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Protein

30S ribosomal protein S8

Gene

rpsH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.By similarity
Protein S8 is a translational repressor protein, it controls the translation of the spc operon by binding to its mRNA.1 Publication

GO - Molecular functioni

  • rRNA binding Source: EcoliWiki
  • structural constituent of ribosome Source: CAFA

GO - Biological processi

  • regulation of mRNA stability Source: EcoliWiki
  • regulation of translation Source: UniProtKB-KW
  • ribosomal small subunit assembly Source: CAFA
  • translation Source: UniProtKB-UniRule

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10907-MONOMER
MetaCyc:EG10907-MONOMER

Protein family/group databases

MoonProtiP0A7W7

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S8
Alternative name(s):
Small ribosomal subunit protein uS81 Publication
Gene namesi
Name:rpsH
Ordered Locus Names:b3306, JW3268
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10907 rpsH

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB00759 Tetracycline

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001264052 – 13030S ribosomal protein S8Add BLAST129

Proteomic databases

EPDiP0A7W7
PaxDbiP0A7W7
PRIDEiP0A7W7

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit (PubMed:365698, PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:12244297, PubMed:27906160, PubMed:27906161, PubMed:28077875). Contacts proteins S5 and S12 (By similarity).By similarity9 Publications

Protein-protein interaction databases

BioGridi852114, 1 interactor
DIPiDIP-47901N
IntActiP0A7W7, 43 interactors
STRINGi316385.ECDH10B_3481

Structurei

Secondary structure

1130
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A7W7
SMRiP0A7W7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7W7

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0096 LUCA
HOGENOMiHOG000204095
InParanoidiP0A7W7
KOiK02994
PhylomeDBiP0A7W7

Family and domain databases

HAMAPiMF_01302_B Ribosomal_S8_B, 1 hit
InterProiView protein in InterPro
IPR000630 Ribosomal_S8
IPR035987 Ribosomal_S8_sf
PANTHERiPTHR11758 PTHR11758, 1 hit
PfamiView protein in Pfam
PF00410 Ribosomal_S8, 1 hit
SUPFAMiSSF56047 SSF56047, 1 hit
PROSITEiView protein in PROSITE
PS00053 RIBOSOMAL_S8, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7W7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK
60 70 80 90 100
VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRKD ELPKVMAGLG
110 120 130
IAVVSTSKGV MTDRAARQAG LGGEIICYVA
Length:130
Mass (Da):14,127
Last modified:January 23, 2007 - v2
Checksum:i74641BC5021DA651
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91E → Q in CAA25719 (PubMed:6222285).Curated1

Mass spectrometryi

Molecular mass is 13993.2 Da from positions 2 - 130. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01563 Genomic DNA Translation: CAA25719.1
U18997 Genomic DNA Translation: AAA58103.1
U00096 Genomic DNA Translation: AAC76331.1
AP009048 Genomic DNA Translation: BAE77985.1
PIRiE65123 R3EC8
RefSeqiNP_417765.1, NC_000913.3
WP_000062611.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76331; AAC76331; b3306
BAE77985; BAE77985; BAE77985
GeneIDi947802
KEGGiecj:JW3268
eco:b3306
PATRICifig|1411691.4.peg.3425

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01563 Genomic DNA Translation: CAA25719.1
U18997 Genomic DNA Translation: AAA58103.1
U00096 Genomic DNA Translation: AAC76331.1
AP009048 Genomic DNA Translation: BAE77985.1
PIRiE65123 R3EC8
RefSeqiNP_417765.1, NC_000913.3
WP_000062611.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EG0electron microscopy11.50E1-130[»]
1M5Gmodel-H2-130[»]
1S03X-ray2.70G/H2-130[»]
2YKRelectron microscopy9.80H2-130[»]
3J9Yelectron microscopy3.90h1-130[»]
3J9Zelectron microscopy3.60SH2-130[»]
3JA1electron microscopy3.60SH2-130[»]
3JBUelectron microscopy3.64H1-130[»]
3JBVelectron microscopy3.32H1-130[»]
3JCDelectron microscopy3.70h1-130[»]
3JCEelectron microscopy3.20h1-130[»]
3JCJelectron microscopy3.70p1-130[»]
3JCNelectron microscopy4.60k1-130[»]
4A2Ielectron microscopy16.50H2-130[»]
4ADVelectron microscopy13.50H2-130[»]
4U1UX-ray2.95AH/CH2-130[»]
4U1VX-ray3.00AH/CH2-130[»]
4U20X-ray2.90AH/CH2-130[»]
4U24X-ray2.90AH/CH2-130[»]
4U25X-ray2.90AH/CH2-130[»]
4U26X-ray2.80AH/CH2-130[»]
4U27X-ray2.80AH/CH2-130[»]
4V47electron microscopy12.30BH2-130[»]
4V48electron microscopy11.50BH2-130[»]
4V4HX-ray3.46AH/CH1-130[»]
4V4QX-ray3.46AH/CH2-130[»]
4V4Velectron microscopy15.00AH3-129[»]
4V4Welectron microscopy15.00AH3-129[»]
4V50X-ray3.22AH/CH2-130[»]
4V52X-ray3.21AH/CH2-130[»]
4V53X-ray3.54AH/CH2-130[»]
4V54X-ray3.30AH/CH2-130[»]
4V55X-ray4.00AH/CH2-130[»]
4V56X-ray3.93AH/CH2-130[»]
4V57X-ray3.50AH/CH2-130[»]
4V5BX-ray3.74BH/DH2-130[»]
4V5Helectron microscopy5.80AH2-130[»]
4V5YX-ray4.45AH/CH2-130[»]
4V64X-ray3.50AH/CH2-130[»]
4V65electron microscopy9.00AV1-130[»]
4V66electron microscopy9.00AV1-130[»]
4V69electron microscopy6.70AH2-130[»]
4V6CX-ray3.19AH/CH1-130[»]
4V6DX-ray3.81AH/CH1-130[»]
4V6EX-ray3.71AH/CH1-130[»]
4V6Kelectron microscopy8.25BL1-130[»]
4V6Lelectron microscopy13.20AL1-130[»]
4V6Melectron microscopy7.10AH2-130[»]
4V6Nelectron microscopy12.10BK2-130[»]
4V6Oelectron microscopy14.70AK2-130[»]
4V6Pelectron microscopy13.50AK2-130[»]
4V6Qelectron microscopy11.50AK2-130[»]
4V6Relectron microscopy11.50AK2-130[»]
4V6Selectron microscopy13.10BJ2-130[»]
4V6Telectron microscopy8.30AH2-130[»]
4V6Velectron microscopy9.80AH2-130[»]
4V6Yelectron microscopy12.00AH1-130[»]
4V6Zelectron microscopy12.00AH1-130[»]
4V70electron microscopy17.00AH1-130[»]
4V71electron microscopy20.00AH1-130[»]
4V72electron microscopy13.00AH1-130[»]
4V73electron microscopy15.00AH1-130[»]
4V74electron microscopy17.00AH1-130[»]
4V75electron microscopy12.00AH1-130[»]
4V76electron microscopy17.00AH1-130[»]
4V77electron microscopy17.00AH1-130[»]
4V78electron microscopy20.00AH1-130[»]
4V79electron microscopy15.00AH1-130[»]
4V7Aelectron microscopy9.00AH1-130[»]
4V7Belectron microscopy6.80AH1-130[»]
4V7Celectron microscopy7.60AH2-130[»]
4V7Delectron microscopy7.60BH2-130[»]
4V7Ielectron microscopy9.60BH1-130[»]
4V7SX-ray3.25AH/CH2-130[»]
4V7TX-ray3.19AH/CH2-130[»]
4V7UX-ray3.10AH/CH2-130[»]
4V7VX-ray3.29AH/CH2-130[»]
4V85X-ray3.20H1-130[»]
4V89X-ray3.70AH1-130[»]
4V9CX-ray3.30AH/CH1-130[»]
4V9DX-ray3.00AH/BH2-130[»]
4V9OX-ray2.90BH/DH/FH/HH1-130[»]
4V9PX-ray2.90BH/DH/FH/HH1-130[»]
4WF1X-ray3.09AH/CH2-130[»]
4WOIX-ray3.00AH/DH1-130[»]
4WWWX-ray3.10QH/XH2-130[»]
4YBBX-ray2.10AH/BH2-130[»]
5AFIelectron microscopy2.90h1-130[»]
5H5Uelectron microscopy3.00o2-130[»]
5IQRelectron microscopy3.00m1-130[»]
5IT8X-ray3.12AH/BH2-130[»]
5J5BX-ray2.80AH/BH2-130[»]
5J7LX-ray3.00AH/BH2-130[»]
5J88X-ray3.32AH/BH2-130[»]
5J8AX-ray3.10AH/BH2-130[»]
5J91X-ray2.96AH/BH2-130[»]
5JC9X-ray3.03AH/BH2-130[»]
5JTEelectron microscopy3.60AH1-130[»]
5JU8electron microscopy3.60AH1-130[»]
5KCRelectron microscopy3.601h1-130[»]
5KCSelectron microscopy3.901h1-130[»]
5KPSelectron microscopy3.90131-130[»]
5KPVelectron microscopy4.10121-130[»]
5KPWelectron microscopy3.90121-130[»]
5KPXelectron microscopy3.90121-130[»]
5L3Pelectron microscopy3.70h1-130[»]
5LZAelectron microscopy3.60h2-130[»]
5LZBelectron microscopy5.30h2-130[»]
5LZCelectron microscopy4.80h2-130[»]
5LZDelectron microscopy3.40h2-130[»]
5LZEelectron microscopy3.50h2-130[»]
5LZFelectron microscopy4.60h2-130[»]
5MDVelectron microscopy2.97m1-130[»]
5MDWelectron microscopy3.06m1-130[»]
5MDYelectron microscopy3.35m1-130[»]
5MDZelectron microscopy3.10m1-130[»]
5ME0electron microscopy13.50H1-130[»]
5ME1electron microscopy13.50H1-130[»]
5MGPelectron microscopy3.10h2-130[»]
5MY1electron microscopy7.60H2-130[»]
5NO2electron microscopy5.16H2-130[»]
5NO3electron microscopy5.16H2-130[»]
5NO4electron microscopy5.16H2-130[»]
5NP6electron microscopy3.60K2-130[»]
5NWYelectron microscopy2.9371-130[»]
5O2Relectron microscopy3.40h2-130[»]
5U4Ielectron microscopy3.50h2-130[»]
5U9Felectron microscopy3.20H1-130[»]
5U9Gelectron microscopy3.20H1-130[»]
5UYKelectron microscopy3.90H2-130[»]
5UYLelectron microscopy3.60H2-130[»]
5UYMelectron microscopy3.20H2-130[»]
5UYNelectron microscopy4.00H2-130[»]
5UYPelectron microscopy3.90H2-130[»]
5UYQelectron microscopy3.80H2-130[»]
5WDTelectron microscopy3.00h2-130[»]
5WE4electron microscopy3.10h2-130[»]
5WE6electron microscopy3.40h2-130[»]
5WFKelectron microscopy3.40h2-130[»]
6BU8electron microscopy3.50H2-130[»]
6C4Ielectron microscopy3.24h1-130[»]
6ENFelectron microscopy3.20h2-130[»]
6ENJelectron microscopy3.70h2-130[»]
6ENUelectron microscopy3.10h2-130[»]
6GWTelectron microscopy3.80h2-130[»]
6GXMelectron microscopy3.80h2-130[»]
6GXNelectron microscopy3.90h2-130[»]
6GXOelectron microscopy3.90h2-130[»]
6GXPelectron microscopy4.40h2-130[»]
6H4Nelectron microscopy3.00h2-130[»]
6H58electron microscopy7.90h/hh2-130[»]
ProteinModelPortaliP0A7W7
SMRiP0A7W7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi852114, 1 interactor
DIPiDIP-47901N
IntActiP0A7W7, 43 interactors
STRINGi316385.ECDH10B_3481

Chemistry databases

DrugBankiDB00759 Tetracycline

Protein family/group databases

MoonProtiP0A7W7

Proteomic databases

EPDiP0A7W7
PaxDbiP0A7W7
PRIDEiP0A7W7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76331; AAC76331; b3306
BAE77985; BAE77985; BAE77985
GeneIDi947802
KEGGiecj:JW3268
eco:b3306
PATRICifig|1411691.4.peg.3425

Organism-specific databases

EchoBASEiEB0900
EcoGeneiEG10907 rpsH

Phylogenomic databases

eggNOGiCOG0096 LUCA
HOGENOMiHOG000204095
InParanoidiP0A7W7
KOiK02994
PhylomeDBiP0A7W7

Enzyme and pathway databases

BioCyciEcoCyc:EG10907-MONOMER
MetaCyc:EG10907-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A7W7
PROiPR:P0A7W7

Family and domain databases

HAMAPiMF_01302_B Ribosomal_S8_B, 1 hit
InterProiView protein in InterPro
IPR000630 Ribosomal_S8
IPR035987 Ribosomal_S8_sf
PANTHERiPTHR11758 PTHR11758, 1 hit
PfamiView protein in Pfam
PF00410 Ribosomal_S8, 1 hit
SUPFAMiSSF56047 SSF56047, 1 hit
PROSITEiView protein in PROSITE
PS00053 RIBOSOMAL_S8, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRS8_ECOLI
AccessioniPrimary (citable) accession number: P0A7W7
Secondary accession number(s): P02361, Q2M6X1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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