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UniProtKB - P0A7B3 (NADK_ECOLI)
Protein
NAD kinase
Gene
nadK
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates (UTP, CTP, GTP, dATP, TTP) as phosphoryl donors, while nucleoside mono- or diphosphates and poly(P) cannot.
UniRule annotation3 PublicationsCatalytic activityi
- EC:2.7.1.23UniRule annotation3 Publications
Cofactori
a divalent metal cationUniRule annotation1 Publication
Activity regulationi
Competitively and allosterically inhibited by NADH and NADPH at physiological concentrations, whereas inhibition by NADP is only slight. Inhibited by p-chloromercuribenzoate and HgCl2.2 Publications
Kineticsi
kcat is 55 sec(-1) for kinase activity with ATP. kcat is 125 sec(-1) for kinase activity with NAD.
- KM=2 mM for NAD (at pH 7 and 37 degrees Celsius)3 Publications
- KM=2.5 mM for ATP (at pH 7 and 37 degrees Celsius)3 Publications
- KM=4.1 mM for magnesium (at pH 7 and 37 degrees Celsius)3 Publications
- Vmax=12.86 µmol/min/mg enzyme (at pH 7 and 37 degrees Celsius)3 Publications
pH dependencei
Optimum pH is between 7.2 and 7.5.3 Publications
Temperature dependencei
Optimum temperature is 60 degrees Celsius and half of the activity is lost on treatment at 65 degrees Celsius for 10 minutes.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 158 | NADUniRule annotation | 1 | |
Binding sitei | 175 | NADUniRule annotation | 1 | |
Sitei | 175 | Responsible for conferring strict specificity to NAD | 1 | |
Binding sitei | 177 | NADUniRule annotation | 1 | |
Binding sitei | 185 | NAD; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 247 | NADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 73 – 74 | NADUniRule annotation | 2 | |
Nucleotide bindingi | 147 – 148 | NADUniRule annotation | 2 | |
Nucleotide bindingi | 188 – 193 | NADUniRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- metal ion binding Source: UniProtKB-UniRule
- NAD+ kinase activity Source: EcoCyc
- NAD binding Source: UniProtKB
GO - Biological processi
- NAD metabolic process Source: InterPro
- NADP biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-541 |
BRENDAi | 2.7.1.23, 2026 |
SABIO-RKi | P0A7B3 |
Names & Taxonomyi
Protein namesi | Recommended name: NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)Alternative name(s): ATP-dependent NAD kinaseUniRule annotation |
Gene namesi | Name:nadKUniRule annotation Synonyms:yfjB, yfjE Ordered Locus Names:b2615, JW2596 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 175 | R → E: Does not exhibit NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → G: Exhibits NADH kinase activity in addition to NAD kinase activity. Reduces the Vmax of the NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → H: Exhibits NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → I: Does not exhibit NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → K: Does not exhibit NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → Q: Exhibits NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | R → T: Exhibits NADH kinase activity in addition to NAD kinase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120616 | 1 – 292 | NAD kinaseAdd BLAST | 292 |
Proteomic databases
jPOSTi | P0A7B3 |
PaxDbi | P0A7B3 |
PRIDEi | P0A7B3 |
Interactioni
Subunit structurei
Homohexamer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260607, 19 interactors 851428, 4 interactors |
DIPi | DIP-48103N |
IntActi | P0A7B3, 4 interactors |
STRINGi | 511145.b2615 |
Family & Domainsi
Sequence similaritiesi
Belongs to the NAD kinase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0061, Bacteria |
HOGENOMi | CLU_008831_0_1_6 |
InParanoidi | P0A7B3 |
OMAi | VNLGHVG |
PhylomeDBi | P0A7B3 |
Family and domain databases
Gene3Di | 2.60.200.30, 1 hit 3.40.50.10330, 1 hit |
HAMAPi | MF_00361, NAD_kinase, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR017437, ATP-NAD_kinase_PpnK-typ_C IPR016064, NAD/diacylglycerol_kinase_sf IPR002504, NADK |
Pfami | View protein in Pfam PF01513, NAD_kinase, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A7B3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK
60 70 80 90 100
NVKTGTLAEI GQLADLAVVV GGDGNMLGAA RTLARYDIKV IGINRGNLGF
110 120 130 140 150
LTDLDPDNAQ QQLADVLEGH YISEKRFLLE AQVCQQDCQK RISTAINEVV
160 170 180 190 200
LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI ISTPTGSTAY SLSAGGPILT
210 220 230 240 250
PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL EISCDSQIAL
260 270 280 290
PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF
Sequence cautioni
The sequence X07863 differs from that shown. Reason: Frameshift.Curated
The sequence Y00357 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36840 Genomic DNA Translation: AAA79785.1 U00096 Genomic DNA Translation: AAC75664.1 AP009048 Genomic DNA Translation: BAA16500.1 X07863 Genomic DNA No translation available. Y00357 Genomic DNA No translation available. |
PIRi | B65040 |
RefSeqi | NP_417105.1, NC_000913.3 WP_001059169.1, NZ_STEB01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC75664; AAC75664; b2615 BAA16500; BAA16500; BAA16500 |
GeneIDi | 67414068 947092 |
KEGGi | ecj:JW2596 eco:b2615 |
PATRICi | fig|1411691.4.peg.4124 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36840 Genomic DNA Translation: AAA79785.1 U00096 Genomic DNA Translation: AAC75664.1 AP009048 Genomic DNA Translation: BAA16500.1 X07863 Genomic DNA No translation available. Y00357 Genomic DNA No translation available. |
PIRi | B65040 |
RefSeqi | NP_417105.1, NC_000913.3 WP_001059169.1, NZ_STEB01000040.1 |
3D structure databases
AlphaFoldDBi | P0A7B3 |
SMRi | P0A7B3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260607, 19 interactors 851428, 4 interactors |
DIPi | DIP-48103N |
IntActi | P0A7B3, 4 interactors |
STRINGi | 511145.b2615 |
Proteomic databases
jPOSTi | P0A7B3 |
PaxDbi | P0A7B3 |
PRIDEi | P0A7B3 |
Genome annotation databases
EnsemblBacteriai | AAC75664; AAC75664; b2615 BAA16500; BAA16500; BAA16500 |
GeneIDi | 67414068 947092 |
KEGGi | ecj:JW2596 eco:b2615 |
PATRICi | fig|1411691.4.peg.4124 |
Organism-specific databases
EchoBASEi | EB2109 |
Phylogenomic databases
eggNOGi | COG0061, Bacteria |
HOGENOMi | CLU_008831_0_1_6 |
InParanoidi | P0A7B3 |
OMAi | VNLGHVG |
PhylomeDBi | P0A7B3 |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-541 |
BRENDAi | 2.7.1.23, 2026 |
SABIO-RKi | P0A7B3 |
Miscellaneous databases
PROi | PR:P0A7B3 |
Family and domain databases
Gene3Di | 2.60.200.30, 1 hit 3.40.50.10330, 1 hit |
HAMAPi | MF_00361, NAD_kinase, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR017437, ATP-NAD_kinase_PpnK-typ_C IPR016064, NAD/diacylglycerol_kinase_sf IPR002504, NADK |
Pfami | View protein in Pfam PF01513, NAD_kinase, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NADK_ECOLI | |
Accessioni | P0A7B3Primary (citable) accession number: P0A7B3 Secondary accession number(s): P37768, P46140, P77490 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families