UniProtKB - P0A7I4 (RF3_ECOLI)
Protein
Peptide chain release factor RF3
Gene
prfC
Organism
Escherichia coli (strain K12)
Status
Functioni
Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 27 | GTPBy similarity | 8 | |
Nucleotide bindingi | 88 – 92 | GTPBy similarity | 5 | |
Nucleotide bindingi | 142 – 145 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: EcoCyc
- GTPase activity Source: EcoCyc
- GTP binding Source: UniProtKB-UniRule
- translation release factor activity, codon nonspecific Source: EcoCyc
- translation release factor activity, codon specific Source: UniProtKB-UniRule
GO - Biological processi
- regulation of translational termination Source: UniProtKB-UniRule
- translational termination Source: EcoCyc
Keywordsi
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12114-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide chain release factor RF3Short name: RF-3 |
Gene namesi | Name:prfC Synonyms:miaD, tos Ordered Locus Names:b4375, JW5873 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000210938 | 2 – 529 | Peptide chain release factor RF3Add BLAST | 528 |
Proteomic databases
jPOSTi | P0A7I4 |
PaxDbi | P0A7I4 |
PRIDEi | P0A7I4 |
Interactioni
Binary interactionsi
P0A7I4
With | #Exp. | IntAct |
---|---|---|
pfkA [P0A796] | 2 | EBI-556252,EBI-554405 |
Protein-protein interaction databases
BioGRIDi | 4260996, 56 interactors 853174, 1 interactor |
DIPi | DIP-39402N |
IntActi | P0A7I4, 5 interactors |
STRINGi | 511145.b4375 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7I4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7I4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 280 | tr-type GAdd BLAST | 270 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.Curated
Phylogenomic databases
eggNOGi | COG4108, Bacteria |
HOGENOMi | CLU_002794_2_1_6 |
InParanoidi | P0A7I4 |
PhylomeDBi | P0A7I4 |
Family and domain databases
CDDi | cd04169, RF3, 1 hit |
Gene3Di | 3.30.70.3280, 1 hit |
HAMAPi | MF_00072, Rel_fac_3, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR004548, PrfC IPR032090, RF3_C IPR038467, RF3_C_sf IPR041732, RF3_GTP-bd IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf |
PANTHERi | PTHR43556, PTHR43556, 1 hit |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF16658, RF3_C, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 1 hit |
TIGRFAMsi | TIGR00503, prfC, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A7I4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLSPYLQEV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QTAGTVKGRG
60 70 80 90 100
SNQHAKSDWM EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY
110 120 130 140 150
RTLTAVDCCL MVIDAAKGVE DRTRKLMEVT RLRDTPILTF MNKLDRDIRD
160 170 180 190 200
PMELLDEVEN ELKIGCAPIT WPIGCGKLFK GVYHLYKDET YLYQSGKGHT
210 220 230 240 250
IQEVRIVKGL NNPDLDAAVG EDLAQQLRDE LELVKGASNE FDKELFLAGE
260 270 280 290 300
ITPVFFGTAL GNFGVDHMLD GLVEWAPAPM PRQTDTRTVE ASEDKFTGFV
310 320 330 340 350
FKIQANMDPK HRDRVAFMRV VSGKYEKGMK LRQVRTAKDV VISDALTFMA
360 370 380 390 400
GDRSHVEEAY PGDILGLHNH GTIQIGDTFT QGEMMKFTGI PNFAPELFRR
410 420 430 440 450
IRLKDPLKQK QLLKGLVQLS EEGAVQVFRP ISNNDLIVGA VGVLQFDVVV
460 470 480 490 500
ARLKSEYNVE AVYESVNVAT ARWVECADAK KFEEFKRKNE SQLALDGGDN
510 520
LAYIATSMVN LRLAQERYPD VQFHQTREH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 315 | V → L in CAA81223 (PubMed:8016077).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D17724 Genomic DNA Translation: BAA04578.1 Z26313 Genomic DNA Translation: CAA81223.1 U14003 Genomic DNA Translation: AAA97271.1 U00096 Genomic DNA Translation: AAC77328.1 AP009048 Genomic DNA Translation: BAE78363.1 |
PIRi | I59305 |
RefSeqi | NP_418792.1, NC_000913.3 WP_000175940.1, NZ_SSUV01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC77328; AAC77328; b4375 BAE78363; BAE78363; BAE78363 |
GeneIDi | 948897 |
KEGGi | ecj:JW5873 eco:b4375 |
PATRICi | fig|1411691.4.peg.2313 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D17724 Genomic DNA Translation: BAA04578.1 Z26313 Genomic DNA Translation: CAA81223.1 U14003 Genomic DNA Translation: AAA97271.1 U00096 Genomic DNA Translation: AAC77328.1 AP009048 Genomic DNA Translation: BAE78363.1 |
PIRi | I59305 |
RefSeqi | NP_418792.1, NC_000913.3 WP_000175940.1, NZ_SSUV01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2H5E | X-ray | 2.80 | A/B | 1-529 | [»] | |
2O0F | electron microscopy | 15.50 | A | 1-529 | [»] | |
4V85 | X-ray | 3.20 | AW | 1-529 | [»] | |
4V89 | X-ray | 3.70 | AW | 1-529 | [»] | |
4V8O | X-ray | 3.80 | AY | 1-529 | [»] | |
6GXM | electron microscopy | 3.80 | w | 1-529 | [»] | |
6GXN | electron microscopy | 3.90 | w | 1-529 | [»] | |
6GXO | electron microscopy | 3.90 | w | 1-529 | [»] | |
6GXP | electron microscopy | 4.40 | w | 1-529 | [»] | |
SMRi | P0A7I4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260996, 56 interactors 853174, 1 interactor |
DIPi | DIP-39402N |
IntActi | P0A7I4, 5 interactors |
STRINGi | 511145.b4375 |
Proteomic databases
jPOSTi | P0A7I4 |
PaxDbi | P0A7I4 |
PRIDEi | P0A7I4 |
Genome annotation databases
EnsemblBacteriai | AAC77328; AAC77328; b4375 BAE78363; BAE78363; BAE78363 |
GeneIDi | 948897 |
KEGGi | ecj:JW5873 eco:b4375 |
PATRICi | fig|1411691.4.peg.2313 |
Organism-specific databases
EchoBASEi | EB2037 |
Phylogenomic databases
eggNOGi | COG4108, Bacteria |
HOGENOMi | CLU_002794_2_1_6 |
InParanoidi | P0A7I4 |
PhylomeDBi | P0A7I4 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12114-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7I4 |
PROi | PR:P0A7I4 |
Family and domain databases
CDDi | cd04169, RF3, 1 hit |
Gene3Di | 3.30.70.3280, 1 hit |
HAMAPi | MF_00072, Rel_fac_3, 1 hit |
InterProi | View protein in InterPro IPR035647, EFG_III/V IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR004548, PrfC IPR032090, RF3_C IPR038467, RF3_C_sf IPR041732, RF3_GTP-bd IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf |
PANTHERi | PTHR43556, PTHR43556, 1 hit |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF16658, RF3_C, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 1 hit |
TIGRFAMsi | TIGR00503, prfC, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RF3_ECOLI | |
Accessioni | P0A7I4Primary (citable) accession number: P0A7I4 Secondary accession number(s): P33998, Q2M5U3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families