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UniProtKB - P0A796 (PFKA_ECOLI)
Protein
ATP-dependent 6-phosphofructokinase isozyme 1
Gene
pfkA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
UniRule annotationCatalytic activityi
- EC:2.7.1.11UniRule annotation
Cofactori
Mg2+UniRule annotation1 Publication
Activity regulationi
Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | ATP; via amide nitrogenUniRule annotation1 Publication | 1 | |
Metal bindingi | 104 | Magnesium; catalyticUniRule annotation1 Publication | 1 | |
Active sitei | 128 | Proton acceptorUniRule annotation2 Publications | 1 | |
Binding sitei | 155 | ADP; allosteric activatorUniRule annotation1 Publication | 1 | |
Binding sitei | 163 | Substrate; shared with dimeric partnerUniRule annotation1 Publication | 1 | |
Binding sitei | 212 | ADP; allosteric activatorUniRule annotationBy similarity | 1 | |
Binding sitei | 223 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 244 | Substrate; shared with dimeric partnerUniRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 73 – 74 | ATPUniRule annotation1 Publication | 2 | |
Nucleotide bindingi | 103 – 106 | ATPUniRule annotation1 Publication | 4 |
GO - Molecular functioni
- 6-phosphofructokinase activity Source: EcoCyc
- AMP binding Source: GO_Central
- ATP binding Source: EcoliWiki
- fructose-6-phosphate binding Source: GO_Central
- GDP binding Source: EcoCyc
- identical protein binding Source: EcoliWiki
- magnesium ion binding Source: EcoliWiki
- monosaccharide binding Source: GO_Central
- ribonucleotide binding Source: EcoliWiki
GO - Biological processi
- canonical glycolysis Source: GO_Central
- cellular carbohydrate catabolic process Source: EcoliWiki
- fructose 1,6-bisphosphate metabolic process Source: GO_Central
- fructose 6-phosphate metabolic process Source: GO_Central
- glucose catabolic process Source: EcoliWiki
- glycolytic process Source: EcoliWiki
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Biological process | Glycolysis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:6PFK-1-MONOMER |
BRENDAi | 2.7.1.11, 2026 |
SABIO-RKi | P0A796 |
UniPathwayi | UPA00109;UER00182 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent 6-phosphofructokinase isozyme 1UniRule annotation (EC:2.7.1.11UniRule annotation)Short name: ATP-PFK 1UniRule annotation Short name: Phosphofructokinase 1UniRule annotation Alternative name(s): 6-phosphofructokinase isozyme I Phosphohexokinase 1UniRule annotation |
Gene namesi | Name:pfkAUniRule annotation Ordered Locus Names:b3916, JW3887 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- 6-phosphofructokinase complex Source: EcoliWiki
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 128 | D → S: 18000-fold reduction of catalytic rate. 1 Publication | 1 | |
Mutagenesisi | 172 | R → S: 3.4-fold reduction in turnover numbers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000111950 | 1 – 320 | ATP-dependent 6-phosphofructokinase isozyme 1Add BLAST | 320 |
Proteomic databases
jPOSTi | P0A796 |
PaxDbi | P0A796 |
PRIDEi | P0A796 |
2D gel databases
SWISS-2DPAGEi | P0A796 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation1 PublicationBinary interactionsi
P0A796
With | #Exp. | IntAct |
---|---|---|
moeA [P12281] | 2 | EBI-554405,EBI-554393 |
prfC [P0A7I4] | 2 | EBI-554405,EBI-556252 |
ucpA [P37440] | 2 | EBI-554405,EBI-559387 |
GO - Molecular functioni
- identical protein binding Source: EcoliWiki
Protein-protein interaction databases
BioGRIDi | 4261224, 17 interactors |
DIPi | DIP-35841N |
IntActi | P0A796, 7 interactors |
STRINGi | 511145.b3916 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A796 |
SMRi | P0A796 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A796 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 26 | ADP binding; allosteric activator; shared with dimeric partnerUniRule annotation1 Publication | 5 | |
Regioni | 55 – 60 | ADP binding; allosteric activator; shared with dimeric partnerUniRule annotation1 Publication | 6 | |
Regioni | 126 – 128 | Substrate bindingUniRule annotation1 Publication | 3 | |
Regioni | 170 – 172 | Substrate bindingUniRule annotationBy similarity | 3 | |
Regioni | 186 – 188 | ADP binding; allosteric activatorUniRule annotation1 Publication | 3 | |
Regioni | 214 – 216 | ADP binding; allosteric activatorUniRule annotation1 Publication | 3 | |
Regioni | 250 – 253 | Substrate bindingUniRule annotation1 Publication | 4 |
Sequence similaritiesi
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0205, Bacteria |
HOGENOMi | CLU_020655_0_1_6 |
InParanoidi | P0A796 |
OMAi | KFAVICV |
PhylomeDBi | P0A796 |
Family and domain databases
CDDi | cd00763, Bacterial_PFK, 1 hit |
Gene3Di | 3.40.50.460, 1 hit |
HAMAPi | MF_00339, Phosphofructokinase_I_B1, 1 hit |
InterProi | View protein in InterPro IPR022953, ATP_PFK IPR012003, ATP_PFK_prok-type IPR012828, PFKA_ATP_prok IPR015912, Phosphofructokinase_CS IPR000023, Phosphofructokinase_dom IPR035966, PKF_sf |
Pfami | View protein in Pfam PF00365, PFK, 1 hit |
PIRSFi | PIRSF000532, ATP_PFK_prok, 1 hit |
PRINTSi | PR00476, PHFRCTKINASE |
SUPFAMi | SSF53784, SSF53784, 1 hit |
TIGRFAMsi | TIGR02482, PFKA_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS00433, PHOSPHOFRUCTOKINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A796-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKKIGVLTS GGDAPGMNAA IRGVVRSALT EGLEVMGIYD GYLGLYEDRM
60 70 80 90 100
VQLDRYSVSD MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV
110 120 130 140 150
IGGDGSYMGA MRLTEMGFPC IGLPGTIDND IKGTDYTIGF FTALSTVVEA
160 170 180 190 200
IDRLRDTSSS HQRISVVEVM GRYCGDLTLA AAIAGGCEFV VVPEVEFSRE
210 220 230 240 250
DLVNEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG RETRATVLGH
260 270 280 290 300
IQRGGSPVPY DRILASRMGA YAIDLLLAGY GGRCVGIQNE QLVHHDIIDA
310 320
IENMKRPFKG DWLDCAKKLY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | F → C in CAA26356 (PubMed:3158524).Curated | 1 | |
Sequence conflicti | 103 – 104 | GD → DG in CAA26356 (PubMed:3158524).Curated | 2 | |
Sequence conflicti | 163 | R → P in CAA26356 (PubMed:3158524).Curated | 1 | |
Sequence conflicti | 317 – 319 | KKL → EKM in CAA26356 (PubMed:3158524).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02519 Genomic DNA Translation: CAA26356.1 L19201 Genomic DNA Translation: AAB03048.1 U00096 Genomic DNA Translation: AAC76898.1 AP009048 Genomic DNA Translation: BAE77394.1 |
PIRi | G65197, KIECFA |
RefSeqi | NP_418351.1, NC_000913.3 WP_000591795.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76898; AAC76898; b3916 BAE77394; BAE77394; BAE77394 |
GeneIDi | 66672176 948412 |
KEGGi | ecj:JW3887 eco:b3916 |
PATRICi | fig|1411691.4.peg.2789 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02519 Genomic DNA Translation: CAA26356.1 L19201 Genomic DNA Translation: AAB03048.1 U00096 Genomic DNA Translation: AAC76898.1 AP009048 Genomic DNA Translation: BAE77394.1 |
PIRi | G65197, KIECFA |
RefSeqi | NP_418351.1, NC_000913.3 WP_000591795.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PFK | X-ray | 2.40 | A/B | 1-320 | [»] | |
2PFK | X-ray | 2.40 | A/B/C/D | 1-320 | [»] | |
AlphaFoldDBi | P0A796 | |||||
SMRi | P0A796 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261224, 17 interactors |
DIPi | DIP-35841N |
IntActi | P0A796, 7 interactors |
STRINGi | 511145.b3916 |
2D gel databases
SWISS-2DPAGEi | P0A796 |
Proteomic databases
jPOSTi | P0A796 |
PaxDbi | P0A796 |
PRIDEi | P0A796 |
Genome annotation databases
EnsemblBacteriai | AAC76898; AAC76898; b3916 BAE77394; BAE77394; BAE77394 |
GeneIDi | 66672176 948412 |
KEGGi | ecj:JW3887 eco:b3916 |
PATRICi | fig|1411691.4.peg.2789 |
Organism-specific databases
EchoBASEi | EB0693 |
Phylogenomic databases
eggNOGi | COG0205, Bacteria |
HOGENOMi | CLU_020655_0_1_6 |
InParanoidi | P0A796 |
OMAi | KFAVICV |
PhylomeDBi | P0A796 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00182 |
BioCyci | EcoCyc:6PFK-1-MONOMER |
BRENDAi | 2.7.1.11, 2026 |
SABIO-RKi | P0A796 |
Miscellaneous databases
EvolutionaryTracei | P0A796 |
PROi | PR:P0A796 |
Family and domain databases
CDDi | cd00763, Bacterial_PFK, 1 hit |
Gene3Di | 3.40.50.460, 1 hit |
HAMAPi | MF_00339, Phosphofructokinase_I_B1, 1 hit |
InterProi | View protein in InterPro IPR022953, ATP_PFK IPR012003, ATP_PFK_prok-type IPR012828, PFKA_ATP_prok IPR015912, Phosphofructokinase_CS IPR000023, Phosphofructokinase_dom IPR035966, PKF_sf |
Pfami | View protein in Pfam PF00365, PFK, 1 hit |
PIRSFi | PIRSF000532, ATP_PFK_prok, 1 hit |
PRINTSi | PR00476, PHFRCTKINASE |
SUPFAMi | SSF53784, SSF53784, 1 hit |
TIGRFAMsi | TIGR02482, PFKA_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS00433, PHOSPHOFRUCTOKINASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PFKA_ECOLI | |
Accessioni | P0A796Primary (citable) accession number: P0A796 Secondary accession number(s): P06998, Q2M8L2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families