UniProtKB - P0A7C2 (LEXA_ECOLI)
Protein
LexA repressor
Gene
lexA
Organism
Escherichia coli (strain K12)
Status
Functioni
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).UniRule annotation4 Publications
Catalytic activityi
- Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation EC:3.4.21.88
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 119 | For autocatalytic cleavage activityUniRule annotation | 1 | |
Active sitei | 156 | For autocatalytic cleavage activityUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 28 – 48 | H-T-H motifUniRule annotationAdd BLAST | 21 |
GO - Molecular functioni
- DNA binding Source: EcoliWiki
- DNA-binding transcription repressor activity Source: CollecTF
- identical protein binding Source: IntAct
- serine-type endopeptidase activity Source: UniProtKB-UniRule
- transcription regulatory region sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- DNA repair Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-UniRule
- negative regulation of transcription, DNA-templated Source: EcoliWiki
- SOS response Source: EcoCyc
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Hydrolase, Repressor |
Biological process | DNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00205 |
BRENDAi | 3.4.21.88, 2026 |
Protein family/group databases
MEROPSi | S24.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lexAUniRule annotation Synonyms:exrA, spr, tsl, umuA Ordered Locus Names:b4043, JW4003 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
- protein-DNA complex Source: CollecTF
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170031 | 1 – 202 | LexA repressorAdd BLAST | 202 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 84 – 85 | Cleavage; by autolysis | 2 |
Keywords - PTMi
Autocatalytic cleavageProteomic databases
jPOSTi | P0A7C2 |
PaxDbi | P0A7C2 |
PRIDEi | P0A7C2 |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
Hide detailsP0A7C2
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-553416,EBI-553416 |
rpsE [P0A7W1] | 2 | EBI-553416,EBI-543949 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261718, 11 interactors 852838, 8 interactors |
DIPi | DIP-51082N |
IntActi | P0A7C2, 11 interactors |
STRINGi | 511145.b4043 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A7C2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7C2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S24 family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1974, Bacteria |
HOGENOMi | CLU_066192_45_3_6 |
InParanoidi | P0A7C2 |
PhylomeDBi | P0A7C2 |
Family and domain databases
CDDi | cd06529, S24_LexA-like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00015, LexA, 1 hit |
InterProi | View protein in InterPro IPR006200, LexA IPR039418, LexA-like IPR036286, LexA/Signal_pep-like_sf IPR006199, LexA_DNA-bd_dom IPR006197, Peptidase_S24_LexA IPR015927, Peptidase_S24_S26A/B/C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01726, LexA_DNA_bind, 1 hit PF00717, Peptidase_S24, 1 hit |
PRINTSi | PR00726, LEXASERPTASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR00498, lexA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7C2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL
60 70 80 90 100
ARKGVIEIVS GASRGIRLLQ EEEEGLPLVG RVAAGEPLLA QQHIEGHYQV
110 120 130 140 150
DPSLFKPNAD FLLRVSGMSM KDIGIMDGDL LAVHKTQDVR NGQVVVARID
160 170 180 190 200
DEVTVKRLKK QGNKVELLPE NSEFKPIVVD LRQQSFTIEG LAVGVIRNGD
WL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 85 | G → D in lexA3, resistant to cleavage. Increased sensitivity to hydroxyurea. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01643 Genomic DNA Translation: AAA24067.1 U00006 Genomic DNA Translation: AAC43137.1 U00096 Genomic DNA Translation: AAC77013.1 AP009048 Genomic DNA Translation: BAE78045.1 L02362 Genomic DNA Translation: AAA24068.1 |
PIRi | A90808, ILEC |
RefSeqi | NP_418467.1, NC_000913.3 WP_000646078.1, NZ_STEB01000022.1 |
Genome annotation databases
EnsemblBacteriai | AAC77013; AAC77013; b4043 BAE78045; BAE78045; BAE78045 |
GeneIDi | 52078664 948544 |
KEGGi | ecj:JW4003 eco:b4043 |
PATRICi | fig|511145.12.peg.4160 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01643 Genomic DNA Translation: AAA24067.1 U00006 Genomic DNA Translation: AAC43137.1 U00096 Genomic DNA Translation: AAC77013.1 AP009048 Genomic DNA Translation: BAE78045.1 L02362 Genomic DNA Translation: AAA24068.1 |
PIRi | A90808, ILEC |
RefSeqi | NP_418467.1, NC_000913.3 WP_000646078.1, NZ_STEB01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JHC | X-ray | 2.00 | A | 68-202 | [»] | |
1JHE | X-ray | 2.50 | A/B | 68-202 | [»] | |
1JHF | X-ray | 1.80 | A/B | 1-202 | [»] | |
1JHH | X-ray | 2.10 | A/B | 1-202 | [»] | |
1LEA | NMR | - | A | 1-84 | [»] | |
1LEB | NMR | - | A | 1-84 | [»] | |
1MVD | model | - | A/B | 1-202 | [»] | |
1QAA | model | - | A/B | 1-202 | [»] | |
3JSO | X-ray | 2.29 | A/B | 1-202 | [»] | |
3JSP | X-ray | 2.90 | A/B | 1-202 | [»] | |
3K3R | X-ray | 3.20 | E/F | 1-202 | [»] | |
SMRi | P0A7C2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261718, 11 interactors 852838, 8 interactors |
DIPi | DIP-51082N |
IntActi | P0A7C2, 11 interactors |
STRINGi | 511145.b4043 |
Protein family/group databases
MEROPSi | S24.001 |
Proteomic databases
jPOSTi | P0A7C2 |
PaxDbi | P0A7C2 |
PRIDEi | P0A7C2 |
Genome annotation databases
EnsemblBacteriai | AAC77013; AAC77013; b4043 BAE78045; BAE78045; BAE78045 |
GeneIDi | 52078664 948544 |
KEGGi | ecj:JW4003 eco:b4043 |
PATRICi | fig|511145.12.peg.4160 |
Organism-specific databases
EchoBASEi | EB0528 |
Phylogenomic databases
eggNOGi | COG1974, Bacteria |
HOGENOMi | CLU_066192_45_3_6 |
InParanoidi | P0A7C2 |
PhylomeDBi | P0A7C2 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00205 |
BRENDAi | 3.4.21.88, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A7C2 |
PROi | PR:P0A7C2 |
Gene expression databases
CollecTFi | EXPREG_000007a0 |
Family and domain databases
CDDi | cd06529, S24_LexA-like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00015, LexA, 1 hit |
InterProi | View protein in InterPro IPR006200, LexA IPR039418, LexA-like IPR036286, LexA/Signal_pep-like_sf IPR006199, LexA_DNA-bd_dom IPR006197, Peptidase_S24_LexA IPR015927, Peptidase_S24_S26A/B/C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01726, LexA_DNA_bind, 1 hit PF00717, Peptidase_S24, 1 hit |
PRINTSi | PR00726, LEXASERPTASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR00498, lexA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEXA_ECOLI | |
Accessioni | P0A7C2Primary (citable) accession number: P0A7C2 Secondary accession number(s): P03033, Q2M6R1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries