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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase A (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. Probable phosphoglycerate mutase GpmB (gpmB), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase I (pykF), Pyruvate kinase II (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei146SubstrateBy similarity1
Binding sitei197ATPBy similarity1
Binding sitei314ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi340 – 343ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoglycerate kinase activity Source: EcoCyc

GO - Biological processi

  • glycolytic process Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGK
MetaCyc:PGK
BRENDAi2.7.2.3 2026
SABIO-RKiP0A799
UniPathwayiUPA00109; UER00185

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:b2926, JW2893
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10703 pgk

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001459412 – 387Phosphoglycerate kinaseAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A799
PaxDbiP0A799
PRIDEiP0A799

2D gel databases

SWISS-2DPAGEiP0A799

PTM databases

iPTMnetiP0A799

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262834, 42 interactors
DIPiDIP-36163N
IntActiP0A799, 16 interactors
STRINGi316385.ECDH10B_3101

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi15 – 19Combined sources5
Beta strandi30 – 32Combined sources3
Helixi35 – 49Combined sources15
Beta strandi53 – 57Combined sources5
Helixi70 – 72Combined sources3
Helixi75 – 84Combined sources10
Beta strandi89 – 93Combined sources5
Beta strandi106 – 109Combined sources4
Helixi112 – 114Combined sources3
Turni116 – 121Combined sources6
Helixi123 – 131Combined sources9
Beta strandi134 – 138Combined sources5
Helixi141 – 143Combined sources3
Turni149 – 153Combined sources5
Helixi154 – 157Combined sources4
Beta strandi158 – 163Combined sources6
Helixi165 – 178Combined sources14
Beta strandi182 – 192Combined sources11
Turni194 – 197Combined sources4
Helixi198 – 205Combined sources8
Beta strandi209 – 215Combined sources7
Helixi216 – 224Combined sources9
Helixi236 – 238Combined sources3
Helixi239 – 246Combined sources8
Beta strandi255 – 266Combined sources12
Beta strandi270 – 273Combined sources4
Helixi274 – 276Combined sources3
Beta strandi282 – 286Combined sources5
Helixi288 – 300Combined sources13
Beta strandi302 – 308Combined sources7
Helixi316 – 318Combined sources3
Helixi320 – 331Combined sources12
Beta strandi332 – 338Combined sources7
Helixi341 – 350Combined sources10
Helixi353 – 355Combined sources3
Beta strandi356 – 359Combined sources4
Helixi364 – 370Combined sources7
Helixi376 – 384Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZMRX-ray2.40A1-387[»]
ProteinModelPortaliP0A799
SMRiP0A799
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A799

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingBy similarity3
Regioni59 – 62Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZA Bacteria
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiP0A799
KOiK00927
OMAiDMIFDIG
PhylomeDBiP0A799

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 3 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIKMTDLD LAGKRVFIRA DLNVPVKDGK VTSDARIRAS LPTIELALKQ
60 70 80 90 100
GAKVMVTSHL GRPTEGEYNE EFSLLPVVNY LKDKLSNPVR LVKDYLDGVD
110 120 130 140 150
VAEGELVVLE NVRFNKGEKK DDETLSKKYA ALCDVFVMDA FGTAHRAQAS
160 170 180 190 200
THGIGKFADV ACAGPLLAAE LDALGKALKE PARPMVAIVG GSKVSTKLTV
210 220 230 240 250
LDSLSKIADQ LIVGGGIANT FIAAQGHDVG KSLYEADLVD EAKRLLTTCN
260 270 280 290 300
IPVPSDVRVA TEFSETAPAT LKSVNDVKAD EQILDIGDAS AQELAEILKN
310 320 330 340 350
AKTILWNGPV GVFEFPNFRK GTEIVANAIA DSEAFSIAGG GDTLAAIDLF
360 370 380
GIADKISYIS TGGGAFLEFV EGKVLPAVAM LEERAKK
Length:387
Mass (Da):41,118
Last modified:January 23, 2007 - v2
Checksum:iFAD7D66D100514B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA Translation: CAA32604.1
U28377 Genomic DNA Translation: AAA69093.1
U00096 Genomic DNA Translation: AAC75963.1
AP009048 Genomic DNA Translation: BAE76990.1
PIRiS04733 TVECG
RefSeqiNP_417401.1, NC_000913.3
WP_000111269.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75963; AAC75963; b2926
BAE76990; BAE76990; BAE76990
GeneIDi947414
KEGGiecj:JW2893
eco:b2926
PATRICifig|1411691.4.peg.3806

Similar proteinsi

Entry informationi

Entry nameiPGK_ECOLI
AccessioniPrimary (citable) accession number: P0A799
Secondary accession number(s): P11665, Q2M9R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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