UniProtKB - P0A7G2 (RBFA_ECOLI)
Protein
30S ribosome-binding factor
Gene
rbfA
Organism
Escherichia coli (strain K12)
Status
Functioni
One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S subunit. Essential for efficient processing of pre-16S rRNA (PubMed:9422595, PubMed:12963368, PubMed:12628255). Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Probably interacts with the 5'-terminal helix region of 16S rRNA (PubMed:7535280). Has affinity for free ribosomal 30S subunits but not for 70S ribosomes (PubMed:7535280, PubMed:12963368). Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation (PubMed:7535280). Overexpression decreases the lag time following cold-shock by about half, leading to faster adaptation and increased protein synthesis (PubMed:8898389). Overexpression also partially suppresses a rimM deletion mutant and partially rescues its 16S rRNA processing deficiency (PubMed:9422595). Its function overlaps that of Era in ribosome biogenesis (PubMed:16825789). A number of RbfA mutants suppress RsgA/YjeQ deletions, in all cases less RbfA is bound to the 30S ribosome (PubMed:21102555). Released from 30S ribosomes by RsgA; stimulates the ribosome-associated GTPase activity of RsgA (PubMed:21102555).7 Publications
GO - Molecular functioni
- ribosomal small subunit binding Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoCyc
- maturation of SSU-rRNA Source: EcoliWiki
- response to cold Source: EcoCyc
- ribosome biogenesis Source: GO_Central
Keywordsi
Biological process | Ribosome biogenesis, Stress response |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11178-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 30S ribosome-binding factor1 PublicationAlternative name(s): Protein P15B1 Publication Ribosome-binding factor A1 PublicationUniRule annotation Short name: RbfA1 Publication |
Gene namesi | Name:rbfA1 PublicationUniRule annotation Synonyms:P15B1 Publication, yhbB Ordered Locus Names:b3167, JW3136 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Severe growth defect at 26 and 30 degrees Celsius, with poor growth at 37 and 42 degrees; has decreased amounts of 70S ribosomes and polysomes and increased amounts of 30S and 50S subunits (PubMed:7535280). Results in a constitutive induction of the cold-shock response; increased cold-shock and ribosomal protein synthesis continues in the presence of general translation inhibition (PubMed:8898389). Less efficient processing of 5' end of 16S rRNA, increased levels of precursor 17S rRNA; effect is more pronounced in cold-shocked cells (PubMed:9422595, PubMed:12963368). Both phenotypes are partially suppressed by overexpression of Era and completely suppressed by overexpression of Era-delta 'Ala-40-Gly-49' mutation (PubMed:12753192). Double rbfA-rsgA deletion mutants have the same phenotype as single mutants (PubMed:21102555).6 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | R → H: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 30 | P → L: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 77 | G → D: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 84 | G → E: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 100 | D → G: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 109 – 133 | Missing : Complements small colony and slow growth phenotype of the deletion mutant at 15 degrees Celsius, growth is not quite wild-type. Restores pre-16S rRNA processing but does not suppress cold-sensitive C23U 16S rRNA mutation. 2 PublicationsAdd BLAST | 25 | |
Mutagenesisi | 112 – 133 | LVTSV…DSKED → GDQRGQT: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 PublicationAdd BLAST | 22 | |
Mutagenesisi | 120 | D → N: Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 Publication | 1 | |
Mutagenesisi | 122 – 133 | Missing : Suppresses growth defects and restores maturation of the 30S subunit of an rsgA deletion. Decreased affinity for 30S ribosomes. 1 PublicationAdd BLAST | 12 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000102658 | 2 – 133 | 30S ribosome-binding factorAdd BLAST | 132 |
Proteomic databases
jPOSTi | P0A7G2 |
PaxDbi | P0A7G2 |
PRIDEi | P0A7G2 |
2D gel databases
SWISS-2DPAGEi | P0A7G2 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 4262435, 190 interactors |
IntActi | P0A7G2, 2 interactors |
STRINGi | 511145.b3167 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7G2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7G2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 109 – 133 | Required for stable 30S ribosomal association in vitro1 PublicationAdd BLAST | 25 |
Domaini
The C-terminus (residues 109-133) is required for stable association with the 30S ribosomal subunit in vitro.1 Publication
Sequence similaritiesi
Belongs to the RbfA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0858, Bacteria |
HOGENOMi | CLU_089475_5_0_6 |
InParanoidi | P0A7G2 |
PhylomeDBi | P0A7G2 |
Family and domain databases
Gene3Di | 3.30.300.20, 1 hit |
HAMAPi | MF_00003, RbfA, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR000238, RbfA IPR023799, RbfA_dom_sf IPR020053, Ribosome-bd_factorA_CS |
PANTHERi | PTHR33515, PTHR33515, 1 hit |
Pfami | View protein in Pfam PF02033, RBFA, 1 hit |
SUPFAMi | SSF89919, SSF89919, 1 hit |
TIGRFAMsi | TIGR00082, rbfA, 1 hit |
PROSITEi | View protein in PROSITE PS01319, RBFA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A7G2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA
60 70 80 90 100
KVYVTFLNDK DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD
110 120 130
NSLVEGMRMS NLVTSVVKHD EERRVNPDDS KED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13270 Genomic DNA Translation: CAA31634.1 X13775 Genomic DNA Translation: CAA32020.1 U18997 Genomic DNA Translation: AAA57970.1 U00096 Genomic DNA Translation: AAC76201.1 AP009048 Genomic DNA Translation: BAE77213.1 |
PIRi | S01915, Q9EC15 |
RefSeqi | NP_417636.1, NC_000913.3 WP_001040205.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76201; AAC76201; b3167 BAE77213; BAE77213; BAE77213 |
GeneIDi | 58462835 947685 |
KEGGi | ecj:JW3136 eco:b3167 |
PATRICi | fig|1411691.4.peg.3563 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13270 Genomic DNA Translation: CAA31634.1 X13775 Genomic DNA Translation: CAA32020.1 U18997 Genomic DNA Translation: AAA57970.1 U00096 Genomic DNA Translation: AAC76201.1 AP009048 Genomic DNA Translation: BAE77213.1 |
PIRi | S01915, Q9EC15 |
RefSeqi | NP_417636.1, NC_000913.3 WP_001040205.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KKG | NMR | - | A | 1-108 | [»] | |
SMRi | P0A7G2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262435, 190 interactors |
IntActi | P0A7G2, 2 interactors |
STRINGi | 511145.b3167 |
2D gel databases
SWISS-2DPAGEi | P0A7G2 |
Proteomic databases
jPOSTi | P0A7G2 |
PaxDbi | P0A7G2 |
PRIDEi | P0A7G2 |
Genome annotation databases
EnsemblBacteriai | AAC76201; AAC76201; b3167 BAE77213; BAE77213; BAE77213 |
GeneIDi | 58462835 947685 |
KEGGi | ecj:JW3136 eco:b3167 |
PATRICi | fig|1411691.4.peg.3563 |
Organism-specific databases
EchoBASEi | EB1165 |
Phylogenomic databases
eggNOGi | COG0858, Bacteria |
HOGENOMi | CLU_089475_5_0_6 |
InParanoidi | P0A7G2 |
PhylomeDBi | P0A7G2 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11178-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7G2 |
PROi | PR:P0A7G2 |
Family and domain databases
Gene3Di | 3.30.300.20, 1 hit |
HAMAPi | MF_00003, RbfA, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR000238, RbfA IPR023799, RbfA_dom_sf IPR020053, Ribosome-bd_factorA_CS |
PANTHERi | PTHR33515, PTHR33515, 1 hit |
Pfami | View protein in Pfam PF02033, RBFA, 1 hit |
SUPFAMi | SSF89919, SSF89919, 1 hit |
TIGRFAMsi | TIGR00082, rbfA, 1 hit |
PROSITEi | View protein in PROSITE PS01319, RBFA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBFA_ECOLI | |
Accessioni | P0A7G2Primary (citable) accession number: P0A7G2 Secondary accession number(s): P09170, Q2M943 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families