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Protein

DNA replication and repair protein RecF

Gene

recF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-UniRule
  • DNA synthesis involved in DNA repair Source: EcoliWiki
  • double-strand break repair Source: GO_Central
  • response to radiation Source: EcoCyc
  • response to UV Source: EcoliWiki
  • SOS response Source: UniProtKB-UniRule

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair, DNA replication, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10828-MONOMER
MetaCyc:EG10828-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecF
Gene namesi
Name:recF
Synonyms:uvrF
Ordered Locus Names:b3700, JW3677
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10828 recF

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36K → R: Weakly active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001964142 – 357DNA replication and repair protein RecFAdd BLAST356

Proteomic databases

PaxDbiP0A7H0
PRIDEiP0A7H0

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F54EBI-556839,EBI-543750

Protein-protein interaction databases

BioGridi4261540, 217 interactors
DIPiDIP-36037N
IntActiP0A7H0, 13 interactors
STRINGi316385.ECDH10B_3886

Structurei

3D structure databases

ProteinModelPortaliP0A7H0
SMRiP0A7H0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.Curated

Phylogenomic databases

eggNOGiENOG4105C3X Bacteria
COG1195 LUCA
HOGENOMiHOG000269560
InParanoidiP0A7H0
KOiK03629
PhylomeDBiP0A7H0

Family and domain databases

HAMAPiMF_00365 RecF, 1 hit
InterProiView protein in InterPro
IPR001238 DNA-binding_RecF
IPR018078 DNA-binding_RecF_CS
IPR027417 P-loop_NTPase
IPR003395 RecF/RecN/SMC_N
PANTHERiPTHR32182:SF0 PTHR32182:SF0, 1 hit
PfamiView protein in Pfam
PF02463 SMC_N, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00611 recf, 1 hit
PROSITEiView protein in PROSITE
PS00617 RECF_1, 1 hit
PS00618 RECF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7H0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR
60 70 80 90 100
AFRSLQIGRV IRHEQEAFVL HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT
110 120 130 140 150
DGHKVAELAH LMPMQLITPE GFTLLNGGPK YRRAFLDWGC FHNEPGFFTA
160 170 180 190 200
WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE LIPLAEQIST WRAEYSAGIA
210 220 230 240 250
ADMADTCKQF LPEFSLTFSF QRGWEKETEY AEVLERNFER DRQLTYTAHG
260 270 280 290 300
PHKADLRIRA DGAPVEDTLS RGQLKLLMCA LRLAQGEFLT RESGRRCLYL
310 320 330 340 350
IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV

EKGKITD
Length:357
Mass (Da):40,514
Last modified:January 23, 2007 - v2
Checksum:iECB395B963C5DDB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02179 Genomic DNA Translation: AAA24511.1
X04341 Genomic DNA Translation: CAA27870.1
L10328 Genomic DNA Translation: AAA62051.1
U00096 Genomic DNA Translation: AAC76723.1
AP009048 Genomic DNA Translation: BAE77594.1
PIRiA03547 RQECF
RefSeqiNP_418155.1, NC_000913.3
WP_000060112.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76723; AAC76723; b3700
BAE77594; BAE77594; BAE77594
GeneIDi948209
KEGGiecj:JW3677
eco:b3700
PATRICifig|1411691.4.peg.3003

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02179 Genomic DNA Translation: AAA24511.1
X04341 Genomic DNA Translation: CAA27870.1
L10328 Genomic DNA Translation: AAA62051.1
U00096 Genomic DNA Translation: AAC76723.1
AP009048 Genomic DNA Translation: BAE77594.1
PIRiA03547 RQECF
RefSeqiNP_418155.1, NC_000913.3
WP_000060112.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP0A7H0
SMRiP0A7H0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261540, 217 interactors
DIPiDIP-36037N
IntActiP0A7H0, 13 interactors
STRINGi316385.ECDH10B_3886

Proteomic databases

PaxDbiP0A7H0
PRIDEiP0A7H0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76723; AAC76723; b3700
BAE77594; BAE77594; BAE77594
GeneIDi948209
KEGGiecj:JW3677
eco:b3700
PATRICifig|1411691.4.peg.3003

Organism-specific databases

EchoBASEiEB0821
EcoGeneiEG10828 recF

Phylogenomic databases

eggNOGiENOG4105C3X Bacteria
COG1195 LUCA
HOGENOMiHOG000269560
InParanoidiP0A7H0
KOiK03629
PhylomeDBiP0A7H0

Enzyme and pathway databases

BioCyciEcoCyc:EG10828-MONOMER
MetaCyc:EG10828-MONOMER

Miscellaneous databases

PROiPR:P0A7H0

Family and domain databases

HAMAPiMF_00365 RecF, 1 hit
InterProiView protein in InterPro
IPR001238 DNA-binding_RecF
IPR018078 DNA-binding_RecF_CS
IPR027417 P-loop_NTPase
IPR003395 RecF/RecN/SMC_N
PANTHERiPTHR32182:SF0 PTHR32182:SF0, 1 hit
PfamiView protein in Pfam
PF02463 SMC_N, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00611 recf, 1 hit
PROSITEiView protein in PROSITE
PS00617 RECF_1, 1 hit
PS00618 RECF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRECF_ECOLI
AccessioniPrimary (citable) accession number: P0A7H0
Secondary accession number(s): P03016, Q2M812
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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