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Protein

Peptidyl-tRNA hydrolase

Gene

pth

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death.

Catalytic activityi

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.UniRule annotation

GO - Molecular functioni

  • aminoacyl-tRNA hydrolase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10785-MONOMER
MetaCyc:EG10785-MONOMER
BRENDAi3.1.1.29 2026
SABIO-RKiP0A7D1

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-tRNA hydrolaseUniRule annotation (EC:3.1.1.29UniRule annotation)
Short name:
PTHUniRule annotation
Gene namesi
Name:pthUniRule annotation
Ordered Locus Names:b1204, JW1195
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10785 pth

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101G → D: Pth(ts) mutants synthesize thermosensitive PTH and die at 42 degrees Celsius from a defect in protein synthesis. 1
Mutagenesisi134R → H: Rap mutants do not support vegetative growth of bacteriophage lambda and die upon transcription of lambda DNA bar sites. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001877331 – 194Peptidyl-tRNA hydrolaseAdd BLAST194

Proteomic databases

EPDiP0A7D1
PaxDbiP0A7D1
PRIDEiP0A7D1

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262870, 54 interactors
DIPiDIP-35932N
IntActiP0A7D1, 16 interactors
STRINGi316385.ECDH10B_1257

Structurei

Secondary structure

1194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A7D1
SMRiP0A7D1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7D1

Family & Domainsi

Sequence similaritiesi

Belongs to the PTH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZPD Bacteria
COG0193 LUCA
HOGENOMiHOG000004796
InParanoidiP0A7D1
KOiK01056
PhylomeDBiP0A7D1

Family and domain databases

CDDicd00462 PTH, 1 hit
Gene3Di3.40.50.1470, 1 hit
HAMAPiMF_00083 Pept_tRNA_hydro_bact, 1 hit
InterProiView protein in InterPro
IPR001328 Pept_tRNA_hydro
IPR018171 Pept_tRNA_hydro_CS
IPR036416 Pept_tRNA_hydro_sf
PANTHERiPTHR17224 PTHR17224, 1 hit
PfamiView protein in Pfam
PF01195 Pept_tRNA_hydro, 1 hit
SUPFAMiSSF53178 SSF53178, 1 hit
TIGRFAMsiTIGR00447 pth, 1 hit
PROSITEiView protein in PROSITE
PS01195 PEPT_TRNA_HYDROL_1, 1 hit
PS01196 PEPT_TRNA_HYDROL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A7D1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTIKLIVGLA NPGAEYAATR HNAGAWFVDL LAERLRAPLR EEAKFFGYTS
60 70 80 90 100
RVTLGGEDVR LLVPTTFMNL SGKAVAAMAS FFRINPDEIL VAHDELDLPP
110 120 130 140 150
GVAKFKLGGG HGGHNGLKDI ISKLGNNPNF HRLRIGIGHP GDKNKVVGFV
160 170 180 190
LGKPPVSEQK LIDEAIDEAA RCTEMWFTDG LTKATNRLHA FKAQ
Length:194
Mass (Da):21,082
Last modified:June 7, 2005 - v1
Checksum:iA28A69A63F2DD530
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61941 Genomic DNA Translation: CAA43945.1
U00096 Genomic DNA Translation: AAC74288.1
AP009048 Genomic DNA Translation: BAA36062.1
PIRiS16753
RefSeqiNP_415722.1, NC_000913.3
WP_000152933.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74288; AAC74288; b1204
BAA36062; BAA36062; BAA36062
GeneIDi945765
KEGGiecj:JW1195
eco:b1204
PATRICifig|1411691.4.peg.1080

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61941 Genomic DNA Translation: CAA43945.1
U00096 Genomic DNA Translation: AAC74288.1
AP009048 Genomic DNA Translation: BAA36062.1
PIRiS16753
RefSeqiNP_415722.1, NC_000913.3
WP_000152933.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PTHX-ray1.20A2-194[»]
3VJRX-ray2.40A/C1-194[»]
ProteinModelPortaliP0A7D1
SMRiP0A7D1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262870, 54 interactors
DIPiDIP-35932N
IntActiP0A7D1, 16 interactors
STRINGi316385.ECDH10B_1257

Proteomic databases

EPDiP0A7D1
PaxDbiP0A7D1
PRIDEiP0A7D1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74288; AAC74288; b1204
BAA36062; BAA36062; BAA36062
GeneIDi945765
KEGGiecj:JW1195
eco:b1204
PATRICifig|1411691.4.peg.1080

Organism-specific databases

EchoBASEiEB0778
EcoGeneiEG10785 pth

Phylogenomic databases

eggNOGiENOG4108ZPD Bacteria
COG0193 LUCA
HOGENOMiHOG000004796
InParanoidiP0A7D1
KOiK01056
PhylomeDBiP0A7D1

Enzyme and pathway databases

BioCyciEcoCyc:EG10785-MONOMER
MetaCyc:EG10785-MONOMER
BRENDAi3.1.1.29 2026
SABIO-RKiP0A7D1

Miscellaneous databases

EvolutionaryTraceiP0A7D1
PROiPR:P0A7D1

Family and domain databases

CDDicd00462 PTH, 1 hit
Gene3Di3.40.50.1470, 1 hit
HAMAPiMF_00083 Pept_tRNA_hydro_bact, 1 hit
InterProiView protein in InterPro
IPR001328 Pept_tRNA_hydro
IPR018171 Pept_tRNA_hydro_CS
IPR036416 Pept_tRNA_hydro_sf
PANTHERiPTHR17224 PTHR17224, 1 hit
PfamiView protein in Pfam
PF01195 Pept_tRNA_hydro, 1 hit
SUPFAMiSSF53178 SSF53178, 1 hit
TIGRFAMsiTIGR00447 pth, 1 hit
PROSITEiView protein in PROSITE
PS01195 PEPT_TRNA_HYDROL_1, 1 hit
PS01196 PEPT_TRNA_HYDROL_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTH_ECOLI
AccessioniPrimary (citable) accession number: P0A7D1
Secondary accession number(s): P23932
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 7, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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