UniProtKB - P0A7I0 (RF1_ECOLI)
Protein
Peptide chain release factor RF1
Gene
prfA
Organism
Escherichia coli (strain K12)
Status
Functioni
Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.
GO - Molecular functioni
- ribosome binding Source: EcoCyc
- translation release factor activity, codon specific Source: EcoCyc
GO - Biological processi
- translational termination Source: EcoCyc
Keywordsi
Biological process | Protein biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10761-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide chain release factor RF1Short name: RF-1 |
Gene namesi | Name:prfA Synonyms:sueB, uar Ordered Locus Names:b1211, JW1202 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 235 | Q → E or A: Loss of methylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000177668 | 1 – 360 | Peptide chain release factor RF1Add BLAST | 360 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 235 | N5-methylglutamine2 Publications | 1 |
Post-translational modificationi
Methylated by PrmC. Methylation increases the termination efficiency of RF1.3 Publications
Keywords - PTMi
MethylationProteomic databases
jPOSTi | P0A7I0 |
PaxDbi | P0A7I0 |
PRIDEi | P0A7I0 |
PTM databases
iPTMneti | P0A7I0 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260107, 60 interactors |
DIPi | DIP-35936N |
IntActi | P0A7I0, 13 interactors |
STRINGi | 511145.b1211 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7I0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7I0 |
Family & Domainsi
Sequence similaritiesi
Belongs to the prokaryotic/mitochondrial release factor family.Curated
Phylogenomic databases
eggNOGi | COG0216, Bacteria |
HOGENOMi | CLU_036856_0_1_6 |
InParanoidi | P0A7I0 |
PhylomeDBi | P0A7I0 |
Family and domain databases
HAMAPi | MF_00093, Rel_fac_1, 1 hit |
InterProi | View protein in InterPro IPR005139, PCRF IPR000352, Pep_chain_release_fac_I IPR004373, RF-1 |
Pfami | View protein in Pfam PF03462, PCRF, 1 hit PF00472, RF-1, 1 hit |
SMARTi | View protein in SMART SM00937, PCRF, 1 hit |
TIGRFAMsi | TIGR00019, prfA, 1 hit |
PROSITEi | View protein in PROSITE PS00745, RF_PROK_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7I0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPSIVAKLE ALHERHEEVQ ALLGDAQTIA DQERFRALSR EYAQLSDVSR
60 70 80 90 100
CFTDWQQVQE DIETAQMMLD DPEMREMAQD ELREAKEKSE QLEQQLQVLL
110 120 130 140 150
LPKDPDDERN AFLEVRAGTG GDEAALFAGD LFRMYSRYAE ARRWRVEIMS
160 170 180 190 200
ASEGEHGGYK EIIAKISGDG VYGRLKFESG GHRVQRVPAT ESQGRIHTSA
210 220 230 240 250
CTVAVMPELP DAELPDINPA DLRIDTFRSS GAGGQHVNTT DSAIRITHLP
260 270 280 290 300
TGIVVECQDE RSQHKNKAKA LSVLGARIHA AEMAKRQQAE ASTRRNLLGS
310 320 330 340 350
GDRSDRNRTY NFPQGRVTDH RINLTLYRLD EVMEGKLDML IEPIIQEHQA
360
DQLAALSEQE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 – 91 | PEMRE…EKSEQ → LRYLLYLRLFVVLFRHVVGD in M30785 (PubMed:2684779).CuratedAdd BLAST | 20 | |
Sequence conflicti | 211 | D → E in AAC43437 (PubMed:7543480).Curated | 1 | |
Sequence conflicti | 241 | D → G in AAA24519 (PubMed:3889910).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11519 Genomic DNA Translation: AAA24519.1 U18555 Genomic DNA Translation: AAC43437.1 U00096 Genomic DNA Translation: AAC74295.1 AP009048 Genomic DNA Translation: BAA36069.1 M30785 Genomic DNA No translation available. M25323 Genomic DNA Translation: AAA23955.1 D28567 Genomic DNA Translation: BAA05914.1 |
PIRi | H64867, FCECR1 |
RefSeqi | NP_415729.1, NC_000913.3 WP_000804726.1, NZ_STEB01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC74295; AAC74295; b1211 BAA36069; BAA36069; BAA36069 |
GeneIDi | 58460430 949002 |
KEGGi | ecj:JW1202 eco:b1211 |
PATRICi | fig|1411691.4.peg.1073 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11519 Genomic DNA Translation: AAA24519.1 U18555 Genomic DNA Translation: AAC43437.1 U00096 Genomic DNA Translation: AAC74295.1 AP009048 Genomic DNA Translation: BAA36069.1 M30785 Genomic DNA No translation available. M25323 Genomic DNA Translation: AAA23955.1 D28567 Genomic DNA Translation: BAA05914.1 |
PIRi | H64867, FCECR1 |
RefSeqi | NP_415729.1, NC_000913.3 WP_000804726.1, NZ_STEB01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2B3T | X-ray | 3.10 | B | 1-360 | [»] | |
5J30 | X-ray | 3.20 | QY/XY | 1-360 | [»] | |
5J3C | X-ray | 3.04 | QY/XY | 1-360 | [»] | |
5J4D | X-ray | 3.10 | JA/OC | 1-360 | [»] | |
5O2R | electron microscopy | 3.40 | v | 110-351 | [»] | |
6GWT | electron microscopy | 3.80 | v | 104-351 | [»] | |
6GXM | electron microscopy | 3.80 | v | 104-351 | [»] | |
6GXN | electron microscopy | 3.90 | v | 2-351 | [»] | |
6GXO | electron microscopy | 3.90 | v | 104-351 | [»] | |
6ORL | electron microscopy | 3.50 | A | 100-356 | [»] | |
SMRi | P0A7I0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260107, 60 interactors |
DIPi | DIP-35936N |
IntActi | P0A7I0, 13 interactors |
STRINGi | 511145.b1211 |
PTM databases
iPTMneti | P0A7I0 |
Proteomic databases
jPOSTi | P0A7I0 |
PaxDbi | P0A7I0 |
PRIDEi | P0A7I0 |
Genome annotation databases
EnsemblBacteriai | AAC74295; AAC74295; b1211 BAA36069; BAA36069; BAA36069 |
GeneIDi | 58460430 949002 |
KEGGi | ecj:JW1202 eco:b1211 |
PATRICi | fig|1411691.4.peg.1073 |
Organism-specific databases
EchoBASEi | EB0754 |
Phylogenomic databases
eggNOGi | COG0216, Bacteria |
HOGENOMi | CLU_036856_0_1_6 |
InParanoidi | P0A7I0 |
PhylomeDBi | P0A7I0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10761-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7I0 |
PROi | PR:P0A7I0 |
Family and domain databases
HAMAPi | MF_00093, Rel_fac_1, 1 hit |
InterProi | View protein in InterPro IPR005139, PCRF IPR000352, Pep_chain_release_fac_I IPR004373, RF-1 |
Pfami | View protein in Pfam PF03462, PCRF, 1 hit PF00472, RF-1, 1 hit |
SMARTi | View protein in SMART SM00937, PCRF, 1 hit |
TIGRFAMsi | TIGR00019, prfA, 1 hit |
PROSITEi | View protein in PROSITE PS00745, RF_PROK_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RF1_ECOLI | |
Accessioni | P0A7I0Primary (citable) accession number: P0A7I0 Secondary accession number(s): P07011, P77340 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families