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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei30SubstrateUniRule annotation1
Binding sitei44SubstrateUniRule annotation1
Binding sitei56SubstrateUniRule annotation1
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi66Magnesium 1UniRule annotation1
Metal bindingi71Magnesium 1UniRule annotation1
Metal bindingi71Magnesium 2UniRule annotation1
Metal bindingi103Magnesium 1UniRule annotation1
Binding sitei142SubstrateUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • phosphate-containing compound metabolic process Source: GO_Central

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:INORGPYROPHOSPHAT-MONOMER
MetaCyc:INORGPYROPHOSPHAT-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
3.6.1.1 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Pyrophosphate phospho-hydrolaseUniRule annotation
Short name:
PPaseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ppaUniRule annotation
Ordered Locus Names:b4226, JW4185
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG10755 ppa

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi21E → D: 16% activity. 1
Mutagenesisi30K → R: 2% activity. 1
Mutagenesisi32E → D: 6% activity. 1
Mutagenesisi44R → K: 10% activity. 1
Mutagenesisi52Y → F: 64% activity. 1
Mutagenesisi56Y → F: 7% activity. 1
Mutagenesisi66D → E: 6% activity. 1
Mutagenesisi68D → E: 1% activity. 1
Mutagenesisi71D → E: No activity. 1
Mutagenesisi98D → E: 22% activity. 1
Mutagenesisi98D → V: No activity. 1
Mutagenesisi99E → V: 33% activity. 1
Mutagenesisi103D → E: 3% activity. 1
Mutagenesisi103D → V: No activity. 1
Mutagenesisi105K → I: No activity. 1
Mutagenesisi105K → R: 3% activity. 1
Mutagenesisi142Y → F: 22% activity. 1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001374952 – 176Inorganic pyrophosphataseAdd BLAST175

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
P0A7A9

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0A7A9

PRoteomics IDEntifications database

More...
PRIDEi
P0A7A9

2D gel databases

Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

More...
SWISS-2DPAGEi
P0A7A9

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4259312, 48 interactors

Database of interacting proteins

More...
DIPi
DIP-36217N

Protein interaction database and analysis system

More...
IntActi
P0A7A9, 37 interactors

STRING: functional protein association networks

More...
STRINGi
316385.ECDH10B_4421

Chemistry databases

BindingDB database of measured binding affinities

More...
BindingDBi
P0A7A9

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P0A7A9

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0A7A9

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P0A7A9

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PPase family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105F0N Bacteria
COG0221 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000236473

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0A7A9

KEGG Orthology (KO)

More...
KOi
K01507

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0A7A9

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00412 pyrophosphatase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.90.80.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00209 Inorganic_PPase, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008162 Pyrophosphatase
IPR036649 Pyrophosphatase_sf

The PANTHER Classification System

More...
PANTHERi
PTHR10286 PTHR10286, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00719 Pyrophosphatase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50324 SSF50324, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00387 PPASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7A9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM
60 70 80 90 100
FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA
110 120 130 140 150
GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW
160 170
VKVEGWENAE AAKAEIVASF ERAKNK
Length:176
Mass (Da):19,704
Last modified:January 23, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4C97E51F3BE650DE
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M23550 Unassigned DNA Translation: AAB88583.1
U14003 Genomic DNA Translation: AAA97123.1
U00096 Genomic DNA Translation: AAC77183.1
AP009048 Genomic DNA Translation: BAE78227.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A27648 PWEC

NCBI Reference Sequences

More...
RefSeqi
NP_418647.1, NC_000913.3
WP_000055075.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC77183; AAC77183; b4226
BAE78227; BAE78227; BAE78227

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948748

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW4185
eco:b4226

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2475

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23550 Unassigned DNA Translation: AAB88583.1
U14003 Genomic DNA Translation: AAA97123.1
U00096 Genomic DNA Translation: AAC77183.1
AP009048 Genomic DNA Translation: BAE78227.1
PIRiA27648 PWEC
RefSeqiNP_418647.1, NC_000913.3
WP_000055075.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAJX-ray2.15A2-176[»]
1I40X-ray1.10A2-176[»]
1I6TX-ray1.20A2-176[»]
1IGPX-ray2.20A2-176[»]
1INOX-ray2.20A2-176[»]
1IPWX-ray2.30A/B2-176[»]
1JFDX-ray2.20A/B2-176[»]
1MJWX-ray1.95A/B2-176[»]
1MJXX-ray2.15A/B2-176[»]
1MJYX-ray2.10A/B2-176[»]
1MJZX-ray2.20A2-176[»]
1OBWX-ray1.90A/B/C2-176[»]
2AU6X-ray1.20A2-176[»]
2AU7X-ray1.05A2-176[»]
2AU8X-ray1.65A2-176[»]
2AU9X-ray1.30A2-176[»]
2AUUX-ray1.22A2-176[»]
2EIPX-ray2.20A/B2-176[»]
4UM4X-ray2.65A/B/C1-176[»]
ProteinModelPortaliP0A7A9
SMRiP0A7A9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259312, 48 interactors
DIPiDIP-36217N
IntActiP0A7A9, 37 interactors
STRINGi316385.ECDH10B_4421

Chemistry databases

BindingDBiP0A7A9

2D gel databases

SWISS-2DPAGEiP0A7A9

Proteomic databases

EPDiP0A7A9
PaxDbiP0A7A9
PRIDEiP0A7A9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77183; AAC77183; b4226
BAE78227; BAE78227; BAE78227
GeneIDi948748
KEGGiecj:JW4185
eco:b4226
PATRICifig|1411691.4.peg.2475

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0748
EcoGeneiEG10755 ppa

Phylogenomic databases

eggNOGiENOG4105F0N Bacteria
COG0221 LUCA
HOGENOMiHOG000236473
InParanoidiP0A7A9
KOiK01507
PhylomeDBiP0A7A9

Enzyme and pathway databases

BioCyciEcoCyc:INORGPYROPHOSPHAT-MONOMER
MetaCyc:INORGPYROPHOSPHAT-MONOMER
BRENDAi3.6.1.1 2026

Miscellaneous databases

EvolutionaryTraceiP0A7A9

Protein Ontology

More...
PROi
PR:P0A7A9

Family and domain databases

CDDicd00412 pyrophosphatase, 1 hit
Gene3Di3.90.80.10, 1 hit
HAMAPiMF_00209 Inorganic_PPase, 1 hit
InterProiView protein in InterPro
IPR008162 Pyrophosphatase
IPR036649 Pyrophosphatase_sf
PANTHERiPTHR10286 PTHR10286, 1 hit
PfamiView protein in Pfam
PF00719 Pyrophosphatase, 1 hit
SUPFAMiSSF50324 SSF50324, 1 hit
PROSITEiView protein in PROSITE
PS00387 PPASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiIPYR_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A7A9
Secondary accession number(s): P17288, Q2M679
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: December 5, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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