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Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).

Miscellaneous

This reaction follows a completely ordered sequential bi-ter kinetic mechanism, with binding of preQ1-tRNATyr followed by AdoMet to form the ternary complex. Products are then released in the following order: adenine, methionine, and oQ-tRNATyr.

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.1 Publication

Enzyme regulationi

Inhibited by S-adenosylhomocysteine, adenosylornithine and products.

Kineticsi

  1. KM=98 µM for S-adenosylmethionine1 Publication
  2. KM=1.9 µM for 7-(aminomethyl)-7-deazaguanosine1 Publication

    Pathwayi: tRNA-queuosine biosynthesis

    This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.
    View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

    GO - Molecular functioni

    GO - Biological processi

    • queuosine biosynthetic process Source: EcoCyc
    • response to radiation Source: EcoCyc
    • tRNA wobble guanine modification Source: EcoCyc

    Keywordsi

    Molecular functionTransferase
    Biological processQueuosine biosynthesis
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10812-MONOMER
    MetaCyc:EG10812-MONOMER
    SABIO-RKiP0A7F9
    UniPathwayiUPA00392

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC:2.4.99.17)
    Alternative name(s):
    Queuosine biosynthesis protein QueA
    Gene namesi
    Name:queA
    Ordered Locus Names:b0405, JW0395
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10812 queA

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001654001 – 356S-adenosylmethionine:tRNA ribosyltransferase-isomeraseAdd BLAST356

    Proteomic databases

    PaxDbiP0A7F9
    PRIDEiP0A7F9

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi4262056, 62 interactors
    DIPiDIP-47972N
    IntActiP0A7F9, 5 interactors
    STRINGi316385.ECDH10B_0361

    Structurei

    3D structure databases

    ProteinModelPortaliP0A7F9
    SMRiP0A7F9
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the QueA family.Curated

    Phylogenomic databases

    eggNOGiENOG4105E2N Bacteria
    COG0809 LUCA
    HOGENOMiHOG000004401
    InParanoidiP0A7F9
    KOiK07568
    OMAiYSYGDGM
    PhylomeDBiP0A7F9

    Family and domain databases

    HAMAPiMF_00113 QueA, 1 hit
    InterProiView protein in InterPro
    IPR003699 QueA
    IPR036100 QueA_sf
    PANTHERiPTHR30307 PTHR30307, 1 hit
    PfamiView protein in Pfam
    PF02547 Queuosine_synth, 1 hit
    SUPFAMiSSF111337 SSF111337, 1 hit
    TIGRFAMsiTIGR00113 queA, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A7F9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVTDFSFEL PESLIAHYPM PERSSCRLLS LDGPTGALTH GTFTDLLDKL
    60 70 80 90 100
    NPGDLLVFNN TRVIPARLFG RKASGGKIEV LVERMLDDKR ILAHIRASKA
    110 120 130 140 150
    PKPGAELLLG DDESINATMT ARHGALFEVE FNDERSVLDI LNSIGHMPLP
    160 170 180 190 200
    PYIDRPDEDA DRELYQTVYS EKPGAVAAPT AGLHFDEPLL EKLRAKGVEM
    210 220 230 240 250
    AFVTLHVGAG TFQPVRVDTI EDHIMHSEYA EVPQDVVDAV LAAKARGNRV
    260 270 280 290 300
    IAVGTTSVRS LESAAQAAKN DLIEPFFDDT QIFIYPGFQY KVVDALVTNF
    310 320 330 340 350
    HLPESTLIML VSAFAGYQHT MNAYKAAVEE KYRFFSYGDA MFITYNPQAI

    NERVGE
    Length:356
    Mass (Da):39,431
    Last modified:June 7, 2005 - v1
    Checksum:i47F7F0090812DCF3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M37702 Genomic DNA Translation: AAA16114.1
    U82664 Genomic DNA Translation: AAB40161.1
    U00096 Genomic DNA Translation: AAC73508.1
    AP009048 Genomic DNA Translation: BAE76185.1
    PIRiB38530
    RefSeqiNP_414939.1, NC_000913.3
    WP_001266503.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73508; AAC73508; b0405
    BAE76185; BAE76185; BAE76185
    GeneIDi944905
    KEGGiecj:JW0395
    eco:b0405
    PATRICifig|1411691.4.peg.1872

    Similar proteinsi

    Entry informationi

    Entry nameiQUEA_ECOLI
    AccessioniPrimary (citable) accession number: P0A7F9
    Secondary accession number(s): P21516, Q2MC21
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: March 28, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

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