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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity1 Publication
Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.1 Publication

Miscellaneous

At least 19 substitutions or deletions in 11 codons can promote streptomycin resistance, dependence or pseudodependence; all but one of the streptomycin resistant mutations (K42R) are associated with hyperaccurate translation and thus reduced translational efficiency.2 Publications
The streptomycin sensitive allele is dominant to resistant alleles.

GO - Molecular functioni

  • misfolded RNA binding Source: EcoCyc
  • rRNA binding Source: EcoCyc
  • structural constituent of ribosome Source: CAFA
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • Group I intron splicing Source: EcoCyc
  • maintenance of translational fidelity Source: EcoCyc
  • positive regulation of RNA splicing Source: EcoCyc
  • response to antibiotic Source: UniProtKB-KW
  • RNA folding Source: EcoCyc
  • translation Source: EcoCyc

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding
Biological processAntibiotic resistance

Enzyme and pathway databases

BioCyciEcoCyc:EG10911-MONOMER
MetaCyc:EG10911-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Alternative name(s):
Small ribosomal subunit protein uS121 Publication
Gene namesi
Name:rpsL
Synonyms:strA
Ordered Locus Names:b3342, JW3304
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10911 rpsL

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • cytosolic small ribosomal subunit Source: EcoliWiki

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57L → H: Protein is not incorporated into ribosomes. 1 Publication1
Mutagenesisi88K → Q: Confers low-level resistance to streptomycin and a 15% decrease in the translational elongation rate. 1 Publication1

Chemistry databases

DrugBankiDB00479 Amikacin
DB06696 Arbekacin
DB00452 Framycetin
DB00798 Gentamicin
DB04729 GENTAMICIN C1A
DB01172 Kanamycin
DB00994 Neomycin
DB00955 Netilmicin
DB03615 Ribostamycin
DB00919 Spectinomycin
DB01082 Streptomycin
DB00560 Tigecycline
DB00684 Tobramycin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001462192 – 12430S ribosomal protein S12Add BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei893-methylthioaspartic acid1 Publication1
Modified residuei108N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

EPDiP0A7S3
PaxDbiP0A7S3
PRIDEiP0A7S3

PTM databases

iPTMnetiP0A7S3

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit (PubMed:320034, PubMed:7556101, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:10094780, PubMed:12093756, PubMed:12379845, PubMed:12244297, PubMed:27906160, PubMed:27906161). Contacts proteins S8 and S17. Interacts with ArfA (PubMed:27906160, PubMed:27906161). May interact with IF1 in the 30S initiation complex (By similarity).By similarity11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rimPP0A8A83EBI-543960,EBI-561065

Protein-protein interaction databases

BioGridi852156, 1 interactor
DIPiDIP-35806N
IntActiP0A7S3, 29 interactors
STRINGi316385.ECDH10B_p2

Structurei

Secondary structure

1124
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 9Combined sources6
Turni24 – 26Combined sources3
Beta strandi30 – 36Combined sources7
Beta strandi37 – 40Combined sources4
Beta strandi43 – 45Combined sources3
Beta strandi52 – 57Combined sources6
Turni58 – 60Combined sources3
Beta strandi62 – 66Combined sources5
Beta strandi69 – 71Combined sources3
Beta strandi79 – 84Combined sources6
Beta strandi90 – 92Combined sources3
Beta strandi95 – 97Combined sources3
Beta strandi99 – 101Combined sources3
Turni114 – 118Combined sources5

3D structure databases

DisProtiDP00145
ProteinModelPortaliP0A7S3
SMRiP0A7S3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7S3

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0048 LUCA
HOGENOMiHOG000040063
InParanoidiP0A7S3
KOiK02950
OMAiKRGVCTA
PhylomeDBiP0A7S3

Family and domain databases

CDDicd03368 Ribosomal_S12, 1 hit
HAMAPiMF_00403_B Ribosomal_S12_B, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR006032 Ribosomal_S12/S23
IPR005679 Ribosomal_S12_bac
PANTHERiPTHR11652 PTHR11652, 1 hit
PfamiView protein in Pfam
PF00164 Ribosom_S12_S23, 1 hit
PIRSFiPIRSF002133 Ribosomal_S12/S23, 1 hit
PRINTSiPR01034 RIBOSOMALS12
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00981 rpsL_bact, 1 hit
PROSITEiView protein in PROSITE
PS00055 RIBOSOMAL_S12, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR
60 70 80 90 100
KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVKDL PGVRYHTVRG
110 120
ALDCSGVKDR KQARSKYGVK RPKA
Length:124
Mass (Da):13,737
Last modified:January 23, 2007 - v2
Checksum:i94A57F4C4FF0FC5E
GO

Mass spectrometryi

Molecular mass is 13651.3 Da from positions 2 - 124. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti43K → R Confers streptomycin resistance but not hyperaccurate translation. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00355 Genomic DNA Translation: CAA23648.1
U18997 Genomic DNA Translation: AAA58139.1
U00096 Genomic DNA Translation: AAC76367.1
AP009048 Genomic DNA Translation: BAE77949.1
AF312716 Genomic DNA Translation: AAG30936.1
AF312717 Genomic DNA Translation: AAG30937.1
V00354 Genomic DNA Translation: CAA23647.1
J01688 Genomic DNA Translation: AAA50988.1
PIRiS13738 R3EC12
RefSeqiNP_417801.1, NC_000913.3
WP_000246815.1, NZ_CP011343.2

Genome annotation databases

EnsemblBacteriaiAAC76367; AAC76367; b3342
BAE77949; BAE77949; BAE77949
GeneIDi34154647
947845
KEGGiecj:JW3304
eco:b3342
PATRICifig|1411691.4.peg.3389

Similar proteinsi

Entry informationi

Entry nameiRS12_ECOLI
AccessioniPrimary (citable) accession number: P0A7S3
Secondary accession number(s): P02367, Q2M707, Q9F5N3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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