UniProtKB - P0A780 (NUSB_ECOLI)
Protein
Transcription antitermination protein NusB
Gene
nusB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed:32871103). Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons (PubMed:7045592, PubMed:7678781, PubMed:11884128, PubMed:14973028, PubMed:15716433, PubMed:16109710). The affinity of NusB for the boxA RNA sequence is significantly increased in the presence of the ribosomal protein S10 (PubMed:16109710, PubMed:11884128). NusB may serve as a loading factor that ensures efficient entry of S10 into the transcription complexes (PubMed:19111659). It also modulates the rrn boxA-mediated transcription elongation rates (PubMed:10383769).9 Publications
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA-templated transcription, termination Source: UniProtKB-UniRule
- transcription antitermination Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | Ribosome biogenesis, Transcription, Transcription antitermination, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10666-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription antitermination protein NusBUniRule annotationCuratedAlternative name(s): Antitermination factor NusB1 PublicationUniRule annotation N utilization substance protein BCurated |
Gene namesi | Name:nusB1 PublicationUniRule annotation Synonyms:groNB1 Publication, ssyB1 Publication Ordered Locus Names:b0416, JW0406 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Inactivation of the gene causes cold-sensitive cell growth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000176536 | 1 – 139 | Transcription antitermination protein NusBAdd BLAST | 139 |
Proteomic databases
jPOSTi | P0A780 |
PaxDbi | P0A780 |
PRIDEi | P0A780 |
Interactioni
Subunit structurei
Monomer in solution (PubMed:10881193, PubMed:16109710).
Forms a heterodimer with the ribosomal protein S10 (PubMed:1731086, PubMed:11884128, PubMed:16109710, PubMed:19111659). The rRNA transcription and antitermination complex (rrnTAC) consists of RNAP, NusA, NusB, NusE (rpsJ), NusG, SubB, ribosomal protein S4, DNA and precursor rRNA; S4 is more flexible than other subunits (PubMed:32871103).
6 PublicationsBinary interactionsi
P0A780
With | #Exp. | IntAct |
---|---|---|
rpsJ [P0A7R5] | 6 | EBI-555387,EBI-544602 |
Protein-protein interaction databases
BioGRIDi | 4259834, 56 interactors |
ComplexPortali | CPX-5674, Transcription elongation complex CPX-5780, lambdaN-dependent processive transcription antitermination complex |
DIPi | DIP-48254N |
IntActi | P0A780, 12 interactors |
STRINGi | 511145.b0416 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A780 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A780 |
Family & Domainsi
Domaini
The N-terminal region functions as an arginine-rich RNA-binding motif (ARM).1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0781, Bacteria |
HOGENOMi | CLU_087843_4_1_6 |
InParanoidi | P0A780 |
PhylomeDBi | P0A780 |
Family and domain databases
Gene3Di | 1.10.940.10, 1 hit |
HAMAPi | MF_00073, NusB, 1 hit |
InterProi | View protein in InterPro IPR035926, NusB-like_sf IPR011605, NusB_fam IPR006027, NusB_RsmB_TIM44 |
PANTHERi | PTHR11078, PTHR11078, 1 hit |
Pfami | View protein in Pfam PF01029, NusB, 1 hit |
SUPFAMi | SSF48013, SSF48013, 1 hit |
TIGRFAMsi | TIGR01951, nusB, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A780-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE
60 70 80 90 100
LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY
110 120 130
KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 59 | T → S (PubMed:6330693).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 18 | Y → D in nusB5; abolishes antitermination. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00681 Genomic DNA Translation: CAA25289.1 X64395 Genomic DNA Translation: CAA45737.1 M26839 Genomic DNA Translation: AAA24228.1 U82664 Genomic DNA Translation: AAB40172.1 U00096 Genomic DNA Translation: AAC73519.1 AP009048 Genomic DNA Translation: BAE76196.1 |
PIRi | I51822, FJECB |
RefSeqi | NP_414950.1, NC_000913.3 WP_000801125.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73519; AAC73519; b0416 BAE76196; BAE76196; BAE76196 |
GeneIDi | 52075757 945054 |
KEGGi | ecj:JW0406 eco:b0416 |
PATRICi | fig|1411691.4.peg.1861 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00681 Genomic DNA Translation: CAA25289.1 X64395 Genomic DNA Translation: CAA45737.1 M26839 Genomic DNA Translation: AAA24228.1 U82664 Genomic DNA Translation: AAB40172.1 U00096 Genomic DNA Translation: AAC73519.1 AP009048 Genomic DNA Translation: BAE76196.1 |
PIRi | I51822, FJECB |
RefSeqi | NP_414950.1, NC_000913.3 WP_000801125.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EY1 | NMR | - | A | 1-139 | [»] | |
3D3B | X-ray | 1.30 | A | 1-139 | [»] | |
3D3C | X-ray | 2.60 | A/B/C | 1-139 | [»] | |
3IMQ | X-ray | 2.50 | A/B/C | 3-139 | [»] | |
5MS0 | electron microscopy | 9.80 | L | 1-139 | [»] | |
6TQN | electron microscopy | 3.80 | B | 1-139 | [»] | |
6TQO | electron microscopy | 4.00 | B | 1-139 | [»] | |
SMRi | P0A780 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259834, 56 interactors |
ComplexPortali | CPX-5674, Transcription elongation complex CPX-5780, lambdaN-dependent processive transcription antitermination complex |
DIPi | DIP-48254N |
IntActi | P0A780, 12 interactors |
STRINGi | 511145.b0416 |
Proteomic databases
jPOSTi | P0A780 |
PaxDbi | P0A780 |
PRIDEi | P0A780 |
Genome annotation databases
EnsemblBacteriai | AAC73519; AAC73519; b0416 BAE76196; BAE76196; BAE76196 |
GeneIDi | 52075757 945054 |
KEGGi | ecj:JW0406 eco:b0416 |
PATRICi | fig|1411691.4.peg.1861 |
Organism-specific databases
EchoBASEi | EB0660 |
Phylogenomic databases
eggNOGi | COG0781, Bacteria |
HOGENOMi | CLU_087843_4_1_6 |
InParanoidi | P0A780 |
PhylomeDBi | P0A780 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10666-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A780 |
PROi | PR:P0A780 |
Family and domain databases
Gene3Di | 1.10.940.10, 1 hit |
HAMAPi | MF_00073, NusB, 1 hit |
InterProi | View protein in InterPro IPR035926, NusB-like_sf IPR011605, NusB_fam IPR006027, NusB_RsmB_TIM44 |
PANTHERi | PTHR11078, PTHR11078, 1 hit |
Pfami | View protein in Pfam PF01029, NusB, 1 hit |
SUPFAMi | SSF48013, SSF48013, 1 hit |
TIGRFAMsi | TIGR01951, nusB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUSB_ECOLI | |
Accessioni | P0A780Primary (citable) accession number: P0A780 Secondary accession number(s): P04381, Q2MC10 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | June 7, 2005 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families