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UniProtKB - P0A7F3 (PYRI_ECOLI)
Protein
Aspartate carbamoyltransferase regulatory chain
Gene
pyrI
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in allosteric regulation of aspartate carbamoyltransferase.
Cofactori
Zn2+Note: Binds 1 zinc ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 109 | Zinc | 1 | |
Metal bindingi | 114 | Zinc | 1 | |
Metal bindingi | 138 | Zinc | 1 | |
Metal bindingi | 141 | Zinc | 1 |
GO - Molecular functioni
- zinc ion binding Source: EcoCyc
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: ComplexPortal
- pyrimidine nucleotide biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Biological process | Pyrimidine biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:ASPCARBREG-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate carbamoyltransferase regulatory chain |
Gene namesi | Name:pyrI Ordered Locus Names:b4244, JW4203 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- aspartate carbamoyltransferase complex Source: ComplexPortal
- cytoplasm Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000142303 | 2 – 153 | Aspartate carbamoyltransferase regulatory chainAdd BLAST | 152 |
Proteomic databases
jPOSTi | P0A7F3 |
PaxDbi | P0A7F3 |
PRIDEi | P0A7F3 |
2D gel databases
SWISS-2DPAGEi | P0A7F3 |
Interactioni
Subunit structurei
Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).
1 PublicationBinary interactionsi
P0A7F3
With | #Exp. | IntAct |
---|---|---|
pyrB [P0A786] | 18 | EBI-906630,EBI-906620 |
Protein-protein interaction databases
BioGRIDi | 4263117, 11 interactors 853052, 1 interactor |
ComplexPortali | CPX-3091, Aspartate carbamoyltransferase complex |
DIPi | DIP-35088N |
IntActi | P0A7F3, 2 interactors |
MINTi | P0A7F3 |
STRINGi | 511145.b4244 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7F3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7F3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PyrI family.Curated
Phylogenomic databases
eggNOGi | COG1781, Bacteria |
HOGENOMi | CLU_128576_0_0_6 |
InParanoidi | P0A7F3 |
PhylomeDBi | P0A7F3 |
Family and domain databases
Gene3Di | 3.30.70.140, 1 hit |
HAMAPi | MF_00002, Asp_carb_tr_reg, 1 hit |
InterProi | View protein in InterPro IPR020545, Asp_carbamoyltransf_reg_N IPR002801, Asp_carbamoylTrfase_reg IPR020542, Asp_carbamoyltrfase_reg_C IPR036792, Asp_carbatrfase_reg_C_sf IPR036793, Asp_carbatrfase_reg_N_sf |
PANTHERi | PTHR35805, PTHR35805, 1 hit |
Pfami | View protein in Pfam PF01948, PyrI, 1 hit PF02748, PyrI_C, 1 hit |
SUPFAMi | SSF54893, SSF54893, 1 hit SSF57825, SSF57825, 1 hit |
TIGRFAMsi | TIGR00240, ATCase_reg, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A7F3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTHDNKLQVE AIKRGTVIDH IPAQIGFKLL SLFKLTETDQ RITIGLNLPS
60 70 80 90 100
GEMGRKDLIK IENTFLSEDQ VDQLALYAPQ ATVNRIDNYE VVGKSRPSLP
110 120 130 140 150
ERIDNVLVCP NSNCISHAEP VSSSFAVRKR ANDIALKCKY CEKEFSHNVV
LAN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01472 Genomic DNA Translation: AAA24477.1 U14003 Genomic DNA Translation: AAA97141.1 U00096 Genomic DNA Translation: AAC77201.1 AP009048 Genomic DNA Translation: BAE78243.1 M28578 Genomic DNA Translation: AAA24487.1 |
PIRi | A93985, DTECR |
RefSeqi | NP_418665.1, NC_000913.3 WP_000148581.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77201; AAC77201; b4244 BAE78243; BAE78243; BAE78243 |
GeneIDi | 67414869 948763 |
KEGGi | ecj:JW4203 eco:b4244 |
PATRICi | fig|1411691.4.peg.2457 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01472 Genomic DNA Translation: AAA24477.1 U14003 Genomic DNA Translation: AAA97141.1 U00096 Genomic DNA Translation: AAC77201.1 AP009048 Genomic DNA Translation: BAE78243.1 M28578 Genomic DNA Translation: AAA24487.1 |
PIRi | A93985, DTECR |
RefSeqi | NP_418665.1, NC_000913.3 WP_000148581.1, NZ_STEB01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ACM | X-ray | 2.80 | B/D | 1-153 | [»] | |
1AT1 | X-ray | 2.80 | B/D | 1-153 | [»] | |
1D09 | X-ray | 2.10 | B/D | 1-153 | [»] | |
1EZZ | X-ray | 2.70 | B/D | 1-153 | [»] | |
1F1B | X-ray | 2.30 | B/D | 1-153 | [»] | |
1I5O | X-ray | 2.80 | B/D | 1-153 | [»] | |
1NBE | X-ray | 2.60 | B/D | 1-153 | [»] | |
1Q95 | X-ray | 2.46 | G/H/I/J/K/L | 1-153 | [»] | |
1R0B | X-ray | 2.90 | G/H/I/J/K/L | 1-153 | [»] | |
1R0C | X-ray | 2.37 | B/H | 1-153 | [»] | |
1RAA | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAB | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAC | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAD | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAE | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAF | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAG | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAH | X-ray | 2.50 | B/D | 1-153 | [»] | |
1RAI | X-ray | 2.50 | B/D | 1-153 | [»] | |
1SKU | X-ray | 2.60 | B/D | 1-153 | [»] | |
1TTH | X-ray | 2.80 | B/D | 1-153 | [»] | |
1TU0 | X-ray | 2.55 | B/D | 1-153 | [»] | |
1TUG | X-ray | 2.10 | B/D | 1-153 | [»] | |
1XJW | X-ray | 2.71 | B/D | 1-153 | [»] | |
1ZA1 | X-ray | 2.20 | B/D | 1-153 | [»] | |
1ZA2 | X-ray | 2.50 | B/D | 1-153 | [»] | |
2A0F | X-ray | 2.90 | B/D | 1-153 | [»] | |
2AIR | X-ray | 2.00 | B/H | 1-153 | [»] | |
2AT1 | X-ray | 2.80 | B/D | 1-153 | [»] | |
2ATC | X-ray | 3.00 | B | 1-153 | [»] | |
2FZC | X-ray | 2.10 | B/D | 1-153 | [»] | |
2FZG | X-ray | 2.25 | B/D | 1-153 | [»] | |
2FZK | X-ray | 2.50 | B/D | 1-153 | [»] | |
2H3E | X-ray | 2.30 | B/D | 1-153 | [»] | |
2HSE | X-ray | 2.60 | B/D | 1-153 | [»] | |
2IPO | X-ray | 2.60 | B/D | 1-153 | [»] | |
2QG9 | X-ray | 2.70 | B/D | 1-153 | [»] | |
2QGF | X-ray | 2.20 | B/D | 1-153 | [»] | |
3AT1 | X-ray | 2.80 | B/D | 1-153 | [»] | |
3D7S | X-ray | 2.80 | B/D | 1-153 | [»] | |
3MPU | X-ray | 2.86 | B/D/F | 1-153 | [»] | |
4AT1 | X-ray | 2.60 | B/D | 1-153 | [»] | |
4E2F | X-ray | 2.80 | B/D/F/H/J/L | 1-153 | [»] | |
4F04 | X-ray | 2.30 | B/D | 1-153 | [»] | |
4FYV | X-ray | 2.10 | B/D | 1-153 | [»] | |
4FYW | X-ray | 2.10 | B/D | 1-153 | [»] | |
4FYX | X-ray | 2.09 | B/D | 1-153 | [»] | |
4FYY | X-ray | 1.94 | B/D | 1-153 | [»] | |
5AT1 | X-ray | 2.60 | B/D | 1-153 | [»] | |
6AT1 | X-ray | 2.60 | B/D | 1-153 | [»] | |
6KJ8 | X-ray | 3.01 | B/D/F | 1-153 | [»] | |
6KJA | X-ray | 3.06 | B/D/F | 1-153 | [»] | |
6KJB | X-ray | 2.06 | B | 1-153 | [»] | |
7AT1 | X-ray | 2.80 | B/D | 1-153 | [»] | |
8AT1 | X-ray | 2.80 | B/D | 1-153 | [»] | |
8ATC | X-ray | 2.50 | B/D | 1-153 | [»] | |
9ATC | X-ray | 2.40 | B | 8-153 | [»] | |
SMRi | P0A7F3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263117, 11 interactors 853052, 1 interactor |
ComplexPortali | CPX-3091, Aspartate carbamoyltransferase complex |
DIPi | DIP-35088N |
IntActi | P0A7F3, 2 interactors |
MINTi | P0A7F3 |
STRINGi | 511145.b4244 |
2D gel databases
SWISS-2DPAGEi | P0A7F3 |
Proteomic databases
jPOSTi | P0A7F3 |
PaxDbi | P0A7F3 |
PRIDEi | P0A7F3 |
Genome annotation databases
EnsemblBacteriai | AAC77201; AAC77201; b4244 BAE78243; BAE78243; BAE78243 |
GeneIDi | 67414869 948763 |
KEGGi | ecj:JW4203 eco:b4244 |
PATRICi | fig|1411691.4.peg.2457 |
Organism-specific databases
EchoBASEi | EB0804 |
Phylogenomic databases
eggNOGi | COG1781, Bacteria |
HOGENOMi | CLU_128576_0_0_6 |
InParanoidi | P0A7F3 |
PhylomeDBi | P0A7F3 |
Enzyme and pathway databases
BioCyci | EcoCyc:ASPCARBREG-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7F3 |
PROi | PR:P0A7F3 |
Family and domain databases
Gene3Di | 3.30.70.140, 1 hit |
HAMAPi | MF_00002, Asp_carb_tr_reg, 1 hit |
InterProi | View protein in InterPro IPR020545, Asp_carbamoyltransf_reg_N IPR002801, Asp_carbamoylTrfase_reg IPR020542, Asp_carbamoyltrfase_reg_C IPR036792, Asp_carbatrfase_reg_C_sf IPR036793, Asp_carbatrfase_reg_N_sf |
PANTHERi | PTHR35805, PTHR35805, 1 hit |
Pfami | View protein in Pfam PF01948, PyrI, 1 hit PF02748, PyrI_C, 1 hit |
SUPFAMi | SSF54893, SSF54893, 1 hit SSF57825, SSF57825, 1 hit |
TIGRFAMsi | TIGR00240, ATCase_reg, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYRI_ECOLI | |
Accessioni | P0A7F3Primary (citable) accession number: P0A7F3 Secondary accession number(s): P00478, Q2M663 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families