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UniProtKB - P0A7E3 (PYRE_ECOLI)
Protein
Orotate phosphoribosyltransferase
Gene
pyrE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
UniRule annotation1 PublicationCatalytic activityi
- diphosphate + orotidine 5'-phosphate = 5-phospho-α-D-ribose 1-diphosphate + orotateUniRule annotationEC:2.4.2.10UniRule annotation
Cofactori
Mg2+UniRule annotation
Activity regulationi
Inhibited by sulfate and phosphate ions.1 Publication
: UMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 26 | 5-phosphoribose 1-diphosphateUniRule annotation | 1 | |
Binding sitei | 99 | 5-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1 Publication | 1 | |
Binding sitei | 100 | 5-phosphoribose 1-diphosphateUniRule annotation1 Publication | 1 | |
Binding sitei | 103 | 5-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1 Publication | 1 | |
Binding sitei | 105 | 5-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 128 | OrotateUniRule annotation | 1 | |
Binding sitei | 156 | OrotateUniRule annotation | 1 |
GO - Molecular functioni
- magnesium ion binding Source: EcoCyc
- orotate phosphoribosyltransferase activity Source: EcoCyc
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: EcoCyc
- 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
- pyrimidine nucleotide biosynthetic process Source: EcoliWiki
- pyrimidine ribonucleoside biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Pyrimidine biosynthesis |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:OROPRIBTRANS-MONOMER |
UniPathwayi | UPA00070;UER00119 |
Names & Taxonomyi
Protein namesi | Recommended name: Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)Short name: OPRTUniRule annotation Short name: OPRTaseUniRule annotation |
Gene namesi | Name:pyrE1 Publication Ordered Locus Names:b3642, JW3617 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000110694 | 2 – 213 | Orotate phosphoribosyltransferaseAdd BLAST | 212 |
Proteomic databases
jPOSTi | P0A7E3 |
PaxDbi | P0A7E3 |
PRIDEi | P0A7E3 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259572, 15 interactors |
IntActi | P0A7E3, 1 interactor |
STRINGi | 511145.b3642 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7E3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7E3 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 35 | Orotate bindingUniRule annotation | 2 | |
Regioni | 72 – 73 | 5-phosphoribose 1-diphosphate bindingUniRule annotation1 Publication | 2 | |
Regioni | 124 – 132 | 5-phosphoribose 1-diphosphate bindingUniRule annotation | 9 |
Sequence similaritiesi
Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0461, Bacteria |
HOGENOMi | CLU_074878_0_1_6 |
InParanoidi | P0A7E3 |
PhylomeDBi | P0A7E3 |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
HAMAPi | MF_01208, PyrE, 1 hit |
InterProi | View protein in InterPro IPR023031, OPRT IPR004467, Or_phspho_trans_dom IPR000836, PRibTrfase_dom IPR029057, PRTase-like |
Pfami | View protein in Pfam PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR00336, pyrE, 1 hit |
PROSITEi | View protein in PROSITE PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A7E3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPYQRQFIE FALSKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG
60 70 80 90 100
RFYAEALVDS GIEFDLLFGP AYKGIPIATT TAVALAEHHD LDLPYCFNRK
110 120 130 140 150
EAKDHGEGGN LVGSALQGRV MLVDDVITAG TAIRESMEII QANGATLAGV
160 170 180 190 200
LISLDRQERG RGEISAIQEV ERDYNCKVIS IITLKDLIAY LEEKPEMAEH
210
LAAVKAYREE FGV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 133 | Missing (PubMed:6349999).Curated | 1 | |
Sequence conflicti | 133 | Missing (PubMed:6207018).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00781 Genomic DNA Translation: CAA25358.1 V01578 Genomic DNA Translation: CAA24899.1 L10328 Genomic DNA Translation: AAA61995.1 U00096 Genomic DNA Translation: AAC76666.1 AP009048 Genomic DNA Translation: BAE77650.1 |
PIRi | D65165, XJEC |
RefSeqi | NP_418099.1, NC_000913.3 WP_000806177.1, NZ_STEB01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC76666; AAC76666; b3642 BAE77650; BAE77650; BAE77650 |
GeneIDi | 60902666 948157 |
KEGGi | ecj:JW3617 eco:b3642 |
PATRICi | fig|1411691.4.peg.3064 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00781 Genomic DNA Translation: CAA25358.1 V01578 Genomic DNA Translation: CAA24899.1 L10328 Genomic DNA Translation: AAA61995.1 U00096 Genomic DNA Translation: AAC76666.1 AP009048 Genomic DNA Translation: BAE77650.1 |
PIRi | D65165, XJEC |
RefSeqi | NP_418099.1, NC_000913.3 WP_000806177.1, NZ_STEB01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ORO | X-ray | 2.40 | A/B | 1-213 | [»] | |
6TAI | X-ray | 1.55 | AAA/BBB | 1-213 | [»] | |
6TAJ | X-ray | 1.60 | AAA/BBB | 1-213 | [»] | |
6TAK | X-ray | 1.25 | AAA/BBB | 1-213 | [»] | |
SMRi | P0A7E3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259572, 15 interactors |
IntActi | P0A7E3, 1 interactor |
STRINGi | 511145.b3642 |
Proteomic databases
jPOSTi | P0A7E3 |
PaxDbi | P0A7E3 |
PRIDEi | P0A7E3 |
Genome annotation databases
EnsemblBacteriai | AAC76666; AAC76666; b3642 BAE77650; BAE77650; BAE77650 |
GeneIDi | 60902666 948157 |
KEGGi | ecj:JW3617 eco:b3642 |
PATRICi | fig|1411691.4.peg.3064 |
Organism-specific databases
EchoBASEi | EB0801 |
Phylogenomic databases
eggNOGi | COG0461, Bacteria |
HOGENOMi | CLU_074878_0_1_6 |
InParanoidi | P0A7E3 |
PhylomeDBi | P0A7E3 |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00119 |
BioCyci | EcoCyc:OROPRIBTRANS-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A7E3 |
PROi | PR:P0A7E3 |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
HAMAPi | MF_01208, PyrE, 1 hit |
InterProi | View protein in InterPro IPR023031, OPRT IPR004467, Or_phspho_trans_dom IPR000836, PRibTrfase_dom IPR029057, PRTase-like |
Pfami | View protein in Pfam PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR00336, pyrE, 1 hit |
PROSITEi | View protein in PROSITE PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYRE_ECOLI | |
Accessioni | P0A7E3Primary (citable) accession number: P0A7E3 Secondary accession number(s): P00495, Q2M7V6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families