFAMi | SSF46929, SSF46929, 1 hit SSF47781, SSF47781, 1 hit SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00084, ruvA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A809-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH
60 70 80 90 100
FVVREDAQLL YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN
110 120 130 140 150
AVEREEVGAL VKLPGIGKKT AERLIVEMKD RFKGLHGDLF TPAADLVLTS
160 170 180 190 200
PASPATDDAE QEAVAALVAL GYKPQEASRM VSKIARPDAS SETLIREALR
AAL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 | A → R in CAA30119 (PubMed:3279394).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07091 Genomic DNA Translation: CAA30119.1 M21298 Genomic DNA Translation: AAA24612.1 U00096 Genomic DNA Translation: AAC74931.1 AP009048 Genomic DNA Translation: BAA15672.1 D10165 Genomic DNA Translation: BAA01034.1 |
PIRi | E64948, BVECRV |
RefSeqi | NP_416375.1, NC_000913.3 WP_000580323.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74931; AAC74931; b1861 BAA15672; BAA15672; BAA15672 |
GeneIDi | 58346771 946369 |
KEGGi | ecj:JW1850 eco:b1861 |
PATRICi | fig|1411691.4.peg.387 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07091 Genomic DNA Translation: CAA30119.1 M21298 Genomic DNA Translation: AAA24612.1 U00096 Genomic DNA Translation: AAC74931.1 AP009048 Genomic DNA Translation: BAA15672.1 D10165 Genomic DNA Translation: BAA01034.1 |
PIRi | E64948, BVECRV |
RefSeqi | NP_416375.1, NC_000913.3 WP_000580323.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BDX | X-ray | 6.00 | A/B/C/D | 1-203 | [»] | |
1C7Y | X-ray | 3.10 | A | 1-203 | [»] | |
1CUK | X-ray | 1.90 | A | 1-203 | [»] | |
1D8L | X-ray | 2.50 | A/B | 1-149 | [»] | |
1HJP | X-ray | 2.50 | A | 1-203 | [»] | |
SMRi | P0A809 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259338, 146 interactors 850726, 1 interactor |
ComplexPortali | CPX-5124, RuvAB Holliday junction DNA helicase complex |
DIPi | DIP-48064N |
IntActi | P0A809, 9 interactors |
STRINGi | 511145.b1861 |
Proteomic databases
jPOSTi | P0A809 |
PaxDbi | P0A809 |
PRIDEi | P0A809 |
Genome annotation databases
EnsemblBacteriai | AAC74931; AAC74931; b1861 BAA15672; BAA15672; BAA15672 |
GeneIDi | 58346771 946369 |
KEGGi | ecj:JW1850 eco:b1861 |
PATRICi | fig|1411691.4.peg.387 |
Organism-specific databases
EchoBASEi | EB0916 |
Phylogenomic databases
eggNOGi | COG0632, Bacteria |
HOGENOMi | CLU_087936_0_0_6 |
InParanoidi | P0A809 |
PhylomeDBi | P0A809 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10923-MONOMER MetaCyc:EG10923-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A809 |
PROi | PR:P0A809 |
Family and domain databases
CDDi | cd14332, UBA_RuvA_C, 1 hit |
HAMAPi | MF_00031, DNA_helic_RuvA, 1 hit |
InterProi | View protein in InterPro IPR013849, DNA_helicase_Holl-junc_RuvA_I IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR012340, NA-bd_OB-fold IPR000085, RuvA IPR010994, RuvA_2-like IPR011114, RuvA_C IPR036267, RuvA_C_sf |
Pfami | View protein in Pfam PF07499, RuvA_C, 1 hit PF01330, RuvA_N, 1 hit |
SMARTi | View protein in SMART SM00278, HhH1, 2 hits |
SUPFAMi | SSF46929, SSF46929, 1 hit SSF47781, SSF47781, 1 hit SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00084, ruvA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUVA_ECOLI | |
Accessioni | P0A809Primary (citable) accession number: P0A809 Secondary accession number(s): P08576 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families