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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase A (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), Probable phosphoglycerate mutase GpmB (gpmB)
  4. Enolase (eno)
  5. Pyruvate kinase I (pykF), Pyruvate kinase II (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei146SubstrateBy similarity1
Binding sitei197ATPBy similarity1
Binding sitei314ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi340 – 343ATPBy similarity4

GO - Molecular functioni

  • ADP binding Source: GO_Central
  • ATP binding Source: GO_Central
  • phosphoglycerate kinase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGK
MetaCyc:PGK
BRENDAi2.7.2.3 2026
SABIO-RKiP0A799
UniPathwayi
UPA00109;UER00185

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:b2926, JW2893
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10703 pgk

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001459412 – 387Phosphoglycerate kinaseAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A799
PaxDbiP0A799
PRIDEiP0A799

2D gel databases

SWISS-2DPAGEiP0A799

PTM databases

iPTMnetiP0A799

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262834, 42 interactors
DIPiDIP-36163N
IntActiP0A799, 16 interactors
STRINGi316385.ECDH10B_3101

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A799
SMRiP0A799
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A799

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingBy similarity3
Regioni59 – 62Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZA Bacteria
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiP0A799
KOiK00927
PhylomeDBiP0A799

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 2 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A799-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVIKMTDLD LAGKRVFIRA DLNVPVKDGK VTSDARIRAS LPTIELALKQ
60 70 80 90 100
GAKVMVTSHL GRPTEGEYNE EFSLLPVVNY LKDKLSNPVR LVKDYLDGVD
110 120 130 140 150
VAEGELVVLE NVRFNKGEKK DDETLSKKYA ALCDVFVMDA FGTAHRAQAS
160 170 180 190 200
THGIGKFADV ACAGPLLAAE LDALGKALKE PARPMVAIVG GSKVSTKLTV
210 220 230 240 250
LDSLSKIADQ LIVGGGIANT FIAAQGHDVG KSLYEADLVD EAKRLLTTCN
260 270 280 290 300
IPVPSDVRVA TEFSETAPAT LKSVNDVKAD EQILDIGDAS AQELAEILKN
310 320 330 340 350
AKTILWNGPV GVFEFPNFRK GTEIVANAIA DSEAFSIAGG GDTLAAIDLF
360 370 380
GIADKISYIS TGGGAFLEFV EGKVLPAVAM LEERAKK
Length:387
Mass (Da):41,118
Last modified:January 23, 2007 - v2
Checksum:iFAD7D66D100514B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA Translation: CAA32604.1
U28377 Genomic DNA Translation: AAA69093.1
U00096 Genomic DNA Translation: AAC75963.1
AP009048 Genomic DNA Translation: BAE76990.1
PIRiS04733 TVECG
RefSeqiNP_417401.1, NC_000913.3
WP_000111269.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75963; AAC75963; b2926
BAE76990; BAE76990; BAE76990
GeneIDi947414
KEGGiecj:JW2893
eco:b2926
PATRICifig|1411691.4.peg.3806

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA Translation: CAA32604.1
U28377 Genomic DNA Translation: AAA69093.1
U00096 Genomic DNA Translation: AAC75963.1
AP009048 Genomic DNA Translation: BAE76990.1
PIRiS04733 TVECG
RefSeqiNP_417401.1, NC_000913.3
WP_000111269.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZMRX-ray2.40A1-387[»]
ProteinModelPortaliP0A799
SMRiP0A799
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262834, 42 interactors
DIPiDIP-36163N
IntActiP0A799, 16 interactors
STRINGi316385.ECDH10B_3101

PTM databases

iPTMnetiP0A799

2D gel databases

SWISS-2DPAGEiP0A799

Proteomic databases

EPDiP0A799
PaxDbiP0A799
PRIDEiP0A799

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75963; AAC75963; b2926
BAE76990; BAE76990; BAE76990
GeneIDi947414
KEGGiecj:JW2893
eco:b2926
PATRICifig|1411691.4.peg.3806

Organism-specific databases

EchoBASEiEB0697
EcoGeneiEG10703 pgk

Phylogenomic databases

eggNOGiENOG4105BZA Bacteria
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiP0A799
KOiK00927
PhylomeDBiP0A799

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00185

BioCyciEcoCyc:PGK
MetaCyc:PGK
BRENDAi2.7.2.3 2026
SABIO-RKiP0A799

Miscellaneous databases

EvolutionaryTraceiP0A799
PROiPR:P0A799

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 2 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_ECOLI
AccessioniPrimary (citable) accession number: P0A799
Secondary accession number(s): P11665, Q2M9R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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