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UniProtKB - P0A7I0 (RF1_ECOLI)

Entry version 130 (17 Jun 2020)
Sequence version 1 (07 Jun 2005)
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Protein

Peptide chain release factor RF1

Gene

prfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG10761-MONOMER
ECOL316407:JW1202-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Peptide chain release factor RF1
Short name:
RF-1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:prfA
Synonyms:sueB, uar
Ordered Locus Names:b1211, JW1202
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi235Q → E or A: Loss of methylation. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001776681 – 360Peptide chain release factor RF1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei235N5-methylglutamine2 Publications1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF1.3 Publications

Keywords - PTMi

Methylation

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0A7I0

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0A7I0

PRoteomics IDEntifications database

More...PRIDEi		P0A7I0

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P0A7I0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260107, 60 interactors

Database of interacting proteins

More...DIPi		DIP-35936N

Protein interaction database and analysis system

More...IntActi		P0A7I0, 13 interactors

STRING: functional protein association networks

More...STRINGi		511145.b1211

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1360
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0A7I0

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P0A7I0

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the prokaryotic/mitochondrial release factor family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG4105C8K Bacteria
COG0216 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_036856_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0A7I0

KEGG Orthology (KO)

More...KOi		K02835

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0A7I0

Family and domain databases

HAMAP database of protein families

More...HAMAPi		MF_00093 Rel_fac_1, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR005139 PCRF
IPR000352 Pep_chain_release_fac_I
IPR004373 PrfA

Pfam protein domain database

More...Pfami		View protein in Pfam
PF03462 PCRF, 1 hit
PF00472 RF-1, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00937 PCRF, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00019 prfA, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00745 RF_PROK_I, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0A7I0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPSIVAKLE ALHERHEEVQ ALLGDAQTIA DQERFRALSR EYAQLSDVSR 
        60         70         80         90        100
CFTDWQQVQE DIETAQMMLD DPEMREMAQD ELREAKEKSE QLEQQLQVLL 
       110        120        130        140        150
LPKDPDDERN AFLEVRAGTG GDEAALFAGD LFRMYSRYAE ARRWRVEIMS 
       160        170        180        190        200
ASEGEHGGYK EIIAKISGDG VYGRLKFESG GHRVQRVPAT ESQGRIHTSA 
       210        220        230        240        250
CTVAVMPELP DAELPDINPA DLRIDTFRSS GAGGQHVNTT DSAIRITHLP 
       260        270        280        290        300
TGIVVECQDE RSQHKNKAKA LSVLGARIHA AEMAKRQQAE ASTRRNLLGS 
       310        320        330        340        350
GDRSDRNRTY NFPQGRVTDH RINLTLYRLD EVMEGKLDML IEPIIQEHQA 
       360
DQLAALSEQE
Length:360
Mass (Da):40,517
Last modified:June 7, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i47B3B77705A71954
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti72 – 91PEMRE…EKSEQ → LRYLLYLRLFVVLFRHVVGD in M30785 (PubMed:2684779).CuratedAdd BLAST20
Sequence conflicti211D → E in AAC43437 (PubMed:7543480).Curated1
Sequence conflicti241D → G in AAA24519 (PubMed:3889910).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M11519 Genomic DNA Translation: AAA24519.1
U18555 Genomic DNA Translation: AAC43437.1
U00096 Genomic DNA Translation: AAC74295.1
AP009048 Genomic DNA Translation: BAA36069.1
M30785 Genomic DNA No translation available.
M25323 Genomic DNA Translation: AAA23955.1
D28567 Genomic DNA Translation: BAA05914.1

Protein sequence database of the Protein Information Resource

More...PIRi		H64867 FCECR1

NCBI Reference Sequences

More...RefSeqi		NP_415729.1, NC_000913.3
WP_000804726.1, NZ_STEB01000023.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC74295; AAC74295; b1211
BAA36069; BAA36069; BAA36069

Database of genes from NCBI RefSeq genomes

More...GeneIDi		949002

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW1202
eco:b1211

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1073

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11519 Genomic DNA Translation: AAA24519.1
U18555 Genomic DNA Translation: AAC43437.1
U00096 Genomic DNA Translation: AAC74295.1
AP009048 Genomic DNA Translation: BAA36069.1
M30785 Genomic DNA No translation available.
M25323 Genomic DNA Translation: AAA23955.1
D28567 Genomic DNA Translation: BAA05914.1
PIRiH64867 FCECR1
RefSeqiNP_415729.1, NC_000913.3
WP_000804726.1, NZ_STEB01000023.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B3TX-ray3.10B1-360[»]
5J30X-ray3.20QY/XY1-360[»]
5J3CX-ray3.04QY/XY1-360[»]
5J4DX-ray3.10JA/OC1-360[»]
5O2Relectron microscopy3.40v110-351[»]
6GWTelectron microscopy3.80v104-351[»]
6GXMelectron microscopy3.80v104-351[»]
6GXNelectron microscopy3.90v2-351[»]
6GXOelectron microscopy3.90v104-351[»]
6ORLelectron microscopy3.50A100-356[»]
SMRiP0A7I0
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4260107, 60 interactors
DIPiDIP-35936N
IntActiP0A7I0, 13 interactors
STRINGi511145.b1211

PTM databases

iPTMnetiP0A7I0

Proteomic databases

jPOSTiP0A7I0
PaxDbiP0A7I0
PRIDEiP0A7I0

Genome annotation databases

EnsemblBacteriaiAAC74295; AAC74295; b1211
BAA36069; BAA36069; BAA36069
GeneIDi949002
KEGGiecj:JW1202
eco:b1211
PATRICifig|1411691.4.peg.1073

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0754

Phylogenomic databases

eggNOGiENOG4105C8K Bacteria
COG0216 LUCA
HOGENOMiCLU_036856_0_1_6
InParanoidiP0A7I0
KOiK02835
PhylomeDBiP0A7I0

Enzyme and pathway databases

BioCyciEcoCyc:EG10761-MONOMER
ECOL316407:JW1202-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A7I0

Protein Ontology

More...PROi		PR:P0A7I0

Family and domain databases

HAMAPiMF_00093 Rel_fac_1, 1 hit
InterProiView protein in InterPro
IPR005139 PCRF
IPR000352 Pep_chain_release_fac_I
IPR004373 PrfA
PfamiView protein in Pfam
PF03462 PCRF, 1 hit
PF00472 RF-1, 1 hit
SMARTiView protein in SMART
SM00937 PCRF, 1 hit
TIGRFAMsiTIGR00019 prfA, 1 hit
PROSITEiView protein in PROSITE
PS00745 RF_PROK_I, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRF1_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A7I0
Secondary accession number(s): P07011, P77340
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2005
Last modified: June 17, 2020
This is version 130 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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