UniProtKB - P0A7B8 (HSLV_ECOLI)
ATP-dependent protease subunit HslV
hslV
Functioni
Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. The complex has been shown to be involved in the specific degradation of heat shock induced transcription factors such as RpoH and SulA. In addition, small hydrophobic peptides are also hydrolyzed by HslV. HslV has weak protease activity even in the absence of HslU, but this activity is induced more than 100-fold in the presence of HslU. HslU recognizes protein substrates and unfolds these before guiding them to HslV for hydrolysis. HslV is not believed to degrade folded proteins.
UniRule annotation7 PublicationsCaution
Catalytic activityi
- ATP-dependent cleavage of peptide bonds with broad specificity.UniRule annotation5 Publications EC:3.4.25.2
Activity regulationi
Kineticsi
- KM=5.2 µM for Arc-MYL-st11 (at 37 degrees Celsius)1 Publication
Temperature dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | UniRule annotation1 Publication | 1 | |
Metal bindingi | 157 | Sodium; via carbonyl oxygen | 1 | |
Metal bindingi | 160 | Sodium; via carbonyl oxygen | 1 | |
Metal bindingi | 163 | Sodium; via carbonyl oxygen | 1 |
GO - Molecular functioni
- ATP binding Source: CAFA
- identical protein binding Source: IntAct
- magnesium ion binding Source: CAFA
- protein domain specific binding Source: CAFA
- threonine-type endopeptidase activity Source: EcoCyc
GO - Biological processi
- protein denaturation Source: ComplexPortal
- proteolysis Source: CAFA
- proteolysis involved in cellular protein catabolic process Source: EcoCyc
- response to heat Source: EcoliWiki
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase, Protease, Threonine protease |
Biological process | Stress response |
Ligand | Metal-binding, Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11676-MONOMER |
BRENDAi | 3.4.25.2, 2026 |
Protein family/group databases
MEROPSi | T01.006 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent protease subunit HslVUniRule annotation (EC:3.4.25.2UniRule annotation)Alternative name(s): Heat shock protein HslVUniRule annotation |
Gene namesi | Name:hslVUniRule annotation Synonyms:htpO, yiiC Ordered Locus Names:b3932, JW3903 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
- HslUV protease complex Source: CAFA
Other locations
- proteasome core complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | T → S: 80% reduced protease activity in the absence of HslU. Almost no effect in the presence of HslU. 1 Publication | 1 | |
Mutagenesisi | 2 | T → V: No protease activity. 1 Publication | 1 | |
Mutagenesisi | 3 | T → S or V: 80% reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 6 | S → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 104 | S → A: 50% reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 125 | S → A: Almost no protease activity. 1 Publication | 1 | |
Mutagenesisi | 144 | S → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 160 | C → A or S: No protease activity. Cannot form complexes with HslU. 1 Publication | 1 | |
Mutagenesisi | 173 | S → A: Almost no protease activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000148105 | 2 – 176 | ATP-dependent protease subunit HslVAdd BLAST | 175 |
Proteomic databases
jPOSTi | P0A7B8 |
PaxDbi | P0A7B8 |
PRIDEi | P0A7B8 |
2D gel databases
SWISS-2DPAGEi | P0A7B8 |
Expressioni
Inductioni
Interactioni
Subunit structurei
A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.
UniRule annotation1 PublicationBinary interactionsi
P0A7B8
With | #Exp. | IntAct |
---|---|---|
hslU [P0A6H5] | 16 | EBI-552265,EBI-369317 |
itself | 6 | EBI-552265,EBI-552265 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein domain specific binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4261781, 287 interactors |
ComplexPortali | CPX-2104, HslUV protease complex |
DIPi | DIP-35866N |
IntActi | P0A7B8, 33 interactors |
STRINGi | 511145.b3932 |
Structurei
Secondary structure
3D structure databases
SMRi | P0A7B8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7B8 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG5405, Bacteria |
HOGENOMi | CLU_093872_1_0_6 |
InParanoidi | P0A7B8 |
PhylomeDBi | P0A7B8 |
Family and domain databases
CDDi | cd01913, protease_HslV, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
HAMAPi | MF_00248, HslV, 1 hit |
InterProi | View protein in InterPro IPR022281, ATP-dep_Prtase_HsIV_su IPR029055, Ntn_hydrolases_N IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR32194, PTHR32194, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PIRSFi | PIRSF039093, HslV, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR03692, ATP_dep_HslV, 1 hit |
PROSITEi | View protein in PROSITE PS51476, PROTEASOME_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTTIVSVRRN GHVVIAGDGQ ATLGNTVMKG NVKKVRRLYN DKVIAGFAGG
60 70 80 90 100
TADAFTLFEL FERKLEMHQG HLVKAAVELA KDWRTDRMLR KLEALLAVAD
110 120 130 140 150
ETASLIITGN GDVVQPENDL IAIGSGGPYA QAAARALLEN TELSAREIAE
160 170
KALDIAGDIC IYTNHFHTIE ELSYKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19201 Genomic DNA Translation: AAB03064.1 U00096 Genomic DNA Translation: AAC76914.1 AP009048 Genomic DNA Translation: BAE77378.1 D89965 mRNA Translation: BAA14040.1 Sequence problems. L14281 Genomic DNA No translation available. |
PIRi | JT0760 |
RefSeqi | NP_418367.1, NC_000913.3 WP_000208242.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76914; AAC76914; b3932 BAE77378; BAE77378; BAE77378 |
GeneIDi | 67417555 948429 |
KEGGi | ecj:JW3903 eco:b3932 |
PATRICi | fig|1411691.4.peg.2773 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19201 Genomic DNA Translation: AAB03064.1 U00096 Genomic DNA Translation: AAC76914.1 AP009048 Genomic DNA Translation: BAE77378.1 D89965 mRNA Translation: BAA14040.1 Sequence problems. L14281 Genomic DNA No translation available. |
PIRi | JT0760 |
RefSeqi | NP_418367.1, NC_000913.3 WP_000208242.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E94 | X-ray | 2.80 | A/B/C/D | 2-176 | [»] | |
1G4A | X-ray | 3.00 | A/B/C/D | 2-176 | [»] | |
1G4B | X-ray | 7.00 | M/N/O/P | 2-176 | [»] | |
1HQY | X-ray | 2.80 | A/B/C/D | 2-176 | [»] | |
1HT1 | X-ray | 2.80 | A/B/C/D/V/X/Y/Z | 2-176 | [»] | |
1HT2 | X-ray | 2.80 | A/B/C/D/I/J/K/L | 2-176 | [»] | |
1NED | X-ray | 3.80 | A/B/C | 2-176 | [»] | |
4G4E | X-ray | 2.89 | A/B/C/D/E/F/G/H/I/J/K/L | 2-175 | [»] | |
5JI3 | X-ray | 3.00 | A/B/C/D | 1-176 | [»] | |
6PXI | X-ray | 3.45 | A/B/C/D | 2-175 | [»] | |
SMRi | P0A7B8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261781, 287 interactors |
ComplexPortali | CPX-2104, HslUV protease complex |
DIPi | DIP-35866N |
IntActi | P0A7B8, 33 interactors |
STRINGi | 511145.b3932 |
Protein family/group databases
MEROPSi | T01.006 |
2D gel databases
SWISS-2DPAGEi | P0A7B8 |
Proteomic databases
jPOSTi | P0A7B8 |
PaxDbi | P0A7B8 |
PRIDEi | P0A7B8 |
Genome annotation databases
EnsemblBacteriai | AAC76914; AAC76914; b3932 BAE77378; BAE77378; BAE77378 |
GeneIDi | 67417555 948429 |
KEGGi | ecj:JW3903 eco:b3932 |
PATRICi | fig|1411691.4.peg.2773 |
Organism-specific databases
EchoBASEi | EB1627 |
Phylogenomic databases
eggNOGi | COG5405, Bacteria |
HOGENOMi | CLU_093872_1_0_6 |
InParanoidi | P0A7B8 |
PhylomeDBi | P0A7B8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11676-MONOMER |
BRENDAi | 3.4.25.2, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A7B8 |
PROi | PR:P0A7B8 |
Family and domain databases
CDDi | cd01913, protease_HslV, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
HAMAPi | MF_00248, HslV, 1 hit |
InterProi | View protein in InterPro IPR022281, ATP-dep_Prtase_HsIV_su IPR029055, Ntn_hydrolases_N IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR32194, PTHR32194, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PIRSFi | PIRSF039093, HslV, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR03692, ATP_dep_HslV, 1 hit |
PROSITEi | View protein in PROSITE PS51476, PROTEASOME_BETA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HSLV_ECOLI | |
Accessioni | P0A7B8Primary (citable) accession number: P0A7B8 Secondary accession number(s): P31059, P97542, Q2M8M8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families