UniProtKB - P0A7A9 (IPYR_ECOLI)
Protein
Inorganic pyrophosphatase
Gene
ppa
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.UniRule annotation
Catalytic activityi
- EC:3.6.1.1UniRule annotation
Cofactori
Mg2+UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | SubstrateUniRule annotation | 1 | |
Binding sitei | 44 | SubstrateUniRule annotation | 1 | |
Binding sitei | 56 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 66 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 71 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 71 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 103 | Magnesium 1UniRule annotation | 1 | |
Binding sitei | 142 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- inorganic diphosphatase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
- triphosphatase activity Source: EcoCyc
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- phosphate-containing compound metabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:INORGPYROPHOSPHAT-MONOMER MetaCyc:INORGPYROPHOSPHAT-MONOMER |
BRENDAi | 3.6.1.1, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)Alternative name(s): Pyrophosphate phospho-hydrolaseUniRule annotation Short name: PPaseUniRule annotation |
Gene namesi | Name:ppaUniRule annotation Ordered Locus Names:b4226, JW4185 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | E → D: 16% activity. | 1 | |
Mutagenesisi | 30 | K → R: 2% activity. | 1 | |
Mutagenesisi | 32 | E → D: 6% activity. | 1 | |
Mutagenesisi | 44 | R → K: 10% activity. | 1 | |
Mutagenesisi | 52 | Y → F: 64% activity. | 1 | |
Mutagenesisi | 56 | Y → F: 7% activity. | 1 | |
Mutagenesisi | 66 | D → E: 6% activity. | 1 | |
Mutagenesisi | 68 | D → E: 1% activity. | 1 | |
Mutagenesisi | 71 | D → E: No activity. | 1 | |
Mutagenesisi | 98 | D → E: 22% activity. | 1 | |
Mutagenesisi | 98 | D → V: No activity. | 1 | |
Mutagenesisi | 99 | E → V: 33% activity. | 1 | |
Mutagenesisi | 103 | D → E: 3% activity. | 1 | |
Mutagenesisi | 103 | D → V: No activity. | 1 | |
Mutagenesisi | 105 | K → I: No activity. | 1 | |
Mutagenesisi | 105 | K → R: 3% activity. | 1 | |
Mutagenesisi | 142 | Y → F: 22% activity. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000137495 | 2 – 176 | Inorganic pyrophosphataseAdd BLAST | 175 |
Proteomic databases
jPOSTi | P0A7A9 |
PaxDbi | P0A7A9 |
PRIDEi | P0A7A9 |
2D gel databases
SWISS-2DPAGEi | P0A7A9 |
Interactioni
Subunit structurei
Homohexamer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4259312, 48 interactors |
DIPi | DIP-36217N |
IntActi | P0A7A9, 37 interactors |
STRINGi | 511145.b4226 |
Chemistry databases
BindingDBi | P0A7A9 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P0A7A9 |
SMRi | P0A7A9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A7A9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PPase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0221, Bacteria |
HOGENOMi | CLU_073198_1_0_6 |
InParanoidi | P0A7A9 |
PhylomeDBi | P0A7A9 |
Family and domain databases
CDDi | cd00412, pyrophosphatase, 1 hit |
Gene3Di | 3.90.80.10, 1 hit |
HAMAPi | MF_00209, Inorganic_PPase, 1 hit |
InterProi | View protein in InterPro IPR008162, Pyrophosphatase IPR036649, Pyrophosphatase_sf |
PANTHERi | PTHR10286, PTHR10286, 1 hit |
Pfami | View protein in Pfam PF00719, Pyrophosphatase, 1 hit |
SUPFAMi | SSF50324, SSF50324, 1 hit |
PROSITEi | View protein in PROSITE PS00387, PPASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A7A9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM
60 70 80 90 100
FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA
110 120 130 140 150
GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW
160 170
VKVEGWENAE AAKAEIVASF ERAKNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23550 Unassigned DNA Translation: AAB88583.1 U14003 Genomic DNA Translation: AAA97123.1 U00096 Genomic DNA Translation: AAC77183.1 AP009048 Genomic DNA Translation: BAE78227.1 |
PIRi | A27648, PWEC |
RefSeqi | NP_418647.1, NC_000913.3 WP_000055075.1, NZ_SSUV01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77183; AAC77183; b4226 BAE78227; BAE78227; BAE78227 |
GeneIDi | 948748 |
KEGGi | ecj:JW4185 eco:b4226 |
PATRICi | fig|1411691.4.peg.2475 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23550 Unassigned DNA Translation: AAB88583.1 U14003 Genomic DNA Translation: AAA97123.1 U00096 Genomic DNA Translation: AAC77183.1 AP009048 Genomic DNA Translation: BAE78227.1 |
PIRi | A27648, PWEC |
RefSeqi | NP_418647.1, NC_000913.3 WP_000055075.1, NZ_SSUV01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FAJ | X-ray | 2.15 | A | 2-176 | [»] | |
1I40 | X-ray | 1.10 | A | 2-176 | [»] | |
1I6T | X-ray | 1.20 | A | 2-176 | [»] | |
1IGP | X-ray | 2.20 | A | 2-176 | [»] | |
1INO | X-ray | 2.20 | A | 2-176 | [»] | |
1IPW | X-ray | 2.30 | A/B | 2-176 | [»] | |
1JFD | X-ray | 2.20 | A/B | 2-176 | [»] | |
1MJW | X-ray | 1.95 | A/B | 2-176 | [»] | |
1MJX | X-ray | 2.15 | A/B | 2-176 | [»] | |
1MJY | X-ray | 2.10 | A/B | 2-176 | [»] | |
1MJZ | X-ray | 2.20 | A | 2-176 | [»] | |
1OBW | X-ray | 1.90 | A/B/C | 2-176 | [»] | |
2AU6 | X-ray | 1.20 | A | 2-176 | [»] | |
2AU7 | X-ray | 1.05 | A | 2-176 | [»] | |
2AU8 | X-ray | 1.65 | A | 2-176 | [»] | |
2AU9 | X-ray | 1.30 | A | 2-176 | [»] | |
2AUU | X-ray | 1.22 | A | 2-176 | [»] | |
2EIP | X-ray | 2.20 | A/B | 2-176 | [»] | |
4UM4 | X-ray | 2.65 | A/B/C | 1-176 | [»] | |
SASBDBi | P0A7A9 | |||||
SMRi | P0A7A9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259312, 48 interactors |
DIPi | DIP-36217N |
IntActi | P0A7A9, 37 interactors |
STRINGi | 511145.b4226 |
Chemistry databases
BindingDBi | P0A7A9 |
2D gel databases
SWISS-2DPAGEi | P0A7A9 |
Proteomic databases
jPOSTi | P0A7A9 |
PaxDbi | P0A7A9 |
PRIDEi | P0A7A9 |
Genome annotation databases
EnsemblBacteriai | AAC77183; AAC77183; b4226 BAE78227; BAE78227; BAE78227 |
GeneIDi | 948748 |
KEGGi | ecj:JW4185 eco:b4226 |
PATRICi | fig|1411691.4.peg.2475 |
Organism-specific databases
EchoBASEi | EB0748 |
Phylogenomic databases
eggNOGi | COG0221, Bacteria |
HOGENOMi | CLU_073198_1_0_6 |
InParanoidi | P0A7A9 |
PhylomeDBi | P0A7A9 |
Enzyme and pathway databases
BioCyci | EcoCyc:INORGPYROPHOSPHAT-MONOMER MetaCyc:INORGPYROPHOSPHAT-MONOMER |
BRENDAi | 3.6.1.1, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A7A9 |
PROi | PR:P0A7A9 |
Family and domain databases
CDDi | cd00412, pyrophosphatase, 1 hit |
Gene3Di | 3.90.80.10, 1 hit |
HAMAPi | MF_00209, Inorganic_PPase, 1 hit |
InterProi | View protein in InterPro IPR008162, Pyrophosphatase IPR036649, Pyrophosphatase_sf |
PANTHERi | PTHR10286, PTHR10286, 1 hit |
Pfami | View protein in Pfam PF00719, Pyrophosphatase, 1 hit |
SUPFAMi | SSF50324, SSF50324, 1 hit |
PROSITEi | View protein in PROSITE PS00387, PPASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IPYR_ECOLI | |
Accessioni | P0A7A9Primary (citable) accession number: P0A7A9 Secondary accession number(s): P17288, Q2M679 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 133 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families