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UniProtKB - P0A7Y8 (RNPA_ECOLI)
Protein
Ribonuclease P protein component
Gene
rnpA
Organism
Escherichia coli (strain K12)
Status
Functioni
RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.
UniRule annotation1 PublicationCatalytic activityi
- Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation1 Publication EC:3.1.26.5
GO - Molecular functioni
- 3'-tRNA processing endoribonuclease activity Source: GO_Central
- ribonuclease P activity Source: GO_Central
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- RNA processing Source: EcoliWiki
- tRNA 3'-trailer cleavage, endonucleolytic Source: GO_Central
- tRNA 5'-leader removal Source: EcoliWiki
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease, RNA-binding |
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10862-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease P protein componentUniRule annotation (EC:3.1.26.5UniRule annotation)Short name: RNase P proteinUniRule annotation Short name: RNaseP proteinUniRule annotation Alternative name(s): Protein C5UniRule annotation |
Gene namesi | Name:rnpAUniRule annotation Ordered Locus Names:b3704, JW3681 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198458 | 1 – 119 | Ribonuclease P protein componentAdd BLAST | 119 |
Proteomic databases
jPOSTi | P0A7Y8 |
PaxDbi | P0A7Y8 |
PRIDEi | P0A7Y8 |
Interactioni
Subunit structurei
Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4261766, 36 interactors 852517, 2 interactors |
DIPi | DIP-36039N |
IntActi | P0A7Y8, 60 interactors |
STRINGi | 511145.b3704 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7Y8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the RnpA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0594, Bacteria |
HOGENOMi | CLU_117179_11_0_6 |
InParanoidi | P0A7Y8 |
PhylomeDBi | P0A7Y8 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_00227, RNase_P, 1 hit |
InterProi | View protein in InterPro IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR000100, RNase_P IPR020539, RNase_P_CS |
PANTHERi | PTHR33992, PTHR33992, 1 hit |
Pfami | View protein in Pfam PF00825, Ribonuclease_P, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit |
TIGRFAMsi | TIGR00188, rnpA, 1 hit |
PROSITEi | View protein in PROSITE PS00648, RIBONUCLEASE_P, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7Y8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT
60 70 80 90 100
VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA
110
LSEALEKLWR RHCRLARGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11056 Genomic DNA Translation: AAA24567.1 X01861 Genomic DNA Translation: CAA25983.1 L10328 Genomic DNA Translation: AAA62055.1 U00096 Genomic DNA Translation: AAC76727.1 AP009048 Genomic DNA Translation: BAE77590.1 |
PIRi | A00794, NRECP |
RefSeqi | NP_418159.1, NC_000913.3 WP_000239730.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76727; AAC76727; b3704 BAE77590; BAE77590; BAE77590 |
GeneIDi | 67417694 948215 |
KEGGi | ecj:JW3681 eco:b3704 |
PATRICi | fig|511145.12.peg.3828 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11056 Genomic DNA Translation: AAA24567.1 X01861 Genomic DNA Translation: CAA25983.1 L10328 Genomic DNA Translation: AAA62055.1 U00096 Genomic DNA Translation: AAC76727.1 AP009048 Genomic DNA Translation: BAE77590.1 |
PIRi | A00794, NRECP |
RefSeqi | NP_418159.1, NC_000913.3 WP_000239730.1, NZ_STEB01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LJP | NMR | - | A | 1-119 | [»] | |
SMRi | P0A7Y8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261766, 36 interactors 852517, 2 interactors |
DIPi | DIP-36039N |
IntActi | P0A7Y8, 60 interactors |
STRINGi | 511145.b3704 |
Proteomic databases
jPOSTi | P0A7Y8 |
PaxDbi | P0A7Y8 |
PRIDEi | P0A7Y8 |
Genome annotation databases
EnsemblBacteriai | AAC76727; AAC76727; b3704 BAE77590; BAE77590; BAE77590 |
GeneIDi | 67417694 948215 |
KEGGi | ecj:JW3681 eco:b3704 |
PATRICi | fig|511145.12.peg.3828 |
Organism-specific databases
EchoBASEi | EB0855 |
Phylogenomic databases
eggNOGi | COG0594, Bacteria |
HOGENOMi | CLU_117179_11_0_6 |
InParanoidi | P0A7Y8 |
PhylomeDBi | P0A7Y8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10862-MONOMER |
Miscellaneous databases
PROi | PR:P0A7Y8 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_00227, RNase_P, 1 hit |
InterProi | View protein in InterPro IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR000100, RNase_P IPR020539, RNase_P_CS |
PANTHERi | PTHR33992, PTHR33992, 1 hit |
Pfami | View protein in Pfam PF00825, Ribonuclease_P, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit |
TIGRFAMsi | TIGR00188, rnpA, 1 hit |
PROSITEi | View protein in PROSITE PS00648, RIBONUCLEASE_P, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RNPA_ECOLI | |
Accessioni | P0A7Y8Primary (citable) accession number: P0A7Y8 Secondary accession number(s): P06277, Q2M816 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families