UniProtKB - P0A794 (PDXJ_ECOLI)
Protein
Pyridoxine 5'-phosphate synthase
Gene
pdxJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.1 Publication
Catalytic activityi
- 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H+ + 2 H2O + phosphate + pyridoxine 5'-phosphateEC:2.6.99.2
: pyridoxine 5'-phosphate biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- D-erythrose-4-phosphate dehydrogenase (epd), D-erythrose-4-phosphate dehydrogenase (epd)
- Erythronate-4-phosphate dehydrogenase (pdxB), Erythronate-4-phosphate dehydrogenase (pdxB)
- Phosphoserine aminotransferase (serC), Phosphoserine aminotransferase (serC)
- 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA), 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
- Pyridoxine 5'-phosphate synthase (pdxJ), Pyridoxine 5'-phosphate synthase (pdxJ)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 9 | 3-amino-2-oxopropyl phosphate | 1 | |
Binding sitei | 20 | 3-amino-2-oxopropyl phosphate | 1 | |
Active sitei | 45 | Proton acceptor | 1 | |
Binding sitei | 47 | 1-deoxy-D-xylulose 5-phosphate | 1 | |
Binding sitei | 52 | 1-deoxy-D-xylulose 5-phosphate | 1 | |
Active sitei | 72 | Proton acceptor | 1 | |
Binding sitei | 102 | 1-deoxy-D-xylulose 5-phosphate | 1 | |
Sitei | 153 | Transition state stabilizer | 1 | |
Active sitei | 193 | Proton donor | 1 | |
Binding sitei | 194 | 3-amino-2-oxopropyl phosphate; via amide nitrogen | 1 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- pyridoxine 5'-phosphate synthase activity Source: EcoCyc
GO - Biological processi
- pyridoxine biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Transferase |
Biological process | Pyridoxine biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:PDXJ-MONOMER MetaCyc:PDXJ-MONOMER |
BRENDAi | 2.6.99.2, 2026 |
UniPathwayi | UPA00244;UER00313 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdxJ Ordered Locus Names:b2564, JW2548 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Chemistry databases
DrugBanki | DB02496, 1-Deoxy-D-xylulose 5-phosphate DB02209, Pyridoxine phosphate DB02515, sn-glycerol 3-phosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000190114 | 2 – 243 | Pyridoxine 5'-phosphate synthaseAdd BLAST | 242 |
Proteomic databases
jPOSTi | P0A794 |
PaxDbi | P0A794 |
PRIDEi | P0A794 |
Interactioni
Subunit structurei
Homooctamer; tetramer of dimers.
2 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260601, 32 interactors |
DIPi | DIP-36215N |
IntActi | P0A794, 5 interactors |
STRINGi | 511145.b2564 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A794 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A794 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 11 – 12 | 1-deoxy-D-xylulose 5-phosphate binding | 2 | |
Regioni | 215 – 216 | 3-amino-2-oxopropyl phosphate binding | 2 |
Sequence similaritiesi
Belongs to the PNP synthase family.Curated
Phylogenomic databases
eggNOGi | COG0854, Bacteria |
HOGENOMi | CLU_074563_0_0_6 |
InParanoidi | P0A794 |
PhylomeDBi | P0A794 |
Family and domain databases
CDDi | cd00003, PNPsynthase, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00279, PdxJ, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR004569, PyrdxlP_synth_PdxJ IPR036130, Pyridoxine-5'_phos_synth |
PANTHERi | PTHR30456, PTHR30456, 1 hit |
Pfami | View protein in Pfam PF03740, PdxJ, 1 hit |
SUPFAMi | SSF63892, SSF63892, 1 hit |
TIGRFAMsi | TIGR00559, pdxJ, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A794-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAELLLGVNI DHIATLRNAR GTAYPDPVQA AFIAEQAGAD GITVHLREDR
60 70 80 90 100
RHITDRDVRI LRQTLDTRMN LEMAVTEEML AIAVETKPHF CCLVPEKRQE
110 120 130 140 150
VTTEGGLDVA GQRDKMRDAC KRLADAGIQV SLFIDADEEQ IKAAAEVGAP
160 170 180 190 200
FIEIHTGCYA DAKTDAEQAQ ELARIAKAAT FAASLGLKVN AGHGLTYHNV
210 220 230 240
KAIAAIPEMH ELNIGHAIIG RAVMTGLKDA VAEMKRLMLE ARG
Sequence cautioni
The sequence D64044 differs from that shown. Reason: Frameshift.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 194 | G → S in pdxH null mutation suppressor. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76470 Genomic DNA Translation: AAA21845.1 M74526 Genomic DNA Translation: AAA24315.1 D64044 Genomic DNA No translation available. U36841 Genomic DNA Translation: AAA79826.1 U00096 Genomic DNA Translation: AAC75617.1 AP009048 Genomic DNA Translation: BAE76740.1 |
PIRi | A42293 |
RefSeqi | NP_417059.1, NC_000913.3 WP_001297412.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75617; AAC75617; b2564 BAE76740; BAE76740; BAE76740 |
GeneIDi | 947039 |
KEGGi | ecj:JW2548 eco:b2564 |
PATRICi | fig|1411691.4.peg.4170 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76470 Genomic DNA Translation: AAA21845.1 M74526 Genomic DNA Translation: AAA24315.1 D64044 Genomic DNA No translation available. U36841 Genomic DNA Translation: AAA79826.1 U00096 Genomic DNA Translation: AAC75617.1 AP009048 Genomic DNA Translation: BAE76740.1 |
PIRi | A42293 |
RefSeqi | NP_417059.1, NC_000913.3 WP_001297412.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HO1 | X-ray | 2.00 | A/B/C/D | 2-243 | [»] | |
1HO4 | X-ray | 2.30 | A/B/C/D | 2-243 | [»] | |
1IXN | X-ray | 2.30 | A/B/C/D | 2-243 | [»] | |
1IXO | X-ray | 2.30 | A/B/C/D | 2-243 | [»] | |
1IXP | X-ray | 2.30 | A/B/C/D | 2-243 | [»] | |
1IXQ | X-ray | 2.30 | A/B/C/D | 2-243 | [»] | |
1M5W | X-ray | 1.96 | A/B/C/D/E/F/G/H | 1-243 | [»] | |
6RG0 | X-ray | 3.07 | A/B/C/D | 2-243 | [»] | |
SMRi | P0A794 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260601, 32 interactors |
DIPi | DIP-36215N |
IntActi | P0A794, 5 interactors |
STRINGi | 511145.b2564 |
Chemistry databases
DrugBanki | DB02496, 1-Deoxy-D-xylulose 5-phosphate DB02209, Pyridoxine phosphate DB02515, sn-glycerol 3-phosphate |
Proteomic databases
jPOSTi | P0A794 |
PaxDbi | P0A794 |
PRIDEi | P0A794 |
Genome annotation databases
EnsemblBacteriai | AAC75617; AAC75617; b2564 BAE76740; BAE76740; BAE76740 |
GeneIDi | 947039 |
KEGGi | ecj:JW2548 eco:b2564 |
PATRICi | fig|1411691.4.peg.4170 |
Organism-specific databases
EchoBASEi | EB0687 |
Phylogenomic databases
eggNOGi | COG0854, Bacteria |
HOGENOMi | CLU_074563_0_0_6 |
InParanoidi | P0A794 |
PhylomeDBi | P0A794 |
Enzyme and pathway databases
UniPathwayi | UPA00244;UER00313 |
BioCyci | EcoCyc:PDXJ-MONOMER MetaCyc:PDXJ-MONOMER |
BRENDAi | 2.6.99.2, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A794 |
PROi | PR:P0A794 |
Family and domain databases
CDDi | cd00003, PNPsynthase, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00279, PdxJ, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR004569, PyrdxlP_synth_PdxJ IPR036130, Pyridoxine-5'_phos_synth |
PANTHERi | PTHR30456, PTHR30456, 1 hit |
Pfami | View protein in Pfam PF03740, PdxJ, 1 hit |
SUPFAMi | SSF63892, SSF63892, 1 hit |
TIGRFAMsi | TIGR00559, pdxJ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDXJ_ECOLI | |
Accessioni | P0A794Primary (citable) accession number: P0A794 Secondary accession number(s): P24223, Q2MAG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families