UniProtKB - P0A784 (ORN_ECOLI)
Protein
Oligoribonuclease
Gene
orn
Organism
Escherichia coli (strain K12)
Status
Functioni
3'-to-5' exoribonuclease specific for small oligoribonucleotides 2 to 5 nucleotides in length, as well as small (2 to 5 nucleotides) ssDNA oligomers. Probably responsible for the final step in mRNA degradation.3 Publications
Activity regulationi
Inhibited by adenosine 3',5'-bisphosphate (PAP).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 12 | Zinc | 1 | |
Metal bindingi | 14 | Zinc | 1 | |
Active sitei | 129 | Sequence analysis | 1 | |
Metal bindingi | 163 | Zinc | 1 |
GO - Molecular functioni
- 3'-5'-exoribonuclease activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- nucleic acid binding Source: InterPro
- oligoribonucleotidase activity Source: EcoCyc
- single-stranded DNA 3'-5' exodeoxyribonuclease activity Source: EcoCyc
GO - Biological processi
- RNA catabolic process Source: EcoCyc
Keywordsi
Molecular function | Exonuclease, Hydrolase, Nuclease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7842-MONOMER MetaCyc:G7842-MONOMER |
BRENDAi | 3.1.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:orn Synonyms:o204a, yjeR Ordered Locus Names:b4162, JW5740 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Essential. In depletion experiments the level of oligonucleotides is elevated and that of mononucleotides is severely reduced compared to wild-type.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000111033 | 2 – 181 | OligoribonucleaseAdd BLAST | 180 |
Proteomic databases
jPOSTi | P0A784 |
PaxDbi | P0A784 |
PRIDEi | P0A784 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261079, 11 interactors |
DIPi | DIP-10412N |
STRINGi | 511145.b4162 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A784 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A784 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 171 | ExonucleaseAdd BLAST | 164 |
Sequence similaritiesi
Belongs to the oligoribonuclease family.Curated
Phylogenomic databases
eggNOGi | COG1949, Bacteria |
HOGENOMi | CLU_064761_2_0_6 |
InParanoidi | P0A784 |
PhylomeDBi | P0A784 |
Family and domain databases
CDDi | cd06135, Orn, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
HAMAPi | MF_00045, Oligoribonuclease, 1 hit |
InterProi | View protein in InterPro IPR013520, Exonuclease_RNaseT/DNA_pol3 IPR022894, Oligoribonuclease IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00929, RNase_T, 1 hit |
SMARTi | View protein in SMART SM00479, EXOIII, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A784-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSANENNLIW IDLEMTGLDP ERDRIIEIAT LVTDANLNIL AEGPTIAVHQ
60 70 80 90 100
SDEQLALMDD WNVRTHTASG LVERVKASTM GDREAELATL EFLKQWVPAG
110 120 130 140 150
KSPICGNSIG QDRRFLFKYM PELEAYFHYR YLDVSTLKEL ARRWKPEILD
160 170 180
GFTKQGTHQA MDDIRESVAE LAYYREHFIK L
Sequence cautioni
The sequence AAA97061 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97061.1 Different initiation. U00096 Genomic DNA Translation: AAC77122.2 AP009048 Genomic DNA Translation: BAE78166.1 |
PIRi | S56390 |
RefSeqi | NP_418586.4, NC_000913.3 WP_001295188.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77122; AAC77122; b4162 BAE78166; BAE78166; BAE78166 |
GeneIDi | 58349692 948675 |
KEGGi | ecj:JW5740 eco:b4162 |
PATRICi | fig|1411691.4.peg.2536 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97061.1 Different initiation. U00096 Genomic DNA Translation: AAC77122.2 AP009048 Genomic DNA Translation: BAE78166.1 |
PIRi | S56390 |
RefSeqi | NP_418586.4, NC_000913.3 WP_001295188.1, NZ_STEB01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YTA | X-ray | 2.20 | A/B/C/D | 2-181 | [»] | |
2IGI | X-ray | 1.70 | A/B | 2-181 | [»] | |
SMRi | P0A784 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261079, 11 interactors |
DIPi | DIP-10412N |
STRINGi | 511145.b4162 |
Proteomic databases
jPOSTi | P0A784 |
PaxDbi | P0A784 |
PRIDEi | P0A784 |
Genome annotation databases
EnsemblBacteriai | AAC77122; AAC77122; b4162 BAE78166; BAE78166; BAE78166 |
GeneIDi | 58349692 948675 |
KEGGi | ecj:JW5740 eco:b4162 |
PATRICi | fig|1411691.4.peg.2536 |
Organism-specific databases
EchoBASEi | EB2373 |
Phylogenomic databases
eggNOGi | COG1949, Bacteria |
HOGENOMi | CLU_064761_2_0_6 |
InParanoidi | P0A784 |
PhylomeDBi | P0A784 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7842-MONOMER MetaCyc:G7842-MONOMER |
BRENDAi | 3.1.13.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A784 |
PROi | PR:P0A784 |
Family and domain databases
CDDi | cd06135, Orn, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
HAMAPi | MF_00045, Oligoribonuclease, 1 hit |
InterProi | View protein in InterPro IPR013520, Exonuclease_RNaseT/DNA_pol3 IPR022894, Oligoribonuclease IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00929, RNase_T, 1 hit |
SMARTi | View protein in SMART SM00479, EXOIII, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ORN_ECOLI | |
Accessioni | P0A784Primary (citable) accession number: P0A784 Secondary accession number(s): P39287, P76799, Q2M6E0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families