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UniProtKB - P0A7M9 (RL31_ECOLI)
Protein
50S ribosomal protein L31
Gene
rpmE
Organism
Escherichia coli (strain K12)
Status
Functioni
Binds the 23S rRNA.
By similarityCofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit. Only 1 ligand appears to be Cys, the other are thought to be His and either backbone amides or solvent (PubMed:22196016).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 16 | ZincCurated | 1 |
GO - Molecular functioni
- rRNA binding Source: UniProtKB-KW
- structural constituent of ribosome Source: InterPro
- zinc ion binding Source: EcoCyc
GO - Biological processi
- cytoplasmic translation Source: ComplexPortal
- negative regulation of cytoplasmic translational initiation Source: EcoCyc
- translation Source: EcoCyc
- translational initiation Source: EcoCyc
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10889-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 50S ribosomal protein L31Alternative name(s): Large ribosomal subunit protein bL31-A1 Publication |
Gene namesi | Name:rpmE Ordered Locus Names:b3936, JW3907 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
- cytosolic large ribosomal subunit Source: EcoCyc
Other locations
- cytoplasm Source: ComplexPortal
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 | C → S: No Zn(2+) binding. 1 Publication | 1 | |
Mutagenesisi | 18 | C → S: Binds Zn(2+) normally. 1 Publication | 1 | |
Mutagenesisi | 37 – 40 | CSKC → SSKS: Binds Zn(2+) normally. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173102 | 1 – 70 | 50S ribosomal protein L31Add BLAST | 70 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | N6-acetyllysine1 Publication | 1 |
Post-translational modificationi
Proteolytically cleaved by protease VII to yield a peptide lacking residues 63-70. It is not clear if this is due to protein degradation or is a bona fide processing event in the strain used in PubMed:339950 and PubMed:10556732. In strains B, D10, MRE-600 and Q13 the only protein seen in PubMed:10556732 was full-length; the last 7 amino acids were sequenced only for strain MRE-600.2 Publications
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0A7M9 |
PaxDbi | P0A7M9 |
PRIDEi | P0A7M9 |
PTM databases
iPTMneti | P0A7M9 |
Interactioni
Subunit structurei
Part of the 50S ribosomal subunit.
6 PublicationsProtein-protein interaction databases
BioGRIDi | 4261184, 44 interactors 852722, 7 interactors |
ComplexPortali | CPX-3807, 50S large ribosomal subunit |
IntActi | P0A7M9, 25 interactors |
STRINGi | 511145.b3936 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A7M9 |
SMRi | P0A7M9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7M9 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0254, Bacteria |
HOGENOMi | CLU_114306_4_3_6 |
InParanoidi | P0A7M9 |
OMAi | WYPDAKV |
PhylomeDBi | P0A7M9 |
Family and domain databases
Gene3Di | 4.10.830.30, 1 hit |
HAMAPi | MF_00501, Ribosomal_L31_1, 1 hit |
InterProi | View protein in InterPro IPR034704, L28p-like IPR002150, Ribosomal_L31 IPR027491, Ribosomal_L31_A IPR042105, Ribosomal_L31_sf |
PANTHERi | PTHR33280, PTHR33280, 1 hit |
Pfami | View protein in Pfam PF01197, Ribosomal_L31, 1 hit |
PRINTSi | PR01249, RIBOSOMALL31 |
SUPFAMi | SSF143800, SSF143800, 1 hit |
TIGRFAMsi | TIGR00105, L31, 1 hit |
PROSITEi | View protein in PROSITE PS01143, RIBOSOMAL_L31, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7M9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD
60 70
VATGGRVDRF NKRFNIPGSK
Mass spectrometryi
Molecular mass is 7871.0 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78541 Genomic DNA Translation: CAA55286.1 L19201 Genomic DNA Translation: AAB03068.1 U00096 Genomic DNA Translation: AAC76918.1 AP009048 Genomic DNA Translation: BAE77374.1 |
PIRi | S40879, R5EC31 |
RefSeqi | NP_418371.1, NC_000913.3 WP_000710769.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76918; AAC76918; b3936 BAE77374; BAE77374; BAE77374 |
GeneIDi | 66672156 948425 |
KEGGi | ecj:JW3907 eco:b3936 |
PATRICi | fig|1411691.4.peg.2769 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78541 Genomic DNA Translation: CAA55286.1 L19201 Genomic DNA Translation: AAB03068.1 U00096 Genomic DNA Translation: AAC76918.1 AP009048 Genomic DNA Translation: BAE77374.1 |
PIRi | S40879, R5EC31 |
RefSeqi | NP_418371.1, NC_000913.3 WP_000710769.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2J28 | electron microscopy | 8.00 | Z | 1-70 | [»] | |
2RDO | electron microscopy | 9.10 | Z | 1-70 | [»] | |
3BBX | electron microscopy | 10.00 | Z | 1-70 | [»] | |
3J9Y | electron microscopy | 3.90 | 6 | 1-70 | [»] | |
3J9Z | electron microscopy | 3.60 | LZ | 1-70 | [»] | |
3JA1 | electron microscopy | 3.60 | L2 | 1-70 | [»] | |
4V4H | X-ray | 3.46 | BZ/DZ | 1-70 | [»] | |
4V4Q | X-ray | 3.46 | BZ/DZ | 1-70 | [»] | |
4V5B | X-ray | 3.74 | AZ/CZ | 1-70 | [»] | |
4V65 | electron microscopy | 9.00 | BS | 1-70 | [»] | |
4V66 | electron microscopy | 9.00 | BS | 1-70 | [»] | |
4V6K | electron microscopy | 8.25 | Ab | 1-70 | [»] | |
4V6L | electron microscopy | 13.20 | Bb | 1-70 | [»] | |
4V6N | electron microscopy | 12.10 | A2 | 1-70 | [»] | |
4V6O | electron microscopy | 14.70 | B2 | 1-70 | [»] | |
4V6P | electron microscopy | 13.50 | B2 | 1-70 | [»] | |
4V6Q | electron microscopy | 11.50 | B2 | 1-70 | [»] | |
4V6R | electron microscopy | 11.50 | B2 | 1-70 | [»] | |
4V6S | electron microscopy | 13.10 | A2 | 1-70 | [»] | |
4V6V | electron microscopy | 9.80 | B4 | 1-70 | [»] | |
5AFI | electron microscopy | 2.90 | 6 | 1-70 | [»] | |
5AKA | electron microscopy | 5.70 | Z | 1-70 | [»] | |
5IQR | electron microscopy | 3.00 | a | 1-70 | [»] | |
5KCS | electron microscopy | 3.90 | 14 | 1-70 | [»] | |
5KPS | electron microscopy | 3.90 | 1 | 1-70 | [»] | |
5KPV | electron microscopy | 4.10 | Z | 1-70 | [»] | |
5KPW | electron microscopy | 3.90 | Z | 1-70 | [»] | |
5KPX | electron microscopy | 3.90 | Z | 1-70 | [»] | |
5L3P | electron microscopy | 3.70 | 4 | 1-70 | [»] | |
5LZA | electron microscopy | 3.60 | 6 | 1-66 | [»] | |
5LZB | electron microscopy | 5.30 | 6 | 1-66 | [»] | |
5LZC | electron microscopy | 4.80 | 6 | 1-66 | [»] | |
5LZD | electron microscopy | 3.40 | 6 | 1-66 | [»] | |
5LZE | electron microscopy | 3.50 | 6 | 1-66 | [»] | |
5LZF | electron microscopy | 4.60 | 6 | 1-66 | [»] | |
5MDV | electron microscopy | 2.97 | a | 1-70 | [»] | |
5MDW | electron microscopy | 3.06 | a | 1-70 | [»] | |
5MDY | electron microscopy | 3.35 | a | 1-70 | [»] | |
5MDZ | electron microscopy | 3.10 | a | 1-70 | [»] | |
5MGP | electron microscopy | 3.10 | 6 | 1-66 | [»] | |
5NP6 | electron microscopy | 3.60 | 4 | 1-66 | [»] | |
5NWY | electron microscopy | 2.93 | L | 1-70 | [»] | |
5O2R | electron microscopy | 3.40 | 6 | 1-66 | [»] | |
5U9F | electron microscopy | 3.20 | 29 | 1-70 | [»] | |
5U9G | electron microscopy | 3.20 | 29 | 1-70 | [»] | |
5UYK | electron microscopy | 3.90 | 29 | 1-66 | [»] | |
5UYL | electron microscopy | 3.60 | 29 | 1-66 | [»] | |
5UYM | electron microscopy | 3.20 | 29 | 1-66 | [»] | |
5UYN | electron microscopy | 4.00 | 29 | 1-66 | [»] | |
5UYP | electron microscopy | 3.90 | 29 | 1-66 | [»] | |
5UYQ | electron microscopy | 3.80 | 29 | 1-66 | [»] | |
5WDT | electron microscopy | 3.00 | 6 | 1-66 | [»] | |
5WE4 | electron microscopy | 3.10 | 6 | 1-66 | [»] | |
5WE6 | electron microscopy | 3.40 | 6 | 1-66 | [»] | |
5WFK | electron microscopy | 3.40 | 6 | 1-66 | [»] | |
6BU8 | electron microscopy | 3.50 | 29 | 1-66 | [»] | |
6BY1 | X-ray | 3.94 | C0/D0 | 10-48 | [»] | |
6C4I | electron microscopy | 3.24 | 1 | 1-70 | [»] | |
6ENF | electron microscopy | 3.20 | 6 | 1-66 | [»] | |
6ENJ | electron microscopy | 3.70 | 6 | 1-66 | [»] | |
6ENU | electron microscopy | 3.10 | 6 | 1-66 | [»] | |
6H4N | electron microscopy | 3.00 | 6 | 1-66 | [»] | |
6H58 | electron microscopy | 7.90 | 6/66 | 1-66 | [»] | |
6HRM | electron microscopy | 2.96 | a | 1-66 | [»] | |
6O9J | electron microscopy | 3.90 | Z | 1-70 | [»] | |
6ORE | electron microscopy | 2.90 | a | 1-66 | [»] | |
6OT3 | electron microscopy | 3.90 | a | 1-66 | [»] | |
6OUO | electron microscopy | 3.70 | a | 1-66 | [»] | |
6Q97 | electron microscopy | 3.90 | a | 1-66 | [»] | |
6Q98 | electron microscopy | 4.30 | a | 1-70 | [»] | |
6Q9A | electron microscopy | 3.70 | a | 1-66 | [»] | |
6SZS | electron microscopy | 3.06 | 5 | 1-70 | [»] | |
6TBV | electron microscopy | 2.70 | L311 | 1-70 | [»] | |
6TC3 | electron microscopy | 2.70 | L311 | 1-70 | [»] | |
6WNT | electron microscopy | 3.10 | A | 1-70 | [»] | |
6WNV | electron microscopy | 3.50 | A | 1-70 | [»] | |
6WNW | electron microscopy | 3.20 | A | 1-70 | [»] | |
6Y69 | electron microscopy | 2.86 | 6 | 1-66 | [»] | |
7AC7 | electron microscopy | 3.08 | a | 1-66 | [»] | |
7ACJ | electron microscopy | 3.20 | a | 1-65 | [»] | |
7ACR | electron microscopy | 3.44 | a | 1-65 | [»] | |
7B5K | electron microscopy | 2.90 | 5 | 1-66 | [»] | |
7BL2 | electron microscopy | 3.70 | b | 1-70 | [»] | |
7BL3 | electron microscopy | 3.50 | b | 1-70 | [»] | |
7BL4 | electron microscopy | 2.40 | d | 1-70 | [»] | |
7BL5 | electron microscopy | 3.30 | b | 1-70 | [»] | |
7BL6 | electron microscopy | 4.00 | d | 1-70 | [»] | |
7D6Z | electron microscopy | 3.40 | 6 | 1-70 | [»] | |
7K50 | electron microscopy | 3.40 | A | 1-66 | [»] | |
7K51 | electron microscopy | 3.50 | A | 1-66 | [»] | |
7K52 | electron microscopy | 3.40 | A | 1-66 | [»] | |
7K53 | electron microscopy | 3.20 | A | 1-66 | [»] | |
7K54 | electron microscopy | 3.20 | A | 1-66 | [»] | |
7K55 | electron microscopy | 3.30 | A | 1-66 | [»] | |
7LV0 | electron microscopy | 3.20 | A | 1-66 | [»] | |
7N1P | electron microscopy | 2.33 | Le | 1-70 | [»] | |
7N2C | electron microscopy | 2.72 | Le | 1-70 | [»] | |
7N2U | electron microscopy | 2.53 | Le | 1-70 | [»] | |
7N2V | electron microscopy | 2.54 | Le | 1-70 | [»] | |
7N30 | electron microscopy | 2.66 | Le | 1-70 | [»] | |
7N31 | electron microscopy | 2.69 | Le | 1-70 | [»] | |
7O19 | electron microscopy | 2.90 | BI | 1-70 | [»] | |
7O1A | electron microscopy | 2.40 | BI | 1-70 | [»] | |
7O1C | electron microscopy | 2.60 | BI | 1-70 | [»] | |
7P3K | electron microscopy | 2.90 | 4 | 1-70 | [»] | |
7PJV | electron microscopy | 3.10 | 6 | 1-70 | [»] | |
7PJY | electron microscopy | 3.10 | 6 | 1-70 | [»] | |
AlphaFoldDBi | P0A7M9 | |||||
SMRi | P0A7M9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261184, 44 interactors 852722, 7 interactors |
ComplexPortali | CPX-3807, 50S large ribosomal subunit |
IntActi | P0A7M9, 25 interactors |
STRINGi | 511145.b3936 |
PTM databases
iPTMneti | P0A7M9 |
Proteomic databases
jPOSTi | P0A7M9 |
PaxDbi | P0A7M9 |
PRIDEi | P0A7M9 |
Genome annotation databases
EnsemblBacteriai | AAC76918; AAC76918; b3936 BAE77374; BAE77374; BAE77374 |
GeneIDi | 66672156 948425 |
KEGGi | ecj:JW3907 eco:b3936 |
PATRICi | fig|1411691.4.peg.2769 |
Organism-specific databases
EchoBASEi | EB0882 |
Phylogenomic databases
eggNOGi | COG0254, Bacteria |
HOGENOMi | CLU_114306_4_3_6 |
InParanoidi | P0A7M9 |
OMAi | WYPDAKV |
PhylomeDBi | P0A7M9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10889-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7M9 |
PROi | PR:P0A7M9 |
Family and domain databases
Gene3Di | 4.10.830.30, 1 hit |
HAMAPi | MF_00501, Ribosomal_L31_1, 1 hit |
InterProi | View protein in InterPro IPR034704, L28p-like IPR002150, Ribosomal_L31 IPR027491, Ribosomal_L31_A IPR042105, Ribosomal_L31_sf |
PANTHERi | PTHR33280, PTHR33280, 1 hit |
Pfami | View protein in Pfam PF01197, Ribosomal_L31, 1 hit |
PRINTSi | PR01249, RIBOSOMALL31 |
SUPFAMi | SSF143800, SSF143800, 1 hit |
TIGRFAMsi | TIGR00105, L31, 1 hit |
PROSITEi | View protein in PROSITE PS01143, RIBOSOMAL_L31, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RL31_ECOLI | |
Accessioni | P0A7M9Primary (citable) accession number: P0A7M9 Secondary accession number(s): P02432, Q2M8N2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families