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UniProtKB - P0A7D7 (PUR7_ECOLI)
Protein
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Gene
purC
Organism
Escherichia coli (strain K12)
Status
Functioni
Converts 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) to 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide (SAICAR).
1 PublicationCatalytic activityi
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H+ + phosphate1 PublicationEC:6.3.2.61 Publication
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate. This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- guanosine tetraphosphate binding Source: EcoCyc
- phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: EcoCyc
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: GO_Central
- cobalamin biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:SAICARSYN-MONOMER |
BRENDAi | 6.3.2.6, 2026 |
UniPathwayi | UPA00074;UER00131 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.61 Publication)Alternative name(s): SAICAR synthetase |
Gene namesi | Name:purC Ordered Locus Names:b2476, JW2461 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100825 | 1 – 237 | Phosphoribosylaminoimidazole-succinocarboxamide synthaseAdd BLAST | 237 |
Proteomic databases
jPOSTi | P0A7D7 |
PaxDbi | P0A7D7 |
PRIDEi | P0A7D7 |
2D gel databases
SWISS-2DPAGEi | P0A7D7 |
Interactioni
Subunit structurei
Homotrimer.
Protein-protein interaction databases
BioGRIDi | 4261983, 44 interactors |
DIPi | DIP-35900N |
IntActi | P0A7D7, 17 interactors |
STRINGi | 511145.b2476 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A7D7 |
SMRi | P0A7D7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7D7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SAICAR synthetase family.Curated
Phylogenomic databases
eggNOGi | COG0152, Bacteria |
HOGENOMi | CLU_061495_2_1_6 |
InParanoidi | P0A7D7 |
OMAi | EFCYKND |
PhylomeDBi | P0A7D7 |
Family and domain databases
CDDi | cd01415, SAICAR_synt_PurC, 1 hit |
HAMAPi | MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR028923, SAICAR_synt/ADE2_N IPR033934, SAICAR_synt_PurC IPR001636, SAICAR_synth IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF01259, SAICAR_synt, 1 hit |
TIGRFAMsi | TIGR00081, purC, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7D7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQKQAELYRG KAKTVYSTEN PDLLVLEFRN DTSAGDGARI EQFDRKGMVN
60 70 80 90 100
NKFNYFIMSK LAEAGIPTQM ERLLSDTECL VKKLDMVPVE CVVRNRAAGS
110 120 130 140 150
LVKRLGIEEG IELNPPLFDL FLKNDAMHDP MVNESYCETF GWVSKENLAR
160 170 180 190 200
MKELTYKAND VLKKLFDDAG LILVDFKLEF GLYKGEVVLG DEFSPDGSRL
210 220 230
WDKETLEKMD KDRFRQSLGG LIEAYEAVAR RLGVQLD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33928 Genomic DNA Translation: AAA24448.1 U00096 Genomic DNA Translation: AAC75529.1 AP009048 Genomic DNA Translation: BAA16353.1 X57402 Genomic DNA Translation: CAA40662.1 |
PIRi | C36146 |
RefSeqi | NP_416971.1, NC_000913.3 WP_001295467.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75529; AAC75529; b2476 BAA16353; BAA16353; BAA16353 |
GeneIDi | 67416868 946957 |
KEGGi | ecj:JW2461 eco:b2476 |
PATRICi | fig|1411691.4.peg.4263 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33928 Genomic DNA Translation: AAA24448.1 U00096 Genomic DNA Translation: AAC75529.1 AP009048 Genomic DNA Translation: BAA16353.1 X57402 Genomic DNA Translation: CAA40662.1 |
PIRi | C36146 |
RefSeqi | NP_416971.1, NC_000913.3 WP_001295467.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GQR | X-ray | 2.00 | A/B | 1-237 | [»] | |
2GQS | X-ray | 2.05 | A/B | 1-237 | [»] | |
AlphaFoldDBi | P0A7D7 | |||||
SMRi | P0A7D7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261983, 44 interactors |
DIPi | DIP-35900N |
IntActi | P0A7D7, 17 interactors |
STRINGi | 511145.b2476 |
2D gel databases
SWISS-2DPAGEi | P0A7D7 |
Proteomic databases
jPOSTi | P0A7D7 |
PaxDbi | P0A7D7 |
PRIDEi | P0A7D7 |
Genome annotation databases
EnsemblBacteriai | AAC75529; AAC75529; b2476 BAA16353; BAA16353; BAA16353 |
GeneIDi | 67416868 946957 |
KEGGi | ecj:JW2461 eco:b2476 |
PATRICi | fig|1411691.4.peg.4263 |
Organism-specific databases
EchoBASEi | EB0784 |
Phylogenomic databases
eggNOGi | COG0152, Bacteria |
HOGENOMi | CLU_061495_2_1_6 |
InParanoidi | P0A7D7 |
OMAi | EFCYKND |
PhylomeDBi | P0A7D7 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00131 |
BioCyci | EcoCyc:SAICARSYN-MONOMER |
BRENDAi | 6.3.2.6, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A7D7 |
PROi | PR:P0A7D7 |
Family and domain databases
CDDi | cd01415, SAICAR_synt_PurC, 1 hit |
HAMAPi | MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR028923, SAICAR_synt/ADE2_N IPR033934, SAICAR_synt_PurC IPR001636, SAICAR_synth IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF01259, SAICAR_synt, 1 hit |
TIGRFAMsi | TIGR00081, purC, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR7_ECOLI | |
Accessioni | P0A7D7Primary (citable) accession number: P0A7D7 Secondary accession number(s): P21155 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families